EVL ORF clone pFN21AE0936

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Halotag ORF Clone pFN21AE0936

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Clone info.

Species

human

Clone Name

pFN21AE0936 Sequence (with vector) Restriction Map

Product ID

FHC11408

Flexi Clone PID

FXC11408

ORF Sequence

DNA sequence (1254 bp)
Protein sequence (418 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528996]

Gene

KIEE0936

Symbol

EVL

Alias : RNB6

Description

Enah/Vasp-like

Original Clone ID

cp01436

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1254 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 418 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9955.1 4.3e-62 100.0 EVL CCDS81851.1 1.1e-61 100.0 EVL CCDS33051.1 5.4e-18 48.0 VASP CCDS31040.1 1.1e-11 39.2 ENAH CCDS31041.1 1.1e-11 39.2 ENAH The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000697 6 107 PF00568 EVH1 NULL 217 244 PF11643 NULL IPR014885 378 417 PF08776 VASP tetramerisation HMMSmart IPR000697 3 111 SM00461 EVH1 ProfileScan IPR000697 1 114 PS50229 EVH1
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057421 418 616912 100.0 100.0 NP_001317150 416 616912 100.0 99.3 XP_005267806 422 616912 100.0 99.3 XP_016876852 429 616912 84.5 88.0 XP_011535130 370 616912 84.5 87.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0936 Download

>KIEE0936  1254 bp
ATGGCCACAAGTGAACAGAGTATCTGCCAAGCCCGGGCTTCCGTGATGGTCTACGATGAC
ACCAGTAAGAAATGGGTACCAATCAAACCTGGCCAGCAGGGATTCAGCCGGATCAACATC
TACCACAACACTGCCAGCAACACCTTCAGAGTCGTTGGAGTCAAGTTGCAGGATCAGCAG
GTTGTGATCAATTATTCAATCGTGAAAGGGCTGAAGTACAATCAGGCCACGCCAACCTTC
CACCAGTGGCGAGATGCCCGCCAGGTCTACGGCTTAAACTTTGCAAGTAAAGAAGAGGCA
ACCACGTTCTCCAATGCAATGCTGTTTGCCCTGAACATCATGAATTCCCAAGAAGGAGGC
CCCTCCAGCCAGCGTCAGGTGCAGAATGGCCCCTCTCCTGATGAGATGGACATCCAGAGA
AGACAAGTGATGGAGCAGCACCAGCAGCAGCGTCAGGAATCTCTAGAAAGAAGAACCTCG
GCCACAGGGCCCATCCTCCCACCAGGACATCCTTCATCTGCAGCCAGCGCCCCCGTCTCA
TGTAGTGGGCCTCCACCGCCCCCCCCACCCCCAGTCCCACCTCCACCCACTGGGGCTACC
CCACCTCCCCCACCCCCACTGCCAGCCGGAGGAGCCCAGGGGTCCAGCCACGACGAGAGC
TCCATGTCAGGACTGGCCGCTGCCATAGCTGGGGCCAAGCTGAGAAGAGTCCAACGGCCA
GAAGACGCATCTGGAGGCTCCAGTCCCAGTGGGACCTCAAAGTCCGATGCCAACCGGGCA
AGCAGCGGGGGTGGCGGAGGAGGCCTCATGGAGGAAATGAACAAACTGCTGGCCAAGAGG
AGAAAAGCAGCCTCCCAGTCAGACAAGCCAGCCGAGAAGAAGGAAGATGAAAGCCAAATG
GAAGATCCTAGTACCTCCCCCTCTCCGGGGACCCGAGCAGCCAGCCAGCCACCTAACTCC
TCAGAGGCTGGCCGGAAGCCCTGGGAGCGGAGCAACTCGGTGGAGAAGCCTGTGTCCTCG
ATTCTGTCCAGAACCCCGTCTGTGGCAAAGAGCCCCGAAGCTAAGAGCCCCCTTCAGTCG
CAGCCTCACTCTAGGATGAAGCCTGCTGGGAGCGTGAATGACATGGCCCTGGATGCCTTC
GACTTGGACCGGATGAAGCAGGAGATCCTAGAGGAGGTGGTGAGAGAGCTCCACAAGGTG
AAGGAGGAGATCATCGACGCCATCAGGCAGGAGCTGAGTGGGATCAGCACCACG

ORF Protein

Cloned ORF protein sequence for pFN21AE0936 Download

>KIEE0936  418 aa
MATSEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTASNTFRVVGVKLQDQQ
VVINYSIVKGLKYNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQEGG
PSSQRQVQNGPSPDEMDIQRRQVMEQHQQQRQESLERRTSATGPILPPGHPSSAASAPVS
CSGPPPPPPPPVPPPPTGATPPPPPPLPAGGAQGSSHDESSMSGLAAAIAGAKLRRVQRP
EDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNKLLAKRRKAASQSDKPAEKKEDESQM
EDPSTSPSPGTRAASQPPNSSEAGRKPWERSNSVEKPVSSILSRTPSVAKSPEAKSPLQS
QPHSRMKPAGSVNDMALDAFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0936 Download

>pFN21AE0936  5959 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCACAAGTGAACAGAGTATCTGCCAAGCCCG
GGCTTCCGTGATGGTCTACGATGACACCAGTAAGAAATGGGTACCAATCAAACCTGGCCA
GCAGGGATTCAGCCGGATCAACATCTACCACAACACTGCCAGCAACACCTTCAGAGTCGT
TGGAGTCAAGTTGCAGGATCAGCAGGTTGTGATCAATTATTCAATCGTGAAAGGGCTGAA
GTACAATCAGGCCACGCCAACCTTCCACCAGTGGCGAGATGCCCGCCAGGTCTACGGCTT
AAACTTTGCAAGTAAAGAAGAGGCAACCACGTTCTCCAATGCAATGCTGTTTGCCCTGAA
CATCATGAATTCCCAAGAAGGAGGCCCCTCCAGCCAGCGTCAGGTGCAGAATGGCCCCTC
TCCTGATGAGATGGACATCCAGAGAAGACAAGTGATGGAGCAGCACCAGCAGCAGCGTCA
GGAATCTCTAGAAAGAAGAACCTCGGCCACAGGGCCCATCCTCCCACCAGGACATCCTTC
ATCTGCAGCCAGCGCCCCCGTCTCATGTAGTGGGCCTCCACCGCCCCCCCCACCCCCAGT
CCCACCTCCACCCACTGGGGCTACCCCACCTCCCCCACCCCCACTGCCAGCCGGAGGAGC
CCAGGGGTCCAGCCACGACGAGAGCTCCATGTCAGGACTGGCCGCTGCCATAGCTGGGGC
CAAGCTGAGAAGAGTCCAACGGCCAGAAGACGCATCTGGAGGCTCCAGTCCCAGTGGGAC
CTCAAAGTCCGATGCCAACCGGGCAAGCAGCGGGGGTGGCGGAGGAGGCCTCATGGAGGA
AATGAACAAACTGCTGGCCAAGAGGAGAAAAGCAGCCTCCCAGTCAGACAAGCCAGCCGA
GAAGAAGGAAGATGAAAGCCAAATGGAAGATCCTAGTACCTCCCCCTCTCCGGGGACCCG
AGCAGCCAGCCAGCCACCTAACTCCTCAGAGGCTGGCCGGAAGCCCTGGGAGCGGAGCAA
CTCGGTGGAGAAGCCTGTGTCCTCGATTCTGTCCAGAACCCCGTCTGTGGCAAAGAGCCC
CGAAGCTAAGAGCCCCCTTCAGTCGCAGCCTCACTCTAGGATGAAGCCTGCTGGGAGCGT
GAATGACATGGCCCTGGATGCCTTCGACTTGGACCGGATGAAGCAGGAGATCCTAGAGGA
GGTGGTGAGAGAGCTCCACAAGGTGAAGGAGGAGATCATCGACGCCATCAGGCAGGAGCT
GAGTGGGATCAGCACCACGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT