(Circular) (Six-base) MAPSORT of: pFN21AE0560.seq Check: 8969 from: 1 to: 6097 pFN21AE0560 6097 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2763 3440 1937 Size: 826 677 4594 Fragments arranged by size: 4594 826 677 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4160 5544 5812 1632 Size: 2528 1384 268 1917 Fragments arranged by size: 2528 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2044 2444 820 Size: 197 1027 400 4473 Fragments arranged by size: 4473 1027 400 197 AflIII A'CryG_T Cuts at: 1203 4996 5973 1203 Size: 3793 977 1327 Fragments arranged by size: 3793 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2828 2860 2828 Size: 32 6065 AlwNI CAG_nnn'CTG Cuts at: 2638 2950 2971 3115 3840 5412 2638 Size: 312 21 144 725 1572 3323 Fragments arranged by size: 3323 1572 725 312 144 21 ApaI G_GGCC'C Cuts at: 3067 3067 Size: 6097 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2135 3199 3244 3406 3632 3784 5893 1493 Size: 642 1064 45 162 226 152 2109 1697 Fragments arranged by size: 2109 1697 1064 642 226 162 152 45 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2755 3422 1870 Size: 73 812 667 4545 Fragments arranged by size: 4545 812 667 73 BamHI G'GATC_C Cuts at: 2027 3427 3794 2027 Size: 1400 367 4330 Fragments arranged by size: 4330 1400 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2195 3418 4873 618 Size: 325 217 627 408 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 408 325 217 BanII G_rGCy'C Cuts at: 1391 2323 2553 3067 3416 1391 Size: 932 230 514 349 4072 Fragments arranged by size: 4072 932 514 349 230 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2792 2826 4288 4322 1903 Size: 34 855 34 1462 34 3678 Fragments arranged by size: 3678 1462 855 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 2404 1548 Size: 856 5241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2513 4708 136 Size: 107 122 71 631 605 841 2195 1525 Fragments arranged by size: 2195 1525 841 631 605 122 107 71 BglII A'GATC_T Cuts at: 2877 6092 2877 Size: 3215 2882 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 2200 3067 4158 5314 1790 Size: 410 867 1091 1156 2573 Fragments arranged by size: 2573 1156 1091 867 410 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6097 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3828 4786 442 Size: 416 53 83 399 24 2411 958 1753 Fragments arranged by size: 2411 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2073 2345 2972 3014 3089 3137 4757 1239 Size: 834 272 627 42 75 48 1620 2579 Fragments arranged by size: 2579 1620 834 627 272 75 48 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3005 3092 3161 4760 882 Size: 617 1506 87 69 1599 2219 Fragments arranged by size: 2219 1599 1506 617 87 69 BsaAI yAC'GTr Cuts at: 493 1204 3888 493 Size: 711 2684 2702 Fragments arranged by size: 2702 2684 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3904 4286 4921 275 Size: 53 83 186 936 2371 382 635 1451 Fragments arranged by size: 2371 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2828 2858 1178 Size: 30 165 30 1425 30 4417 Fragments arranged by size: 4417 1425 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2342 2452 2675 2708 3086 3122 1186 Size: 1051 105 110 223 33 378 36 4161 Fragments arranged by size: 4161 1051 378 223 110 105 36 33 BseYI C'CCAG_C Cuts at: 2720 5300 5796 2720 Size: 2580 496 3021 Fragments arranged by size: 3021 2580 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2106 3065 1163 Size: 573 370 959 4195 Fragments arranged by size: 4195 959 573 370 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3547 4308 5336 1540 Size: 275 186 1546 761 1028 2301 Fragments arranged by size: 2301 1546 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2323 2553 3002 4158 4243 5314 2323 Size: 230 449 1156 85 1071 3106 Fragments arranged by size: 3106 1156 1071 449 230 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2200 2323 2553 3002 3067 3416 4158 Size: 399 410 123 230 449 65 349 742 Cuts at: 4158 4243 5314 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 410 399 349 230 123 85 65 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 2407 3986 5716 2407 Size: 1579 1730 2788 Fragments arranged by size: 2788 1730 1579 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 844 Size: 2608 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2795 3984 1394 Size: 429 972 1189 3507 Fragments arranged by size: 3507 1189 972 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4741 4928 1700 Size: 110 2931 187 2869 Fragments arranged by size: 2931 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 2093 794 Size: 1299 4798 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 2266 1184 Size: 1082 5015 BstXI CCAn_nnnn'nTGG Cuts at: 2192 2192 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2027 2060 2387 2440 2877 2955 3045 3427 Size: 55 33 327 53 437 78 90 382 Cuts at: 3427 3794 4179 4196 5648 5734 5746 6092 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 437 385 382 367 346 327 90 86 78 55 53 33 17 12 BtgI C'CryG_G Cuts at: 513 1065 2395 513 Size: 552 1330 4215 Fragments arranged by size: 4215 1330 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 2110 2173 2717 3613 4407 4427 2110 Size: 63 544 896 794 20 3780 Fragments arranged by size: 3780 896 794 544 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2976 3544 4433 Size: 54 1425 50 275 18 1146 568 889 Cuts at: 4433 6062 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1146 889 568 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 2849 3288 4027 1353 Size: 1332 164 439 739 3423 Fragments arranged by size: 3423 1332 739 439 164 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2321 2551 2321 Size: 230 5867 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2345 2972 3014 3089 3137 4160 Size: 393 441 272 627 42 75 48 1023 Cuts at: 4160 4757 5544 5812 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1023 787 627 597 441 393 272 268 75 48 42 EcoO109I rG'GnC_Cy Cuts at: 3063 3064 3063 Size: 1 6096 EcoRI G'AATT_C Cuts at: 3244 3406 3784 3244 Size: 162 378 5557 Fragments arranged by size: 5557 378 162 EcoRV GAT'ATC Cuts at: 2423 2423 Size: 6097 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3166 3408 3166 Size: 242 5855 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 2862 5244 1678 Size: 1184 2382 2531 Fragments arranged by size: 2531 2382 1184 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2656 2688 2689 2721 3037 Size: 32 135 32 1084 32 1 32 316 Cuts at: 3037 3069 4738 4770 4812 4844 1373 Size: 32 1669 32 42 32 2626 Fragments arranged by size: 2626 1669 1084 316 135 42 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3687 669 Size: 988 281 1503 246 3079 Fragments arranged by size: 3079 1503 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 2433 3696 2433 Size: 1263 4834 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5211 5395 1305 Size: 3906 184 2007 Fragments arranged by size: 3906 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2978 10 Size: 54 1425 343 1146 3129 Fragments arranged by size: 3129 1425 1146 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2190 2927 4055 4414 4573 518 Size: 648 1024 737 1128 359 159 2042 Fragments arranged by size: 2042 1128 1024 737 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2962 3037 3079 3513 4190 5338 5583 1606 Size: 1356 75 42 434 677 1148 245 2120 Fragments arranged by size: 2120 1356 1148 677 434 245 75 42 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5545 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2039 2112 2791 3455 5000 1132 Size: 97 810 73 679 664 1545 2229 Fragments arranged by size: 2229 1545 810 679 664 97 73 PasI CC'CwG_GG Cuts at: 1348 3239 1348 Size: 1891 4206 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 3115 1108 Size: 2007 4090 PfoI T'CCnGG_A Cuts at: 2601 2601 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2828 2860 4166 4198 5679 5711 2828 Size: 32 1306 32 1481 32 3214 Fragments arranged by size: 3214 1481 1306 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 3063 3063 Size: 6097 PstI C_TGCA'G Cuts at: 830 2496 2624 2712 2871 2946 2967 3449 830 Size: 1666 128 88 159 75 21 482 3478 Fragments arranged by size: 3478 1666 482 159 128 88 75 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 2962 3037 3079 2962 Size: 75 42 5980 Fragments arranged by size: 5980 75 42 SacI G_AGCT'C Cuts at: 2323 2553 2323 Size: 230 5867 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SapI GCTCTTCn'nnn_ Cuts at: 2849 3288 2849 Size: 439 5658 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SexAI A'CCwGG_T Cuts at: 3084 3084 Size: 6097 SfcI C'TryA_G Cuts at: 826 1045 2413 2492 2620 2708 2867 2942 2963 Size: 219 1368 79 128 88 159 75 21 Cuts at: 2963 3445 4580 5261 5452 826 Size: 482 1135 681 191 1471 Fragments arranged by size: 1471 1368 1135 681 482 219 191 159 128 88 79 75 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 2757 3424 1872 Size: 885 667 4545 Fragments arranged by size: 4545 885 667 SmlI C'TyrA_G Cuts at: 820 1017 1943 2044 2444 4201 5102 5364 820 Size: 197 926 101 400 1757 901 262 1553 Fragments arranged by size: 1757 1553 926 901 400 262 197 101 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 2840 1517 Size: 1323 4774 StyI C'CwwG_G Cuts at: 513 1065 2148 2200 3536 513 Size: 552 1083 52 1336 3074 Fragments arranged by size: 3074 1336 1083 552 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2642 3029 3116 4124 4141 4294 4479 Size: 1255 1062 387 87 1008 17 153 185 Cuts at: 4479 4924 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1062 1008 445 387 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4343 6055 96 Size: 274 80 33 51 1443 2366 1712 138 Fragments arranged by size: 2366 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6057 6089 6057 Size: 32 6065 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2021 3271 2021 Size: 1250 4847 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 2755 3422 1870 Size: 885 667 4545 Fragments arranged by size: 4545 885 667 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI