KRT25 ORF clone pFN21AE0557

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0557

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Clone info.

Species

human

Clone Name

pFN21AE0557 Sequence (with vector) Restriction Map

Product ID

FHC05779

Flexi Clone PID

FXC05779

ORF Sequence

DNA sequence (1350 bp)
Protein sequence (450 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463812]

Gene

KIEE0557

Symbol

KRT25

Alias : ARWH3, KRT24IRS1, KRT25A

Description

keratin 25, type I

Original Clone ID

oc01257

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1350 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 450 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11373.1 6.2e-122 100.0 KRT25 CCDS11375.1 6.4e-98 81.3 KRT27 CCDS11376.1 1.9e-89 74.0 KRT28 CCDS11374.1 1.8e-83 71.7 KRT26 CCDS11377.1 3.5e-60 62.2 KRT10 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002957 161 174 PR01248 Keratin IPR002957 182 205 PR01248 Keratin IPR002957 236 256 PR01248 Keratin IPR002957 308 323 PR01248 Keratin IPR002957 334 360 PR01248 Keratin HMMPfam IPR016044 78 391 PF00038 Filament
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_853512 450 616646,616760 100.0 100.0 XP_011522716 448 616646,616760 99.6 98.9 NP_853515 459 616676 81.3 99.3 NP_853513 464 616677 74.0 99.3 NP_853517 468 616675 71.7 99.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0557 Download

>KIEE0557  1350 bp
ATGTCTCTTCGACTTTCCAGTGCATCCAGGAGGTCCTGTCCTCGTCCCACCACTGGATCA
CTCAGACTCTATGGTGGGGGAACCAGCTTTGGTACTGGAAATTCTTGTGGCATTTCAGGG
ATTGGAAGTGGCTTCTCTAGTGCCTTCGGAGGCAGCTCATCGGGAGGAAACACAGGGGGA
GGTAATCCCTGTGCTGGCTTCACTGTGAATGAGCGGGGGCTCCTTTCTGGCAATGAGAAG
GTGACCATGCAGAACCTCAATGACCGCCTGGCATCCTACCTGGACAGTGTGCATGCTCTG
GAGGAGGCCAACGCTGACCTGGAGCAGAAGATCAAGGGCTGGTATGAGAAATTTGGGCCT
GGCTCTTGCCGTGGTCTTGATCATGACTATAGCAGATATTTCCCAATAATTGATGACCTT
AAAAATCAGATCATCGCATCCACCACCAGCAATGCTAATGCTGTTCTGCAGATCGATAAT
GCCAGGCTTACAGCTGATGATTTCAGACTCAAGTATGAAAATGAGCTGGCTCTTCACCAG
AGTGTAGAGGCTGATGTCAATGGGTTACGAAGAGTTTTGGATGAAATAACCCTGTGCAGA
ACAGATCTGGAGATTCAGTATGAAACCCTGAGTGAGGAGATGACTTACCTCAAAAAGAAC
CATAAAGAGGAAATGCAAGTTCTGCAGTGCGCAGCTGGAGGCAACGTGAACGTGGAGATG
AACGCAGCCCCCGGGGTGGACCTCACAGTTCTGCTGAACAACATGCGAGCTGAGTACGAA
GCCCTTGCAGAGCAGAACCGCAGGGACGCGGAGGCCTGGTTCAACGAGAAGAGCGCCTCC
CTGCAGCAGCAGATCTCTGAGGATGTCGGAGCCACAACCTCAGCCCGGAATGAGCTGACT
GAAATGAAGCGCACTCTTCAAACCCTGGAAATTGAACTTCAGTCTCTCCTAGCCACGAAA
CACTCCCTGGAGTGCTCCTTGACAGAGACCGAGAGCAACTACTGTGCGCAGCTGGCGCAG
ATCCAGGCTCAGATCGGGGCCCTGGAGGAGCAGCTGCACCAGGTCAGAACCGAGACCGAG
GGCCAGAAGCTGGAGTATGAGCAGCTCCTGGACATCAAGCTCCACCTGGAAAAAGAAATT
GAGACCTACTGTCTCCTTATAGGAGGAGATGATGGAGCCTGTAAGTCTGGGGGTTACAAG
TCTAAAGATTATGGATCTGGAAATGTGGGAAGTCAAGTCAAAGACCCAGCCAAAGCCATA
GTGGTTAAGAAAGTTCTTGAGGAGGTAGACCAACGCAGCAAAATACTTACCACCAGGCTC
CACTCCCTGGAAGAGAAATCTCAAAGCAAT

ORF Protein

Cloned ORF protein sequence for pFN21AE0557 Download

>KIEE0557  450 aa
MSLRLSSASRRSCPRPTTGSLRLYGGGTSFGTGNSCGISGIGSGFSSAFGGSSSGGNTGG
GNPCAGFTVNERGLLSGNEKVTMQNLNDRLASYLDSVHALEEANADLEQKIKGWYEKFGP
GSCRGLDHDYSRYFPIIDDLKNQIIASTTSNANAVLQIDNARLTADDFRLKYENELALHQ
SVEADVNGLRRVLDEITLCRTDLEIQYETLSEEMTYLKKNHKEEMQVLQCAAGGNVNVEM
NAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFNEKSASLQQQISEDVGATTSARNELT
EMKRTLQTLEIELQSLLATKHSLECSLTETESNYCAQLAQIQAQIGALEEQLHQVRTETE
GQKLEYEQLLDIKLHLEKEIETYCLLIGGDDGACKSGGYKSKDYGSGNVGSQVKDPAKAI
VVKKVLEEVDQRSKILTTRLHSLEEKSQSN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0557 Download

>pFN21AE0557  6055 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCTCTTCGACTTTCCAGTGCATCCAGGAGGTC
CTGTCCTCGTCCCACCACTGGATCACTCAGACTCTATGGTGGGGGAACCAGCTTTGGTAC
TGGAAATTCTTGTGGCATTTCAGGGATTGGAAGTGGCTTCTCTAGTGCCTTCGGAGGCAG
CTCATCGGGAGGAAACACAGGGGGAGGTAATCCCTGTGCTGGCTTCACTGTGAATGAGCG
GGGGCTCCTTTCTGGCAATGAGAAGGTGACCATGCAGAACCTCAATGACCGCCTGGCATC
CTACCTGGACAGTGTGCATGCTCTGGAGGAGGCCAACGCTGACCTGGAGCAGAAGATCAA
GGGCTGGTATGAGAAATTTGGGCCTGGCTCTTGCCGTGGTCTTGATCATGACTATAGCAG
ATATTTCCCAATAATTGATGACCTTAAAAATCAGATCATCGCATCCACCACCAGCAATGC
TAATGCTGTTCTGCAGATCGATAATGCCAGGCTTACAGCTGATGATTTCAGACTCAAGTA
TGAAAATGAGCTGGCTCTTCACCAGAGTGTAGAGGCTGATGTCAATGGGTTACGAAGAGT
TTTGGATGAAATAACCCTGTGCAGAACAGATCTGGAGATTCAGTATGAAACCCTGAGTGA
GGAGATGACTTACCTCAAAAAGAACCATAAAGAGGAAATGCAAGTTCTGCAGTGCGCAGC
TGGAGGCAACGTGAACGTGGAGATGAACGCAGCCCCCGGGGTGGACCTCACAGTTCTGCT
GAACAACATGCGAGCTGAGTACGAAGCCCTTGCAGAGCAGAACCGCAGGGACGCGGAGGC
CTGGTTCAACGAGAAGAGCGCCTCCCTGCAGCAGCAGATCTCTGAGGATGTCGGAGCCAC
AACCTCAGCCCGGAATGAGCTGACTGAAATGAAGCGCACTCTTCAAACCCTGGAAATTGA
ACTTCAGTCTCTCCTAGCCACGAAACACTCCCTGGAGTGCTCCTTGACAGAGACCGAGAG
CAACTACTGTGCGCAGCTGGCGCAGATCCAGGCTCAGATCGGGGCCCTGGAGGAGCAGCT
GCACCAGGTCAGAACCGAGACCGAGGGCCAGAAGCTGGAGTATGAGCAGCTCCTGGACAT
CAAGCTCCACCTGGAAAAAGAAATTGAGACCTACTGTCTCCTTATAGGAGGAGATGATGG
AGCCTGTAAGTCTGGGGGTTACAAGTCTAAAGATTATGGATCTGGAAATGTGGGAAGTCA
AGTCAAAGACCCAGCCAAAGCCATAGTGGTTAAGAAAGTTCTTGAGGAGGTAGACCAACG
CAGCAAAATACTTACCACCAGGCTCCACTCCCTGGAAGAGAAATCTCAAAGCAATGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT