GFRA1 ORF clone pFN21AE1556

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1556

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AE1556 Sequence (with vector) Restriction Map

Product ID

FHC08766

ORF Sequence

DNA sequence (1380 bp)
Protein sequence (460 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590245]

Gene

KIEE1556

Symbol

GFRA1

Alias : GDNFR, GDNFRA, GFR-ALPHA-1, GFRalpha-1, RET1L, RETL1, TRNR1

Description

GDNF family receptor alpha 1, transcript variant 2

Original Clone ID

oc10079

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1380 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 460 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7593.1 1.9e-187 100.0 GFRA1 CCDS44481.1 3.2e-186 98.9 GFRA1 CCDS47816.1 1.5e-82 49.8 GFRA2 CCDS55208.1 9.3e-63 49.4 GFRA2 CCDS55207.1 8.8e-58 48.0 GFRA2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003503 58 70 PR01317 Glial cell line-derived neurotrophic factor receptor IPR003503 80 86 PR01317 Glial cell line-derived neurotrophic factor receptor IPR003438 87 107 PR01316 Glial cell line-derived neurotrophic factor receptor IPR003438 181 198 PR01316 Glial cell line-derived neurotrophic factor receptor IPR003438 216 228 PR01316 Glial cell line-derived neurotrophic factor receptor IPR003438 250 258 PR01316 Glial cell line-derived neurotrophic factor receptor IPR003438 280 297 PR01316 Glial cell line-derived neurotrophic factor receptor IPR003438 308 321 PR01316 Glial cell line-derived neurotrophic factor receptor IPR003503 349 370 PR01317 Glial cell line-derived neurotrophic factor receptor HMMPfam IPR016017 29 109 PF02351 GDNF/GAS1 IPR016017 149 228 PF02351 GDNF/GAS1 IPR016017 238 332 PF02351 GDNF/GAS1 HMMSmart IPR016017 29 111 SM00907 GDNF/GAS1 IPR016017 149 228 SM00907 GDNF/GAS1 IPR016017 238 332 SM00907 GDNF/GAS1
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MFLATLYFALPLLDLLLSAEVSG 23 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_665736 460 601496 100.0 100.0 NP_001138925 460 601496 100.0 100.0 NP_005255 465 601496 98.9 100.0 XP_005269753 465 601496 98.9 100.0 XP_011537936 465 601496 98.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1556 Download

>KIEE1556  1380 bp
ATGTTCCTGGCGACCCTGTACTTCGCGCTGCCGCTCTTGGACTTGCTCCTGTCGGCCGAA
GTGAGCGGCGGAGACCGCCTGGATTGCGTGAAAGCCAGTGATCAGTGCCTGAAGGAGCAG
AGCTGCAGCACCAAGTACCGCACGCTAAGGCAGTGCGTGGCGGGCAAGGAGACCAACTTC
AGCCTGGCATCCGGCCTGGAGGCCAAGGATGAGTGCCGCAGCGCCATGGAGGCCCTGAAG
CAGAAGTCGCTCTACAACTGCCGCTGCAAGCGGGGTATGAAGAAGGAGAAGAACTGCCTG
CGCATTTACTGGAGCATGTACCAGAGCCTGCAGGGAAATGATCTGCTGGAGGATTCCCCA
TATGAACCAGTTAACAGCAGATTGTCAGATATATTCCGGGTGGTCCCATTCATATCAGTG
GAGCACATTCCCAAAGGGAACAACTGCCTGGATGCAGCGAAGGCCTGCAACCTCGACGAC
ATTTGCAAGAAGTACAGGTCGGCGTACATCACCCCGTGCACCACCAGCGTGTCCAATGAT
GTCTGCAACCGCCGCAAGTGCCACAAGGCCCTCCGGCAGTTCTTTGACAAGGTCCCGGCC
AAGCACAGCTACGGAATGCTCTTCTGCTCCTGCCGGGACATCGCCTGCACAGAGCGGAGG
CGACAGACCATCGTGCCTGTGTGCTCCTATGAAGAGAGGGAGAAGCCCAACTGTTTGAAT
TTGCAGGACTCCTGCAAGACGAATTACATCTGCAGATCTCGCCTTGCGGATTTTTTTACC
AACTGCCAGCCAGAGTCAAGGTCTGTCAGCAGCTGTCTAAAGGAAAACTACGCTGACTGC
CTCCTCGCCTACTCGGGGCTTATTGGCACAGTCATGACCCCCAACTACATAGACTCCAGT
AGCCTCAGTGTGGCCCCATGGTGTGACTGCAGCAACAGTGGGAACGACCTAGAAGAGTGC
TTGAAATTTTTGAATTTCTTCAAGGACAATACATGTCTTAAAAATGCAATTCAAGCCTTT
GGCAATGGCTCCGATGTGACCGTGTGGCAGCCAGCCTTCCCAGTACAGACCACCACTGCC
ACTACCACCACTGCCCTCCGGGTTAAGAACAAGCCCCTGGGGCCAGCAGGGTCTGAGAAT
GAAATTCCCACTCATGTTTTGCCACCGTGTGCAAATTTACAGGCACAGAAGCTGAAATCC
AATGTGTCGGGCAATACACACCTCTGTATTTCCAATGGTAATTATGAAAAAGAAGGTCTC
GGTGCTTCCAGCCACATAACCACAAAATCAATGGCTGCTCCTCCAAGCTGTGGTCTGAGC
CCACTGCTGGTCCTGGTGGTAACCGCTCTGTCCACCCTATTATCTTTAACAGAAACATCA

ORF Protein

Cloned ORF protein sequence for pFN21AE1556 Download

>KIEE1556  460 aa
MFLATLYFALPLLDLLLSAEVSGGDRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNF
SLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSP
YEPVNSRLSDIFRVVPFISVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSND
VCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLN
LQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSS
SLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTA
TTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGL
GASSHITTKSMAAPPSCGLSPLLVLVVTALSTLLSLTETS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1556 Download

>pFN21AE1556  6085 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTCCTGGCGACCCTGTACTTCGCGCTGCCGCT
CTTGGACTTGCTCCTGTCGGCCGAAGTGAGCGGCGGAGACCGCCTGGATTGCGTGAAAGC
CAGTGATCAGTGCCTGAAGGAGCAGAGCTGCAGCACCAAGTACCGCACGCTAAGGCAGTG
CGTGGCGGGCAAGGAGACCAACTTCAGCCTGGCATCCGGCCTGGAGGCCAAGGATGAGTG
CCGCAGCGCCATGGAGGCCCTGAAGCAGAAGTCGCTCTACAACTGCCGCTGCAAGCGGGG
TATGAAGAAGGAGAAGAACTGCCTGCGCATTTACTGGAGCATGTACCAGAGCCTGCAGGG
AAATGATCTGCTGGAGGATTCCCCATATGAACCAGTTAACAGCAGATTGTCAGATATATT
CCGGGTGGTCCCATTCATATCAGTGGAGCACATTCCCAAAGGGAACAACTGCCTGGATGC
AGCGAAGGCCTGCAACCTCGACGACATTTGCAAGAAGTACAGGTCGGCGTACATCACCCC
GTGCACCACCAGCGTGTCCAATGATGTCTGCAACCGCCGCAAGTGCCACAAGGCCCTCCG
GCAGTTCTTTGACAAGGTCCCGGCCAAGCACAGCTACGGAATGCTCTTCTGCTCCTGCCG
GGACATCGCCTGCACAGAGCGGAGGCGACAGACCATCGTGCCTGTGTGCTCCTATGAAGA
GAGGGAGAAGCCCAACTGTTTGAATTTGCAGGACTCCTGCAAGACGAATTACATCTGCAG
ATCTCGCCTTGCGGATTTTTTTACCAACTGCCAGCCAGAGTCAAGGTCTGTCAGCAGCTG
TCTAAAGGAAAACTACGCTGACTGCCTCCTCGCCTACTCGGGGCTTATTGGCACAGTCAT
GACCCCCAACTACATAGACTCCAGTAGCCTCAGTGTGGCCCCATGGTGTGACTGCAGCAA
CAGTGGGAACGACCTAGAAGAGTGCTTGAAATTTTTGAATTTCTTCAAGGACAATACATG
TCTTAAAAATGCAATTCAAGCCTTTGGCAATGGCTCCGATGTGACCGTGTGGCAGCCAGC
CTTCCCAGTACAGACCACCACTGCCACTACCACCACTGCCCTCCGGGTTAAGAACAAGCC
CCTGGGGCCAGCAGGGTCTGAGAATGAAATTCCCACTCATGTTTTGCCACCGTGTGCAAA
TTTACAGGCACAGAAGCTGAAATCCAATGTGTCGGGCAATACACACCTCTGTATTTCCAA
TGGTAATTATGAAAAAGAAGGTCTCGGTGCTTCCAGCCACATAACCACAAAATCAATGGC
TGCTCCTCCAAGCTGTGGTCTGAGCCCACTGCTGGTCCTGGTGGTAACCGCTCTGTCCAC
CCTATTATCTTTAACAGAAACATCAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT