(Circular) (Six-base) MAPSORT of: pFN21AE2255.seq Check: 6499 from: 1 to: 5827 pFN21AE2255 5827 bp With 182 enzymes: SgfI * March 28, 2012 12:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3637 278 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 3148 3148 Size: 5827 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2214 2959 3890 5274 5542 1632 Size: 582 745 931 1384 268 1917 Fragments arranged by size: 1917 1384 931 745 582 268 AfeI AGC'GCT Cuts at: 2406 2406 Size: 5827 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 4726 5703 1203 Size: 3523 977 1327 Fragments arranged by size: 3523 1327 977 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AlwNI CAG_nnn'CTG Cuts at: 2601 2962 3570 5142 2601 Size: 361 608 1572 3286 Fragments arranged by size: 3286 1572 608 361 ApaI G_GGCC'C Cuts at: 2479 2479 Size: 5827 ApaLI G'TGCA_C Cuts at: 3884 5040 3884 Size: 1156 4671 ApoI r'AATT_y Cuts at: 1493 2105 2173 3136 3362 3514 5623 1493 Size: 612 68 963 226 152 2109 1697 Fragments arranged by size: 2109 1697 963 612 226 152 68 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 3072 3122 3152 1870 Size: 73 1129 50 30 4545 Fragments arranged by size: 4545 1129 73 50 30 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 2453 2524 2647 2833 Size: 325 217 627 549 117 71 123 186 Cuts at: 2833 2969 3148 4603 618 Size: 136 179 1455 1842 Fragments arranged by size: 1842 1455 627 549 325 217 186 179 136 123 117 71 BanII G_rGCy'C Cuts at: 1391 2218 2479 2952 3146 1391 Size: 827 261 473 194 4072 Fragments arranged by size: 4072 827 473 261 194 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2236 2530 2866 928 Size: 971 337 294 336 3889 Fragments arranged by size: 3889 971 337 336 294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2286 2320 4018 4052 1903 Size: 34 50 34 265 34 1698 34 3678 Fragments arranged by size: 3678 1698 265 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2306 2492 4438 Size: 107 122 71 631 605 634 186 1946 Cuts at: 4438 136 Size: 1525 Fragments arranged by size: 1946 1525 634 631 605 186 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 2408 3244 2408 Size: 836 4991 Bme1580I G_kGCm'C Cuts at: 1790 2209 2341 2479 2529 2652 2838 3888 5044 Size: 419 132 138 50 123 186 1050 1156 Cuts at: 5044 1790 Size: 2573 Fragments arranged by size: 2573 1156 1050 419 186 138 132 123 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2434 3558 4516 Size: 416 53 83 399 24 1017 1124 958 Cuts at: 4516 442 Size: 1753 Fragments arranged by size: 1753 1124 1017 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2357 2433 2357 Size: 76 5751 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4487 1239 Size: 3248 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3952 4817 5115 3952 Size: 865 298 4664 Fragments arranged by size: 4664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4490 882 Size: 617 2991 2219 Fragments arranged by size: 2991 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3618 493 Size: 711 2414 2702 Fragments arranged by size: 2702 2414 711 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2658 3634 4016 4651 Size: 53 83 186 936 1125 976 382 635 Cuts at: 4651 275 Size: 1451 Fragments arranged by size: 1451 1125 976 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2060 4260 4658 4932 5079 5490 2060 Size: 2200 398 274 147 411 2397 Fragments arranged by size: 2397 2200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2055 2085 2268 2298 3003 Size: 30 165 30 652 30 183 30 705 Cuts at: 3003 3033 1178 Size: 30 3972 Fragments arranged by size: 3972 705 652 183 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5827 BseYI C'CCAG_C Cuts at: 2167 2591 2605 3015 5030 5526 2167 Size: 424 14 410 2015 496 2468 Fragments arranged by size: 2468 2015 496 424 410 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5254 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2990 3277 4038 5066 1540 Size: 275 186 989 287 761 1028 2301 Fragments arranged by size: 2301 1028 989 761 287 275 186 BsiHKAI G_wGCw'C Cuts at: 3888 3973 5044 3888 Size: 85 1071 4671 Fragments arranged by size: 4671 1071 85 BsmI GAATG_Cn' Cuts at: 3338 3431 3338 Size: 93 5734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2209 2218 2341 2479 2529 2652 2838 Size: 399 419 9 123 138 50 123 186 Cuts at: 2838 2952 3146 3888 3973 5044 1391 Size: 114 194 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 419 399 194 186 138 123 123 114 85 50 9 BspEI T'CCGG_A Cuts at: 2060 5490 2060 Size: 3430 2397 BspHI T'CATG_A Cuts at: 3716 5446 3716 Size: 1730 4097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2586 2685 3031 3182 844 Size: 1742 99 346 151 3489 Fragments arranged by size: 3489 1742 346 151 99 BsrBI CCG'CTC Cuts at: 1394 1823 2742 3714 1394 Size: 429 919 972 3507 Fragments arranged by size: 3507 972 919 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4322 4496 65 Size: 1183 3074 174 1396 Fragments arranged by size: 3074 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4471 4658 1700 Size: 110 2661 187 2869 Fragments arranged by size: 2869 2661 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 3887 4899 3887 Size: 1012 4815 BstAPI GCAn_nnn'nTGC Cuts at: 794 2910 794 Size: 2116 3711 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstXI CCAn_nnnn'nTGG Cuts at: 2794 2794 Size: 5827 BstYI r'GATC_y Cuts at: 1972 2980 3157 3524 3909 3926 5378 5464 5476 Size: 1008 177 367 385 17 1452 86 12 Cuts at: 5476 5822 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1008 385 367 346 177 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2117 2939 2117 Size: 822 5005 BtgI C'CryG_G Cuts at: 513 1065 2436 513 Size: 552 1371 3904 Fragments arranged by size: 3904 1371 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 2074 2502 3343 4137 4157 2074 Size: 428 841 794 20 3744 Fragments arranged by size: 3744 841 794 428 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5792 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2240 2729 4834 809 Size: 507 924 489 2105 1802 Fragments arranged by size: 2105 1802 924 507 489 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2433 3274 Size: 54 1425 50 275 18 531 72 841 Cuts at: 3274 4163 5792 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 841 531 275 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3274 1537 Size: 275 1462 4090 Fragments arranged by size: 4090 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2474 3757 1353 Size: 1121 1283 3423 Fragments arranged by size: 3423 1283 1121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2276 3024 4414 4788 4934 387 Size: 1064 138 687 748 1390 374 146 1280 Fragments arranged by size: 1390 1280 1064 748 687 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 2959 3890 4487 5274 5542 1239 Size: 393 582 745 931 597 787 268 1524 Fragments arranged by size: 1524 931 787 745 597 582 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3038 3038 Size: 5827 EcoO109I rG'GnC_Cy Cuts at: 2610 2610 Size: 5827 EcoRI G'AATT_C Cuts at: 3136 3514 3136 Size: 378 5449 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 2408 4974 1678 Size: 730 2566 2531 Fragments arranged by size: 2566 2531 730 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2262 2294 2972 3004 4468 Size: 32 135 32 690 32 678 32 1464 Cuts at: 4468 4500 4542 4574 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1464 690 678 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3171 3417 669 Size: 988 281 1233 246 3079 Fragments arranged by size: 3079 1233 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KpnI G_GTAC'C Cuts at: 3152 3152 Size: 5827 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4941 5125 1305 Size: 3636 184 2007 Fragments arranged by size: 3636 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 10 Size: 54 1425 343 603 3402 Fragments arranged by size: 3402 1425 603 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2756 3785 4144 4303 518 Size: 648 1590 1029 359 159 2042 Fragments arranged by size: 2042 1590 1029 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2534 2620 2701 3243 3920 5068 5313 1606 Size: 928 86 81 542 677 1148 245 2120 Fragments arranged by size: 2120 1148 928 677 542 245 86 81 NcoI C'CATG_G Cuts at: 513 1065 2436 513 Size: 552 1371 3904 Fragments arranged by size: 3904 1371 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 3185 4730 1132 Size: 97 1956 1545 2229 Fragments arranged by size: 2229 1956 1545 97 PasI CC'CwG_GG Cuts at: 1348 3004 1348 Size: 1656 4171 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5827 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2870 1613 Size: 1257 4570 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PspOMI G'GGCC_C Cuts at: 2475 2475 Size: 5827 PstI C_TGCA'G Cuts at: 830 2625 2700 3179 830 Size: 1795 75 479 3478 Fragments arranged by size: 3478 1795 479 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 PvuII CAG'CTG Cuts at: 2534 2620 2534 Size: 86 5741 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 2848 4075 5787 2848 Size: 1227 1712 2888 Fragments arranged by size: 2888 1712 1227 SexAI A'CCwGG_T Cuts at: 2829 2829 Size: 5827 SfcI C'TryA_G Cuts at: 826 1045 2621 2696 3175 4310 4991 5182 826 Size: 219 1576 75 479 1135 681 191 1471 Fragments arranged by size: 1576 1471 1135 681 479 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 3074 3124 3154 1872 Size: 1202 50 30 4545 Fragments arranged by size: 4545 1202 50 30 SmlI C'TyrA_G Cuts at: 820 1017 1943 3931 4832 5094 820 Size: 197 926 1988 901 262 1553 Fragments arranged by size: 1988 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 3185 1132 Size: 2053 3774 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5827 StyI C'CwwG_G Cuts at: 513 1065 2436 2652 3266 513 Size: 552 1371 216 614 3074 Fragments arranged by size: 3074 1371 614 552 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2050 2416 2471 3007 3854 3871 4024 Size: 1255 470 366 55 536 847 17 153 Cuts at: 4024 4209 4654 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 847 536 470 445 366 185 153 55 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2846 2928 4073 Size: 274 80 33 51 1443 869 82 1145 Cuts at: 4073 5785 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1145 869 274 138 82 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2055 2087 2268 2300 3003 3035 5787 5819 2055 Size: 32 181 32 703 32 2752 32 2063 Fragments arranged by size: 2752 2063 703 181 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5827 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2164 2164 Size: 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 3072 3122 3152 1870 Size: 1202 50 30 4545 Fragments arranged by size: 4545 1202 50 30 XmnI GAAnn'nnTTC Cuts at: 3956 3956 Size: 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3635 276 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI