(Circular) (Six-base) MAPSORT of: pFN21AE0926.seq Check: 765 from: 1 to: 5782 pFN21AE0926 5782 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2570 3125 1937 Size: 633 555 4594 Fragments arranged by size: 4594 633 555 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2382 2549 3845 5229 5497 1632 Size: 750 167 1296 1384 268 1917 Fragments arranged by size: 1917 1384 1296 750 268 167 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 2476 4681 5658 1203 Size: 1273 2205 977 1327 Fragments arranged by size: 2205 1327 1273 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 3049 4511 3049 Size: 1462 4320 AleI CACnn'nnGTG Cuts at: 1166 2829 1166 Size: 1663 4119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2106 1184 Size: 922 4860 AlwNI CAG_nnn'CTG Cuts at: 2432 2557 2743 3525 5097 2432 Size: 125 186 782 1572 3117 Fragments arranged by size: 3117 1572 782 186 125 ApaI G_GGCC'C Cuts at: 2597 2597 Size: 5782 ApaLI G'TGCA_C Cuts at: 3010 3839 4995 3010 Size: 829 1156 3797 Fragments arranged by size: 3797 1156 829 ApoI r'AATT_y Cuts at: 1493 2685 2945 3091 3317 3469 5578 1493 Size: 1192 260 146 226 152 2109 1697 Fragments arranged by size: 2109 1697 1192 260 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 3107 1870 Size: 73 1164 4545 Fragments arranged by size: 4545 1164 73 BamHI G'GATC_C Cuts at: 2723 3112 3479 2723 Size: 389 367 5026 Fragments arranged by size: 5026 389 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3103 4558 618 Size: 325 217 627 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 325 217 BanII G_rGCy'C Cuts at: 1391 2214 2597 2967 3101 1391 Size: 823 383 370 134 4072 Fragments arranged by size: 4072 823 383 370 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2340 2537 2821 928 Size: 971 441 197 284 3889 Fragments arranged by size: 3889 971 441 284 197 BbvCI CC'TCA_GC Cuts at: 2728 2728 Size: 5782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3047 3081 3973 4007 1903 Size: 34 1110 34 892 34 3678 Fragments arranged by size: 3678 1110 892 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4393 136 Size: 107 122 71 631 605 2721 1525 Fragments arranged by size: 2721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2469 5777 2469 Size: 3308 2474 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 2597 3014 3843 4999 1790 Size: 807 417 829 1156 2573 Fragments arranged by size: 2573 1156 829 807 417 BmgBI CAC'GTC Cuts at: 1318 2376 1318 Size: 1058 4724 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2534 3513 4471 Size: 416 53 83 399 24 1117 979 958 Cuts at: 4471 442 Size: 1753 Fragments arranged by size: 1753 1117 979 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2310 1056 Size: 1254 4528 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 4442 1239 Size: 1043 2160 2579 Fragments arranged by size: 2579 2160 1043 Bpu10I CC'TnA_GC Cuts at: 2728 2728 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2651 2681 1178 Size: 30 165 30 1248 30 4279 Fragments arranged by size: 4279 1248 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2283 2434 2640 2717 1186 Size: 1097 151 206 77 4251 Fragments arranged by size: 4251 1097 206 151 77 BseYI C'CCAG_C Cuts at: 2182 4985 5481 2182 Size: 2803 496 2483 Fragments arranged by size: 2803 2483 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2570 1163 Size: 573 834 4375 Fragments arranged by size: 4375 834 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2396 2456 2967 3014 3843 3928 4999 2396 Size: 60 511 47 829 85 1071 3179 Fragments arranged by size: 3179 1071 829 511 85 60 47 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2396 2456 2597 2967 3014 3101 Size: 399 424 182 60 141 370 47 87 Cuts at: 3101 3843 3928 4999 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 399 370 182 141 87 85 60 47 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2062 2074 3137 844 Size: 1218 12 1063 3489 Fragments arranged by size: 3489 1218 1063 12 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2778 4277 4451 65 Size: 1183 1530 1499 174 1396 Fragments arranged by size: 1530 1499 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 2856 96 Size: 2760 3022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 2557 2743 3018 794 Size: 1763 186 275 3558 Fragments arranged by size: 3558 1763 275 186 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2173 2542 2173 Size: 369 5413 BstYI r'GATC_y Cuts at: 1972 2110 2469 2723 3112 3479 3864 3881 5333 Size: 138 359 254 389 367 385 17 1452 Cuts at: 5333 5419 5431 5777 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 389 385 367 359 346 254 138 86 17 12 BstZ17I GTA'TAC Cuts at: 2571 2571 Size: 5782 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2562 3078 513 Size: 552 939 168 390 516 3217 Fragments arranged by size: 3217 939 552 516 390 168 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3044 487 Size: 683 56 1818 3225 Fragments arranged by size: 3225 1818 683 56 BtsI GCAGTG_nn' Cuts at: 2088 3298 4092 4112 2088 Size: 1210 794 20 3758 Fragments arranged by size: 3758 1210 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747 Size: 54 1425 50 275 18 1399 889 1629 Cuts at: 5747 8 Size: 43 Fragments arranged by size: 1629 1425 1399 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2787 2804 3712 1353 Size: 1434 17 908 3423 Fragments arranged by size: 3423 1434 908 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2965 2965 Size: 5782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 2382 2549 3845 4442 5229 5497 Size: 393 650 100 167 1296 597 787 268 Cuts at: 5497 1239 Size: 1524 Fragments arranged by size: 1524 1296 787 650 597 393 268 167 100 EcoO109I rG'GnC_Cy Cuts at: 2593 2594 2593 Size: 1 5781 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2816 2816 Size: 5782 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 4929 1678 Size: 3251 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2431 2463 2651 2683 2708 Size: 32 135 32 859 32 188 32 25 Cuts at: 2708 2740 2921 2953 3035 3067 4423 4455 4497 Size: 32 181 32 82 32 1356 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 1356 859 188 181 135 82 42 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 2266 2379 2659 3126 3372 669 Size: 988 281 328 113 280 467 246 3079 Fragments arranged by size: 3079 988 467 328 281 280 246 113 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 2712 3381 2712 Size: 669 5113 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2068 2174 4896 5080 1305 Size: 763 106 2722 184 2007 Fragments arranged by size: 2722 2007 763 184 106 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 2669 2829 3740 4099 4258 518 Size: 648 1374 129 160 911 359 159 2042 Fragments arranged by size: 2042 1374 911 648 359 160 159 129 MspA1I CmG'CkG Cuts at: 1606 3198 3875 5023 5268 1606 Size: 1592 677 1148 245 2120 Fragments arranged by size: 2120 1592 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2172 2562 3078 513 Size: 552 939 168 390 516 3217 Fragments arranged by size: 3217 939 552 516 390 168 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 2306 1052 Size: 1254 4528 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2314 2480 3140 4685 1132 Size: 97 1085 166 660 1545 2229 Fragments arranged by size: 2229 1545 1085 660 166 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 2476 4681 2476 Size: 2205 3577 PflMI CCAn_nnn'nTGG Cuts at: 1108 2621 1108 Size: 1513 4269 PfoI T'CCnGG_A Cuts at: 2157 2939 2157 Size: 782 5000 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 2916 3352 2916 Size: 436 5346 PspOMI G'GGCC_C Cuts at: 2593 2593 Size: 5782 PstI C_TGCA'G Cuts at: 830 2587 2602 2748 3134 830 Size: 1757 15 146 386 3478 Fragments arranged by size: 3478 1757 386 146 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 SacI G_AGCT'C Cuts at: 2967 2967 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2583 2598 2744 2753 3130 4265 4946 Size: 219 1538 15 146 9 377 1135 681 Cuts at: 4946 5137 826 Size: 191 1471 Fragments arranged by size: 1538 1471 1135 681 377 219 191 146 15 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 2314 3140 1132 Size: 1182 826 3774 Fragments arranged by size: 3774 1182 826 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2004 2172 2286 2562 3078 3221 513 Size: 552 939 168 114 276 516 143 3074 Fragments arranged by size: 3074 939 552 516 276 168 143 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2510 3809 3826 3979 4164 4609 325 Size: 1255 930 1299 17 153 185 445 1498 Fragments arranged by size: 1498 1299 1255 930 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2708 2856 4028 Size: 274 80 33 51 1443 731 148 1172 Cuts at: 4028 5740 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1172 731 274 148 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2677 2011 Size: 666 5116 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI