RXFP4 ORF clone pFN21AE2255

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Halotag ORF Clone pFN21AE2255

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Clone info.

Species

human

Clone Name

pFN21AE2255 Sequence (with vector) Restriction Map

Product ID

FHC20620

ORF Sequence

DNA sequence (1122 bp)
Protein sequence (374 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2255

Symbol

RXFP4

Alias : GPCR142, GPR100, RLN3R2, RXFPR4

Description

relaxin/insulin-like family peptide receptor 4

Original Clone ID

cs03355

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1122 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 374 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1124.1 4.1e-136 99.7 RXFP4 CCDS3137.1 4.5e-23 28.4 AGTR1 CCDS82855.1 4.8e-23 28.4 AGTR1 CCDS82854.1 4.8e-23 28.4 AGTR1 CCDS14569.1 3.7e-22 31.4 AGTR2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 41 65 PR00237 GPCR IPR000248 45 53 PR00241 Angiotensin II receptor IPR000276 76 97 PR00237 GPCR IPR000248 95 107 PR00241 Angiotensin II receptor IPR000276 121 143 PR00237 GPCR IPR000248 130 137 PR00241 Angiotensin II receptor IPR000276 157 178 PR00237 GPCR IPR000276 205 228 PR00237 GPCR IPR000248 220 231 PR00241 Angiotensin II receptor IPR000276 244 268 PR00237 GPCR IPR000248 261 271 PR00241 Angiotensin II receptor IPR000276 291 317 PR00237 GPCR HMMPfam IPR000276 56 309 PF00001 GPCR ProfileScan IPR017452 56 309 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 39 RLMVALAYGLVGAIGLLGNLAVL 61 PRIMARY 23 2 75 PSDTFVFNLALADLGLALTLPFW 97 SECONDARY 23 3 119 TATVLNVYASIFLITALSVARYW 141 PRIMARY 23 4 165 VWAAAALVTVPTAVFGVEGEVCG 187 PRIMARY 23 5 209 VVLAFMVPLGVITTSYLLLLAFL 231 PRIMARY 23 6 249 RILVASFFLCWFPNHVVTLWGVL 271 SECONDARY 23 7 276 LVPWNSTFYTIQTYVFPVTTCLA 298 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_871001 374 609043 99.7 100.0 XP_011535835 363 300034 31.4 86.4 NP_000677 363 300034 31.4 86.4 NP_001043 388 182454 32.2 86.1 NP_000902 372 165195 31.7 83.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2255 Download

>KIEE2255  1122 bp
ATGCCCACACTCAATACTTCTGCCTCTCCACCCACATTCTTCTGGGCCAATGCCTCCGGA
GGCAGTGTGCTGAGTGCTGATGATGCTCCGATGCCTGTCAAATTCCTAGCCCTGAGGCTC
ATGGTTGCCCTGGCCTATGGGCTTGTGGGGGCCATTGGCTTGCTGGGAAATTTGGCGGTG
CTGTGGGTACTGAGTAACTGTGCCCGGAGAGCCCCTGGCCCACCTTCAGACACCTTCGTC
TTCAACCTGGCTCTGGCGGACCTGGGACTGGCACTCACTCTCCCCTTTTGGGCAGCCGAG
TCGGCACTGGACTTTCACTGGCCCTTCGGAGGTGCCCTCTGCAAGATGGTTCTGACGGCC
ACTGTCCTCAACGTCTATGCCAGCATCTTCCTCATCACAGCGCTGAGCGTTGCTCGCTAC
TGGGTGGTGGCCATGGCTGCGGGGCCAGGCACCCACCTCTCACTCTTCTGGGCCCGAATA
GCCACCCTGGCAGTGTGGGCGGCGGCTGCCCTGGTGACGGTGCCCACAGCTGTCTTCGGG
GTGGAGGGTGAGGTGTGTGGTGTGCGCCTTTGCCTGCTGCGTTTCCCCAGCAGGTACTGG
CTGGGGGCCTACCAGCTGCAGAGGGTGGTGCTGGCTTTCATGGTGCCCTTGGGCGTCATC
ACCACCAGCTACCTGCTGCTGCTGGCCTTCCTGCAGCGGCGGCAACGGCGGCGGCAGGAC
AGCAGGGTCGTGGCCCGCTCTGTCCGCATCCTGGTGGCTTCCTTCTTCCTCTGCTGGTTT
CCCAACCATGTGGTCACTCTCTGGGGTGTCCTGGTGAAGTTTGACCTGGTGCCCTGGAAC
AGTACTTTCTATACTATCCAGACGTATGTCTTCCCTGTCACTACTTGCTTGGCACACAGC
AATAGCTGCCTCAACCCTGTGCTGTACTGTCTCCTGAGGCGGGAGCCCCGGCAGGCTCTG
GCAGGCACCTTCAGGGATCTGCGGTCGAGGCTGTGGCCCCAGGGCGGAGGCTGGGTGCAA
CAGGTGGCCCTAAAGCAGGTAGGCAGGCGGTGGGTCGCAAGCAACCCCCGGGAGAGCCGC
CCTTCTACCCTGCTCACCAACCTGGACAGAGGGACACCCGGG

ORF Protein

Cloned ORF protein sequence for pFN21AE2255 Download

>KIEE2255  374 aa
MPTLNTSASPPTFFWANASGGSVLSADDAPMPVKFLALRLMVALAYGLVGAIGLLGNLAV
LWVLSNCARRAPGPPSDTFVFNLALADLGLALTLPFWAAESALDFHWPFGGALCKMVLTA
TVLNVYASIFLITALSVARYWVVAMAAGPGTHLSLFWARIATLAVWAAAALVTVPTAVFG
VEGEVCGVRLCLLRFPSRYWLGAYQLQRVVLAFMVPLGVITTSYLLLLAFLQRRQRRRQD
SRVVARSVRILVASFFLCWFPNHVVTLWGVLVKFDLVPWNSTFYTIQTYVFPVTTCLAHS
NSCLNPVLYCLLRREPRQALAGTFRDLRSRLWPQGGGWVQQVALKQVGRRWVASNPRESR
PSTLLTNLDRGTPG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2255 Download

>pFN21AE2255  5827 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCCACACTCAATACTTCTGCCTCTCCACCCAC
ATTCTTCTGGGCCAATGCCTCCGGAGGCAGTGTGCTGAGTGCTGATGATGCTCCGATGCC
TGTCAAATTCCTAGCCCTGAGGCTCATGGTTGCCCTGGCCTATGGGCTTGTGGGGGCCAT
TGGCTTGCTGGGAAATTTGGCGGTGCTGTGGGTACTGAGTAACTGTGCCCGGAGAGCCCC
TGGCCCACCTTCAGACACCTTCGTCTTCAACCTGGCTCTGGCGGACCTGGGACTGGCACT
CACTCTCCCCTTTTGGGCAGCCGAGTCGGCACTGGACTTTCACTGGCCCTTCGGAGGTGC
CCTCTGCAAGATGGTTCTGACGGCCACTGTCCTCAACGTCTATGCCAGCATCTTCCTCAT
CACAGCGCTGAGCGTTGCTCGCTACTGGGTGGTGGCCATGGCTGCGGGGCCAGGCACCCA
CCTCTCACTCTTCTGGGCCCGAATAGCCACCCTGGCAGTGTGGGCGGCGGCTGCCCTGGT
GACGGTGCCCACAGCTGTCTTCGGGGTGGAGGGTGAGGTGTGTGGTGTGCGCCTTTGCCT
GCTGCGTTTCCCCAGCAGGTACTGGCTGGGGGCCTACCAGCTGCAGAGGGTGGTGCTGGC
TTTCATGGTGCCCTTGGGCGTCATCACCACCAGCTACCTGCTGCTGCTGGCCTTCCTGCA
GCGGCGGCAACGGCGGCGGCAGGACAGCAGGGTCGTGGCCCGCTCTGTCCGCATCCTGGT
GGCTTCCTTCTTCCTCTGCTGGTTTCCCAACCATGTGGTCACTCTCTGGGGTGTCCTGGT
GAAGTTTGACCTGGTGCCCTGGAACAGTACTTTCTATACTATCCAGACGTATGTCTTCCC
TGTCACTACTTGCTTGGCACACAGCAATAGCTGCCTCAACCCTGTGCTGTACTGTCTCCT
GAGGCGGGAGCCCCGGCAGGCTCTGGCAGGCACCTTCAGGGATCTGCGGTCGAGGCTGTG
GCCCCAGGGCGGAGGCTGGGTGCAACAGGTGGCCCTAAAGCAGGTAGGCAGGCGGTGGGT
CGCAAGCAACCCCCGGGAGAGCCGCCCTTCTACCCTGCTCACCAACCTGGACAGAGGGAC
ACCCGGGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT