CRHR2 ORF clone pFN21AE1885

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Halotag ORF Clone pFN21AE1885

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Clone info.

Species

human

Clone Name

pFN21AE1885 Sequence (with vector) Restriction Map

Product ID

FHC22891

ORF Sequence

DNA sequence (1233 bp)
Protein sequence (411 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE1885

Symbol

CRHR2

Alias : CRF-RB, CRF2, CRFR2, HM-CRF

Description

corticotropin releasing hormone receptor 2, transcript variant 1

Original Clone ID

cs02785

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1233 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 411 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5429.1 1.3e-179 100.0 CRHR2 CCDS56477.1 6e-165 99.0 CRHR2 CCDS56478.1 1.8e-164 100.0 CRHR2 CCDS75576.1 5.4e-159 100.0 CRHR2 CCDS42350.1 6e-126 74.5 CRHR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003053 29 37 PR01281 GPCR IPR003051 52 63 PR01279 GPCR IPR003053 53 61 PR01281 GPCR IPR003051 65 82 PR01279 GPCR IPR003051 86 102 PR01279 GPCR IPR003053 97 106 PR01281 GPCR IPR003051 107 122 PR01279 GPCR IPR000832 118 142 PR00249 GPCR IPR003051 124 142 PR01279 GPCR IPR000832 150 174 PR00249 GPCR IPR003053 168 183 PR01281 GPCR IPR000832 186 209 PR00249 GPCR IPR003051 211 223 PR01279 GPCR IPR000832 224 249 PR00249 GPCR IPR003051 255 270 PR01279 GPCR IPR003053 258 265 PR01281 GPCR IPR000832 266 291 PR00249 GPCR IPR003051 295 304 PR01279 GPCR IPR000832 308 328 PR00249 GPCR IPR003051 326 347 PR01279 GPCR IPR000832 341 362 PR00249 GPCR IPR003051 389 407 PR01279 GPCR HMMPfam IPR001879 38 99 PF02793 GPCR IPR000832 117 355 PF00002 GPCR HMMSmart IPR001879 36 107 SM00008 GPCR ProfileScan IPR001879 13 102 PS50227 GPCR IPR017981 116 363 PS50261 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 117 LVVNYLGHCVSVAALVAAFLLFL 139 PRIMARY 23 2 154 NLITTFILRNVMWFLLQLVDHEV 176 SECONDARY 23 3 189 TIFNYFVVTNFFWMFVEGCYLHT 211 SECONDARY 23 4 225 CLFLFIGWCIPFPIIVAWAIGKL 247 PRIMARY 23 5 271 PIILVLLINFVFLFNIVRILMTK 293 PRIMARY 23 6 309 VKATLVLLPLLGITYMLFFVNPG 331 SECONDARY 23 7 339 MFIYFNSFLQSFQGFFVSVFYCF 361 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001874 411 602034 100.0 100.0 NP_001189411 410 602034 99.8 100.0 NP_001189410 397 602034 99.0 93.2 XP_016867241 397 602034 99.0 93.2 NP_001189404 438 602034 100.0 91.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1885 Download

>KIEE1885  1233 bp
ATGGACGCGGCACTGCTCCACAGCCTGCTGGAGGCCAACTGCAGCCTGGCGCTGGCTGAA
GAGCTGCTCTTGGACGGCTGGGGGCCACCCCTGGACCCCGAGGGTCCCTACTCCTACTGC
AACACGACCTTGGACCAGATCGGAACGTGCTGGCCCCGCAGCGCTGCCGGAGCCCTCGTG
GAGAGGCCGTGCCCCGAGTACTTCAACGGCGTCAAGTACAACACGACCCGGAATGCCTAT
CGAGAATGCTTGGAGAATGGGACGTGGGCCTCAAAGATCAACTACTCACAGTGTGAGCCC
ATTTTGGATGACAAGCAGAGGAAGTATGACCTGCACTACCGCATCGCCCTTGTCGTCAAC
TACCTGGGCCACTGCGTATCTGTGGCAGCCCTGGTGGCCGCCTTCCTGCTTTTCCTGGCC
CTGCGGAGCATTCGCTGTCTGCGGAATGTGATTCACTGGAACCTCATCACCACCTTTATC
CTGCGAAATGTCATGTGGTTCCTGCTGCAGCTCGTTGACCATGAAGTGCACGAGAGCAAT
GAGGTCTGGTGCCGCTGCATCACCACCATCTTCAACTACTTCGTGGTGACCAACTTCTTC
TGGATGTTTGTGGAAGGCTGCTACCTGCACACGGCCATTGTCATGACCTACTCCACTGAG
CGCCTGCGCAAGTGCCTCTTCCTCTTCATCGGATGGTGCATCCCCTTCCCCATCATCGTC
GCCTGGGCCATCGGCAAGCTCTACTATGAGAATGAACAGTGCTGGTTTGGCAAGGAGCCT
GGCGACCTGGTGGACTACATCTACCAAGGCCCCATCATTCTCGTGCTCCTGATCAATTTC
GTATTTCTGTTCAACATCGTCAGGATCCTAATGACAAAGTTACGCGCGTCCACCACATCC
GAGACAATCCAGTACAGGAAGGCAGTGAAGGCCACCCTGGTGCTCCTGCCCCTCCTGGGC
ATCACCTACATGCTCTTCTTCGTCAATCCCGGGGAGGACGACCTGTCACAGATCATGTTC
ATCTATTTCAACTCCTTCCTGCAGTCGTTCCAGGGTTTCTTCGTGTCTGTCTTCTACTGC
TTCTTCAATGGAGAGGTGCGCTCAGCCGTGAGGAAGAGGTGGCACCGCTGGCAGGACCAT
CACTCCCTTCGAGTCCCCATGGCCCGGGCCATGTCCATCCCTACATCACCCACACGGATC
AGCTTCCACAGCATCAAGCAGACGGCCGCTGTG

ORF Protein

Cloned ORF protein sequence for pFN21AE1885 Download

>KIEE1885  411 aa
MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALV
ERPCPEYFNGVKYNTTRNAYRECLENGTWASKINYSQCEPILDDKQRKYDLHYRIALVVN
YLGHCVSVAALVAAFLLFLALRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESN
EVWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIV
AWAIGKLYYENEQCWFGKEPGDLVDYIYQGPIILVLLINFVFLFNIVRILMTKLRASTTS
ETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQGFFVSVFYC
FFNGEVRSAVRKRWHRWQDHHSLRVPMARAMSIPTSPTRISFHSIKQTAAV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1885 Download

>pFN21AE1885  5938 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACGCGGCACTGCTCCACAGCCTGCTGGAGGC
CAACTGCAGCCTGGCGCTGGCTGAAGAGCTGCTCTTGGACGGCTGGGGGCCACCCCTGGA
CCCCGAGGGTCCCTACTCCTACTGCAACACGACCTTGGACCAGATCGGAACGTGCTGGCC
CCGCAGCGCTGCCGGAGCCCTCGTGGAGAGGCCGTGCCCCGAGTACTTCAACGGCGTCAA
GTACAACACGACCCGGAATGCCTATCGAGAATGCTTGGAGAATGGGACGTGGGCCTCAAA
GATCAACTACTCACAGTGTGAGCCCATTTTGGATGACAAGCAGAGGAAGTATGACCTGCA
CTACCGCATCGCCCTTGTCGTCAACTACCTGGGCCACTGCGTATCTGTGGCAGCCCTGGT
GGCCGCCTTCCTGCTTTTCCTGGCCCTGCGGAGCATTCGCTGTCTGCGGAATGTGATTCA
CTGGAACCTCATCACCACCTTTATCCTGCGAAATGTCATGTGGTTCCTGCTGCAGCTCGT
TGACCATGAAGTGCACGAGAGCAATGAGGTCTGGTGCCGCTGCATCACCACCATCTTCAA
CTACTTCGTGGTGACCAACTTCTTCTGGATGTTTGTGGAAGGCTGCTACCTGCACACGGC
CATTGTCATGACCTACTCCACTGAGCGCCTGCGCAAGTGCCTCTTCCTCTTCATCGGATG
GTGCATCCCCTTCCCCATCATCGTCGCCTGGGCCATCGGCAAGCTCTACTATGAGAATGA
ACAGTGCTGGTTTGGCAAGGAGCCTGGCGACCTGGTGGACTACATCTACCAAGGCCCCAT
CATTCTCGTGCTCCTGATCAATTTCGTATTTCTGTTCAACATCGTCAGGATCCTAATGAC
AAAGTTACGCGCGTCCACCACATCCGAGACAATCCAGTACAGGAAGGCAGTGAAGGCCAC
CCTGGTGCTCCTGCCCCTCCTGGGCATCACCTACATGCTCTTCTTCGTCAATCCCGGGGA
GGACGACCTGTCACAGATCATGTTCATCTATTTCAACTCCTTCCTGCAGTCGTTCCAGGG
TTTCTTCGTGTCTGTCTTCTACTGCTTCTTCAATGGAGAGGTGCGCTCAGCCGTGAGGAA
GAGGTGGCACCGCTGGCAGGACCATCACTCCCTTCGAGTCCCCATGGCCCGGGCCATGTC
CATCCCTACATCACCCACACGGATCAGCTTCCACAGCATCAAGCAGACGGCCGCTGTGGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT