(Circular) (Six-base) MAPSORT of: pFN21AE2387.seq Check: 1945 from: 1 to: 9973 pFN21AE2387 9973 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3154 4144 6972 3154 Size: 990 2828 6155 Fragments arranged by size: 6155 2828 990 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6609 7783 278 Size: 53 83 186 936 5073 1174 2468 Fragments arranged by size: 5073 2468 1174 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7316 1937 Size: 5379 4594 Acc65I G'GTAC_C Cuts at: 3066 7294 3066 Size: 4228 5745 AclI AA'CG_TT Cuts at: 1563 8100 8473 1563 Size: 6537 373 3063 Fragments arranged by size: 6537 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2002 2354 3497 4325 4566 5151 5732 8036 Size: 370 352 1143 828 241 585 581 2304 Cuts at: 8036 9420 9688 1632 Size: 1384 268 1917 Fragments arranged by size: 2304 1917 1384 1143 828 585 581 370 352 268 241 AfeI AGC'GCT Cuts at: 2666 3319 6291 2666 Size: 653 2972 6348 Fragments arranged by size: 6348 2972 653 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9776 AflIII A'CryG_T Cuts at: 1203 7053 8872 9849 1203 Size: 5850 1819 977 1327 Fragments arranged by size: 5850 1819 1327 977 AgeI A'CCGG_T Cuts at: 3063 8804 3063 Size: 5741 4232 AhdI GACnn_n'nnGTC Cuts at: 7144 8702 7144 Size: 1558 8415 AleI CACnn'nnGTG Cuts at: 1166 4505 1166 Size: 3339 6634 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2130 3645 4956 1184 Size: 946 1515 1311 6201 Fragments arranged by size: 6201 1515 1311 946 AlwNI CAG_nnn'CTG Cuts at: 2605 2638 2845 3007 3145 3250 3374 3526 3814 Size: 33 207 162 138 105 124 152 288 Cuts at: 3814 3929 3967 4573 4582 4861 4903 5221 5839 Size: 115 38 606 9 279 42 318 618 Cuts at: 5839 6283 6430 7246 7716 9288 2605 Size: 444 147 816 470 1572 3290 Fragments arranged by size: 3290 1572 816 618 606 470 444 318 288 279 207 162 152 147 138 124 115 105 42 38 33 9 ApaI G_GGCC'C Cuts at: 2544 5020 2544 Size: 2476 7497 ApaLI G'TGCA_C Cuts at: 2897 4115 8030 9186 2897 Size: 1218 3915 1156 3684 Fragments arranged by size: 3915 3684 1218 1156 ApoI r'AATT_y Cuts at: 1493 2773 6647 7282 7508 7660 9769 1493 Size: 1280 3874 635 226 152 2109 1697 Fragments arranged by size: 3874 2109 1697 1280 635 226 152 AseI AT'TA_AT Cuts at: 160 1032 8527 160 Size: 872 7495 1606 Fragments arranged by size: 7495 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9973 AvaI C'yCGr_G Cuts at: 1870 1943 2861 4051 5082 6443 6896 7298 1870 Size: 73 918 1190 1031 1361 453 402 4545 Fragments arranged by size: 4545 1361 1190 1031 918 453 402 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6254 6287 6254 Size: 33 9940 BamHI G'GATC_C Cuts at: 2401 3585 4831 7303 7670 2401 Size: 1184 1246 2472 367 4704 Fragments arranged by size: 4704 2472 1246 1184 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2760 3066 3169 3746 4825 Size: 325 217 627 973 306 103 577 1079 Cuts at: 4825 5078 5615 7294 8749 618 Size: 253 537 1679 1455 1842 Fragments arranged by size: 1842 1679 1455 1079 973 627 577 537 325 306 253 217 103 BanII G_rGCy'C Cuts at: 1391 2085 2544 3309 4266 5020 6033 6444 6929 Size: 694 459 765 957 754 1013 411 485 Cuts at: 6929 7041 7292 1391 Size: 112 251 4072 Fragments arranged by size: 4072 1013 957 765 754 694 485 459 411 251 112 BbeI G_GCGC'C Cuts at: 2764 5082 2764 Size: 2318 7655 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2991 3485 3698 5462 5910 928 Size: 971 1092 494 213 1764 448 4991 Fragments arranged by size: 4991 1764 1092 971 494 448 213 BbvCI CC'TCA_GC Cuts at: 3708 3761 4283 6309 3708 Size: 53 522 2026 7372 Fragments arranged by size: 7372 2026 522 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2171 2205 6092 6126 8164 8198 1903 Size: 34 234 34 3887 34 2038 34 3678 Fragments arranged by size: 3887 3678 2038 234 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7865 9075 7865 Size: 1210 8763 BclI T'GATC_A Cuts at: 1548 1548 Size: 9973 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3638 5087 5126 Size: 107 122 71 631 605 1966 1449 39 Cuts at: 5126 8584 136 Size: 3458 1525 Fragments arranged by size: 3458 1966 1525 1449 631 605 122 107 71 39 BglII A'GATC_T Cuts at: 2373 4362 5808 7095 9968 2373 Size: 1989 1446 1287 2873 2378 Fragments arranged by size: 2873 2378 1989 1446 1287 BlpI GC'TnA_GC Cuts at: 5276 7390 5276 Size: 2114 7859 Bme1580I G_kGCm'C Cuts at: 1790 2544 2901 3174 4119 4945 5020 8034 9190 Size: 754 357 273 945 826 75 3014 1156 Cuts at: 9190 1790 Size: 2573 Fragments arranged by size: 3014 2573 1156 945 826 754 357 273 75 BmgBI CAC'GTC Cuts at: 1318 2212 2989 3133 1318 Size: 894 777 144 8158 Fragments arranged by size: 8158 894 777 144 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2425 4248 7096 Size: 416 53 83 399 24 1008 1823 2848 Cuts at: 7096 7704 8662 442 Size: 608 958 1753 Fragments arranged by size: 2848 1823 1753 1008 958 608 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5445 1056 Size: 4389 5584 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4502 6049 7114 4502 Size: 1547 1065 7361 Fragments arranged by size: 7361 1547 1065 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2746 2813 3623 3875 4115 4604 4686 4751 Size: 1507 67 810 252 240 489 82 65 Cuts at: 4751 4862 4937 4967 5075 5222 5270 5603 5672 Size: 111 75 30 108 147 48 333 69 Cuts at: 5672 5999 6233 6314 6380 6452 6494 6593 6734 Size: 327 234 81 66 72 42 99 141 Cuts at: 6734 6755 6817 6869 8633 1239 Size: 21 62 52 1764 2579 Fragments arranged by size: 2579 1764 1507 810 489 333 327 252 240 234 147 141 111 108 99 82 81 75 72 69 67 66 65 62 52 48 42 30 21 Bpu10I CC'TnA_GC Cuts at: 2199 3000 3708 3761 4283 5252 5756 6309 2199 Size: 801 708 53 522 969 504 553 5863 Fragments arranged by size: 5863 969 801 708 553 522 504 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2936 3161 3185 3635 4792 4802 5282 5342 6221 Size: 225 24 450 1157 10 480 60 879 Cuts at: 6221 6545 6854 8098 8963 9261 2936 Size: 324 309 1244 865 298 3648 Fragments arranged by size: 3648 1244 1157 879 865 480 450 324 309 298 225 60 24 10 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4289 4437 4631 4949 6440 6731 6780 Size: 617 2790 148 194 318 1491 291 49 Cuts at: 6780 8636 882 Size: 1856 2219 Fragments arranged by size: 2790 2219 1856 1491 617 318 291 194 148 49 BsaAI yAC'GTr Cuts at: 493 1204 4228 4468 4981 7764 493 Size: 711 3024 240 513 2783 2702 Fragments arranged by size: 3024 2783 2702 711 513 240 BsaBI GATnn'nnATC Cuts at: 9967 9967 Size: 9973 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2761 5079 5930 6606 Size: 53 83 186 936 1228 2318 851 676 Cuts at: 6606 7780 8162 8797 275 Size: 1174 382 635 1451 Fragments arranged by size: 2318 1451 1228 1174 936 851 676 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2784 3063 3414 3786 5631 6453 8406 8804 9078 Size: 279 351 372 1845 822 1953 398 274 Cuts at: 9078 9225 9636 2784 Size: 147 411 3121 Fragments arranged by size: 3121 1953 1845 822 411 398 372 351 279 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4664 4694 5195 5225 5591 Size: 30 165 30 3261 30 501 30 366 Cuts at: 5591 5621 5915 5945 6815 6845 1178 Size: 30 294 30 870 30 4306 Fragments arranged by size: 4306 3261 870 501 366 294 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 2516 2819 2954 4592 4688 4721 4859 Size: 1261 69 303 135 1638 96 33 138 Cuts at: 4859 4895 4925 5063 5090 5282 5285 5504 5507 Size: 36 30 138 27 192 3 219 3 Cuts at: 5507 5636 5777 5936 5969 6038 6041 6095 6098 Size: 129 141 159 33 69 3 54 3 Cuts at: 6098 6230 6590 6593 7106 1186 Size: 132 360 3 513 4053 Fragments arranged by size: 4053 1638 1261 513 360 303 219 192 159 141 138 138 135 132 129 96 69 69 54 36 33 33 30 27 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2750 3300 3320 3437 3876 4123 4377 5196 9176 Size: 550 20 117 439 247 254 819 3980 Cuts at: 9176 9672 2750 Size: 496 3051 Fragments arranged by size: 3980 3051 819 550 496 439 254 247 117 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2075 2653 2807 3158 3641 5534 6077 Size: 573 339 578 154 351 483 1893 543 Cuts at: 6077 6453 1163 Size: 376 4683 Fragments arranged by size: 4683 1893 578 573 543 483 376 351 339 154 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2853 3345 7423 8184 9212 1540 Size: 275 186 852 492 4078 761 1028 2301 Fragments arranged by size: 4078 2301 1028 852 761 492 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2205 2859 2901 3309 3704 3801 4052 4058 Size: 120 654 42 408 395 97 251 6 Cuts at: 4058 4083 4119 4206 4751 4818 5100 5358 6033 Size: 25 36 87 545 67 282 258 675 Cuts at: 6033 6444 6507 8034 8119 9190 2085 Size: 411 63 1527 85 1071 2868 Fragments arranged by size: 2868 1527 1071 675 654 545 411 408 395 282 258 251 120 97 87 85 67 63 42 36 25 6 BsmI GAATG_Cn' Cuts at: 2119 7484 7577 2119 Size: 5365 93 4515 Fragments arranged by size: 5365 4515 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4956 5937 4956 Size: 981 8992 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2205 2544 2859 2901 3174 3309 Size: 399 295 120 339 315 42 273 135 Cuts at: 3309 3704 3801 4052 4058 4083 4119 4206 4266 Size: 395 97 251 6 25 36 87 60 Cuts at: 4266 4751 4818 4945 5020 5100 5358 6033 6444 Size: 485 67 127 75 80 258 675 411 Cuts at: 6444 6507 6929 7041 7292 8034 8119 9190 1391 Size: 63 422 112 251 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 675 485 422 411 399 395 339 315 295 273 258 251 251 135 127 120 112 97 87 85 80 75 67 63 60 42 36 25 6 BspEI T'CCGG_A Cuts at: 5631 6453 9636 5631 Size: 822 3183 5968 Fragments arranged by size: 5968 3183 822 BspHI T'CATG_A Cuts at: 7862 9592 7862 Size: 1730 8243 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 2484 2934 3154 4144 4152 4329 6735 Size: 1517 123 450 220 990 8 177 2406 Cuts at: 6735 6798 6972 7328 844 Size: 63 174 356 3489 Fragments arranged by size: 3489 2406 1517 990 450 356 220 177 174 123 63 8 BsrBI CCG'CTC Cuts at: 1394 1823 3199 3637 4717 5317 6205 6232 7860 Size: 429 1376 438 1080 600 888 27 1628 Cuts at: 7860 1394 Size: 3507 Fragments arranged by size: 3507 1628 1376 1080 888 600 438 429 27 BsrDI GCAATG_nn' Cuts at: 65 1248 2274 8468 8642 65 Size: 1183 1026 6194 174 1396 Fragments arranged by size: 6194 1396 1183 1026 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2757 3063 3561 6186 6417 6618 8617 Size: 110 947 306 498 2625 231 201 1999 Cuts at: 8617 8804 1700 Size: 187 2869 Fragments arranged by size: 2869 2625 1999 947 498 306 231 201 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9973 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9973 BssSI C'ACGA_G Cuts at: 3896 6783 8033 9045 3896 Size: 2887 1250 1012 4824 Fragments arranged by size: 4824 2887 1250 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3145 3169 3805 4030 4529 6385 794 Size: 2351 24 636 225 499 1856 4382 Fragments arranged by size: 4382 2351 1856 636 499 225 24 BstBI TT'CG_AA Cuts at: 8777 8777 Size: 9973 BstEII G'GTnAC_C Cuts at: 1184 6325 1184 Size: 5141 4832 BstXI CCAn_nnnn'nTGG Cuts at: 2465 3044 3214 3556 3853 5509 5875 2465 Size: 579 170 342 297 1656 366 6563 Fragments arranged by size: 6563 1656 579 366 342 297 170 BstYI r'GATC_y Cuts at: 1972 2373 2401 3585 4362 4831 5808 5886 5974 Size: 401 28 1184 777 469 977 78 88 Cuts at: 5974 6549 7095 7303 7670 8055 8072 9524 9610 Size: 575 546 208 367 385 17 1452 86 Cuts at: 9610 9622 9968 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1184 977 777 575 546 469 401 385 367 346 208 88 86 78 28 17 12 Bsu36I CC'TnA_GG Cuts at: 3375 4788 5660 3375 Size: 1413 872 7688 Fragments arranged by size: 7688 1413 872 BtgI C'CryG_G Cuts at: 513 1065 3213 3975 4213 5322 6804 6930 513 Size: 552 2148 762 238 1109 1482 126 3556 Fragments arranged by size: 3556 2148 1482 1109 762 552 238 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9234 Fragments arranged by size: 9234 683 56 BtsI GCAGTG_nn' Cuts at: 3748 4052 4081 4922 7489 8283 8303 3748 Size: 304 29 841 2567 794 20 5418 Fragments arranged by size: 5418 2567 841 794 304 29 20 ClaI AT'CG_AT Cuts at: 1554 6129 1554 Size: 4575 5398 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9938 DraI TTT'AAA Cuts at: 7277 7277 Size: 9973 DraIII CAC_nnn'GTG Cuts at: 2497 3227 2497 Size: 730 9243 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7217 8980 809 Size: 507 5901 1763 1802 Fragments arranged by size: 5901 1802 1763 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2550 2850 Size: 54 1425 50 275 18 441 279 300 Cuts at: 2850 3342 3546 3593 3843 3888 3972 4038 4062 Size: 492 204 47 250 45 84 66 24 Cuts at: 4062 4216 5118 5325 5376 5540 6933 7420 8309 Size: 154 902 207 51 164 1393 487 889 Cuts at: 8309 9938 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1393 902 889 492 487 441 300 279 275 250 207 204 164 154 84 66 54 51 50 47 45 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2850 3342 7420 1537 Size: 275 1038 492 4078 4090 Fragments arranged by size: 4090 4078 1038 492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2021 3854 4440 4509 5127 5132 5997 Size: 665 3 1833 586 69 618 5 865 Cuts at: 5997 6402 6687 7164 7903 1353 Size: 405 285 477 739 3423 Fragments arranged by size: 3423 1833 865 739 665 618 586 477 405 285 69 5 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2279 2315 5039 5567 5570 6497 Size: 1064 138 690 36 2724 528 3 927 Cuts at: 6497 6824 8560 8934 9080 387 Size: 327 1736 374 146 1280 Fragments arranged by size: 2724 1736 1280 1064 927 690 528 374 327 146 138 36 3 EcoICRI GAG'CTC Cuts at: 2083 3307 6031 6442 2083 Size: 1224 2724 411 5614 Fragments arranged by size: 5614 2724 1224 411 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2354 2746 2813 3497 3623 3875 Size: 393 370 352 392 67 684 126 252 Cuts at: 3875 4115 4325 4566 4604 4686 4751 4862 4937 Size: 240 210 241 38 82 65 111 75 Cuts at: 4937 4967 5075 5151 5222 5270 5603 5672 5732 Size: 30 108 76 71 48 333 69 60 Cuts at: 5732 5999 6233 6314 6380 6452 6494 6593 6734 Size: 267 234 81 66 72 42 99 141 Cuts at: 6734 6755 6817 6869 8036 8633 9420 9688 1239 Size: 21 62 52 1167 597 787 268 1524 Fragments arranged by size: 1524 1167 787 684 597 393 392 370 352 333 268 267 252 241 240 234 210 141 126 111 108 99 82 81 76 75 72 71 69 67 66 65 62 60 52 48 42 38 30 21 EcoNI CCTnn'n_nnAGG Cuts at: 2222 2552 4013 4949 6575 6996 2222 Size: 330 1461 936 1626 421 5199 Fragments arranged by size: 5199 1626 1461 936 421 330 EcoO109I rG'GnC_Cy Cuts at: 2541 3247 3379 3646 3730 3765 4784 4993 5016 Size: 706 132 267 84 35 1019 209 23 Cuts at: 5016 5656 5672 5836 6358 7109 7174 2541 Size: 640 16 164 522 751 65 5340 Fragments arranged by size: 5340 1019 751 706 640 522 267 209 164 132 84 65 35 23 16 EcoRI G'AATT_C Cuts at: 6647 7282 7660 6647 Size: 635 378 8960 Fragments arranged by size: 8960 635 378 EcoRV GAT'ATC Cuts at: 2323 2323 Size: 9973 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4544 6650 4544 Size: 2106 7867 FspI TGC'GCA Cuts at: 3014 5000 8479 3014 Size: 1986 3479 4508 Fragments arranged by size: 4508 3479 1986 HaeII r_GCGC'y Cuts at: 1678 2668 2764 3321 4102 4188 5082 5242 6187 Size: 990 96 557 781 86 894 160 945 Cuts at: 6187 6293 6922 6973 9120 1678 Size: 106 629 51 2147 2531 Fragments arranged by size: 2531 2147 990 945 894 781 629 557 160 106 96 86 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3472 3504 5915 5947 7130 Size: 32 135 32 1900 32 2411 32 1183 Cuts at: 7130 7162 7186 7218 8614 8646 8688 8720 1373 Size: 32 24 32 1396 32 42 32 2626 Fragments arranged by size: 2626 2411 1900 1396 1183 135 42 32 32 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 2413 7222 7317 7563 669 Size: 988 281 475 4809 95 246 3079 Fragments arranged by size: 4809 3079 988 475 281 246 95 HindIII A'AGCT_T Cuts at: 748 748 Size: 9973 HpaI GTT'AAC Cuts at: 7563 7563 Size: 9973 KasI G'GCGC_C Cuts at: 2760 5078 2760 Size: 2318 7655 KpnI G_GTAC'C Cuts at: 3070 7298 3070 Size: 4228 5745 MfeI C'AATT_G Cuts at: 7572 7572 Size: 9973 MluI A'CGCG_T Cuts at: 9849 9849 Size: 9973 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 6415 9087 9271 1305 Size: 906 4204 2672 184 2007 Fragments arranged by size: 4204 2672 2007 906 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2552 3548 3845 3890 4064 Size: 54 1425 343 720 996 297 45 174 Cuts at: 4064 10 Size: 5919 Fragments arranged by size: 5919 1425 996 720 343 297 174 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 3212 4505 6131 6803 7058 7931 8290 Size: 648 2046 1293 1626 672 255 873 359 Cuts at: 8290 8449 518 Size: 159 2042 Fragments arranged by size: 2046 2042 1626 1293 873 672 648 359 255 159 MspA1I CmG'CkG Cuts at: 1606 2104 2332 2482 2842 3437 3463 3523 3811 Size: 498 228 150 360 595 26 60 288 Cuts at: 3811 4215 4729 4861 4867 4900 4930 4936 5041 Size: 404 514 132 6 33 30 6 105 Cuts at: 5041 5200 5221 5752 5818 6211 6268 6280 6400 Size: 159 21 531 66 393 57 12 120 Cuts at: 6400 6712 6733 6952 7389 8066 9214 9459 1606 Size: 312 21 219 437 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 595 531 514 498 437 404 393 360 312 288 245 228 219 159 150 132 120 105 66 60 57 33 30 26 21 21 12 6 6 NaeI GCC'GGC Cuts at: 2759 6188 6620 2759 Size: 3429 432 6112 Fragments arranged by size: 6112 3429 432 NarI GG'CG_CC Cuts at: 2761 5079 2761 Size: 2318 7655 NcoI C'CATG_G Cuts at: 513 1065 3975 5322 513 Size: 552 2910 1347 5164 Fragments arranged by size: 5164 2910 1347 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9973 NgoMIV G'CCGG_C Cuts at: 2757 6186 6618 2757 Size: 3429 432 6112 Fragments arranged by size: 6112 3429 432 NheI G'CTAG_C Cuts at: 1052 5441 1052 Size: 4389 5584 NotI GC'GGCC_GC Cuts at: 7420 7420 Size: 9973 NspI r_CATG'y Cuts at: 1132 1229 2662 2716 6383 7057 7331 8876 1132 Size: 97 1433 54 3667 674 274 1545 2229 Fragments arranged by size: 3667 2229 1545 1433 674 274 97 54 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9973 PciI A'CATG_T Cuts at: 7053 8872 7053 Size: 1819 8154 PflMI CCAn_nnn'nTGG Cuts at: 1108 2497 2845 3166 3743 4156 6840 7246 1108 Size: 1389 348 321 577 413 2684 406 3835 Fragments arranged by size: 3835 2684 1389 577 413 406 348 321 PfoI T'CCnGG_A Cuts at: 3490 3945 4074 5286 5880 6429 7239 3490 Size: 455 129 1212 594 549 810 6224 Fragments arranged by size: 6224 1212 810 594 549 455 129 PmeI GTTT'AAAC Cuts at: 7277 7277 Size: 9973 PmlI CAC'GTG Cuts at: 4228 4468 4228 Size: 240 9733 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8042 8074 9555 9587 8042 Size: 32 1481 32 8428 Fragments arranged by size: 8428 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3646 3765 4993 6358 7109 3646 Size: 119 1228 1365 751 6510 Fragments arranged by size: 6510 1365 1228 751 119 PshAI GACnn'nnGTC Cuts at: 1613 6841 1613 Size: 5228 4745 PsiI TTA'TAA Cuts at: 7543 7543 Size: 9973 PspOMI G'GGCC_C Cuts at: 2540 5016 2540 Size: 2476 7497 PspXI vC'TCGA_Gb Cuts at: 4051 4051 Size: 9973 PstI C_TGCA'G Cuts at: 830 2109 2184 2223 2652 3150 3282 3459 3810 Size: 1279 75 39 429 498 132 177 351 Cuts at: 3810 3870 4236 4578 4866 4908 4929 4935 5109 Size: 60 366 342 288 42 21 6 174 Cuts at: 5109 5187 5226 6183 6354 6732 6915 7182 7325 Size: 78 39 957 171 378 183 267 143 Cuts at: 7325 830 Size: 3478 Fragments arranged by size: 3478 1279 957 498 429 378 366 351 342 288 267 183 177 174 171 143 132 78 75 60 42 39 39 21 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9973 PvuII CAG'CTG Cuts at: 2104 2332 2842 3437 3523 3811 4729 4861 4900 Size: 228 510 595 86 288 918 132 39 Cuts at: 4900 4930 4936 5041 5200 5221 5818 6211 6400 Size: 30 6 105 159 21 597 393 189 Cuts at: 6400 6712 6733 2104 Size: 312 21 5344 Fragments arranged by size: 5344 918 597 595 510 393 312 288 228 189 159 132 105 86 39 30 21 21 6 SacI G_AGCT'C Cuts at: 2085 3309 6033 6444 2085 Size: 1224 2724 411 5614 Fragments arranged by size: 5614 2724 1224 411 SacII CC_GC'GG Cuts at: 4216 4216 Size: 9973 SalI G'TCGA_C Cuts at: 1936 7315 1936 Size: 5379 4594 SanDI GG'GwC_CC Cuts at: 7109 7109 Size: 9973 SapI GCTCTTCn'nnn_ Cuts at: 2021 3854 5132 5997 6402 2021 Size: 1833 1278 865 405 5592 Fragments arranged by size: 5592 1833 1278 865 405 SbfI CC_TGCA'GG Cuts at: 7325 7325 Size: 9973 ScaI AGT'ACT Cuts at: 8221 9933 8221 Size: 1712 8261 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 9973 SfcI C'TryA_G Cuts at: 826 1045 2105 2180 2219 2377 2648 3146 3265 Size: 219 1060 75 39 158 271 498 119 Cuts at: 3265 3278 3455 3509 3806 3866 4178 4232 4574 Size: 13 177 54 297 60 312 54 342 Cuts at: 4574 4862 4904 4925 4931 5105 5183 5222 6179 Size: 288 42 21 6 174 78 39 957 Cuts at: 6179 6350 6728 6911 7178 7321 8456 9137 9328 Size: 171 378 183 267 143 1135 681 191 Cuts at: 9328 826 Size: 1471 Fragments arranged by size: 1471 1135 1060 957 681 498 378 342 312 297 288 271 267 219 191 183 177 174 171 158 143 119 78 75 60 54 54 42 39 39 21 13 6 SfoI GGC'GCC Cuts at: 2762 5080 2762 Size: 2318 7655 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9973 SgrAI Cr'CCGG_yG Cuts at: 1700 2757 6186 6417 1700 Size: 1057 3429 231 5256 Fragments arranged by size: 5256 3429 1057 231 SmaI CCC'GGG Cuts at: 1872 2863 5084 7300 1872 Size: 991 2221 2216 4545 Fragments arranged by size: 4545 2221 2216 991 SmlI C'TyrA_G Cuts at: 820 1017 1943 2951 3176 3200 3650 4051 4771 Size: 197 926 1008 225 24 450 401 720 Cuts at: 4771 4817 5297 5357 6200 6443 6560 6869 6896 Size: 46 480 60 843 243 117 309 27 Cuts at: 6896 8077 8978 9240 820 Size: 1181 901 262 1553 Fragments arranged by size: 1553 1181 1008 926 901 843 720 480 450 401 309 262 243 225 197 117 60 46 27 24 SnaBI TAC'GTA Cuts at: 493 7764 493 Size: 7271 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9973 SphI G_CATG'C Cuts at: 1132 2716 6383 7331 1132 Size: 1584 3667 948 3774 Fragments arranged by size: 3774 3667 1584 948 SspI AAT'ATT Cuts at: 5 52 7897 5 Size: 47 7845 2081 Fragments arranged by size: 7845 2081 47 StuI AGG'CCT Cuts at: 1517 6093 1517 Size: 4576 5397 StyI C'CwwG_G Cuts at: 513 1065 2553 2703 3642 3975 4188 5322 5430 Size: 552 1488 150 939 333 213 1134 108 Cuts at: 5430 5484 5550 5916 6669 6882 7412 513 Size: 54 66 366 753 213 530 3074 Fragments arranged by size: 3074 1488 1134 939 753 552 530 366 333 213 213 150 108 66 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2310 3497 4001 4766 8000 8017 8170 Size: 1255 730 1187 504 765 3234 17 153 Cuts at: 8170 8355 8800 325 Size: 185 445 1498 Fragments arranged by size: 3234 1498 1255 1187 765 730 504 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2682 2955 4354 Size: 274 80 33 51 1443 705 273 1399 Cuts at: 4354 5715 8219 9931 96 Size: 1361 2504 1712 138 Fragments arranged by size: 2504 1712 1443 1399 1361 705 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4664 4696 9933 9965 4664 Size: 32 5237 32 4672 Fragments arranged by size: 5237 4672 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9973 XbaI T'CTAG_A Cuts at: 7309 7309 Size: 9973 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2412 5923 7243 2412 Size: 3511 1320 5142 Fragments arranged by size: 5142 3511 1320 XhoI C'TCGA_G Cuts at: 1943 4051 6443 6896 1943 Size: 2108 2392 453 5020 Fragments arranged by size: 5020 2392 2108 453 XmaI C'CCGG_G Cuts at: 1870 2861 5082 7298 1870 Size: 991 2221 2216 4545 Fragments arranged by size: 4545 2221 2216 991 XmnI GAAnn'nnTTC Cuts at: 7055 8102 7055 Size: 1047 8926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6607 7781 276 Size: 53 83 186 936 5073 1174 2468 Fragments arranged by size: 5073 2468 1174 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BsiWI BstZ17I FseI FspAI NruI NsiI PacI PsrI RsrII SfiI SrfI SwaI