(Circular) (Six-base) MAPSORT of: pFN21AE1885.seq Check: 6647 from: 1 to: 5938 pFN21AE1885 5938 bp With 182 enzymes: SgfI * March 28, 2012 11:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3281 1937 Size: 1344 4594 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2082 4001 5385 5653 1632 Size: 450 1919 1384 268 1917 Fragments arranged by size: 1919 1917 1384 450 268 AfeI AGC'GCT Cuts at: 2167 2167 Size: 5938 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 4837 5814 1203 Size: 3634 977 1327 Fragments arranged by size: 3634 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 3007 4667 3007 Size: 1660 4278 AleI CACnn'nnGTG Cuts at: 1166 2942 1166 Size: 1776 4162 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 3681 5253 3681 Size: 1572 4366 ApaLI G'TGCA_C Cuts at: 2531 3995 5151 2531 Size: 1464 1156 3318 Fragments arranged by size: 3318 1464 1156 ApoI r'AATT_y Cuts at: 1493 3247 3473 3625 5734 1493 Size: 1754 226 152 2109 1697 Fragments arranged by size: 2109 1754 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 2102 2198 2993 3168 3263 1870 Size: 73 159 96 795 175 95 4545 Fragments arranged by size: 4545 795 175 159 96 95 73 BamHI G'GATC_C Cuts at: 2868 3268 3635 2868 Size: 400 367 5171 Fragments arranged by size: 5171 400 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2553 3126 3259 4714 618 Size: 325 217 627 766 573 133 1455 1842 Fragments arranged by size: 1842 1455 766 627 573 325 217 133 BanII G_rGCy'C Cuts at: 1391 2179 2304 3257 1391 Size: 788 125 953 4072 Fragments arranged by size: 4072 953 788 125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3067 928 Size: 971 1168 3799 Fragments arranged by size: 3799 1168 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3830 5040 3830 Size: 1210 4728 BclI T'GATC_A Cuts at: 1548 2835 1548 Size: 1287 4651 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2399 4549 136 Size: 107 122 71 631 605 727 2150 1525 Fragments arranged by size: 2150 1525 727 631 605 122 107 71 BglII A'GATC_T Cuts at: 5933 5933 Size: 5938 BlpI GC'TnA_GC Cuts at: 3106 3355 3106 Size: 249 5689 Bme1580I G_kGCm'C Cuts at: 1790 2198 2535 3999 5155 1790 Size: 408 337 1464 1156 2573 Fragments arranged by size: 2573 1464 1156 408 337 BmgBI CAC'GTC Cuts at: 1318 2268 1318 Size: 950 4988 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3669 4627 442 Size: 416 53 83 399 24 2252 958 1753 Fragments arranged by size: 2252 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3121 3121 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 4598 1239 Size: 815 2544 2579 Fragments arranged by size: 2579 2544 815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4063 4928 5226 4063 Size: 865 298 4775 Fragments arranged by size: 4775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4601 882 Size: 617 3102 2219 Fragments arranged by size: 3102 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2214 3745 4127 4762 Size: 53 83 186 936 681 1531 382 635 Cuts at: 4762 275 Size: 1451 Fragments arranged by size: 1531 1451 936 681 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4371 4769 5043 5190 5601 4371 Size: 398 274 147 411 4708 Fragments arranged by size: 4708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2166 2177 2196 2207 3131 Size: 30 165 30 763 11 19 11 924 Cuts at: 3131 3161 1178 Size: 30 3955 Fragments arranged by size: 3955 924 763 165 30 30 30 19 11 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5938 BseYI C'CCAG_C Cuts at: 2082 5141 5637 2082 Size: 3059 496 2383 Fragments arranged by size: 3059 2383 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2321 2615 1163 Size: 573 585 294 4486 Fragments arranged by size: 4486 585 573 294 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3231 3388 4149 5177 1540 Size: 275 186 1230 157 761 1028 2301 Fragments arranged by size: 2301 1230 1028 761 275 186 157 BsiHKAI G_wGCw'C Cuts at: 2535 2832 2949 3999 4084 5155 2535 Size: 297 117 1050 85 1071 3318 Fragments arranged by size: 3318 1071 1050 297 117 85 BsmI GAATG_Cn' Cuts at: 2242 2255 2433 3449 3542 2242 Size: 13 178 1016 93 4638 Fragments arranged by size: 4638 1016 178 93 13 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2179 2198 2304 2535 2832 2949 3257 Size: 399 389 19 106 231 297 117 308 Cuts at: 3257 3999 4084 5155 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 389 308 297 231 117 106 85 19 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 2646 3827 5557 2646 Size: 1181 1730 3027 Fragments arranged by size: 3027 1730 1181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2343 2637 3293 844 Size: 1499 294 656 3489 Fragments arranged by size: 3489 1499 656 294 BsrBI CCG'CTC Cuts at: 1394 1823 3825 1394 Size: 429 2002 3507 Fragments arranged by size: 3507 2002 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2548 4433 4607 65 Size: 1183 1300 1885 174 1396 Fragments arranged by size: 1885 1396 1300 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4582 4769 1700 Size: 110 2772 187 2869 Fragments arranged by size: 2869 2772 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5938 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 2180 2534 2825 3998 5010 2180 Size: 354 291 1173 1012 3108 Fragments arranged by size: 3108 1173 1012 354 291 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 2590 1184 Size: 1406 4532 BstYI r'GATC_y Cuts at: 1972 2868 3268 3635 4020 4037 5489 5575 5587 Size: 896 400 367 385 17 1452 86 12 Cuts at: 5587 5933 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 896 400 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3162 513 Size: 552 939 1158 3289 Fragments arranged by size: 3289 1158 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2332 487 Size: 683 56 1106 4093 Fragments arranged by size: 4093 1106 683 56 BtsI GCAGTG_nn' Cuts at: 2015 2374 2934 3454 4248 4268 2015 Size: 359 560 520 794 20 3685 Fragments arranged by size: 3685 794 560 520 359 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DraIII CAC_nnn'GTG Cuts at: 2297 2297 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2400 2637 3228 Size: 54 1425 50 275 18 570 237 591 Cuts at: 3228 3385 4274 5903 8 Size: 157 889 1629 43 Fragments arranged by size: 1629 1425 889 591 570 275 237 157 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3228 3385 1537 Size: 275 1416 157 4090 Fragments arranged by size: 4090 1416 275 157 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2687 2693 2984 3113 3868 1353 Size: 705 629 6 291 129 755 3423 Fragments arranged by size: 3423 755 705 629 291 129 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4525 4899 5045 387 Size: 1064 138 2936 374 146 1280 Fragments arranged by size: 2936 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2082 4001 4598 5385 5653 1239 Size: 393 422 28 1919 597 787 268 1524 Fragments arranged by size: 1919 1524 787 597 422 393 268 28 EcoNI CCTnn'n_nnAGG Cuts at: 2033 2033 Size: 5938 EcoO109I rG'GnC_Cy Cuts at: 2108 2813 2108 Size: 705 5233 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 5938 FspI TGC'GCA Cuts at: 2672 4444 2672 Size: 1772 4166 HaeII r_GCGC'y Cuts at: 1678 2057 2169 2668 5085 1678 Size: 379 112 499 2417 2531 Fragments arranged by size: 2531 2417 499 379 112 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2688 2720 2993 3025 3131 Size: 32 135 32 1116 32 273 32 106 Cuts at: 3131 3163 4579 4611 4653 4685 1373 Size: 32 1416 32 42 32 2626 Fragments arranged by size: 2626 1416 1116 273 135 106 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 2521 3282 3528 669 Size: 988 281 424 159 761 246 3079 Fragments arranged by size: 3079 988 761 424 281 246 159 HindIII A'AGCT_T Cuts at: 748 748 Size: 5938 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 3537 3537 Size: 5938 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5052 5236 1305 Size: 3747 184 2007 Fragments arranged by size: 3747 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4116 Fragments arranged by size: 4116 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 2825 2942 2972 3896 4255 4414 Size: 648 1479 180 117 30 924 359 159 Cuts at: 4414 518 Size: 2042 Fragments arranged by size: 2042 1479 924 648 359 180 159 117 30 MspA1I CmG'CkG Cuts at: 1606 2559 3132 3233 3354 4031 5179 5424 1606 Size: 953 573 101 121 677 1148 245 2120 Fragments arranged by size: 2120 1148 953 677 573 245 121 101 NcoI C'CATG_G Cuts at: 513 1065 2004 3162 513 Size: 552 939 1158 3289 Fragments arranged by size: 3289 1158 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2977 3296 4841 1132 Size: 97 1748 319 1545 2229 Fragments arranged by size: 2229 1748 1545 319 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5938 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5938 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2108 2108 Size: 5938 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PstI C_TGCA'G Cuts at: 830 2048 2514 3048 3290 830 Size: 1218 466 534 242 3478 Fragments arranged by size: 3478 1218 534 466 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SanDI GG'GwC_CC Cuts at: 2108 2108 Size: 5938 SapI GCTCTTCn'nnn_ Cuts at: 2058 2984 2058 Size: 926 5012 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 2204 4186 5898 2204 Size: 1982 1712 2244 Fragments arranged by size: 2244 1982 1712 SexAI A'CCwGG_T Cuts at: 2790 2790 Size: 5938 SfcI C'TryA_G Cuts at: 826 1045 2044 2510 3044 3286 4421 5102 5293 Size: 219 999 466 534 242 1135 681 191 Cuts at: 5293 826 Size: 1471 Fragments arranged by size: 1471 1135 999 681 534 466 242 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 2995 3170 3265 1872 Size: 1123 175 95 4545 Fragments arranged by size: 4545 1123 175 95 SmlI C'TyrA_G Cuts at: 820 1017 1943 4042 4943 5205 820 Size: 197 926 2099 901 262 1553 Fragments arranged by size: 2099 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SrfI GCCC'GGGC Cuts at: 3170 3170 Size: 5938 SspI AAT'ATT Cuts at: 5 52 3862 5 Size: 47 3810 2081 Fragments arranged by size: 3810 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5938 StyI C'CwwG_G Cuts at: 513 1065 2004 2133 2809 3162 3377 513 Size: 552 939 129 676 353 215 3074 Fragments arranged by size: 3074 939 676 552 353 215 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3208 3965 3982 4135 4320 4765 325 Size: 1255 1628 757 17 153 185 445 1498 Fragments arranged by size: 1628 1498 1255 757 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 2220 2916 Size: 274 80 33 51 1443 225 18 696 Cuts at: 2916 4184 5896 96 Size: 1268 1712 138 Fragments arranged by size: 1712 1443 1268 696 274 225 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2166 2177 2198 2209 5898 5930 2166 Size: 11 21 11 3689 32 2174 Fragments arranged by size: 3689 2174 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5938 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2047 2047 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 2993 3168 3263 1870 Size: 1123 175 95 4545 Fragments arranged by size: 4545 1123 175 95 XmnI GAAnn'nnTTC Cuts at: 4067 4067 Size: 5938 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SwaI