BFAR ORF clone pFN21AE1544

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Halotag ORF Clone pFN21AE1544

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Clone info.

Species

human

Clone Name

pFN21AE1544 Sequence (with vector) Restriction Map

Product ID

FHC09824

ORF Sequence

DNA sequence (1350 bp)
Protein sequence (450 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590367]

Gene

KIEE1544

Symbol

BFAR

Alias : BAR, RNF47

Description

bifunctional apoptosis regulator

Original Clone ID

oc10067

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1350 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 450 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10554.1 1.9e-154 100.0 BFAR CCDS81947.1 1.6e-78 72.2 BFAR The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018957 34 73 PF00097 Zinc finger IPR021129 181 247 PF00536 Sterile alpha motif HMMSmart IPR001841 34 73 SM00184 Zinc finger IPR001660 179 249 SM00454 Sterile alpha motif domain ProfileScan IPR001841 34 74 PS50089 Zinc finger IPR001660 182 249 PS50105 Sterile alpha motif domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 142 GGFFSGVLTALTGVAVVLLVYH 163 PRIMARY 22 2 359 SRFLIINAMLLSVLELFSFWRIW 381 PRIMARY 23 3 401 STQGLFVAMFWPLIPQFVCNCLF 423 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1544 Download

>KIEE1544  1350 bp
ATGGAGGAACCTCAGAAAAGCTATGTGAACACAATGGACCTTGAGAGAGATGAACCTCTC
AAAAGCACCGGCCCTCAGATTTCTGTTAGTGAATTTTCTTGCCACTGCTGCTACGACATC
CTGGTTAACCCCACCACCTTGAACTGTGGGCACAGCTTCTGCCGTCACTGCCTTGCTTTA
TGGTGGGCATCTTCAAAGAAAACAGAATGTCCAGAATGCAGAGAAAAATGGGAAGGTTTC
CCCAAAGTCAGTATTCTCCTCAGGGATGCCATTGAAAAGTTATTTCCTGATGCCATTAGA
CTGAGATTTGAAGACATTCAGCAGAATAATGACATAGTCCAAAGTCTTGCAGCCTTTCAG
AAATATGGGAATGATCAGATTCCTTTAGCTCCTAACACAGGCCGAGCGAATCAGCAGATG
GGAGGGGGATTCTTTTCCGGTGTGCTCACAGCTTTAACTGGAGTGGCAGTGGTCCTGCTC
GTCTATCACTGGAGCAGCAGGGAATCTGAACACGACCTCCTGGTCCACAAGGCTGTGGCC
AAATGGACGGCGGAAGAAGTTGTCCTCTGGCTGGAGCAGCTGGGCCCTTGGGCATCTCTT
TACAGGGAAAGGTTTTTATCTGAACGAGTAAATGGAAGGTTGCTTTTAACTTTGACAGAG
GAAGAATTTTCCAAGACGCCCTATACCATAGAAAACAGCAGCCACAGGAGAGCCATCCTC
ATGGAGCTAGAACGTGTCAAAGCATTAGGCGTGAAGCCCCCCCAGAATCTCTGGGAATAT
AAGGCTGTGAACCCAGGCAGGTCCCTGTTCCTGCTATACGCCCTCAAGAGCTCCCCCAGG
CTGAGTCTGCTCTACCTGTACCTGTTTGACTACACCGACACCTTCCTACCTTTCATCCAC
ACCATCTGCCCTCTGCAAGAAGACAGCTCTGGGGAGGACATCGTCACCAAGCTTCTGGAT
CTTAAGGAGCCTACGTGGAAGCAGTGGAGAGAGTTCCTGGTCAAATACTCCTTCCTTCCA
TACCAGCTGATTGCTGAGTTTGCTTGGGACTGGTTGGAGGTCCATTACTGGACATCACGG
TTTCTCATCATCAATGCTATGTTACTCTCAGTTCTGGAATTATTCTCCTTTTGGAGAATC
TGGTCGAGAAGTGAACTGAAGACCGTGCCTCAGAGGATGTGGAGCCATTTCTGGAAAGTA
TCAACGCAGGGGCTTTTTGTGGCCATGTTCTGGCCCCTCATCCCTCAGTTTGTTTGCAAC
TGTTTGTTTTACTGGGCCCTGTACTTTAACCCAATTATTAACATTGATCTTGTGGTCAAG
GAACTCCGGCGGCTGGAAACCCAGGTGTTG

ORF Protein

Cloned ORF protein sequence for pFN21AE1544 Download

>KIEE1544  450 aa
MEEPQKSYVNTMDLERDEPLKSTGPQISVSEFSCHCCYDILVNPTTLNCGHSFCRHCLAL
WWASSKKTECPECREKWEGFPKVSILLRDAIEKLFPDAIRLRFEDIQQNNDIVQSLAAFQ
KYGNDQIPLAPNTGRANQQMGGGFFSGVLTALTGVAVVLLVYHWSSRESEHDLLVHKAVA
KWTAEEVVLWLEQLGPWASLYRERFLSERVNGRLLLTLTEEEFSKTPYTIENSSHRRAIL
MELERVKALGVKPPQNLWEYKAVNPGRSLFLLYALKSSPRLSLLYLYLFDYTDTFLPFIH
TICPLQEDSSGEDIVTKLLDLKEPTWKQWREFLVKYSFLPYQLIAEFAWDWLEVHYWTSR
FLIINAMLLSVLELFSFWRIWSRSELKTVPQRMWSHFWKVSTQGLFVAMFWPLIPQFVCN
CLFYWALYFNPIINIDLVVKELRRLETQVL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1544 Download

>pFN21AE1544  6055 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGGAACCTCAGAAAAGCTATGTGAACACAAT
GGACCTTGAGAGAGATGAACCTCTCAAAAGCACCGGCCCTCAGATTTCTGTTAGTGAATT
TTCTTGCCACTGCTGCTACGACATCCTGGTTAACCCCACCACCTTGAACTGTGGGCACAG
CTTCTGCCGTCACTGCCTTGCTTTATGGTGGGCATCTTCAAAGAAAACAGAATGTCCAGA
ATGCAGAGAAAAATGGGAAGGTTTCCCCAAAGTCAGTATTCTCCTCAGGGATGCCATTGA
AAAGTTATTTCCTGATGCCATTAGACTGAGATTTGAAGACATTCAGCAGAATAATGACAT
AGTCCAAAGTCTTGCAGCCTTTCAGAAATATGGGAATGATCAGATTCCTTTAGCTCCTAA
CACAGGCCGAGCGAATCAGCAGATGGGAGGGGGATTCTTTTCCGGTGTGCTCACAGCTTT
AACTGGAGTGGCAGTGGTCCTGCTCGTCTATCACTGGAGCAGCAGGGAATCTGAACACGA
CCTCCTGGTCCACAAGGCTGTGGCCAAATGGACGGCGGAAGAAGTTGTCCTCTGGCTGGA
GCAGCTGGGCCCTTGGGCATCTCTTTACAGGGAAAGGTTTTTATCTGAACGAGTAAATGG
AAGGTTGCTTTTAACTTTGACAGAGGAAGAATTTTCCAAGACGCCCTATACCATAGAAAA
CAGCAGCCACAGGAGAGCCATCCTCATGGAGCTAGAACGTGTCAAAGCATTAGGCGTGAA
GCCCCCCCAGAATCTCTGGGAATATAAGGCTGTGAACCCAGGCAGGTCCCTGTTCCTGCT
ATACGCCCTCAAGAGCTCCCCCAGGCTGAGTCTGCTCTACCTGTACCTGTTTGACTACAC
CGACACCTTCCTACCTTTCATCCACACCATCTGCCCTCTGCAAGAAGACAGCTCTGGGGA
GGACATCGTCACCAAGCTTCTGGATCTTAAGGAGCCTACGTGGAAGCAGTGGAGAGAGTT
CCTGGTCAAATACTCCTTCCTTCCATACCAGCTGATTGCTGAGTTTGCTTGGGACTGGTT
GGAGGTCCATTACTGGACATCACGGTTTCTCATCATCAATGCTATGTTACTCTCAGTTCT
GGAATTATTCTCCTTTTGGAGAATCTGGTCGAGAAGTGAACTGAAGACCGTGCCTCAGAG
GATGTGGAGCCATTTCTGGAAAGTATCAACGCAGGGGCTTTTTGTGGCCATGTTCTGGCC
CCTCATCCCTCAGTTTGTTTGCAACTGTTTGTTTTACTGGGCCCTGTACTTTAACCCAAT
TATTAACATTGATCTTGTGGTCAAGGAACTCCGGCGGCTGGAAACCCAGGTGTTGGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT