NIM1K ORF clone pFN21AE0762

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Halotag ORF Clone pFN21AE0762

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Clone info.

Species

human

Clone Name

pFN21AE0762 Sequence (with vector) Restriction Map

Product ID

FHC11682

Flexi Clone PID

FXC11682

ORF Sequence

DNA sequence (1308 bp)
Protein sequence (436 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529175]

Gene

KIEE0762

Symbol

NIM1K

Alias : NIM1

Description

NIM1 serine/threonine protein kinase, transcript variant 1

Original Clone ID

cs02262

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1308 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 436 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3943.1 1.6e-96 100.0 NIM1K CCDS8347.1 1.9e-26 40.5 SIK2 CCDS8379.2 1.2e-25 40.6 SIK3 CCDS33575.1 1.8e-25 39.6 SIK1 CCDS82645.1 1.8e-25 39.6 LOC102724428 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017442 74 325 PF00069 Serine/threonine-protein kinase-like domain HMMSmart IPR002290 74 325 SM00220 Serine/threonine-protein kinase domain IPR020635 74 324 SM00219 Tyrosine-protein kinase ProfileScan IPR000719 74 325 PS50011 Protein kinase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 248 IGIYVDIWALGVLLYFMVTGTM 269 PRIMARY 22 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0762 Download

>KIEE0762  1308 bp
ATGACTGCAGTGTATATGAATGGAGGTGGCCTGGTGAACCCCCATTATGCCCGGTGGGAT
CGGCGCGACAGTGTAGAAAGTGGCTGTCAGACCGAGAGTAGCAAGGAGGGTGAGGAGGGA
CAGCCCCGCCAGCTGACGCCCTTCGAGAAACTGACACAGGACATGTCCCAGGATGAGAAG
GTGGTGAGGGAGATCACGCTGGGGAAACGGATAGGCTTCTACCGAATTCGAGGGGAAATC
GGAAGTGGAAACTTCTCCCAAGTGAAGCTTGGGATTCACTCCCTAACCAAAGAAAAGGTG
GCCATTAAGATCCTGGACAAGACCAAGTTAGACCAGAAAACCCAGAGGCTACTATCCCGA
GAAATCTCCAGCATGGAAAAGCTGCACCATCCCAACATCATCCGCCTTTACGAAGTGGTG
GAGACCCTATCCAAGCTGCACTTGGTGATGGAGTATGCAGGGGGTGGGGAGCTCTTCGGA
AAAATTAGCACTGAGGGGAAGCTCTCTGAACCAGAAAGCAAGCTCATCTTCTCCCAGATT
GTGTCTGCCGTGAAGCACATGCATGAAAACCAAATTATTCATAGAGATCTGAAAGCAGAA
AATGTATTCTATACCAGTAATACTTGTGTGAAGGTGGGCGATTTTGGATTCAGCACAGTA
AGCAAAAAAGGTGAAATGCTGAACACTTTCTGTGGGTCTCCTCCCTACGCTGCGCCTGAA
CTCTTCCGGGACGAGCACTACATCGGCATTTACGTGGATATCTGGGCCTTGGGGGTGCTT
TTGTACTTCATGGTGACTGGCACCATGCCATTTCGGGCAGAAACCGTGGCCAAACTAAAA
AAGAGCATCCTCGAGGGCACATACAGTGTACCGCCGCACGTGTCAGAGCCCTGCCACCGA
CTCATCCGAGGAGTCCTTCAGCAGATCCCCACGGAGAGGTACGGAATCGACTGCATCATG
AATGATGAATGGATGCAAGGGGTGCCATACCCTACACCTTTGGAACCTTTCCAACTGGAT
CCCAAACATTTGTCGGAAACCAGCACTCTCAAGGAAGAAGAAAATGAGGTCAAAAGCACT
TTAGAACATTTGGGCATTACAGAAGAGCATATTCGAAATAACCAAGGGAGAGATGCTCGC
AGCTCAATCACAGGGGTCTATAGAATTATTTTACATAGAGTCCAAAGGAAGAAGGCTTTG
GAAAGTGTCCCAGTCATGATGCTACCAGACCCTAAAGAAAGAGACCTCAAAAAAGGGTCC
CGTGTCTACAGAGGGATAAGACACACATCCAAATTTTGCTCGATTTTA

ORF Protein

Cloned ORF protein sequence for pFN21AE0762 Download

>KIEE0762  436 aa
MTAVYMNGGGLVNPHYARWDRRDSVESGCQTESSKEGEEGQPRQLTPFEKLTQDMSQDEK
VVREITLGKRIGFYRIRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSR
EISSMEKLHHPNIIRLYEVVETLSKLHLVMEYAGGGELFGKISTEGKLSEPESKLIFSQI
VSAVKHMHENQIIHRDLKAENVFYTSNTCVKVGDFGFSTVSKKGEMLNTFCGSPPYAAPE
LFRDEHYIGIYVDIWALGVLLYFMVTGTMPFRAETVAKLKKSILEGTYSVPPHVSEPCHR
LIRGVLQQIPTERYGIDCIMNDEWMQGVPYPTPLEPFQLDPKHLSETSTLKEEENEVKST
LEHLGITEEHIRNNQGRDARSSITGVYRIILHRVQRKKALESVPVMMLPDPKERDLKKGS
RVYRGIRHTSKFCSIL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0762 Download

>pFN21AE0762  6013 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACTGCAGTGTATATGAATGGAGGTGGCCTGGT
GAACCCCCATTATGCCCGGTGGGATCGGCGCGACAGTGTAGAAAGTGGCTGTCAGACCGA
GAGTAGCAAGGAGGGTGAGGAGGGACAGCCCCGCCAGCTGACGCCCTTCGAGAAACTGAC
ACAGGACATGTCCCAGGATGAGAAGGTGGTGAGGGAGATCACGCTGGGGAAACGGATAGG
CTTCTACCGAATTCGAGGGGAAATCGGAAGTGGAAACTTCTCCCAAGTGAAGCTTGGGAT
TCACTCCCTAACCAAAGAAAAGGTGGCCATTAAGATCCTGGACAAGACCAAGTTAGACCA
GAAAACCCAGAGGCTACTATCCCGAGAAATCTCCAGCATGGAAAAGCTGCACCATCCCAA
CATCATCCGCCTTTACGAAGTGGTGGAGACCCTATCCAAGCTGCACTTGGTGATGGAGTA
TGCAGGGGGTGGGGAGCTCTTCGGAAAAATTAGCACTGAGGGGAAGCTCTCTGAACCAGA
AAGCAAGCTCATCTTCTCCCAGATTGTGTCTGCCGTGAAGCACATGCATGAAAACCAAAT
TATTCATAGAGATCTGAAAGCAGAAAATGTATTCTATACCAGTAATACTTGTGTGAAGGT
GGGCGATTTTGGATTCAGCACAGTAAGCAAAAAAGGTGAAATGCTGAACACTTTCTGTGG
GTCTCCTCCCTACGCTGCGCCTGAACTCTTCCGGGACGAGCACTACATCGGCATTTACGT
GGATATCTGGGCCTTGGGGGTGCTTTTGTACTTCATGGTGACTGGCACCATGCCATTTCG
GGCAGAAACCGTGGCCAAACTAAAAAAGAGCATCCTCGAGGGCACATACAGTGTACCGCC
GCACGTGTCAGAGCCCTGCCACCGACTCATCCGAGGAGTCCTTCAGCAGATCCCCACGGA
GAGGTACGGAATCGACTGCATCATGAATGATGAATGGATGCAAGGGGTGCCATACCCTAC
ACCTTTGGAACCTTTCCAACTGGATCCCAAACATTTGTCGGAAACCAGCACTCTCAAGGA
AGAAGAAAATGAGGTCAAAAGCACTTTAGAACATTTGGGCATTACAGAAGAGCATATTCG
AAATAACCAAGGGAGAGATGCTCGCAGCTCAATCACAGGGGTCTATAGAATTATTTTACA
TAGAGTCCAAAGGAAGAAGGCTTTGGAAAGTGTCCCAGTCATGATGCTACCAGACCCTAA
AGAAAGAGACCTCAAAAAAGGGTCCCGTGTCTACAGAGGGATAAGACACACATCCAAATT
TTGCTCGATTTTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT