MRI1 ORF clone pFN21AE0443

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Halotag ORF Clone pFN21AE0443

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Clone info.

Species

human

Clone Name

pFN21AE0443 Sequence (with vector) Restriction Map

Product ID

FHC05979

Flexi Clone PID

FXC05979

ORF Sequence

DNA sequence (966 bp)
Protein sequence (322 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528180]

Gene

KIEE0443

Symbol

MRI1

Alias : M1Pi, MRDI, MTNA, Ypr118w

Description

methylthioribose-1-phosphate isomerase 1, transcript variant 2

Original Clone ID

oc01143

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 966 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 322 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12297.1 1.2e-135 100.0 MRI1 CCDS32923.1 1.7e-68 81.6 MRI1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000649 44 135 PF01008 Initiation factor 2B-related IPR000649 152 299 PF01008 Initiation factor 2B-related HMMTigr IPR011559 153 299 TIGR00524 Initiation factor 2B alpha/beta/delta
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 46 RGAPAIALVGCLSLAVELQ 64 PRIMARY 19
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_115661 322 615105 100.0 100.0 NP_001316501 340 615105 94.7 100.0 NP_001026897 369 615105 81.6 95.3 XP_011526658 322 615105 79.5 83.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0443 Download

>KIEE0443  966 bp
ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
GTCCGGGAGAGACGTGAAACGGAGCTATGCGAGCATTGGGAAGAGCATACCAGGCAGAGG
GAACTGCCACTGCGAGGGCCCCTGGGCGGGACTGTGCTCGAGACCCGGCCCTACAACCAG
GGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAGATCCCCGCCACCCTTATCACC
GACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTGTCAGCTGTGGTCGTGGGAGCT
GACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTGGGCACCTACCAGCTGGCCATT
GTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCCCCCAGCTCTTCATGTGACCTC
CGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGACCGGGCCAGGAGCTGACCGAT
GTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTTTGGAATCCTGCCTTCGATGTC
ACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAACTGGGGGTCTTTGCCCCTGAG
GAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGGGATGGAACCCTAGATGGACCC
CAGATG

ORF Protein

Cloned ORF protein sequence for pFN21AE0443 Download

>KIEE0443  322 aa
MTLEAIRYSRGSLQILDQLLLPKQSRYEAVGSVHQAWEAIRAMKVRGAPAIALVGCLSLA
VELQAGAGGPGLAALVAFVRDKLSFLVTARPTAVNMARAARDLADVAAREAEREGATEEA
VRERRETELCEHWEEHTRQRELPLRGPLGGTVLETRPYNQGARLTAFELVYEQIPATLIT
DSMVAAAMAHRGVSAVVVGADRVVANGDTANKVGTYQLAIVAKHHGIPFYVAAPSSSCDL
RLETGKEIIIEERPGQELTDVNGVRIAAPGIGVWNPAFDVTPHDLITGGIITELGVFAPE
ELRTALTTTISSRDGTLDGPQM

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0443 Download

>pFN21AE0443  5671 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTC
CCTGCAGATCCTAGACCAGCTGCTGCTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTC
GGTGCACCAGGCCTGGGAGGCCATCCGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGC
CCTGGTGGGCTGTCTCAGCCTCGCCGTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACT
CGCCGCGCTCGTGGCCTTCGTGCGCGACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCAC
CGCTGTCAACATGGCCCGCGCCGCCCGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGA
ACGGGAGGGCGCTACGGAAGAGGCGGTCCGGGAGAGACGTGAAACGGAGCTATGCGAGCA
TTGGGAAGAGCATACCAGGCAGAGGGAACTGCCACTGCGAGGGCCCCTGGGCGGGACTGT
GCTCGAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGA
GCAGATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGG
CGTGTCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAA
GGTGGGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGC
TGCCCCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGA
GCGACCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGG
AGTTTGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCAC
AGAACTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTC
CAGGGATGGAACCCTAGATGGACCCCAGATGGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT