(Circular) (Six-base) MAPSORT of: pFN21AE1544.seq Check: 8175 from: 1 to: 6055 pFN21AE1544 6055 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 1937 Size: 1461 4594 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3182 4118 5502 5770 1632 Size: 1550 936 1384 268 1917 Fragments arranged by size: 1917 1550 1384 936 268 AflII C'TTAA_G Cuts at: 820 1017 2966 820 Size: 197 1949 3909 Fragments arranged by size: 3909 1949 197 AflIII A'CryG_T Cuts at: 1203 2737 4954 5931 1203 Size: 1534 2217 977 1327 Fragments arranged by size: 2217 1534 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6055 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2505 2537 2505 Size: 32 6023 AlwNI CAG_nnn'CTG Cuts at: 2163 3798 5370 2163 Size: 1635 1572 2848 Fragments arranged by size: 2848 1635 1572 ApaI G_GGCC'C Cuts at: 2591 3283 2591 Size: 692 5363 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 2096 2669 3364 3590 3742 5851 1493 Size: 603 573 695 226 152 2109 1697 Fragments arranged by size: 2109 1697 695 603 573 226 152 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 3380 1870 Size: 73 1437 4545 Fragments arranged by size: 4545 1437 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3376 4831 618 Size: 325 217 627 1589 1455 1842 Fragments arranged by size: 1842 1589 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2591 2837 3283 3374 1391 Size: 1200 246 446 91 4072 Fragments arranged by size: 4072 1200 446 246 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 2931 3170 928 Size: 971 423 609 239 3813 Fragments arranged by size: 3813 971 609 423 239 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4246 4280 1903 Size: 34 2309 34 3678 Fragments arranged by size: 3678 2309 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 2377 1548 Size: 829 5226 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4666 136 Size: 107 122 71 631 605 2994 1525 Fragments arranged by size: 2994 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 2157 2591 3283 4116 5272 1790 Size: 367 434 692 833 1156 2573 Fragments arranged by size: 2573 1156 833 692 434 367 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3286 3786 4744 Size: 416 53 83 399 24 1869 500 958 Cuts at: 4744 442 Size: 1753 Fragments arranged by size: 1869 1753 958 500 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2738 2738 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2484 2515 2597 4715 1239 Size: 1245 31 82 2118 2579 Fragments arranged by size: 2579 2118 1245 82 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2066 2813 4180 5045 5343 2066 Size: 747 1367 865 298 2778 Fragments arranged by size: 2778 1367 865 747 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4718 882 Size: 617 3219 2219 Fragments arranged by size: 3219 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2979 3846 493 Size: 711 1775 867 2702 Fragments arranged by size: 2702 1775 867 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2681 3862 4244 4879 Size: 53 83 186 936 1148 1181 382 635 Cuts at: 4879 275 Size: 1451 Fragments arranged by size: 1451 1181 1148 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2441 4488 4886 5160 5307 5718 2441 Size: 2047 398 274 147 411 2778 Fragments arranged by size: 2778 2047 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2457 2487 2505 2535 2963 Size: 30 165 30 1054 30 18 30 428 Cuts at: 2963 2993 1178 Size: 30 4240 Fragments arranged by size: 4240 1054 428 165 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 1186 Size: 1066 4989 BseYI C'CCAG_C Cuts at: 2584 5258 5754 2584 Size: 2674 496 2885 Fragments arranged by size: 2885 2674 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5482 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 4266 5294 1540 Size: 275 186 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2451 2837 4116 4201 5272 2451 Size: 386 1279 85 1071 3234 Fragments arranged by size: 3234 1279 1071 386 85 BsmI GAATG_Cn' Cuts at: 2225 3566 3659 2225 Size: 1341 93 4621 Fragments arranged by size: 4621 1341 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2451 2591 2837 3283 3374 4116 Size: 399 367 294 140 246 446 91 742 Cuts at: 4116 4201 5272 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 446 399 367 294 246 140 91 85 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2793 3410 844 Size: 1949 617 3489 Fragments arranged by size: 3489 1949 617 BsrBI CCG'CTC Cuts at: 1394 1823 3942 1394 Size: 429 2119 3507 Fragments arranged by size: 3507 2119 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4550 4724 65 Size: 1183 3302 174 1396 Fragments arranged by size: 3302 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 4699 4886 1700 Size: 110 262 2627 187 2869 Fragments arranged by size: 2869 2627 262 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 4115 5127 4115 Size: 1012 5043 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstXI CCAn_nnnn'nTGG Cuts at: 2725 2959 2725 Size: 234 5821 BstYI r'GATC_y Cuts at: 1972 2962 3385 3752 4137 4154 5606 5692 5704 Size: 990 423 367 385 17 1452 86 12 Cuts at: 5704 6050 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 990 423 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2264 2264 Size: 6055 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4564 Fragments arranged by size: 4564 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 2107 2170 2478 2993 3571 4365 4385 2107 Size: 63 308 515 578 794 20 3777 Fragments arranged by size: 3777 794 578 515 308 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2525 5062 809 Size: 507 1209 2537 1802 Fragments arranged by size: 2537 1802 1209 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2541 3225 3502 Size: 54 1425 50 275 18 711 684 277 Cuts at: 3502 4391 6020 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 711 684 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3985 1353 Size: 2632 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2570 4642 5016 5162 387 Size: 1064 138 981 2072 374 146 1280 Fragments arranged by size: 2072 1280 1064 981 374 146 138 EcoICRI GAG'CTC Cuts at: 2835 2835 Size: 6055 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2484 2515 2597 3182 4118 4715 5502 Size: 393 852 31 82 585 936 597 787 Cuts at: 5502 5770 1239 Size: 268 1524 Fragments arranged by size: 1524 936 852 787 597 585 393 268 82 31 EcoNI CCTnn'n_nnAGG Cuts at: 2400 2400 Size: 6055 EcoO109I rG'GnC_Cy Cuts at: 2588 2805 3280 2588 Size: 217 475 5363 Fragments arranged by size: 5363 475 217 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 5202 1678 Size: 3524 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3157 3189 4696 4728 4770 Size: 32 135 32 1585 32 1507 32 42 Cuts at: 4770 4802 1373 Size: 32 2626 Fragments arranged by size: 2626 1585 1507 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2131 3399 3645 669 Size: 988 281 193 1268 246 3079 Fragments arranged by size: 3079 1268 988 281 246 193 HindIII A'AGCT_T Cuts at: 748 2954 748 Size: 2206 3849 HpaI GTT'AAC Cuts at: 2131 3645 2131 Size: 1514 4541 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3039 5169 5353 1305 Size: 1734 2130 184 2007 Fragments arranged by size: 2130 2007 1734 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2543 3227 10 Size: 54 1425 343 711 684 2838 Fragments arranged by size: 2838 1425 711 684 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2723 4013 4372 4531 518 Size: 648 1557 1290 359 159 2042 Fragments arranged by size: 2042 1557 1290 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2584 3031 3471 4148 5296 5541 1606 Size: 978 447 440 677 1148 245 2120 Fragments arranged by size: 2120 1148 978 677 447 440 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4564 Fragments arranged by size: 4564 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 3413 4958 1132 Size: 97 2184 1545 2229 Fragments arranged by size: 2229 2184 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 3234 3352 1108 Size: 2126 118 3811 Fragments arranged by size: 3811 2126 118 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2505 2537 4124 4156 5637 5669 2505 Size: 32 1587 32 1481 32 2891 Fragments arranged by size: 2891 1587 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2805 2805 Size: 6055 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 2587 3279 2587 Size: 692 5363 PstI C_TGCA'G Cuts at: 830 3407 830 Size: 2577 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2584 3031 2584 Size: 447 5608 SacI G_AGCT'C Cuts at: 2837 2837 Size: 6055 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 3403 4538 5219 5410 826 Size: 219 2358 1135 681 191 1471 Fragments arranged by size: 2358 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 2828 2966 4159 5060 5322 Size: 197 926 102 783 138 1193 901 262 Cuts at: 5322 820 Size: 1553 Fragments arranged by size: 1553 1193 926 901 783 262 197 138 102 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6055 StyI C'CwwG_G Cuts at: 513 1065 2004 2591 3494 513 Size: 552 939 587 903 3074 Fragments arranged by size: 3074 939 903 587 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4082 4099 4252 4437 4882 325 Size: 1255 2502 17 153 185 445 1498 Fragments arranged by size: 2502 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3285 4301 6013 Size: 274 80 33 51 1443 1308 1016 1712 Cuts at: 6013 96 Size: 138 Fragments arranged by size: 1712 1443 1308 1016 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2457 2489 2963 2995 6015 6047 2457 Size: 32 474 32 3020 32 2465 Fragments arranged by size: 3020 2465 474 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2339 2945 1316 Size: 1023 606 4426 Fragments arranged by size: 4426 1023 606 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 2319 4184 2319 Size: 1865 4190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI