(Circular) (Six-base) MAPSORT of: pFN21AE0443.seq Check: 2094 from: 1 to: 5671 pFN21AE0443 5671 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3734 5118 5386 1632 Size: 2102 1384 268 1917 Fragments arranged by size: 2102 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 4570 5547 1203 Size: 3367 977 1327 Fragments arranged by size: 3367 1327 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5671 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5671 AlwNI CAG_nnn'CTG Cuts at: 2062 2776 2810 3414 4986 2062 Size: 714 34 604 1572 2747 Fragments arranged by size: 2747 1572 714 604 34 ApaI G_GGCC'C Cuts at: 2445 2445 Size: 5671 ApaLI G'TGCA_C Cuts at: 2102 3728 4884 2102 Size: 1626 1156 2889 Fragments arranged by size: 2889 1626 1156 ApoI r'AATT_y Cuts at: 1493 2980 3206 3358 5467 1493 Size: 1487 226 152 2109 1697 Fragments arranged by size: 2109 1697 1487 226 152 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2328 2462 2996 1870 Size: 73 385 134 534 4545 Fragments arranged by size: 4545 534 385 134 73 BamHI G'GATC_C Cuts at: 3001 3368 3001 Size: 367 5304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2201 2645 2992 4447 Size: 325 217 627 357 57 444 347 1455 Cuts at: 4447 618 Size: 1842 Fragments arranged by size: 1842 1455 627 444 357 347 325 217 57 BanII G_rGCy'C Cuts at: 1391 2039 2099 2445 2491 2910 2990 1391 Size: 648 60 346 46 419 80 4072 Fragments arranged by size: 4072 648 419 346 80 60 46 BbeI G_GCGC'C Cuts at: 2148 2205 2148 Size: 57 5614 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3862 3896 1903 Size: 34 1925 34 3678 Fragments arranged by size: 3678 1925 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2117 2333 4282 Size: 107 122 71 631 605 445 216 1949 Cuts at: 4282 136 Size: 1525 Fragments arranged by size: 1949 1525 631 605 445 216 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 2252 3088 2252 Size: 836 4835 Bme1580I G_kGCm'C Cuts at: 1790 2106 2445 2648 3732 4888 1790 Size: 316 339 203 1084 1156 2573 Fragments arranged by size: 2573 1156 1084 339 316 203 BmgBI CAC'GTC Cuts at: 1318 2378 1318 Size: 1060 4611 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2894 3402 4360 Size: 416 53 83 399 24 1477 508 958 Cuts at: 4360 442 Size: 1753 Fragments arranged by size: 1753 1477 958 508 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2750 4331 1239 Size: 794 717 1581 2579 Fragments arranged by size: 2579 1581 794 717 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3796 4661 4959 3796 Size: 865 298 4508 Fragments arranged by size: 4508 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2459 2726 4334 882 Size: 617 960 267 1608 2219 Fragments arranged by size: 2219 1608 960 617 267 BsaAI yAC'GTr Cuts at: 493 1204 2695 3462 493 Size: 711 1491 767 2702 Fragments arranged by size: 2702 1491 767 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 2202 3478 3860 Size: 53 83 186 936 612 57 1276 382 Cuts at: 3860 4495 275 Size: 635 1451 Fragments arranged by size: 1451 1276 936 635 612 382 186 83 57 53 BsaWI w'CCGG_w Cuts at: 2796 2910 4104 4502 4776 4923 5334 2796 Size: 114 1194 398 274 147 411 3133 Fragments arranged by size: 3133 1194 411 398 274 147 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5446 Fragments arranged by size: 5446 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2918 1186 Size: 1732 3939 BseYI C'CCAG_C Cuts at: 2705 4874 5370 2705 Size: 2169 496 3006 Fragments arranged by size: 3006 2169 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5098 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2765 3121 3882 4910 1540 Size: 275 186 764 356 761 1028 2301 Fragments arranged by size: 2301 1028 764 761 356 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2463 2910 3732 3817 4888 2106 Size: 357 447 822 85 1071 2889 Fragments arranged by size: 2889 1071 822 447 357 85 BsmI GAATG_Cn' Cuts at: 2682 3182 3275 2682 Size: 500 93 5078 Fragments arranged by size: 5078 500 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2368 2368 Size: 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2099 2106 2445 2463 2491 2648 Size: 399 249 60 7 339 18 28 157 Cuts at: 2648 2910 2990 3732 3817 4888 1391 Size: 262 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 339 262 249 157 85 80 60 28 18 7 BspEI T'CCGG_A Cuts at: 2796 2910 5334 2796 Size: 114 2424 3133 Fragments arranged by size: 3133 2424 114 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3026 844 Size: 2182 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3558 1394 Size: 429 1735 3507 Fragments arranged by size: 3507 1735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4166 4340 65 Size: 1183 2918 174 1396 Fragments arranged by size: 2918 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2735 4315 4502 1700 Size: 110 925 1580 187 2869 Fragments arranged by size: 2869 1580 925 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 2228 3731 4743 2228 Size: 1503 1012 3156 Fragments arranged by size: 3156 1503 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5671 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2161 2641 2011 Size: 150 480 5041 Fragments arranged by size: 5041 480 150 BstYI r'GATC_y Cuts at: 1972 2046 2523 3001 3368 3753 3770 5222 5308 Size: 74 477 478 367 385 17 1452 86 Cuts at: 5308 5320 5666 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 478 477 385 367 346 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 2901 2901 Size: 5671 BtgI C'CryG_G Cuts at: 513 1065 2184 2565 2677 513 Size: 552 1119 381 112 3507 Fragments arranged by size: 3507 1119 552 381 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 2432 3187 3981 4001 2432 Size: 755 794 20 4102 Fragments arranged by size: 4102 794 755 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2658 3118 Size: 54 1425 50 275 18 321 507 460 Cuts at: 3118 4007 5636 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 507 460 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 2400 2716 2751 2941 3601 1353 Size: 999 48 316 35 190 660 3423 Fragments arranged by size: 3423 999 660 316 190 48 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4258 4632 4778 387 Size: 1064 138 2669 374 146 1280 Fragments arranged by size: 2669 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2908 2908 Size: 5671 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2750 3734 4331 5118 5386 1239 Size: 393 401 717 984 597 787 268 1524 Fragments arranged by size: 1524 984 787 717 597 401 393 268 EcoO109I rG'GnC_Cy Cuts at: 2441 2442 2441 Size: 1 5670 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2272 2272 Size: 5671 FspI TGC'GCA Cuts at: 4177 4177 Size: 5671 HaeII r_GCGC'y Cuts at: 1678 2148 2205 2352 4818 1678 Size: 470 57 147 2466 2531 Fragments arranged by size: 2531 2466 470 147 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2202 2234 4312 4344 4386 Size: 32 135 32 630 32 2078 32 42 Cuts at: 4386 4418 1373 Size: 32 2626 Fragments arranged by size: 2626 2078 630 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 3015 3261 669 Size: 988 281 349 728 246 3079 Fragments arranged by size: 3079 988 728 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KasI G'GCGC_C Cuts at: 2144 2201 2144 Size: 57 5614 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4785 4969 1305 Size: 3480 184 2007 Fragments arranged by size: 3480 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2660 10 Size: 54 1425 343 828 3021 Fragments arranged by size: 3021 1425 828 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 3629 3988 4147 518 Size: 648 969 1494 359 159 2042 Fragments arranged by size: 2042 1494 969 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2282 2588 2656 3087 3764 4912 5157 Size: 453 223 306 68 431 677 1148 245 Cuts at: 5157 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 453 431 306 245 223 68 NarI GG'CG_CC Cuts at: 2145 2202 2145 Size: 57 5614 NcoI C'CATG_G Cuts at: 513 1065 2565 2677 513 Size: 552 1500 112 3507 Fragments arranged by size: 3507 1500 552 112 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 3029 4574 1132 Size: 97 1800 1545 2229 Fragments arranged by size: 2229 1800 1545 97 PasI CC'CwG_GG Cuts at: 1348 2446 1348 Size: 1098 4573 PciI A'CATG_T Cuts at: 4570 4570 Size: 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5671 PfoI T'CCnGG_A Cuts at: 2367 2367 Size: 5671 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2724 1613 Size: 1111 4560 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PspOMI G'GGCC_C Cuts at: 2441 2441 Size: 5671 PstI C_TGCA'G Cuts at: 830 2046 2196 3023 830 Size: 1216 150 827 3478 Fragments arranged by size: 3478 1216 827 150 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 PvuII CAG'CTG Cuts at: 2059 2588 2656 2059 Size: 529 68 5074 Fragments arranged by size: 5074 529 68 RsrII CG'GwC_CG Cuts at: 2365 2365 Size: 5671 SacI G_AGCT'C Cuts at: 2910 2910 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SapI GCTCTTCn'nnn_ Cuts at: 2400 2716 2751 2400 Size: 316 35 5320 Fragments arranged by size: 5320 316 35 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SfcI C'TryA_G Cuts at: 826 1045 2042 2192 3019 4154 4835 5026 826 Size: 219 997 150 827 1135 681 191 1471 Fragments arranged by size: 1471 1135 997 827 681 219 191 150 SfiI GGCCn_nnn'nGGCC Cuts at: 2117 2117 Size: 5671 SfoI GGC'GCC Cuts at: 2146 2203 2146 Size: 57 5614 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2330 2998 1872 Size: 458 668 4545 Fragments arranged by size: 4545 668 458 SmlI C'TyrA_G Cuts at: 820 1017 1943 2462 3775 4676 4938 820 Size: 197 926 519 1313 901 262 1553 Fragments arranged by size: 1553 1313 926 901 519 262 197 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 2111 1517 Size: 594 5077 StyI C'CwwG_G Cuts at: 513 1065 2565 2677 3110 513 Size: 552 1500 112 433 3074 Fragments arranged by size: 3074 1500 552 433 112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 3698 3715 3868 4053 4498 325 Size: 1255 1215 903 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1215 903 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5629 96 Size: 274 80 33 51 1443 1940 1712 138 Fragments arranged by size: 1940 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5631 5663 5631 Size: 32 5639 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2161 2161 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 2462 1943 Size: 519 5152 XmaI C'CCGG_G Cuts at: 1870 2328 2996 1870 Size: 458 668 4545 Fragments arranged by size: 4545 668 458 XmnI GAAnn'nnTTC Cuts at: 3800 3800 Size: 5671 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI SacII SanDI SexAI SrfI SwaI