METTL2B ORF clone pFN21AE2197

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2197

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Clone info.

Species

human

Clone Name

pFN21AE2197 Sequence (with vector) Restriction Map

Product ID

FHC23076

ORF Sequence

DNA sequence (1134 bp)
Protein sequence (378 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2197

Symbol

METTL2B

Alias : METL, METTL2, METTL2A, PSENIP1

Description

methyltransferase like 2B

Original Clone ID

cs03297

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1134 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 378 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5803.2 2.9e-170 99.7 METTL2B CCDS45752.1 1.7e-167 98.7 METTL2A CCDS2242.2 9.7e-84 55.4 METTL8 CCDS82532.1 1.4e-49 49.2 METTL8 CCDS82531.1 2.1e-42 47.9 METTL8 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013217 185 287 PF08242 Methyltransferase type 12
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 249 VPKGSLDIIILIFVLSAIVPDKM 271 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060866 378 607846 99.7 100.0 NP_079046 407 609525 55.4 93.7 NP_001308083 407 609525 55.4 93.7 NP_001308085 407 609525 55.4 93.7 NP_001308084 407 609525 55.4 93.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2197 Download

>KIEE2197  1134 bp
ATGGCCGGCTCCTACCCTGAAGGTGCACCTGCAATCCTCGCCGATAAGAGGCAGCAGTTC
GGAAGCCGGTTCCTGAGCGATCCGGCGCGCGTCTTCCACCACAATGCCTGGGACAATGTG
GAGTGGTCGGAAGAGCAAGCCGCGGCGGCGGAGAGAAAAGTCCAGGAGAACAGTATCCAG
CGGGTGTGCCAGGAGAAACAAGTTGATTATGAGATCAATGCCCACAAATACTGGAATGAC
TTCTACAAAATCCACGAAAATGGGTTTTTCAAGGATAGACATTGGCTTTTTACCGAATTC
CCTGAGCTGGCACCTAGCCAAAATCAAAATCATTTGAAGGATTGGTTCTTGGAGAACAAG
AGTGAAGTATGTGAATGTAGAAACAATGAGGATGGACCTGGTTTAATAATGGAAGAACAG
CACAAGTGTTCTTCGAAGAGCCTTGAACATAAAACACAGACACCTCCTGTGGAGGAGAAT
GTAACTCAGAAAATTAGTGACCTGGAAATTTGTGCTGATGAGTTTCCTGGATCCTCAGCC
ACCTACCGAATACTGGAGGTTGGCTGTGGTGTGGGAAACACAGTCTTTCCAATTTTACAA
ACGAACAATGACCCAGGACTCTTTGTTTATTGCTGTGATTTTTCTTCCACAGCTATAGAA
CTGGTCCAGACAAATTCAGAATATGATCCTTCTCGGTGTTTTGCCTTTGTTCACGACCTG
TGTGATGAAGAGAAGAGTTACCCAGTGCCCAAGGGCAGTCTTGATATTATCATTCTCATA
TTTGTTCTTTCAGCAATTGTTCCAGACAAGATGCAGAAGGCTATCAACAGGCTGAGCAGG
CTTCTGAAACCTGGGGGGATGGTACTTCTGCGAGATTACGGCCGCTATGACATGGCTCAG
CTTCGGTTTAAAAAAGGTCAGTGTCTATCTGGAAATTTCTATGTGAGAGGTGATGGAACC
AGAGTTTACTTCTTCACACAAGAGGAACTGGACACGCTTTTCACCACTGCTGGACTGGAA
AAAGTTCAGAACCTGGTGGACCGCCGACTGCAGGTGAACCGAGGGAAGCAACTGACAATG
TACCGGGTTTGGATTCAGTGCAAATACTGCAAGCCCCTTCTGTCCAGCACCAGC

ORF Protein

Cloned ORF protein sequence for pFN21AE2197 Download

>KIEE2197  378 aa
MAGSYPEGAPAILADKRQQFGSRFLSDPARVFHHNAWDNVEWSEEQAAAAERKVQENSIQ
RVCQEKQVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENK
SEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSA
TYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDL
CDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQ
LRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTM
YRVWIQCKYCKPLLSSTS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2197 Download

>pFN21AE2197  5839 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCGGCTCCTACCCTGAAGGTGCACCTGCAAT
CCTCGCCGATAAGAGGCAGCAGTTCGGAAGCCGGTTCCTGAGCGATCCGGCGCGCGTCTT
CCACCACAATGCCTGGGACAATGTGGAGTGGTCGGAAGAGCAAGCCGCGGCGGCGGAGAG
AAAAGTCCAGGAGAACAGTATCCAGCGGGTGTGCCAGGAGAAACAAGTTGATTATGAGAT
CAATGCCCACAAATACTGGAATGACTTCTACAAAATCCACGAAAATGGGTTTTTCAAGGA
TAGACATTGGCTTTTTACCGAATTCCCTGAGCTGGCACCTAGCCAAAATCAAAATCATTT
GAAGGATTGGTTCTTGGAGAACAAGAGTGAAGTATGTGAATGTAGAAACAATGAGGATGG
ACCTGGTTTAATAATGGAAGAACAGCACAAGTGTTCTTCGAAGAGCCTTGAACATAAAAC
ACAGACACCTCCTGTGGAGGAGAATGTAACTCAGAAAATTAGTGACCTGGAAATTTGTGC
TGATGAGTTTCCTGGATCCTCAGCCACCTACCGAATACTGGAGGTTGGCTGTGGTGTGGG
AAACACAGTCTTTCCAATTTTACAAACGAACAATGACCCAGGACTCTTTGTTTATTGCTG
TGATTTTTCTTCCACAGCTATAGAACTGGTCCAGACAAATTCAGAATATGATCCTTCTCG
GTGTTTTGCCTTTGTTCACGACCTGTGTGATGAAGAGAAGAGTTACCCAGTGCCCAAGGG
CAGTCTTGATATTATCATTCTCATATTTGTTCTTTCAGCAATTGTTCCAGACAAGATGCA
GAAGGCTATCAACAGGCTGAGCAGGCTTCTGAAACCTGGGGGGATGGTACTTCTGCGAGA
TTACGGCCGCTATGACATGGCTCAGCTTCGGTTTAAAAAAGGTCAGTGTCTATCTGGAAA
TTTCTATGTGAGAGGTGATGGAACCAGAGTTTACTTCTTCACACAAGAGGAACTGGACAC
GCTTTTCACCACTGCTGGACTGGAAAAAGTTCAGAACCTGGTGGACCGCCGACTGCAGGT
GAACCGAGGGAAGCAACTGACAATGTACCGGGTTTGGATTCAGTGCAAATACTGCAAGCC
CCTTCTGTCCAGCACCAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT