(Circular) (Six-base) MAPSORT of: pFN21AE0762.seq Check: 8678 from: 1 to: 6013 pFN21AE0762 6013 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3270 3356 1937 Size: 1333 86 4594 Fragments arranged by size: 4594 1333 86 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2906 4076 5460 5728 1632 Size: 1274 1170 1384 268 1917 Fragments arranged by size: 1917 1384 1274 1170 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 2166 2883 4912 5889 1203 Size: 963 717 2029 977 1327 Fragments arranged by size: 2029 1327 977 963 717 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AlwNI CAG_nnn'CTG Cuts at: 3756 5328 3756 Size: 1572 4441 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 2229 3296 3322 3548 3700 5809 1493 Size: 736 1067 26 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 736 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 2361 2855 3338 1870 Size: 73 418 494 483 4545 Fragments arranged by size: 4545 494 483 418 73 BamHI G'GATC_C Cuts at: 3022 3343 3710 3022 Size: 321 367 5325 Fragments arranged by size: 5325 367 321 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2804 2986 3334 4789 618 Size: 325 217 627 1017 182 348 1455 1842 Fragments arranged by size: 1842 1455 1017 627 348 325 217 182 BanII G_rGCy'C Cuts at: 1391 2478 2895 3332 1391 Size: 1087 417 437 4072 Fragments arranged by size: 4072 1087 437 417 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5042 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BfrBI ATG'CAT Cuts at: 2566 2566 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4624 136 Size: 107 122 71 631 605 2952 1525 Fragments arranged by size: 2952 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2590 6008 2590 Size: 3418 2595 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 2864 4074 5230 1790 Size: 1074 1210 1156 2573 Fragments arranged by size: 2573 1210 1156 1074 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6013 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3209 3744 4702 Size: 416 53 83 399 24 1792 535 958 Cuts at: 4702 442 Size: 1753 Fragments arranged by size: 1792 1753 958 535 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3156 3156 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2356 4673 1239 Size: 1117 2317 2579 Fragments arranged by size: 2579 2317 1117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3038 4138 5003 5301 3038 Size: 1100 865 298 3750 Fragments arranged by size: 3750 1100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2420 2706 3240 4676 882 Size: 617 921 286 534 1436 2219 Fragments arranged by size: 2219 1436 921 617 534 286 BsaAI yAC'GTr Cuts at: 493 1204 2758 2884 3804 493 Size: 711 1554 126 920 2702 Fragments arranged by size: 2702 1554 920 711 126 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 3820 4202 4837 Size: 53 83 186 936 608 1679 382 635 Cuts at: 4837 275 Size: 1451 Fragments arranged by size: 1679 1451 936 635 608 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 4844 5118 5265 5676 4446 Size: 398 274 147 411 4783 Fragments arranged by size: 4783 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5788 Fragments arranged by size: 5788 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2694 2928 1186 Size: 946 562 234 4271 Fragments arranged by size: 4271 946 562 234 BseYI C'CCAG_C Cuts at: 2203 5216 5712 2203 Size: 3013 496 2504 Fragments arranged by size: 3013 2504 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 2426 1163 Size: 573 636 54 4750 Fragments arranged by size: 4750 636 573 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3463 4224 5252 1540 Size: 275 186 1462 761 1028 2301 Fragments arranged by size: 2301 1462 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2478 2742 4074 4159 5230 2478 Size: 264 1332 85 1071 3261 Fragments arranged by size: 3261 1332 1071 264 85 BsmI GAATG_Cn' Cuts at: 3524 3617 3524 Size: 93 5920 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2478 2742 2864 2895 3332 4074 4159 Size: 399 688 264 122 31 437 742 85 Cuts at: 4159 5230 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 688 437 399 264 122 85 31 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 2961 3219 3902 5632 2961 Size: 258 683 1730 3342 Fragments arranged by size: 3342 1730 683 258 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3368 844 Size: 2524 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3900 1394 Size: 429 2077 3507 Fragments arranged by size: 3507 2077 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4508 4682 65 Size: 1183 3260 174 1396 Fragments arranged by size: 3260 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4657 4844 1700 Size: 110 2847 187 2869 Fragments arranged by size: 2869 2847 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 2986 794 Size: 2192 3821 BstBI TT'CG_AA Cuts at: 3118 4817 3118 Size: 1699 4314 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstYI r'GATC_y Cuts at: 1972 2313 2590 2928 3022 3343 3710 4095 4112 Size: 341 277 338 94 321 367 385 17 Cuts at: 4112 5564 5650 5662 6008 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 341 338 321 277 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2829 2934 513 Size: 552 1764 105 3592 Fragments arranged by size: 3592 1764 552 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 2019 3529 4323 4343 2019 Size: 1510 794 20 3689 Fragments arranged by size: 3689 1510 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DraIII CAC_nnn'GTG Cuts at: 2449 2449 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2304 2832 3460 Size: 54 1425 50 275 18 474 528 628 Cuts at: 3460 4349 5978 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 628 528 474 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2483 2732 3102 3943 1353 Size: 1130 249 370 841 3423 Fragments arranged by size: 3423 1130 841 370 249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2396 4600 4974 5120 387 Size: 1064 138 807 2204 374 146 1280 Fragments arranged by size: 2204 1280 1064 807 374 146 138 EcoICRI GAG'CTC Cuts at: 2476 2476 Size: 6013 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2356 2906 4076 4673 5460 5728 1239 Size: 393 724 550 1170 597 787 268 1524 Fragments arranged by size: 1524 1170 787 724 597 550 393 268 EcoO109I rG'GnC_Cy Cuts at: 3261 3261 Size: 6013 EcoRI G'AATT_C Cuts at: 2229 3322 3700 2229 Size: 1093 378 4542 Fragments arranged by size: 4542 1093 378 EcoRV GAT'ATC Cuts at: 2764 2764 Size: 6013 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2288 2461 2548 2288 Size: 173 87 5753 Fragments arranged by size: 5753 173 87 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 5160 1678 Size: 3482 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2226 2258 2417 2449 4654 Size: 32 135 32 654 32 159 32 2205 Cuts at: 4654 4686 4728 4760 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2205 654 159 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 2270 748 Size: 1522 4491 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3018 3040 5127 5311 1305 Size: 1713 22 2087 184 2007 Fragments arranged by size: 2087 2007 1713 184 22 MscI TGG'CCA Cuts at: 10 64 1489 1832 2306 2834 10 Size: 54 1425 343 474 528 3189 Fragments arranged by size: 3189 1425 528 474 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2565 2756 3971 4330 4489 518 Size: 648 1399 191 1215 359 159 2042 Fragments arranged by size: 2042 1399 1215 648 359 191 159 MspA1I CmG'CkG Cuts at: 1606 2137 3429 4106 5254 5499 1606 Size: 531 1292 677 1148 245 2120 Fragments arranged by size: 2120 1292 1148 677 531 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5461 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NsiI A_TGCA'T Cuts at: 2568 2568 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 2170 2566 3371 4916 1132 Size: 97 941 396 805 1545 2229 Fragments arranged by size: 2229 1545 941 805 396 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6013 PciI A'CATG_T Cuts at: 2166 4912 2166 Size: 2746 3267 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6013 PfoI T'CCnGG_A Cuts at: 2316 2730 2316 Size: 414 5599 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PmlI CAC'GTG Cuts at: 2884 2884 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5595 5627 4082 Size: 32 1481 32 4468 Fragments arranged by size: 4468 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3261 3261 Size: 6013 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PspXI vC'TCGA_Gb Cuts at: 2855 2855 Size: 6013 PstI C_TGCA'G Cuts at: 830 2014 3365 830 Size: 1184 1351 3478 Fragments arranged by size: 3478 1351 1184 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 PvuII CAG'CTG Cuts at: 2137 2137 Size: 6013 SacI G_AGCT'C Cuts at: 2478 2478 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SanDI GG'GwC_CC Cuts at: 3261 3261 Size: 6013 SapI GCTCTTCn'nnn_ Cuts at: 2483 3102 2483 Size: 619 5394 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SfcI C'TryA_G Cuts at: 826 1045 2010 3163 3271 3361 4496 5177 5368 Size: 219 965 1153 108 90 1135 681 191 Cuts at: 5368 826 Size: 1471 Fragments arranged by size: 1471 1153 1135 965 681 219 191 108 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 3340 1872 Size: 1468 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2855 3053 4117 5018 5280 820 Size: 197 926 912 198 1064 901 262 1553 Fragments arranged by size: 1553 1064 926 912 901 262 198 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6013 StyI C'CwwG_G Cuts at: 513 1065 2772 3127 3452 513 Size: 552 1707 355 325 3074 Fragments arranged by size: 3074 1707 552 355 325 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2111 4040 4057 4210 4395 4840 325 Size: 1255 531 1929 17 153 185 445 1498 Fragments arranged by size: 1929 1498 1255 531 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2787 4259 5971 Size: 274 80 33 51 1443 810 1472 1712 Cuts at: 5971 96 Size: 138 Fragments arranged by size: 1712 1472 1443 810 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2270 2299 2314 2443 2270 Size: 29 15 129 5840 Fragments arranged by size: 5840 129 29 15 XhoI C'TCGA_G Cuts at: 1943 2855 1943 Size: 912 5101 XmaI C'CCGG_G Cuts at: 1870 3338 1870 Size: 1468 4545 XmnI GAAnn'nnTTC Cuts at: 2690 4142 2690 Size: 1452 4561 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI