(Circular) (Six-base) MAPSORT of: pFN21AE0936.seq Check: 8170 from: 1 to: 5959 pFN21AE0936 5959 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2055 2271 3302 1937 Size: 118 216 1031 4594 Fragments arranged by size: 4594 1031 216 118 Acc65I G'GTAC_C Cuts at: 2080 3280 2080 Size: 1200 4759 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2134 2237 3062 4022 5406 5674 1632 Size: 502 103 825 960 1384 268 1917 Fragments arranged by size: 1917 1384 960 825 502 268 103 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 2972 3238 3702 5274 2972 Size: 266 464 1572 3657 Fragments arranged by size: 3657 1572 464 266 ApaI G_GGCC'C Cuts at: 2496 2496 Size: 5959 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2347 3268 3494 3646 5755 1493 Size: 854 921 226 152 2109 1697 Fragments arranged by size: 2109 1697 921 854 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2037 2937 3284 1870 Size: 73 94 900 347 4545 Fragments arranged by size: 4545 900 347 94 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 3280 4735 618 Size: 325 217 627 293 1200 1455 1842 Fragments arranged by size: 1842 1455 1200 627 325 293 217 BanII G_rGCy'C Cuts at: 1391 2496 2641 2666 3059 3074 3195 3278 1391 Size: 1105 145 25 393 15 121 83 4072 Fragments arranged by size: 4072 1105 393 145 121 83 25 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2431 2733 928 Size: 971 532 302 4154 Fragments arranged by size: 4154 971 532 302 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2190 1548 Size: 642 5317 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2780 4570 136 Size: 107 122 71 631 605 1108 1790 1525 Fragments arranged by size: 1790 1525 1108 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2496 4020 5176 1790 Size: 706 1524 1156 2573 Fragments arranged by size: 2573 1524 1156 706 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2570 2604 2745 Size: 416 53 83 399 24 1153 34 141 Cuts at: 2745 2854 3690 4648 442 Size: 109 836 958 1753 Fragments arranged by size: 1753 1153 958 836 416 399 141 109 83 53 34 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 2825 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2353 2728 2757 4619 1239 Size: 1114 375 29 1862 2579 Fragments arranged by size: 2579 1862 1114 375 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 2120 2414 5953 2120 Size: 294 3539 2126 Fragments arranged by size: 3539 2126 294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3222 3766 4148 4783 Size: 53 83 186 936 1689 544 382 635 Cuts at: 4783 275 Size: 1451 Fragments arranged by size: 1689 1451 936 635 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3153 4392 4790 5064 5211 5622 3153 Size: 1239 398 274 147 411 3490 Fragments arranged by size: 3490 1239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2593 2623 2648 2678 1178 Size: 30 165 30 1190 30 25 30 4459 Fragments arranged by size: 4459 1190 165 30 30 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2649 2817 2857 2953 3191 3224 1186 Size: 1463 168 40 96 238 33 3921 Fragments arranged by size: 3921 1463 238 168 96 40 33 BseYI C'CCAG_C Cuts at: 2693 3110 5162 5658 2693 Size: 417 2052 496 2994 Fragments arranged by size: 2994 2052 496 417 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2405 1163 Size: 573 669 4717 Fragments arranged by size: 4717 669 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2666 3195 4020 4105 5176 2666 Size: 529 825 85 1071 3449 Fragments arranged by size: 3449 1071 825 529 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 BsmBI CGTCTCn'nnnn_ Cuts at: 2545 2545 Size: 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2496 2641 2666 3059 3074 3195 3278 Size: 399 706 145 25 393 15 121 83 Cuts at: 3278 4020 4105 5176 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 706 399 393 145 121 85 83 25 15 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2343 3848 5578 2343 Size: 1505 1730 2724 Fragments arranged by size: 2724 1730 1505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2173 3314 844 Size: 1329 1141 3489 Fragments arranged by size: 3489 1329 1141 BsrBI CCG'CTC Cuts at: 1394 1823 2992 3846 1394 Size: 429 1169 854 3507 Fragments arranged by size: 3507 1169 854 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2328 4454 4628 65 Size: 1183 1080 2126 174 1396 Fragments arranged by size: 2126 1396 1183 1080 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2908 3168 3289 3656 4041 4058 5510 5596 Size: 936 260 121 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 936 385 367 346 260 121 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3255 513 Size: 552 939 1251 3217 Fragments arranged by size: 3217 1251 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 2133 2621 3475 4269 4289 2133 Size: 488 854 794 20 3803 Fragments arranged by size: 3803 854 794 488 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DraIII CAC_nnn'GTG Cuts at: 3202 3202 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2764 4966 809 Size: 507 1448 2202 1802 Fragments arranged by size: 2202 1802 1448 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2095 2484 Size: 54 1425 50 275 18 177 88 389 Cuts at: 2484 2679 2720 2835 2974 3406 4295 5924 8 Size: 195 41 115 139 432 889 1629 43 Fragments arranged by size: 1629 1425 889 432 389 275 195 177 139 115 88 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2292 2704 3889 1353 Size: 939 412 1185 3423 Fragments arranged by size: 3423 1185 939 412 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2814 4546 4920 5066 387 Size: 1064 138 1225 1732 374 146 1280 Fragments arranged by size: 1732 1280 1225 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2664 3193 2664 Size: 529 5430 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2134 2237 2353 2728 2757 3062 4022 Size: 393 502 103 116 375 29 305 960 Cuts at: 4022 4619 5406 5674 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 960 787 597 502 393 375 305 268 116 103 29 EcoNI CCTnn'n_nnAGG Cuts at: 2813 3176 2813 Size: 363 5596 EcoO109I rG'GnC_Cy Cuts at: 2363 2492 2757 2934 2363 Size: 129 265 177 5388 Fragments arranged by size: 5388 265 177 129 EcoRI G'AATT_C Cuts at: 2347 3268 3646 2347 Size: 921 378 4660 Fragments arranged by size: 4660 921 378 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2535 5106 1678 Size: 857 2571 2531 Fragments arranged by size: 2571 2531 857 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 4600 4632 4674 Size: 32 135 32 1076 32 1920 32 42 Cuts at: 4674 4706 1373 Size: 32 2626 Fragments arranged by size: 2626 1920 1076 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 2084 3284 2084 Size: 1200 4759 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2140 2740 5073 5257 1305 Size: 835 600 2333 184 2007 Fragments arranged by size: 2333 2007 835 600 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2097 2837 10 Size: 54 1425 343 177 88 740 3132 Fragments arranged by size: 3132 1425 740 343 177 88 54 MslI CAynn'nnrTG Cuts at: 518 1166 3917 4276 4435 518 Size: 648 2751 359 159 2042 Fragments arranged by size: 2751 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2684 2790 3375 4052 5200 5445 1606 Size: 1078 106 585 677 1148 245 2120 Fragments arranged by size: 2120 1148 1078 677 585 245 106 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4468 Fragments arranged by size: 4468 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 2641 2985 1348 Size: 1293 344 4322 Fragments arranged by size: 4322 1293 344 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 2594 1108 Size: 1486 4473 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2757 2934 2757 Size: 177 5782 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2492 2492 Size: 5959 PstI C_TGCA'G Cuts at: 830 2527 3311 830 Size: 1697 784 3478 Fragments arranged by size: 3478 1697 784 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SacI G_AGCT'C Cuts at: 2666 3195 2666 Size: 529 5430 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SanDI GG'GwC_CC Cuts at: 2934 2934 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2523 3307 4442 5123 5314 826 Size: 219 1478 784 1135 681 191 1471 Fragments arranged by size: 1478 1471 1135 784 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 2039 3286 1872 Size: 167 1247 4545 Fragments arranged by size: 4545 1247 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 4063 4964 5226 820 Size: 197 926 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SrfI GCCC'GGGC Cuts at: 2039 2039 Size: 5959 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 2808 1517 Size: 1291 4668 StyI C'CwwG_G Cuts at: 513 1065 2004 3398 513 Size: 552 939 1394 3074 Fragments arranged by size: 3074 1394 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2605 3986 4003 4156 4341 4786 325 Size: 1255 1025 1381 17 153 185 445 1498 Fragments arranged by size: 1498 1381 1255 1025 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2220 4205 5917 Size: 274 80 33 51 1443 243 1985 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1985 1712 1443 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2562 2594 5919 5951 2562 Size: 32 3325 32 2570 Fragments arranged by size: 3325 2570 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 2467 3295 2467 Size: 828 5131 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2674 2674 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 2037 3284 1870 Size: 167 1247 4545 Fragments arranged by size: 4545 1247 167 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SwaI