KRT28 ORF clone pFN21AE0560

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Halotag ORF Clone pFN21AE0560

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Clone info.

Species

human

Clone Name

pFN21AE0560 Sequence (with vector) Restriction Map

Product ID

FHC05780

Flexi Clone PID

FXC05780

ORF Sequence

DNA sequence (1392 bp)
Protein sequence (464 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463813]

Gene

KIEE0560

Symbol

KRT28

Alias : K25IRS4, KRT25D

Description

keratin 28, type I

Original Clone ID

oc01260

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1392 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 464 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11376.1 9.1e-127 100.0 KRT28 CCDS11375.1 2e-95 77.1 KRT27 CCDS11373.1 1.1e-89 74.0 KRT25 CCDS11374.1 7e-84 68.4 KRT26 CCDS11377.1 4.3e-62 60.6 KRT10 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002957 168 181 PR01248 Keratin IPR002957 189 212 PR01248 Keratin IPR002957 243 263 PR01248 Keratin IPR002957 315 330 PR01248 Keratin IPR002957 341 367 PR01248 Keratin HMMPfam IPR016044 85 398 PF00038 Filament
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_853513 464 616677 100.0 100.0 NP_853515 459 616676 77.1 99.6 NP_853512 450 616646,616760 74.0 97.0 XP_011522716 448 616646,616760 74.2 95.9 NP_853517 468 616675 68.4 99.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0560 Download

>KIEE0560  1392 bp
ATGTCTCTCCAATTTTCTAATGGATCCAGGCATGTTTGCTTAAGGTCTGGAGCTGGATCT
GTCAGACCCCTCAATGGAGGTGCAGGCTTTGCAGGCAGCAGTGCATGTGGTGGCTCTGTT
GCTGGAAGTGAATTTTCCTGTGCCTTGGGAGGGGGCTTGGGCAGTGTTCCTGGTGGGAGC
CATGCTGGTGGTGCCCTTGGAAATGCTGCTTGTATTGGCTTTGCTGGAAGCGAAGGGGGA
CTCCTCTCTGGAAATGAGAAGGTGACCATGCAAAATCTTAATGACCGCTTGGCATCCTAC
CTGGATAATGTGCGAGCTCTGGAGGAGGCAAATGCTGAATTAGAGAGAAAAATCAAGGGT
TGGTATGAAAAATACGGACCTGGATCTTGCCGTGGACTTGATCATGACTATAGCAGATAT
CACCTAACAATTGAGGATCTTAAGAATAAGATTATCTCCTCCACTACTACTAATGCTAAT
GTCATTCTGCAGATTGATAATGCCAGACTGGCTGCTGATGATTTCAGGCTAAAGTATGAA
AATGAGCTCACCCTTCACCAAAACGTAGAGGCCGACATCAACGGATTACGGCGAGTCCTG
GACGAGCTGACGCTCTGCAGGACCGACCAGGAGCTGCAATATGAGTCTCTGAGTGAGGAG
ATGACATATCTCAAAAAGAACCACGAAGAGGAGATGAAGGCTCTGCAGTGCGCGGCTGGG
GGCAACGTGAACGTGGAGATGAACGCGGCCCCGGGGGTAGACCTCGCGGTTTTGTTGAAC
AACATGCGAGCGGAGTACGAAGCCCTTGCAGAGCAGAACCGCAAGGACGCGGAGGCCTGG
TTCAATGAGAAGAGCGCCTCGCTGCAGCAACAGATCTCCCACGACTCAGGCGCAGCCACT
TTCGCCCGGAGCCAGCTCACCGAGATGAGGCGCACCCTGCAGACCCTGGAGATCCAGCTG
CAGTCCCTGATGGCCACGAAACACTCCCTGGAGTGCTCCTTGACAGAGACCGAGAGCAAC
TACTGTACGCAGCTGGCGCAGATCCAGGCTCAGATCGGGGCCCTGGAGGAGCAGCTGCAC
CAGGTCAGAACCGAGACCGAGGGCCAGAAGCTGGAGTATGAGCATCTCCTCGATGTCAAG
GTCCACTTGGAAAAAGAAATTGAGACCTACTGCCGCCTGATAGATGGAGATGGAAATTCA
TGCTCCAAATCAAAGGGCTTTGGATCAGGAAGCCCTGGGAATTCATCTAAAGATTTATCC
AAAACCACACTGGTAAAGACAGTGGTTGAAGAGCTAGATCAACGTGGTAAAGTTCTTTCA
TCAAGGATTCACTCCATTGAAGAAAAGACATCTAAAATGACCAACGGCAAGACAGAACAA
AGGGTTCCTTTC

ORF Protein

Cloned ORF protein sequence for pFN21AE0560 Download

>KIEE0560  464 aa
MSLQFSNGSRHVCLRSGAGSVRPLNGGAGFAGSSACGGSVAGSEFSCALGGGLGSVPGGS
HAGGALGNAACIGFAGSEGGLLSGNEKVTMQNLNDRLASYLDNVRALEEANAELERKIKG
WYEKYGPGSCRGLDHDYSRYHLTIEDLKNKIISSTTTNANVILQIDNARLAADDFRLKYE
NELTLHQNVEADINGLRRVLDELTLCRTDQELQYESLSEEMTYLKKNHEEEMKALQCAAG
GNVNVEMNAAPGVDLAVLLNNMRAEYEALAEQNRKDAEAWFNEKSASLQQQISHDSGAAT
FARSQLTEMRRTLQTLEIQLQSLMATKHSLECSLTETESNYCTQLAQIQAQIGALEEQLH
QVRTETEGQKLEYEHLLDVKVHLEKEIETYCRLIDGDGNSCSKSKGFGSGSPGNSSKDLS
KTTLVKTVVEELDQRGKVLSSRIHSIEEKTSKMTNGKTEQRVPF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0560 Download

>pFN21AE0560  6097 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCTCTCCAATTTTCTAATGGATCCAGGCATGT
TTGCTTAAGGTCTGGAGCTGGATCTGTCAGACCCCTCAATGGAGGTGCAGGCTTTGCAGG
CAGCAGTGCATGTGGTGGCTCTGTTGCTGGAAGTGAATTTTCCTGTGCCTTGGGAGGGGG
CTTGGGCAGTGTTCCTGGTGGGAGCCATGCTGGTGGTGCCCTTGGAAATGCTGCTTGTAT
TGGCTTTGCTGGAAGCGAAGGGGGACTCCTCTCTGGAAATGAGAAGGTGACCATGCAAAA
TCTTAATGACCGCTTGGCATCCTACCTGGATAATGTGCGAGCTCTGGAGGAGGCAAATGC
TGAATTAGAGAGAAAAATCAAGGGTTGGTATGAAAAATACGGACCTGGATCTTGCCGTGG
ACTTGATCATGACTATAGCAGATATCACCTAACAATTGAGGATCTTAAGAATAAGATTAT
CTCCTCCACTACTACTAATGCTAATGTCATTCTGCAGATTGATAATGCCAGACTGGCTGC
TGATGATTTCAGGCTAAAGTATGAAAATGAGCTCACCCTTCACCAAAACGTAGAGGCCGA
CATCAACGGATTACGGCGAGTCCTGGACGAGCTGACGCTCTGCAGGACCGACCAGGAGCT
GCAATATGAGTCTCTGAGTGAGGAGATGACATATCTCAAAAAGAACCACGAAGAGGAGAT
GAAGGCTCTGCAGTGCGCGGCTGGGGGCAACGTGAACGTGGAGATGAACGCGGCCCCGGG
GGTAGACCTCGCGGTTTTGTTGAACAACATGCGAGCGGAGTACGAAGCCCTTGCAGAGCA
GAACCGCAAGGACGCGGAGGCCTGGTTCAATGAGAAGAGCGCCTCGCTGCAGCAACAGAT
CTCCCACGACTCAGGCGCAGCCACTTTCGCCCGGAGCCAGCTCACCGAGATGAGGCGCAC
CCTGCAGACCCTGGAGATCCAGCTGCAGTCCCTGATGGCCACGAAACACTCCCTGGAGTG
CTCCTTGACAGAGACCGAGAGCAACTACTGTACGCAGCTGGCGCAGATCCAGGCTCAGAT
CGGGGCCCTGGAGGAGCAGCTGCACCAGGTCAGAACCGAGACCGAGGGCCAGAAGCTGGA
GTATGAGCATCTCCTCGATGTCAAGGTCCACTTGGAAAAAGAAATTGAGACCTACTGCCG
CCTGATAGATGGAGATGGAAATTCATGCTCCAAATCAAAGGGCTTTGGATCAGGAAGCCC
TGGGAATTCATCTAAAGATTTATCCAAAACCACACTGGTAAAGACAGTGGTTGAAGAGCT
AGATCAACGTGGTAAAGTTCTTTCATCAAGGATTCACTCCATTGAAGAAAAGACATCTAA
AATGACCAACGGCAAGACAGAACAAAGGGTTCCTTTCGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT