(Circular) (Six-base) MAPSORT of: pFN21AE0952.seq Check: 3647 from: 1 to: 6127 pFN21AE0952 6127 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2848 2848 Size: 6127 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3470 1937 Size: 1533 4594 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2280 2444 2528 2786 3167 3320 4190 5574 Size: 648 164 84 258 381 153 870 1384 Cuts at: 5574 5842 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 870 648 381 268 258 164 153 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 2125 3019 5026 6003 1203 Size: 922 894 2007 977 1327 Fragments arranged by size: 2007 1327 977 922 894 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3101 1184 Size: 1917 4210 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3053 3085 3053 Size: 32 6095 AlwNI CAG_nnn'CTG Cuts at: 2422 3130 3382 3870 5442 2422 Size: 708 252 488 1572 3107 Fragments arranged by size: 3107 1572 708 488 252 ApaI G_GGCC'C Cuts at: 3105 3243 3105 Size: 138 5989 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 2054 2074 3436 3662 3814 5923 1493 Size: 561 20 1362 226 152 2109 1697 Fragments arranged by size: 2109 1697 1362 561 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3452 1870 Size: 73 1509 4545 Fragments arranged by size: 4545 1509 73 BamHI G'GATC_C Cuts at: 2947 3457 3824 2947 Size: 510 367 5250 Fragments arranged by size: 5250 510 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3421 3448 4903 618 Size: 325 217 627 1634 27 1455 1842 Fragments arranged by size: 1842 1634 1455 627 325 217 27 BanII G_rGCy'C Cuts at: 1391 2351 2414 3050 3105 3243 3446 1391 Size: 960 63 636 55 138 203 4072 Fragments arranged by size: 4072 960 636 203 138 63 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2342 2384 2405 928 Size: 971 443 42 21 4650 Fragments arranged by size: 4650 971 443 42 21 BbvCI CC'TCA_GC Cuts at: 3350 3350 Size: 6127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2698 2725 2732 2759 4318 4352 1903 Size: 34 761 27 7 27 1559 34 3678 Fragments arranged by size: 3678 1559 761 34 34 27 27 7 BciVI GTATCCnnnnn_n' Cuts at: 2048 4019 5229 2048 Size: 1971 1210 2946 Fragments arranged by size: 2946 1971 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3012 4738 136 Size: 107 122 71 631 605 1340 1726 1525 Fragments arranged by size: 1726 1525 1340 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 3105 3243 3426 4188 5344 1790 Size: 1315 138 183 762 1156 2573 Fragments arranged by size: 2573 1315 1156 762 183 138 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3858 4816 442 Size: 416 53 83 399 24 2441 958 1753 Fragments arranged by size: 2441 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2149 2906 3107 4787 1239 Size: 910 757 201 1680 2579 Fragments arranged by size: 2579 1680 910 757 201 Bpu10I CC'TnA_GC Cuts at: 3350 3350 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3086 4252 5117 5415 3086 Size: 1166 865 298 3798 Fragments arranged by size: 3798 1166 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3275 4790 882 Size: 617 1776 1515 2219 Fragments arranged by size: 2219 1776 1515 617 BsaAI yAC'GTr Cuts at: 493 1204 3022 3031 3321 3918 493 Size: 711 1818 9 290 597 2702 Fragments arranged by size: 2702 1818 711 597 290 9 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5902 Fragments arranged by size: 5902 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2809 3341 1186 Size: 919 704 532 3972 Fragments arranged by size: 3972 919 704 532 BseYI C'CCAG_C Cuts at: 2783 5330 5826 2783 Size: 2547 496 3084 Fragments arranged by size: 3084 2547 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2538 3071 1163 Size: 573 802 533 4219 Fragments arranged by size: 4219 802 573 533 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4188 4273 5344 4188 Size: 85 1071 4971 Fragments arranged by size: 4971 1071 85 BsmI GAATG_Cn' Cuts at: 2048 2538 3638 3731 2048 Size: 490 1100 93 4444 Fragments arranged by size: 4444 1100 490 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2351 2414 3050 3105 3243 3426 3446 Size: 399 561 63 636 55 138 183 20 Cuts at: 3446 4188 4273 5344 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 636 561 399 183 138 85 63 55 20 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 3204 4016 5746 3204 Size: 812 1730 3585 Fragments arranged by size: 3585 1730 812 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2741 2848 3093 3482 844 Size: 1897 107 245 389 3489 Fragments arranged by size: 3489 1897 389 245 107 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4622 4796 65 Size: 1183 3374 174 1396 Fragments arranged by size: 3374 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2868 4771 4958 1700 Size: 110 1058 1903 187 2869 Fragments arranged by size: 2869 1903 1058 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 3053 4187 5199 3053 Size: 1134 1012 3981 Fragments arranged by size: 3981 1134 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3382 794 Size: 2588 3539 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 2356 1184 Size: 1172 4955 BstYI r'GATC_y Cuts at: 1972 2285 2947 3379 3457 3824 4209 4226 5678 Size: 313 662 432 78 367 385 17 1452 Cuts at: 5678 5764 5776 6122 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 662 432 385 367 346 313 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2352 2352 Size: 6127 BtgI C'CryG_G Cuts at: 513 1065 3170 513 Size: 552 2105 3470 Fragments arranged by size: 3470 2105 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 2714 3643 4437 4457 2714 Size: 929 794 20 4384 Fragments arranged by size: 4384 929 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2431 2466 529 Size: 35 1867 35 4190 Fragments arranged by size: 4190 1867 35 35 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3258 5134 809 Size: 507 1942 1876 1802 Fragments arranged by size: 1942 1876 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2721 3574 4463 Size: 54 1425 50 275 18 891 853 889 Cuts at: 4463 6092 8 Size: 1629 43 Fragments arranged by size: 1629 1425 891 889 853 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2339 4057 1353 Size: 884 102 1718 3423 Fragments arranged by size: 3423 1718 884 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4714 5088 5234 387 Size: 1064 138 3125 374 146 1280 Fragments arranged by size: 3125 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 2280 2444 2528 2786 2906 3107 Size: 393 517 131 164 84 258 120 201 Cuts at: 3107 3167 3320 4190 4787 5574 5842 1239 Size: 60 153 870 597 787 268 1524 Fragments arranged by size: 1524 870 787 597 517 393 268 258 201 164 153 131 120 84 60 EcoNI CCTnn'n_nnAGG Cuts at: 3089 3089 Size: 6127 EcoO109I rG'GnC_Cy Cuts at: 2103 3061 3102 3239 3240 3251 3273 2103 Size: 958 41 137 1 11 22 4957 Fragments arranged by size: 4957 958 137 41 22 11 1 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 2635 5274 1678 Size: 957 2639 2531 Fragments arranged by size: 2639 2531 957 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2214 2246 4768 4800 4842 Size: 32 135 32 642 32 2522 32 42 Cuts at: 4842 4874 1373 Size: 32 2626 Fragments arranged by size: 2626 2522 642 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2686 3471 3717 669 Size: 988 281 748 785 246 3079 Fragments arranged by size: 3079 988 785 748 281 246 HindIII A'AGCT_T Cuts at: 748 2454 3366 748 Size: 1706 912 3509 Fragments arranged by size: 3509 1706 912 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5241 5425 1305 Size: 3936 184 2007 Fragments arranged by size: 3936 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2723 10 Size: 54 1425 343 891 3414 Fragments arranged by size: 3414 1425 891 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2901 2919 3077 4085 4444 4603 518 Size: 648 1735 18 158 1008 359 159 2042 Fragments arranged by size: 2042 1735 1008 648 359 159 158 18 MspA1I CmG'CkG Cuts at: 1606 2385 2662 2930 3543 4220 5368 5613 1606 Size: 779 277 268 613 677 1148 245 2120 Fragments arranged by size: 2120 1148 779 677 613 277 268 245 NaeI GCC'GGC Cuts at: 2870 2870 Size: 6127 NcoI C'CATG_G Cuts at: 513 1065 3170 513 Size: 552 2105 3470 Fragments arranged by size: 3470 2105 552 NdeI CA'TA_TG Cuts at: 387 3397 387 Size: 3010 3117 NgoMIV G'CCGG_C Cuts at: 2868 2868 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2099 2129 2834 3145 3485 5030 1132 Size: 97 870 30 705 311 340 1545 2229 Fragments arranged by size: 2229 1545 870 705 340 311 97 30 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 2125 5026 2125 Size: 2901 3226 PflMI CCAn_nnn'nTGG Cuts at: 1108 2027 3130 1108 Size: 919 1103 4105 Fragments arranged by size: 4105 1103 919 PfoI T'CCnGG_A Cuts at: 2297 2297 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PmlI CAC'GTG Cuts at: 3022 3022 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2103 3061 3251 2103 Size: 958 190 4979 Fragments arranged by size: 4979 958 190 PshAI GACnn'nnGTC Cuts at: 1613 2114 2961 1613 Size: 501 847 4779 Fragments arranged by size: 4779 847 501 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspOMI G'GGCC_C Cuts at: 3101 3239 3101 Size: 138 5989 PstI C_TGCA'G Cuts at: 830 2859 3291 3378 3479 830 Size: 2029 432 87 101 3478 Fragments arranged by size: 3478 2029 432 101 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2385 2930 2385 Size: 545 5582 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SanDI GG'GwC_CC Cuts at: 2103 3251 2103 Size: 1148 4979 SapI GCTCTTCn'nnn_ Cuts at: 2339 2339 Size: 6127 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SfcI C'TryA_G Cuts at: 826 1045 2085 2386 2855 3287 3374 3475 4610 Size: 219 1040 301 469 432 87 101 1135 Cuts at: 4610 5291 5482 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1040 681 469 432 301 219 191 101 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3065 4231 5132 5394 820 Size: 197 926 1122 1166 901 262 1553 Fragments arranged by size: 1553 1166 1122 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3145 3485 1132 Size: 2013 340 3774 Fragments arranged by size: 3774 2013 340 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2140 2622 3170 3566 513 Size: 552 1075 482 548 396 3074 Fragments arranged by size: 3074 1075 552 548 482 396 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2572 4154 4171 4324 4509 4954 325 Size: 1255 992 1582 17 153 185 445 1498 Fragments arranged by size: 1582 1498 1255 992 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2703 4373 6085 Size: 274 80 33 51 1443 726 1670 1712 Cuts at: 6085 96 Size: 138 Fragments arranged by size: 1712 1670 1443 726 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6087 6119 6087 Size: 32 6095 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 2513 2654 4256 2513 Size: 141 1602 4384 Fragments arranged by size: 4384 1602 141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI