CRYBA4 ORF clone pFN21AE0495

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0495

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Clone info.

Species

human

Clone Name

pFN21AE0495 Sequence (with vector) Restriction Map

Product ID

FHC04642

Flexi Clone PID

FXC04642

ORF Sequence

DNA sequence (621 bp)
Protein sequence (207 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528112]

Gene

KIEE0495

Symbol

CRYBA4

Alias : CTRCT23, CYRBA4, MCOPCT4

Description

crystallin, beta A4

Original Clone ID

oc01195

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 621 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 207 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13841.1 2.3e-96 100.0 CRYBA4 CCDS11249.1 2.6e-66 68.3 CRYBA1 CCDS2429.1 4.4e-48 54.5 CRYBA2 CCDS13840.1 9e-35 42.1 CRYBB1 CCDS13831.1 5.9e-31 41.6 CRYBB2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001064 60 79 PR01367 Beta/gamma crystallin IPR001064 80 94 PR01367 Beta/gamma crystallin IPR001064 118 137 PR01367 Beta/gamma crystallin IPR001064 181 190 PR01367 Beta/gamma crystallin HMMPfam IPR001064 24 107 PF00030 Beta/gamma crystallin IPR001064 118 205 PF00030 Beta/gamma crystallin HMMSmart IPR001064 24 108 SM00247 Beta/gamma crystallin IPR001064 117 205 SM00247 Beta/gamma crystallin ProfileScan IPR001064 23 62 PS50915 Beta/gamma crystallin IPR001064 63 109 PS50915 Beta/gamma crystallin IPR001064 116 157 PS50915 Beta/gamma crystallin IPR001064 158 206 PS50915 Beta/gamma crystallin
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016884087 207 123631,610425 100.0 100.0 XP_006724203 201 123631,610425 100.0 96.6 NP_001877 196 123631,610425 100.0 94.7 NP_005199 215 123610,600881 68.3 91.3 XP_016879687 316 123610,600881 68.3 91.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0495 Download

>KIEE0495  621 bp
ATGTTCCCTGGGCCTATCTCGGAAGGGGCCACAATGACCCTGCAATGCACAAAGTCAGCG
GGACCCTGGAAGATGGTGGTGTGGGATGAGGACGGCTTCCAGGGCCGGCGGCACGAGTTC
ACGGCCGAGTGCCCCAGCGTGCTGGAGCTTGGCTTCGAGACTGTGCGATCTTTGAAAGTG
CTGAGTGGAGCGTGGGTGGGCTTCGAGCATGCTGGCTTCCAAGGGCAGCAGTACATTCTG
GAACGAGGCGAATATCCAAGCTGGGATGCCTGGGGCGGCAACACGGCCTACCCCGCCGAG
AGGCTCACCTCCTTCCGGCCTGCGGCCTGTGCTAACCACCGTGACTCGAGGCTGACAATC
TTCGAGCAAGAGAACTTCCTGGGCAAGAAAGGAGAGCTGAGCGATGACTATCCTTCCCTC
CAGGCCATGGGATGGGAAGGCAATGAAGTAGGGTCCTTCCACGTCCACTCTGGGGCCTGG
GTTTGCTCCCAGTTTCCGGGCTACCGAGGATTTCAGTATGTGCTGGAATGCGATCACCAT
TCCGGTGACTACAAACATTTCCGGGAGTGGGGCTCTCATGCCCCGACCTTCCAGGTGCAG
AGCATCCGCAGGATCCAGCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE0495 Download

>KIEE0495  207 aa
MFPGPISEGATMTLQCTKSAGPWKMVVWDEDGFQGRRHEFTAECPSVLELGFETVRSLKV
LSGAWVGFEHAGFQGQQYILERGEYPSWDAWGGNTAYPAERLTSFRPAACANHRDSRLTI
FEQENFLGKKGELSDDYPSLQAMGWEGNEVGSFHVHSGAWVCSQFPGYRGFQYVLECDHH
SGDYKHFREWGSHAPTFQVQSIRRIQQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0495 Download

>pFN21AE0495  5326 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTCCCTGGGCCTATCTCGGAAGGGGCCACAAT
GACCCTGCAATGCACAAAGTCAGCGGGACCCTGGAAGATGGTGGTGTGGGATGAGGACGG
CTTCCAGGGCCGGCGGCACGAGTTCACGGCCGAGTGCCCCAGCGTGCTGGAGCTTGGCTT
CGAGACTGTGCGATCTTTGAAAGTGCTGAGTGGAGCGTGGGTGGGCTTCGAGCATGCTGG
CTTCCAAGGGCAGCAGTACATTCTGGAACGAGGCGAATATCCAAGCTGGGATGCCTGGGG
CGGCAACACGGCCTACCCCGCCGAGAGGCTCACCTCCTTCCGGCCTGCGGCCTGTGCTAA
CCACCGTGACTCGAGGCTGACAATCTTCGAGCAAGAGAACTTCCTGGGCAAGAAAGGAGA
GCTGAGCGATGACTATCCTTCCCTCCAGGCCATGGGATGGGAAGGCAATGAAGTAGGGTC
CTTCCACGTCCACTCTGGGGCCTGGGTTTGCTCCCAGTTTCCGGGCTACCGAGGATTTCA
GTATGTGCTGGAATGCGATCACCATTCCGGTGACTACAAACATTTCCGGGAGTGGGGCTC
TCATGCCCCGACCTTCCAGGTGCAGAGCATCCGCAGGATCCAGCAGGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT