VRK3 ORF clone pFN21AE0952

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0952

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Clone info.

Species

human

Clone Name

pFN21AE0952 Sequence (with vector) Restriction Map

Product ID

FHC11418

Flexi Clone PID

FXC11418

ORF Sequence

DNA sequence (1422 bp)
Protein sequence (474 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529006]

Gene

KIEE0952

Symbol

VRK3

Description

vaccinia related kinase 3, transcript variant 1

Original Clone ID

cp01452

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1422 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 474 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12791.1 2.7e-122 100.0 VRK3 CCDS77334.1 1.8e-103 99.8 VRK3 CCDS33076.1 7.4e-97 89.5 VRK3 CCDS9947.1 1.5e-23 36.2 VRK1 CCDS46291.1 3e-21 36.5 VRK2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017442 252 437 PF00069 Serine/threonine-protein kinase-like domain HMMSmart IPR002290 152 473 SM00220 Serine/threonine-protein kinase domain ProfileScan IPR000719 166 457 PS50011 Protein kinase Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0952 Download

>KIEE0952  1422 bp
ATGATCTCCTTCTGTCCAGACTGTGGCAAAAGTATCCAAGCGGCATTCAAATTCTGCCCC
TACTGTGGAAATTCTTTGCCTGTAGAGGAGCATGTAGGGTCCCAGACCTTTGTCAATCCA
CATGTGTCATCCTTCCAAGGCTCAAAGAGAGGGCTGAACTCCAGTTTTGAAACCTCTCCT
AAGAAAGTGAAATGGTCCAGCACCGTCACCTCTCCCCGATTATCCCTCTTCTCAGATGGT
GACAGTTCTGAGTCTGAAGATACTCTGAGTTCCTCTGAGAGATCCAAAGGCTCCGGGAGC
AGACCCCCAACCCCCAAAAGCAGCCCTCAGAAGACCAGGAAGAGCCCTCAGGTGACCAGG
GGTAGCCCTCAGAAGACCAGCTGTAGCCCTCAGAAGACCAGGCAGAGCCCTCAGACGCTG
AAGCGGAGCCGAGTGACCACCTCACTTGAAGCTTTGCCCACAGGGACAGTGCTGACAGAC
AAGAGTGGGCGACAGTGGAAGCTGAAGTCCTTCCAGACCAGGGACAACCAGGGCATTCTC
TATGAAGCTGCACCCACCTCCACCCTCACCTGTGACTCAGGACCACAGAAGCAAAAGTTC
TCACTCAAACTGGATGCCAAGGATGGGCGCTTGTTCAATGAGCAGAACTTCTTCCAGCGG
GCCGCCAAGCCTCTGCAAGTCAACAAGTGGAAGAAGCTGTACTCGACCCCACTGCTGGCC
ATCCCTACCTGCATGGGTTTCGGTGTTCACCAGGACAAATACAGGTTCTTGGTGTTACCC
AGCCTGGGGAGGAGCCTTCAGTCGGCCCTGGATGTCAGCCCAAAGCATGTGCTGTCAGAG
AGGTCTGTGCTGCAGGTGGCCTGCCGGCTGCTGGATGCCCTGGAGTTCCTCCATGAGAAT
GAGTATGTTCATGGAAATGTGACAGCTGAAAATATCTTTGTGGATCCAGAGGACCAGAGT
CAGGTGACTTTGGCAGGCTATGGCTTCGCCTTCCGCTATTGCCCAAGTGGCAAACACGTG
GCCTACGTGGAAGGCAGCAGGAGCCCTCACGAGGGGGACCTTGAGTTCATTAGCATGGAC
CTGCACAAGGGATGCGGGCCCTCCCGCCGCAGCGACCTCCAGAGCCTGGGCTACTGCATG
CTGAAGTGGCTCTACGGGTTTCTGCCATGGACAAATTGCCTTCCCAACACTGAGGACATC
ATGAAGCAAAAACAGAAGTTTGTTGATAAGCCGGGGCCCTTCGTGGGACCCTGCGGTCAC
TGGATCAGGCCCTCAGAGACCCTGCAGAAGTACCTGAAGGTGGTGATGGCCCTCACGTAT
GAGGAGAAGCCGCCCTACGCCATGCTGAGGAACAACCTAGAAGCTTTGCTGCAGGATCTG
CGTGTGTCTCCATATGACCCCATTGGCCTCCCGATGGTGCCC

ORF Protein

Cloned ORF protein sequence for pFN21AE0952 Download

>KIEE0952  474 aa
MISFCPDCGKSIQAAFKFCPYCGNSLPVEEHVGSQTFVNPHVSSFQGSKRGLNSSFETSP
KKVKWSSTVTSPRLSLFSDGDSSESEDTLSSSERSKGSGSRPPTPKSSPQKTRKSPQVTR
GSPQKTSCSPQKTRQSPQTLKRSRVTTSLEALPTGTVLTDKSGRQWKLKSFQTRDNQGIL
YEAAPTSTLTCDSGPQKQKFSLKLDAKDGRLFNEQNFFQRAAKPLQVNKWKKLYSTPLLA
IPTCMGFGVHQDKYRFLVLPSLGRSLQSALDVSPKHVLSERSVLQVACRLLDALEFLHEN
EYVHGNVTAENIFVDPEDQSQVTLAGYGFAFRYCPSGKHVAYVEGSRSPHEGDLEFISMD
LHKGCGPSRRSDLQSLGYCMLKWLYGFLPWTNCLPNTEDIMKQKQKFVDKPGPFVGPCGH
WIRPSETLQKYLKVVMALTYEEKPPYAMLRNNLEALLQDLRVSPYDPIGLPMVP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0952 Download

>pFN21AE0952  6127 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATCTCCTTCTGTCCAGACTGTGGCAAAAGTAT
CCAAGCGGCATTCAAATTCTGCCCCTACTGTGGAAATTCTTTGCCTGTAGAGGAGCATGT
AGGGTCCCAGACCTTTGTCAATCCACATGTGTCATCCTTCCAAGGCTCAAAGAGAGGGCT
GAACTCCAGTTTTGAAACCTCTCCTAAGAAAGTGAAATGGTCCAGCACCGTCACCTCTCC
CCGATTATCCCTCTTCTCAGATGGTGACAGTTCTGAGTCTGAAGATACTCTGAGTTCCTC
TGAGAGATCCAAAGGCTCCGGGAGCAGACCCCCAACCCCCAAAAGCAGCCCTCAGAAGAC
CAGGAAGAGCCCTCAGGTGACCAGGGGTAGCCCTCAGAAGACCAGCTGTAGCCCTCAGAA
GACCAGGCAGAGCCCTCAGACGCTGAAGCGGAGCCGAGTGACCACCTCACTTGAAGCTTT
GCCCACAGGGACAGTGCTGACAGACAAGAGTGGGCGACAGTGGAAGCTGAAGTCCTTCCA
GACCAGGGACAACCAGGGCATTCTCTATGAAGCTGCACCCACCTCCACCCTCACCTGTGA
CTCAGGACCACAGAAGCAAAAGTTCTCACTCAAACTGGATGCCAAGGATGGGCGCTTGTT
CAATGAGCAGAACTTCTTCCAGCGGGCCGCCAAGCCTCTGCAAGTCAACAAGTGGAAGAA
GCTGTACTCGACCCCACTGCTGGCCATCCCTACCTGCATGGGTTTCGGTGTTCACCAGGA
CAAATACAGGTTCTTGGTGTTACCCAGCCTGGGGAGGAGCCTTCAGTCGGCCCTGGATGT
CAGCCCAAAGCATGTGCTGTCAGAGAGGTCTGTGCTGCAGGTGGCCTGCCGGCTGCTGGA
TGCCCTGGAGTTCCTCCATGAGAATGAGTATGTTCATGGAAATGTGACAGCTGAAAATAT
CTTTGTGGATCCAGAGGACCAGAGTCAGGTGACTTTGGCAGGCTATGGCTTCGCCTTCCG
CTATTGCCCAAGTGGCAAACACGTGGCCTACGTGGAAGGCAGCAGGAGCCCTCACGAGGG
GGACCTTGAGTTCATTAGCATGGACCTGCACAAGGGATGCGGGCCCTCCCGCCGCAGCGA
CCTCCAGAGCCTGGGCTACTGCATGCTGAAGTGGCTCTACGGGTTTCTGCCATGGACAAA
TTGCCTTCCCAACACTGAGGACATCATGAAGCAAAAACAGAAGTTTGTTGATAAGCCGGG
GCCCTTCGTGGGACCCTGCGGTCACTGGATCAGGCCCTCAGAGACCCTGCAGAAGTACCT
GAAGGTGGTGATGGCCCTCACGTATGAGGAGAAGCCGCCCTACGCCATGCTGAGGAACAA
CCTAGAAGCTTTGCTGCAGGATCTGCGTGTGTCTCCATATGACCCCATTGGCCTCCCGAT
GGTGCCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT