GJC3 ORF clone pFN21AE0579

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0579

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Clone info.

Species

human

Clone Name

pFN21AE0579 Sequence (with vector) Restriction Map

Product ID

FHC11179

Flexi Clone PID

FXC11179

ORF Sequence

DNA sequence (837 bp)
Protein sequence (279 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528858]

Gene

KIEE0579

Symbol

GJC3

Alias : CX29, CX30.2, CX31.3, GJE1

Description

gap junction protein, gamma 3, 30.2kDa

Original Clone ID

oc01279

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 837 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 279 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34697.1 8.3e-129 100.0 GJC3 CCDS9290.1 2.2e-26 36.8 GJB2 CCDS14408.1 1.1e-25 36.0 GJB1 CCDS9291.1 4.7e-25 28.8 GJB6 CCDS58547.1 7.1e-25 32.6 GJD3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000500 20 44 PR00206 Connexin IPR000500 51 73 PR00206 Connexin IPR000500 76 96 PR00206 Connexin IPR000500 133 159 PR00206 Connexin IPR000500 169 189 PR00206 Connexin IPR000500 190 213 PR00206 Connexin HMMPfam IPR013092 5 99 PF00029 Connexin IPR019570 147 213 PF10582 Gap junction protein HMMSmart IPR013092 42 75 SM00037 Connexin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 19 PVGRLLLPVLLGFRLVLLAASG 40 SECONDARY 22 2 75 RFWVFQVILVAVPSALYMGFTLY 97 PRIMARY 23 3 128 AGSLRLLWAYVAQLGARLVLEGA 150 SECONDARY 23 4 192 LKTMFGVSGFCLLFTFLELVLLG 214 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_853516 279 611925 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0579 Download

>KIEE0579  837 bp
ATGTGTGGCAGGTTCCTGCGGCGGCTGCTGGCGGAGGAGAGCCGGCGCTCCACCCCCGTG
GGGCGCCTCTTGCTTCCCGTGCTCCTGGGATTCCGCCTTGTGCTGCTGGCTGCCAGTGGG
CCTGGAGTCTATGGTGATGAGCAGAGTGAATTCGTGTGTCACACCCAGCAGCCGGGCTGC
AAGGCTGCCTGCTTCGATGCCTTCCACCCCCTCTCCCCGCTGCGTTTCTGGGTCTTCCAG
GTCATCTTGGTGGCTGTACCCAGCGCCCTCTATATGGGTTTCACTCTGTATCACGTGATC
TGGCACTGGGAATTATCAGGAAAGGGGAAGGAGGAGGAGACCCTGATCCAGGGACGGGAG
GGCAACACAGATGTCCCAGGGGCTGGAAGCCTCAGGCTGCTCTGGGCTTATGTGGCTCAG
CTGGGGGCTCGGCTTGTCCTGGAGGGGGCAGCCCTGGGGTTGCAGTACCACCTGTATGGG
TTCCAGATGCCCAGCTCCTTTGCATGTCGCCGAGAACCTTGCCTTGGTAGTATAACCTGC
AATCTGTCCCGCCCCTCTGAGAAGACCATTTTCCTAAAGACCATGTTTGGAGTCAGCGGT
TTCTGTCTCTTGTTTACTTTTTTGGAGCTTGTGCTTCTGGGTTTGGGGAGATGGTGGAGG
ACCTGGAAGCACAAATCTTCCTCTTCTAAATACTTCCTAACTTCAGAGAGCACCAGAAGA
CACAAGAAAGCAACCGATAGCCTCCCAGTGGTGGAAACCAAAGAGCAATTTCAAGAAGCA
GTTCCAGGAAGAAGCTTAGCCCAGGAAAAACAAAGACCAGTTGGACCCAGAGATGCC

ORF Protein

Cloned ORF protein sequence for pFN21AE0579 Download

>KIEE0579  279 aa
MCGRFLRRLLAEESRRSTPVGRLLLPVLLGFRLVLLAASGPGVYGDEQSEFVCHTQQPGC
KAACFDAFHPLSPLRFWVFQVILVAVPSALYMGFTLYHVIWHWELSGKGKEEETLIQGRE
GNTDVPGAGSLRLLWAYVAQLGARLVLEGAALGLQYHLYGFQMPSSFACRREPCLGSITC
NLSRPSEKTIFLKTMFGVSGFCLLFTFLELVLLGLGRWWRTWKHKSSSSKYFLTSESTRR
HKKATDSLPVVETKEQFQEAVPGRSLAQEKQRPVGPRDA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0579 Download

>pFN21AE0579  5542 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGTGGCAGGTTCCTGCGGCGGCTGCTGGCGGA
GGAGAGCCGGCGCTCCACCCCCGTGGGGCGCCTCTTGCTTCCCGTGCTCCTGGGATTCCG
CCTTGTGCTGCTGGCTGCCAGTGGGCCTGGAGTCTATGGTGATGAGCAGAGTGAATTCGT
GTGTCACACCCAGCAGCCGGGCTGCAAGGCTGCCTGCTTCGATGCCTTCCACCCCCTCTC
CCCGCTGCGTTTCTGGGTCTTCCAGGTCATCTTGGTGGCTGTACCCAGCGCCCTCTATAT
GGGTTTCACTCTGTATCACGTGATCTGGCACTGGGAATTATCAGGAAAGGGGAAGGAGGA
GGAGACCCTGATCCAGGGACGGGAGGGCAACACAGATGTCCCAGGGGCTGGAAGCCTCAG
GCTGCTCTGGGCTTATGTGGCTCAGCTGGGGGCTCGGCTTGTCCTGGAGGGGGCAGCCCT
GGGGTTGCAGTACCACCTGTATGGGTTCCAGATGCCCAGCTCCTTTGCATGTCGCCGAGA
ACCTTGCCTTGGTAGTATAACCTGCAATCTGTCCCGCCCCTCTGAGAAGACCATTTTCCT
AAAGACCATGTTTGGAGTCAGCGGTTTCTGTCTCTTGTTTACTTTTTTGGAGCTTGTGCT
TCTGGGTTTGGGGAGATGGTGGAGGACCTGGAAGCACAAATCTTCCTCTTCTAAATACTT
CCTAACTTCAGAGAGCACCAGAAGACACAAGAAAGCAACCGATAGCCTCCCAGTGGTGGA
AACCAAAGAGCAATTTCAAGAAGCAGTTCCAGGAAGAAGCTTAGCCCAGGAAAAACAAAG
ACCAGTTGGACCCAGAGATGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT