(Circular) (Six-base) MAPSORT of: pFN21AE0557.seq Check: 1859 from: 1 to: 6055 pFN21AE0557 6055 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3291 3398 1937 Size: 1354 107 4594 Fragments arranged by size: 4594 1354 107 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2927 4118 5502 5770 1632 Size: 1295 1191 1384 268 1917 Fragments arranged by size: 1917 1384 1295 1191 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 4954 5931 1203 Size: 3751 977 1327 Fragments arranged by size: 3751 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 3153 4784 3153 Size: 1631 4424 AleI CACnn'nnGTG Cuts at: 1166 2544 1166 Size: 1378 4677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2807 2839 2807 Size: 32 6023 AlwNI CAG_nnn'CTG Cuts at: 3094 3112 3798 5370 3094 Size: 18 686 1572 3779 Fragments arranged by size: 3779 1572 686 18 ApaI G_GGCC'C Cuts at: 3046 3046 Size: 6055 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 2104 2354 3364 3590 3742 5851 1493 Size: 611 250 1010 226 152 2109 1697 Fragments arranged by size: 2109 1697 1010 611 250 226 152 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2735 3380 1870 Size: 73 792 645 4545 Fragments arranged by size: 4545 792 645 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3376 4831 618 Size: 325 217 627 1589 1455 1842 Fragments arranged by size: 1842 1589 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2226 3046 3374 1391 Size: 835 820 328 4072 Fragments arranged by size: 4072 835 820 328 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5084 BbvCI CC'TCA_GC Cuts at: 2884 2884 Size: 6055 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2771 2805 4246 4280 1903 Size: 34 834 34 1441 34 3678 Fragments arranged by size: 3678 1441 834 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 2383 1548 Size: 835 5220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4666 136 Size: 107 122 71 631 605 2994 1525 Fragments arranged by size: 2994 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2608 2856 6050 2608 Size: 248 3194 2613 Fragments arranged by size: 3194 2613 248 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 3046 4116 5272 1790 Size: 1256 1070 1156 2573 Fragments arranged by size: 2573 1256 1156 1070 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3786 4744 442 Size: 416 53 83 399 24 2369 958 1753 Fragments arranged by size: 2369 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2345 2633 2721 2993 3068 3116 4715 Size: 1085 21 288 88 272 75 48 1599 Cuts at: 4715 1239 Size: 2579 Fragments arranged by size: 2579 1599 1085 288 272 88 75 48 21 Bpu10I CC'TnA_GC Cuts at: 2884 2884 Size: 6055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 3302 4180 5045 5343 2498 Size: 804 878 865 298 3210 Fragments arranged by size: 3210 878 865 804 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2984 3071 3140 4718 882 Size: 617 1485 87 69 1578 2219 Fragments arranged by size: 2219 1578 1485 617 87 69 BsaAI yAC'GTr Cuts at: 493 1204 3846 493 Size: 711 2642 2702 Fragments arranged by size: 2702 2642 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3862 4244 4879 275 Size: 53 83 186 936 2329 382 635 1451 Fragments arranged by size: 2329 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4488 4886 5160 5307 5718 4488 Size: 398 274 147 411 4825 Fragments arranged by size: 4825 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2026 2056 2606 2636 2694 Size: 30 165 30 623 30 550 30 58 Cuts at: 2694 2724 2807 2837 1178 Size: 30 83 30 4396 Fragments arranged by size: 4396 623 550 165 83 58 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2321 2654 3065 3183 3299 1186 Size: 1135 333 411 118 116 3942 Fragments arranged by size: 3942 1135 411 333 118 116 BseYI C'CCAG_C Cuts at: 3250 5258 5754 3250 Size: 2008 496 3551 Fragments arranged by size: 3551 2008 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2620 3044 1163 Size: 573 884 424 4174 Fragments arranged by size: 4174 884 573 424 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 4266 5294 1540 Size: 275 186 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2981 4116 4201 5272 2981 Size: 1135 85 1071 3764 Fragments arranged by size: 3764 1135 1071 85 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2981 3046 3374 4116 4201 5272 Size: 399 436 755 65 328 742 85 1071 Cuts at: 5272 1391 Size: 2174 Fragments arranged by size: 2174 1071 755 742 436 399 328 85 65 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 2386 3944 5674 2386 Size: 1558 1730 2767 Fragments arranged by size: 2767 1730 1558 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3410 844 Size: 2566 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2218 3942 1394 Size: 429 395 1724 3507 Fragments arranged by size: 3507 1724 429 395 BsrDI GCAATG_nn' Cuts at: 65 1248 2242 2461 3358 4550 4724 65 Size: 1183 994 219 897 1192 174 1396 Fragments arranged by size: 1396 1192 1183 994 897 219 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4699 4886 1700 Size: 110 2889 187 2869 Fragments arranged by size: 2889 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 4115 5127 4115 Size: 1012 5043 BstAPI GCAn_nnn'nTGC Cuts at: 794 2686 794 Size: 1892 4163 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 2245 1184 Size: 1061 4994 BstYI r'GATC_y Cuts at: 1972 2608 2856 3024 3218 3385 3752 4137 4154 Size: 636 248 168 194 167 367 385 17 Cuts at: 4154 5606 5692 5704 6050 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 636 385 367 346 248 194 168 167 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2374 513 Size: 552 1309 4194 Fragments arranged by size: 4194 1309 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2422 487 Size: 683 56 1196 4120 Fragments arranged by size: 4120 1196 683 56 BtsI GCAGTG_nn' Cuts at: 2696 3571 4365 4385 2696 Size: 875 794 20 4366 Fragments arranged by size: 4366 875 794 20 ClaI AT'CG_AT Cuts at: 1554 2478 1554 Size: 924 5131 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3502 4391 6020 Size: 54 1425 50 275 18 1672 889 1629 Cuts at: 6020 8 Size: 43 Fragments arranged by size: 1672 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2016 2541 2569 2828 2925 3330 3985 1353 Size: 663 525 28 259 97 405 655 3423 Fragments arranged by size: 3423 663 655 525 405 259 97 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4642 5016 5162 387 Size: 1064 138 3053 374 146 1280 Fragments arranged by size: 3053 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2345 2633 2721 2927 2993 3068 Size: 393 692 21 288 88 206 66 75 Cuts at: 3068 3116 4118 4715 5502 5770 1239 Size: 48 1002 597 787 268 1524 Fragments arranged by size: 1524 1002 787 692 597 393 288 268 206 88 75 66 48 21 EcoNI CCTnn'n_nnAGG Cuts at: 2636 2636 Size: 6055 EcoO109I rG'GnC_Cy Cuts at: 2037 3042 3043 2037 Size: 1005 1 5049 Fragments arranged by size: 5049 1005 1 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FspI TGC'GCA Cuts at: 2695 3012 4561 2695 Size: 317 1549 4189 Fragments arranged by size: 4189 1549 317 HaeII r_GCGC'y Cuts at: 1678 2841 5202 1678 Size: 1163 2361 2531 Fragments arranged by size: 2531 2361 1163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 3016 3048 3139 Size: 32 135 32 454 32 958 32 91 Cuts at: 3139 3171 3203 3235 4696 4728 4770 4802 1373 Size: 32 32 32 1461 32 42 32 2626 Fragments arranged by size: 2626 1461 958 454 135 91 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2851 5169 5353 1305 Size: 1546 2318 184 2007 Fragments arranged by size: 2318 2007 1546 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4233 Fragments arranged by size: 4233 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2544 4013 4372 4531 518 Size: 648 1378 1469 359 159 2042 Fragments arranged by size: 2042 1469 1378 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2498 2699 3016 3058 3471 4148 5296 5541 Size: 892 201 317 42 413 677 1148 245 Cuts at: 5541 1606 Size: 2120 Fragments arranged by size: 2120 1148 892 677 413 317 245 201 42 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5503 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 2300 2770 3413 4958 1132 Size: 97 1071 470 643 1545 2229 Fragments arranged by size: 2229 1545 1071 643 470 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 3094 3331 1108 Size: 1986 237 3832 Fragments arranged by size: 3832 1986 237 PfoI T'CCnGG_A Cuts at: 2030 3111 2030 Size: 1081 4974 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2807 2839 4124 4156 5637 5669 2807 Size: 32 1285 32 1481 32 3193 Fragments arranged by size: 3193 1481 1285 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2037 2037 Size: 6055 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 3042 3042 Size: 6055 PstI C_TGCA'G Cuts at: 830 2475 2691 2850 3407 830 Size: 1645 216 159 557 3478 Fragments arranged by size: 3478 1645 557 216 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2498 2699 3016 3058 2498 Size: 201 317 42 5495 Fragments arranged by size: 5495 317 201 42 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SapI GCTCTTCn'nnn_ Cuts at: 2541 2828 2541 Size: 287 5768 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SexAI A'CCwGG_T Cuts at: 3063 3063 Size: 6055 SfcI C'TryA_G Cuts at: 826 1045 2392 2471 2687 2846 3403 4538 5219 Size: 219 1347 79 216 159 557 1135 681 Cuts at: 5219 5410 826 Size: 191 1471 Fragments arranged by size: 1471 1347 1135 681 557 219 216 191 159 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 2737 3382 1872 Size: 865 645 4545 Fragments arranged by size: 4545 865 645 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 3281 4159 5060 5322 820 Size: 197 926 570 768 878 901 262 1553 Fragments arranged by size: 1553 926 901 878 768 570 262 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 2300 3413 1132 Size: 1168 1113 3774 Fragments arranged by size: 3774 1168 1113 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 2819 1517 Size: 1302 4753 StyI C'CwwG_G Cuts at: 513 1065 3494 513 Size: 552 2429 3074 Fragments arranged by size: 3074 2429 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3008 3095 4082 4099 4252 4437 4882 Size: 1255 1428 87 987 17 153 185 445 Cuts at: 4882 325 Size: 1498 Fragments arranged by size: 1498 1428 1255 987 445 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4301 6013 96 Size: 274 80 33 51 1443 2324 1712 138 Fragments arranged by size: 2324 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2116 2148 2694 2726 6015 6047 2116 Size: 32 546 32 3289 32 2124 Fragments arranged by size: 3289 2124 546 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6055 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 3262 2320 Size: 942 5113 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 2735 3380 1870 Size: 865 645 4545 Fragments arranged by size: 4545 865 645 XmnI GAAnn'nnTTC Cuts at: 4184 4184 Size: 6055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI