Restriction map for
pFN21AE0579
(Circular) (Six-base) MAPSORT of: pFN21AE0579.seq Check: 156 from: 1 to: 5542
pFN21AE0579 5542 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3352 278
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 1937
Size: 948 4594
Acc65I G'GTAC_C
Cuts at: 2863 2863
Size: 5542
AclI AA'CG_TT
Cuts at: 1563 3669 4042 1563
Size: 2106 373 3063
Fragments arranged by size:
3063 2106 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2691 3605 4989 5257 1632
Size: 1059 914 1384 268 1917
Fragments arranged by size:
1917 1384 1059 914 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5345
AflIII A'CryG_T
Cuts at: 1203 4441 5418 1203
Size: 3238 977 1327
Fragments arranged by size:
3238 1327 977
AgeI A'CCGG_T
Cuts at: 4373 4373
Size: 5542
AhdI GACnn_n'nnGTC
Cuts at: 4271 4271
Size: 5542
AleI CACnn'nnGTG
Cuts at: 1166 2060 1166
Size: 894 4648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2036 1184
Size: 852 4690
AlwNI CAG_nnn'CTG
Cuts at: 2387 3285 4857 2387
Size: 898 1572 3072
Fragments arranged by size:
3072 1572 898
ApaLI G'TGCA_C
Cuts at: 3599 4755 3599
Size: 1156 4386
ApoI r'AATT_y
Cuts at: 1493 2153 2851 3077 3229 5338 1493
Size: 660 698 226 152 2109 1697
Fragments arranged by size:
2109 1697 698 660 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4096 160
Size: 872 3064 1606
Fragments arranged by size:
3064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
AvaI C'yCGr_G
Cuts at: 1870 1943 2867 1870
Size: 73 924 4545
Fragments arranged by size:
4545 924 73
BamHI G'GATC_C
Cuts at: 2872 3239 2872
Size: 367 5175
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2067 2863 4318 618
Size: 325 217 627 280 796 1455 1842
Fragments arranged by size:
1842 1455 796 627 325 280 217
BanII G_rGCy'C
Cuts at: 1391 2434 2861 1391
Size: 1043 427 4072
Fragments arranged by size:
4072 1043 427
BbeI G_GCGC'C
Cuts at: 2071 2071
Size: 5542
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2230 2573 2728 928
Size: 971 331 343 155 3742
Fragments arranged by size:
3742 971 343 331 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2505 2539 3733 3767 1903
Size: 34 568 34 1194 34 3678
Fragments arranged by size:
3678 1194 568 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3434 4644 3434
Size: 1210 4332
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4153 136
Size: 107 122 71 631 605 2481 1525
Fragments arranged by size:
2481 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5537 5537
Size: 5542
BlpI GC'TnA_GC
Cuts at: 2421 2800 2959 2421
Size: 379 159 5004
Fragments arranged by size:
5004 379 159
Bme1580I G_kGCm'C
Cuts at: 1790 3603 4759 1790
Size: 1813 1156 2573
Fragments arranged by size:
2573 1813 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5542
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2320 2744 3273
Size: 416 53 83 399 24 903 424 529
Cuts at: 3273 4231 442
Size: 958 1753
Fragments arranged by size:
1753 958 903 529 424 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2148 2465 4202 1239
Size: 909 317 1737 2579
Fragments arranged by size:
2579 1737 909 317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3667 4532 4830 3667
Size: 865 298 4379
Fragments arranged by size:
4379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2336 4205 882
Size: 617 837 1869 2219
Fragments arranged by size:
2219 1869 837 617
BsaAI yAC'GTr
Cuts at: 493 1204 2299 3333 493
Size: 711 1095 1034 2702
Fragments arranged by size:
2702 1095 1034 711
BsaBI GATnn'nnATC
Cuts at: 5536 5536
Size: 5542
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2068 3349 3731 4366
Size: 53 83 186 936 535 1281 382 635
Cuts at: 4366 275
Size: 1451
Fragments arranged by size:
1451 1281 936 635 535 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3975 4373 4647 4794 5205 3975
Size: 398 274 147 411 4312
Fragments arranged by size:
4312 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 1178
Size: 30 165 30 798 30 4489
Fragments arranged by size:
4489 798 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2351 2354 1186
Size: 868 297 3 4374
Fragments arranged by size:
4374 868 297 3
BseYI C'CCAG_C
Cuts at: 2169 2264 2425 2495 4745 5241 2169
Size: 95 161 70 2250 496 2470
Fragments arranged by size:
2470 2250 496 161 95 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4969
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2992 3753 4781 1540
Size: 275 186 991 761 1028 2301
Fragments arranged by size:
2301 1028 991 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2717 3603 3688 4759 2088
Size: 629 886 85 1071 2871
Fragments arranged by size:
2871 1071 886 629 85
BsmI GAATG_Cn'
Cuts at: 3053 3146 3053
Size: 93 5449
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2434 2717 2861 3603 3688 4759
Size: 399 298 346 283 144 742 85 1071
Cuts at: 4759 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 346 298 283 144
85
BspEI T'CCGG_A
Cuts at: 5205 5205
Size: 5542
BspHI T'CATG_A
Cuts at: 3431 5161 3431
Size: 1730 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2004 2549 2897 844
Size: 1160 545 348 3489
Fragments arranged by size:
3489 1160 545 348
BsrBI CCG'CTC
Cuts at: 1394 1823 3429 1394
Size: 429 1606 3507
Fragments arranged by size:
3507 1606 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4037 4211 65
Size: 1183 2789 174 1396
Fragments arranged by size:
2789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 4186 4373 1700
Size: 110 236 2140 187 2869
Fragments arranged by size:
2869 2140 236 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5542
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5542
BssSI C'ACGA_G
Cuts at: 3602 4614 3602
Size: 1012 4530
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5542
BstBI TT'CG_AA
Cuts at: 4346 4346
Size: 5542
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5542
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2480 2062
Size: 418 5124
BstYI r'GATC_y
Cuts at: 1972 2872 3239 3624 3641 5093 5179 5191 5537
Size: 900 367 385 17 1452 86 12 346
Cuts at: 5537 1972
Size: 1977
Fragments arranged by size:
1977 1452 900 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2396 2396
Size: 5542
BtgI C'CryG_G
Cuts at: 513 1065 2061 513
Size: 552 996 3994
Fragments arranged by size:
3994 996 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4803
Fragments arranged by size:
4803 683 56
BtsI GCAGTG_nn'
Cuts at: 3058 3852 3872 3058
Size: 794 20 4728
Fragments arranged by size:
4728 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5507
DraI TTT'AAA
Cuts at: 2846 2846
Size: 5542
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4549 809
Size: 507 3233 1802
Fragments arranged by size:
3233 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2989 3878 5507
Size: 54 1425 50 275 18 1159 889 1629
Cuts at: 5507 8
Size: 43
Fragments arranged by size:
1629 1425 1159 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2989 1537
Size: 275 1177 4090
Fragments arranged by size:
4090 1177 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2692 3472 1353
Size: 1339 780 3423
Fragments arranged by size:
3423 1339 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 2087 4129 4503 4649 387
Size: 1064 138 462 36 2042 374 146 1280
Fragments arranged by size:
2042 1280 1064 462 374 146 138 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2465 2691 3605 4202 4989 5257
Size: 393 516 317 226 914 597 787 268
Cuts at: 5257 1239
Size: 1524
Fragments arranged by size:
1524 914 787 597 516 393 317 268
226
EcoNI CCTnn'n_nnAGG
Cuts at: 2351 2351
Size: 5542
EcoO109I rG'GnC_Cy
Cuts at: 2664 2664
Size: 5542
EcoRI G'AATT_C
Cuts at: 2153 2851 3229 2153
Size: 698 378 4466
Fragments arranged by size:
4466 698 378
FspI TGC'GCA
Cuts at: 4048 4048
Size: 5542
HaeII r_GCGC'y
Cuts at: 1678 2053 2071 2271 4689 1678
Size: 375 18 200 2418 2531
Fragments arranged by size:
2531 2418 375 200 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4183 4215 4257 4289 1373
Size: 32 135 32 2611 32 42 32 2626
Fragments arranged by size:
2626 2611 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2886 3132 669
Size: 988 281 948 246 3079
Fragments arranged by size:
3079 988 948 281 246
HindIII A'AGCT_T
Cuts at: 748 2797 748
Size: 2049 3493
HpaI GTT'AAC
Cuts at: 3132 3132
Size: 5542
KasI G'GCGC_C
Cuts at: 2067 2067
Size: 5542
KpnI G_GTAC'C
Cuts at: 2867 2867
Size: 5542
MfeI C'AATT_G
Cuts at: 3141 3141
Size: 5542
MluI A'CGCG_T
Cuts at: 5418 5418
Size: 5542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2806 4656 4840 1305
Size: 1501 1850 184 2007
Fragments arranged by size:
2007 1850 1501 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3720
Fragments arranged by size:
3720 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2252 2478 3500 3859 4018 518
Size: 648 894 192 226 1022 359 159 2042
Fragments arranged by size:
2042 1022 894 648 359 226 192 159
MspA1I CmG'CkG
Cuts at: 1606 2224 2425 2601 2958 3635 4783 5028 1606
Size: 618 201 176 357 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 618 357 245 201 176
NaeI GCC'GGC
Cuts at: 2048 2048
Size: 5542
NarI GG'CG_CC
Cuts at: 2068 2068
Size: 5542
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4990
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5542
NgoMIV G'CCGG_C
Cuts at: 2046 2046
Size: 5542
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5542
NotI GC'GGCC_GC
Cuts at: 2989 2989
Size: 5542
NspI r_CATG'y
Cuts at: 1132 1229 2511 2900 4445 1132
Size: 97 1282 389 1545 2229
Fragments arranged by size:
2229 1545 1282 389 97
PasI CC'CwG_GG
Cuts at: 1348 2381 2458 1348
Size: 1033 77 4432
Fragments arranged by size:
4432 1033 77
PciI A'CATG_T
Cuts at: 4441 4441
Size: 5542
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2387 1108
Size: 1279 4263
PfoI T'CCnGG_A
Cuts at: 2442 2788 2442
Size: 346 5196
PmeI GTTT'AAAC
Cuts at: 2846 2846
Size: 5542
PmlI CAC'GTG
Cuts at: 2299 2299
Size: 5542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 5124 5156 3611
Size: 32 1481 32 3997
Fragments arranged by size:
3997 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2664 2664
Size: 5542
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5542
PsiI TTA'TAA
Cuts at: 3112 3112
Size: 5542
PstI C_TGCA'G
Cuts at: 830 2894 830
Size: 2064 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5542
PvuII CAG'CTG
Cuts at: 2425 2425
Size: 5542
SalI G'TCGA_C
Cuts at: 1936 2884 1936
Size: 948 4594
SbfI CC_TGCA'GG
Cuts at: 2894 2894
Size: 5542
ScaI AGT'ACT
Cuts at: 3790 5502 3790
Size: 1712 3830
SfcI C'TryA_G
Cuts at: 826 1045 2890 4025 4706 4897 826
Size: 219 1845 1135 681 191 1471
Fragments arranged by size:
1845 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2069 2069
Size: 5542
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5542
SmaI CCC'GGG
Cuts at: 1872 2869 1872
Size: 997 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3646 4547 4809 820
Size: 197 926 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3333 493
Size: 2840 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5542
SphI G_CATG'C
Cuts at: 1132 2900 1132
Size: 1768 3774
SspI AAT'ATT
Cuts at: 5 52 3466 5
Size: 47 3414 2081
Fragments arranged by size:
3414 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5542
StyI C'CwwG_G
Cuts at: 513 1065 2527 2981 513
Size: 552 1462 454 3074
Fragments arranged by size:
3074 1462 552 454
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 3569 3586 3739 3924 4369 325
Size: 1255 603 1386 17 153 185 445 1498
Fragments arranged by size:
1498 1386 1255 603 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3788 5500 96
Size: 274 80 33 51 1443 1811 1712 138
Fragments arranged by size:
1811 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 2666 2698 5502 5534 2201
Size: 32 433 32 2804 32 2209
Fragments arranged by size:
2804 2209 433 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5542
XbaI T'CTAG_A
Cuts at: 2878 2878
Size: 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5542
XmaI C'CCGG_G
Cuts at: 1870 2867 1870
Size: 997 4545
XmnI GAAnn'nnTTC
Cuts at: 2784 3671 2784
Size: 887 4655
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3350 276
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI