Restriction map for
pFN21AE0495
(Circular) (Six-base) MAPSORT of: pFN21AE0495.seq Check: 2213 from: 1 to: 5326
pFN21AE0495 5326 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3136 278
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 1937
Size: 732 4594
Acc65I G'GTAC_C
Cuts at: 2647 2647
Size: 5326
AclI AA'CG_TT
Cuts at: 1563 3453 3826 1563
Size: 1890 373 3063
Fragments arranged by size:
3063 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3389 4773 5041 1632
Size: 1757 1384 268 1917
Fragments arranged by size:
1917 1757 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5129
AflIII A'CryG_T
Cuts at: 1203 4225 5202 1203
Size: 3022 977 1327
Fragments arranged by size:
3022 1327 977
AgeI A'CCGG_T
Cuts at: 4157 4157
Size: 5326
AhdI GACnn_n'nnGTC
Cuts at: 4055 4055
Size: 5326
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5326
AlwNI CAG_nnn'CTG
Cuts at: 3069 4641 3069
Size: 1572 3754
ApaLI G'TGCA_C
Cuts at: 3383 4539 3383
Size: 1156 4170
ApoI r'AATT_y
Cuts at: 1493 2635 2861 3013 5122 1493
Size: 1142 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3880 160
Size: 872 2848 1606
Fragments arranged by size:
2848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
AvaI C'yCGr_G
Cuts at: 1870 1943 2350 2651 1870
Size: 73 407 301 4545
Fragments arranged by size:
4545 407 301 73
BamHI G'GATC_C
Cuts at: 2616 2656 3023 2616
Size: 40 367 4919
Fragments arranged by size:
4919 367 40
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2647 4102 618
Size: 325 217 627 860 1455 1842
Fragments arranged by size:
1842 1455 860 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2579 2645 1391
Size: 1188 66 4072
Fragments arranged by size:
4072 1188 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3517 3551 1903
Size: 34 1580 34 3678
Fragments arranged by size:
3678 1580 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3218 4428 3218
Size: 1210 4116
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3937 136
Size: 107 122 71 631 605 2265 1525
Fragments arranged by size:
2265 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5321 5321
Size: 5326
BlpI GC'TnA_GC
Cuts at: 2402 2743 2402
Size: 341 4985
Bme1580I G_kGCm'C
Cuts at: 1790 2138 3387 4543 1790
Size: 348 1249 1156 2573
Fragments arranged by size:
2573 1249 1156 348
BmgBI CAC'GTC
Cuts at: 1318 2467 1318
Size: 1149 4177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2488 3057 4015
Size: 416 53 83 399 24 1071 569 958
Cuts at: 4015 442
Size: 1753
Fragments arranged by size:
1753 1071 958 569 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 5326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2408 3986 1239
Size: 929 240 1578 2579
Fragments arranged by size:
2579 1578 929 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3451 4316 4614 3451
Size: 865 298 4163
Fragments arranged by size:
4163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3989 882
Size: 617 2490 2219
Fragments arranged by size:
2490 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3117 493
Size: 711 1913 2702
Fragments arranged by size:
2702 1913 711
BsaBI GATnn'nnATC
Cuts at: 2412 5320 2412
Size: 2908 2418
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3133 3515 4150 275
Size: 53 83 186 936 1600 382 635 1451
Fragments arranged by size:
1600 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2546 3759 4157 4431 4578 4989 2546
Size: 1213 398 274 147 411 2883
Fragments arranged by size:
2883 1213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 1178
Size: 30 165 30 781 30 4290
Fragments arranged by size:
4290 781 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5326
BseYI C'CCAG_C
Cuts at: 2138 2265 4529 5025 2138
Size: 127 2264 496 2439
Fragments arranged by size:
2439 2264 496 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2621 1163
Size: 573 885 3868
Fragments arranged by size:
3868 885 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 2776 3537 4565 1540
Size: 275 186 129 646 761 1028 2301
Fragments arranged by size:
2301 1028 761 646 275 186 129
BsiHKAI G_wGCw'C
Cuts at: 3387 3472 4543 3387
Size: 85 1071 4170
Fragments arranged by size:
4170 1071 85
BsmI GAATG_Cn'
Cuts at: 2537 2837 2930 2537
Size: 300 93 4933
Fragments arranged by size:
4933 300 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2579 2645 3387 3472 4543 1391
Size: 399 348 441 66 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 441 399 348 85 66
BspEI T'CCGG_A
Cuts at: 4989 4989
Size: 5326
BspHI T'CATG_A
Cuts at: 3215 4945 3215
Size: 1730 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2614 2681 844
Size: 1770 67 3489
Fragments arranged by size:
3489 1770 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3213 1394
Size: 429 1390 3507
Fragments arranged by size:
3507 1390 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2054 2452 3821 3995 65
Size: 1183 806 398 1369 174 1396
Fragments arranged by size:
1396 1369 1183 806 398 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 3970 4157 1700
Size: 110 299 1861 187 2869
Fragments arranged by size:
2869 1861 299 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5326
BssSI C'ACGA_G
Cuts at: 2117 3386 4398 2117
Size: 1269 1012 3045
Fragments arranged by size:
3045 1269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5326
BstBI TT'CG_AA
Cuts at: 4130 4130
Size: 5326
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5326
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2146
Size: 5326
BstYI r'GATC_y
Cuts at: 1972 2616 2656 3023 3408 3425 4877 4963 4975
Size: 644 40 367 385 17 1452 86 12
Cuts at: 4975 5321 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 644 385 367 346 86 40
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2430 513
Size: 552 1365 3409
Fragments arranged by size:
3409 1365 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2421 487
Size: 683 56 1195 3392
Fragments arranged by size:
3392 1195 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3636 3656 2842
Size: 794 20 4512
Fragments arranged by size:
4512 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5291
DraI TTT'AAA
Cuts at: 2630 2630
Size: 5326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4333 809
Size: 507 3017 1802
Fragments arranged by size:
3017 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2773 3662
Size: 54 1425 50 275 18 297 646 889
Cuts at: 3662 5291 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 646 297 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2127 2773 1537
Size: 275 315 646 4090
Fragments arranged by size:
4090 646 315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3256 1353
Size: 1903 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3913 4287 4433 387
Size: 1064 138 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2408 3389 3986 4773 5041 1239
Size: 393 536 240 981 597 787 268 1524
Fragments arranged by size:
1524 981 787 597 536 393 268 240
EcoO109I rG'GnC_Cy
Cuts at: 2066 2457 2478 2066
Size: 391 21 4914
Fragments arranged by size:
4914 391 21
EcoRI G'AATT_C
Cuts at: 2635 3013 2635
Size: 378 4948
FspI TGC'GCA
Cuts at: 3832 3832
Size: 5326
HaeII r_GCGC'y
Cuts at: 1678 4473 1678
Size: 2795 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3967 3999 4041 4073 1373
Size: 32 135 32 2395 32 42 32 2626
Fragments arranged by size:
2626 2395 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 2916 669
Size: 988 281 732 246 3079
Fragments arranged by size:
3079 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5326
HpaI GTT'AAC
Cuts at: 2916 2916
Size: 5326
KpnI G_GTAC'C
Cuts at: 2651 2651
Size: 5326
MfeI C'AATT_G
Cuts at: 2925 2925
Size: 5326
MluI A'CGCG_T
Cuts at: 5202 5202
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4440 4624 1305
Size: 3135 184 2007
Fragments arranged by size:
3135 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3504
Fragments arranged by size:
3504 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 3284 3643 3802 518
Size: 648 1381 737 359 159 2042
Fragments arranged by size:
2042 1381 737 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2742 3419 4567 4812 1606
Size: 457 679 677 1148 245 2120
Fragments arranged by size:
2120 1148 679 677 457 245
NaeI GCC'GGC
Cuts at: 2111 2111
Size: 5326
NcoI C'CATG_G
Cuts at: 513 1065 2430 513
Size: 552 1365 3409
Fragments arranged by size:
3409 1365 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5326
NgoMIV G'CCGG_C
Cuts at: 2109 2109
Size: 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5326
NotI GC'GGCC_GC
Cuts at: 2773 2773
Size: 5326
NspI r_CATG'y
Cuts at: 1132 1229 2216 2684 4229 1132
Size: 97 987 468 1545 2229
Fragments arranged by size:
2229 1545 987 468 97
PasI CC'CwG_GG
Cuts at: 1348 2012 1348
Size: 664 4662
PciI A'CATG_T
Cuts at: 4225 4225
Size: 5326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2431 2436 1108
Size: 1323 5 3998
Fragments arranged by size:
3998 1323 5
PfoI T'CCnGG_A
Cuts at: 2565 2565
Size: 5326
PmeI GTTT'AAAC
Cuts at: 2630 2630
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 4908 4940 3395
Size: 32 1481 32 3781
Fragments arranged by size:
3781 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 2457 2066
Size: 391 4935
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5326
PsiI TTA'TAA
Cuts at: 2896 2896
Size: 5326
PspXI vC'TCGA_Gb
Cuts at: 2350 2350
Size: 5326
PstI C_TGCA'G
Cuts at: 830 2678 830
Size: 1848 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5326
SalI G'TCGA_C
Cuts at: 1936 2668 1936
Size: 732 4594
SanDI GG'GwC_CC
Cuts at: 2066 2066
Size: 5326
SbfI CC_TGCA'GG
Cuts at: 2678 2678
Size: 5326
ScaI AGT'ACT
Cuts at: 3574 5286 3574
Size: 1712 3614
SfcI C'TryA_G
Cuts at: 826 1045 2674 3809 4490 4681 826
Size: 219 1629 1135 681 191 1471
Fragments arranged by size:
1629 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5326
SmaI CCC'GGG
Cuts at: 1872 2653 1872
Size: 781 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2350 3430 4331 4593 820
Size: 197 926 407 1080 901 262 1553
Fragments arranged by size:
1553 1080 926 901 407 262 197
SnaBI TAC'GTA
Cuts at: 493 3117 493
Size: 2624 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5326
SphI G_CATG'C
Cuts at: 1132 2216 2684 1132
Size: 1084 468 3774
Fragments arranged by size:
3774 1084 468
SspI AAT'ATT
Cuts at: 5 52 3250 5
Size: 47 3198 2081
Fragments arranged by size:
3198 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5326
StyI C'CwwG_G
Cuts at: 513 1065 2224 2430 2765 513
Size: 552 1159 206 335 3074
Fragments arranged by size:
3074 1159 552 335 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 3353 3370 3523 3708 4153 325
Size: 1255 608 1165 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1165 608 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 3572 5284
Size: 274 80 33 51 1443 258 1337 1712
Cuts at: 5284 96
Size: 138
Fragments arranged by size:
1712 1443 1337 274 258 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 5286 5318 2184
Size: 32 3070 32 2192
Fragments arranged by size:
3070 2192 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5326
XbaI T'CTAG_A
Cuts at: 2662 2662
Size: 5326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2471 2471
Size: 5326
XhoI C'TCGA_G
Cuts at: 1943 2350 1943
Size: 407 4919
XmaI C'CCGG_G
Cuts at: 1870 2651 1870
Size: 781 4545
XmnI GAAnn'nnTTC
Cuts at: 3455 3455
Size: 5326
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3134 276
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI