KLF8 ORF clone pFN21AE0926

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Halotag ORF Clone pFN21AE0926

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Clone info.

Species

human

Clone Name

pFN21AE0926 Sequence (with vector) Restriction Map

Product ID

FHC11403

Flexi Clone PID

FXC11403

ORF Sequence

DNA sequence (1077 bp)
Protein sequence (359 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528991]

Gene

KIEE0926

Symbol

KLF8

Alias : BKLF3, ZNF741

Description

Kruppel-like factor 8, transcript variant 1

Original Clone ID

cp01426

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1077 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 359 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14373.1 1.8e-67 100.0 KLF8 CCDS55428.1 1.8e-44 98.8 KLF8 CCDS3444.1 2.9e-15 38.3 KLF3 CCDS9449.1 2.1e-13 44.4 KLF12 CCDS66562.1 5.1e-13 35.3 KLF5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007087 334 356 PF00096 Zinc finger HMMSmart IPR015880 274 298 SM00355 Zinc finger IPR015880 304 328 SM00355 Zinc finger IPR015880 334 356 SM00355 Zinc finger ProfileScan IPR007087 274 303 PS50157 Zinc finger IPR007087 304 333 PS50157 Zinc finger IPR007087 334 359 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001311031 359 300286 100.0 100.0 NP_009181 359 300286 100.0 100.0 XP_005262034 359 300286 100.0 100.0 XP_005262036 359 300286 100.0 100.0 NP_001311033 364 300286 100.0 99.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0926 Download

>KIEE0926  1077 bp
ATGGTCGATATGGATAAACTCATAAACAACTTGGAGGTCCAACTTAATTCAGAAGGTGGC
TCAATGCAGGTATTCAAGCAGGTCACTGCTTCTGTTCGGAACAGAGATCCCCCTGAGATA
GAATACAGAAGTAATATGACTTCTCCAACACTCCTGGATGCCAACCCCATGGAGAACCCA
GCACTGTTTAATGACATCAAGATTGAGCCCCCAGAAGAACTTTTGGCTAGTGATTTCAGC
CTGCCCCAAGTGGAACCAGTTGACCTCTCCTTTCACAAGCCCAAGGCTCCTCTCCAGCCT
GCTAGCATGCTACAAGCTCCAATACGTCCCCCCAAGCCACAGTCTTCTCCCCAGACCCTT
GTGGTGTCCACGTCAACATCTGACATGAGCACTTCAGCAAACATTCCTACTGTTCTGACC
CCAGGCTCTGTCCTGACCTCCTCTCAGAGCACTGGTAGCCAGCAGATCTTACATGTCATT
CACACTATCCCCTCAGTCAGTCTGCCAAATAAGATGGGTGGCCTGAAGACCATCCCAGTG
GTAGTGCAGTCTCTGCCCATGGTGTATACTACTTTGCCTGCAGATGGGGGCCCTGCAGCC
ATTACAGTCCCACTCATTGGAGGAGATGGTAAAAATGCTGGATCAGTGAAAGTTGACCCC
ACCTCCATGTCTCCACTGGAAATTCCAAGTGACAGTGAGGAGAGTACAATTGAGAGTGGA
TCCTCAGCCTTGCAGAGTCTGCAGGGACTACAGCAAGAACCAGCAGCAATGGCCCAAATG
CAGGGAGAAGAGTCGCTTGACTTGAAGAGAAGACGGATTCACCAATGTGACTTTGCAGGA
TGCAGCAAAGTGTACACCAAAAGCTCTCACCTGAAAGCTCACCGCAGAATCCATACAGGA
GAGAAGCCTTATAAATGCACCTGGGATGGCTGCTCCTGGAAATTTGCTCGCTCAGATGAG
CTCACTCGCCATTTCCGCAAGCACACAGGCATCAAGCCTTTTCGGTGCACAGACTGCAAC
CGCAGCTTTTCTCGTTCTGACCACCTGTCCCTGCATCGCCGTCGCCATGACACCATG

ORF Protein

Cloned ORF protein sequence for pFN21AE0926 Download

>KIEE0926  359 aa
MVDMDKLINNLEVQLNSEGGSMQVFKQVTASVRNRDPPEIEYRSNMTSPTLLDANPMENP
ALFNDIKIEPPEELLASDFSLPQVEPVDLSFHKPKAPLQPASMLQAPIRPPKPQSSPQTL
VVSTSTSDMSTSANIPTVLTPGSVLTSSQSTGSQQILHVIHTIPSVSLPNKMGGLKTIPV
VVQSLPMVYTTLPADGGPAAITVPLIGGDGKNAGSVKVDPTSMSPLEIPSDSEESTIESG
SSALQSLQGLQQEPAAMAQMQGEESLDLKRRRIHQCDFAGCSKVYTKSSHLKAHRRIHTG
EKPYKCTWDGCSWKFARSDELTRHFRKHTGIKPFRCTDCNRSFSRSDHLSLHRRRHDTM

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0926 Download

>pFN21AE0926  5782 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTCGATATGGATAAACTCATAAACAACTTGGA
GGTCCAACTTAATTCAGAAGGTGGCTCAATGCAGGTATTCAAGCAGGTCACTGCTTCTGT
TCGGAACAGAGATCCCCCTGAGATAGAATACAGAAGTAATATGACTTCTCCAACACTCCT
GGATGCCAACCCCATGGAGAACCCAGCACTGTTTAATGACATCAAGATTGAGCCCCCAGA
AGAACTTTTGGCTAGTGATTTCAGCCTGCCCCAAGTGGAACCAGTTGACCTCTCCTTTCA
CAAGCCCAAGGCTCCTCTCCAGCCTGCTAGCATGCTACAAGCTCCAATACGTCCCCCCAA
GCCACAGTCTTCTCCCCAGACCCTTGTGGTGTCCACGTCAACATCTGACATGAGCACTTC
AGCAAACATTCCTACTGTTCTGACCCCAGGCTCTGTCCTGACCTCCTCTCAGAGCACTGG
TAGCCAGCAGATCTTACATGTCATTCACACTATCCCCTCAGTCAGTCTGCCAAATAAGAT
GGGTGGCCTGAAGACCATCCCAGTGGTAGTGCAGTCTCTGCCCATGGTGTATACTACTTT
GCCTGCAGATGGGGGCCCTGCAGCCATTACAGTCCCACTCATTGGAGGAGATGGTAAAAA
TGCTGGATCAGTGAAAGTTGACCCCACCTCCATGTCTCCACTGGAAATTCCAAGTGACAG
TGAGGAGAGTACAATTGAGAGTGGATCCTCAGCCTTGCAGAGTCTGCAGGGACTACAGCA
AGAACCAGCAGCAATGGCCCAAATGCAGGGAGAAGAGTCGCTTGACTTGAAGAGAAGACG
GATTCACCAATGTGACTTTGCAGGATGCAGCAAAGTGTACACCAAAAGCTCTCACCTGAA
AGCTCACCGCAGAATCCATACAGGAGAGAAGCCTTATAAATGCACCTGGGATGGCTGCTC
CTGGAAATTTGCTCGCTCAGATGAGCTCACTCGCCATTTCCGCAAGCACACAGGCATCAA
GCCTTTTCGGTGCACAGACTGCAACCGCAGCTTTTCTCGTTCTGACCACCTGTCCCTGCA
TCGCCGTCGCCATGACACCATGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT