KRT82 ORF clone pFN21AE0381

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Halotag ORF Clone pFN21AE0381

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Clone info.

Species

human

Clone Name

pFN21AE0381 Sequence (with vector) Restriction Map

Product ID

FHC05785

Flexi Clone PID

FXC05785

ORF Sequence

DNA sequence (1539 bp)
Protein sequence (513 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527176]

Gene

KIEE0381

Symbol

KRT82

Alias : HB2, Hb-2, KRTHB2

Description

keratin 82, type II

Original Clone ID

oc01081

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1539 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 513 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8826.1 2.5e-152 100.0 KRT82 CCDS8824.1 3.3e-82 60.7 KRT85 CCDS8825.1 5.3e-79 60.8 KRT84 CCDS31805.1 3.4e-76 59.6 KRT81 CCDS41785.1 2.2e-75 60.6 KRT86 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003054 122 130 PR01276 Type II keratin IPR003054 149 161 PR01276 Type II keratin IPR003054 194 207 PR01276 Type II keratin IPR003054 208 227 PR01276 Type II keratin IPR003054 319 336 PR01276 Type II keratin IPR003054 370 385 PR01276 Type II keratin HMMPfam IPR016044 120 430 PF00038 Filament
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_149022 513 601078 100.0 100.0 NP_002274 507 602032,602767 60.7 96.7 XP_011536637 600 602766 60.8 94.7 NP_149034 600 602766 60.8 94.7 NP_002272 505 158000,602153 59.6 97.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0381 Download

>KIEE0381  1539 bp
ATGTCGTACCACTCTTTCCAGCCAGGCTCCAGGTGTGGCAGTCAGAGTTTCAGCTCATAC
TCGGCTGTCATGCCCCGGATGGTCACCCACTATGCAGTGAGCAAGGGGCCATGCCGGCCC
GGGGGTGGTAGGGGCCTCCGAGCTCTGGGCTGCCTTGGCTCACGGAGCCTGTGCAACGTG
GGCTTTGGGAGGCCCCGGGTAGCCTCCAGGTGTGGAGGTACCCTGCCTGGCTTCGGGTAC
CGACTGGGAGCCACCTGTGGGCCTTCTGCCTGCATCACCCCTGTCACCATCAATGAGAGC
CTGCTGGTCCCACTGGCACTGGAGATAGACCCGACTGTGCAGAGGGTAAAGAGGGATGAG
AAGGAGCAGATCAAGTGCCTCAACAACCGTTTCGCATCTTTCATCAACAAGGTCCGTTTC
CTGGAGCAGAAGAACAAGCTGCTGGAGACCAAGTGGAACTTCATGCAGCAGCAGAGGTGC
TGCCAGACCAACATCGAGCCCATCTTCGAGGGCTATATCAGCGCCCTTCGGCGGCAGCTG
GACTGTGTGTCCGGGGACCGCGTGAGGCTAGAGTCAGAGCTCTGCAGCCTCCAGGCTGCA
CTGGAGGGCTACAAGAAAAAATACGAAGAGGAGCTCTCCCTGCGTCCCTGTGTTGAGAAT
GAGTTTGTTGCCTTGAAGAAGGACGTGGACACAGCCTTCCTGATGAAGGCTGACCTGGAG
ACCAACGCAGAGGCACTCGTGCAGGAGATCGACTTCCTGAAAAGCCTGTATGAGGAGGAG
ATCTGCCTGCTCCAGTCTCAGATCTCTGAGACCTCGGTCATTGTGAAGATGGACAACAGC
CGGGAGCTGGACGTGGACGGCATCATCGCTGAGATCAAGGCGCAGTATGACGACATCGCC
AGCCGCAGCAAAGCCGAAGCAGAGGCCTGGTACCAGTGCCGGTATGAGGAGCTGAGAGTC
ACAGCTGGGAACCACTGTGACAACCTCCGCAACCGTAAGAACGAGATCCTGGAAATGAAT
AAACTGATCCAGCGGCTGCAGCAAGAAACCGAGAATGTCAAAGCCCAGCGCTGCAAACTT
GAGGGTGCCATAGCTGAGGCAGAGCAGCAGGGCGAGGCGGCTCTCAATGATGCCAAGTGC
AAGCTGGCAGGGCTGGAGGAGGCTCTGCAGAAGGCCAAGCAGGACATGGCCTGCCTGCTC
AAGGAATATCAGGAGGTGATGAACTCCAAGCTGGGCCTGGACATCGAGATCGCCACCTAC
AGGCGCCTGCTGGAGGGTGAAGAGCACAGGCTGTGCGAAGGCATCGGGCCCGTGAATATC
TCAGTGAGCAGCTCCAAAGGCGCCTTCCTGTACGAGCCATGTGGGGTCAGCACGCCTGTC
CTCAGCACTGGCGTCCTCAGGAGCAATGGGGGCTGCAGCATCGTGGGCACTGGTGAACTC
TATGTCCCCTGCGAGCCCCAGGGGCTACTGAGCTGTGGGAGCGGGCGGAAATCCAGCATG
ACGCTAGGAGCTGGGGGCAGCTCCCCCAGCCACAAGCAT

ORF Protein

Cloned ORF protein sequence for pFN21AE0381 Download

>KIEE0381  513 aa
MSYHSFQPGSRCGSQSFSSYSAVMPRMVTHYAVSKGPCRPGGGRGLRALGCLGSRSLCNV
GFGRPRVASRCGGTLPGFGYRLGATCGPSACITPVTINESLLVPLALEIDPTVQRVKRDE
KEQIKCLNNRFASFINKVRFLEQKNKLLETKWNFMQQQRCCQTNIEPIFEGYISALRRQL
DCVSGDRVRLESELCSLQAALEGYKKKYEEELSLRPCVENEFVALKKDVDTAFLMKADLE
TNAEALVQEIDFLKSLYEEEICLLQSQISETSVIVKMDNSRELDVDGIIAEIKAQYDDIA
SRSKAEAEAWYQCRYEELRVTAGNHCDNLRNRKNEILEMNKLIQRLQQETENVKAQRCKL
EGAIAEAEQQGEAALNDAKCKLAGLEEALQKAKQDMACLLKEYQEVMNSKLGLDIEIATY
RRLLEGEEHRLCEGIGPVNISVSSSKGAFLYEPCGVSTPVLSTGVLRSNGGCSIVGTGEL
YVPCEPQGLLSCGSGRKSSMTLGAGGSSPSHKH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0381 Download

>pFN21AE0381  6244 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGTACCACTCTTTCCAGCCAGGCTCCAGGTG
TGGCAGTCAGAGTTTCAGCTCATACTCGGCTGTCATGCCCCGGATGGTCACCCACTATGC
AGTGAGCAAGGGGCCATGCCGGCCCGGGGGTGGTAGGGGCCTCCGAGCTCTGGGCTGCCT
TGGCTCACGGAGCCTGTGCAACGTGGGCTTTGGGAGGCCCCGGGTAGCCTCCAGGTGTGG
AGGTACCCTGCCTGGCTTCGGGTACCGACTGGGAGCCACCTGTGGGCCTTCTGCCTGCAT
CACCCCTGTCACCATCAATGAGAGCCTGCTGGTCCCACTGGCACTGGAGATAGACCCGAC
TGTGCAGAGGGTAAAGAGGGATGAGAAGGAGCAGATCAAGTGCCTCAACAACCGTTTCGC
ATCTTTCATCAACAAGGTCCGTTTCCTGGAGCAGAAGAACAAGCTGCTGGAGACCAAGTG
GAACTTCATGCAGCAGCAGAGGTGCTGCCAGACCAACATCGAGCCCATCTTCGAGGGCTA
TATCAGCGCCCTTCGGCGGCAGCTGGACTGTGTGTCCGGGGACCGCGTGAGGCTAGAGTC
AGAGCTCTGCAGCCTCCAGGCTGCACTGGAGGGCTACAAGAAAAAATACGAAGAGGAGCT
CTCCCTGCGTCCCTGTGTTGAGAATGAGTTTGTTGCCTTGAAGAAGGACGTGGACACAGC
CTTCCTGATGAAGGCTGACCTGGAGACCAACGCAGAGGCACTCGTGCAGGAGATCGACTT
CCTGAAAAGCCTGTATGAGGAGGAGATCTGCCTGCTCCAGTCTCAGATCTCTGAGACCTC
GGTCATTGTGAAGATGGACAACAGCCGGGAGCTGGACGTGGACGGCATCATCGCTGAGAT
CAAGGCGCAGTATGACGACATCGCCAGCCGCAGCAAAGCCGAAGCAGAGGCCTGGTACCA
GTGCCGGTATGAGGAGCTGAGAGTCACAGCTGGGAACCACTGTGACAACCTCCGCAACCG
TAAGAACGAGATCCTGGAAATGAATAAACTGATCCAGCGGCTGCAGCAAGAAACCGAGAA
TGTCAAAGCCCAGCGCTGCAAACTTGAGGGTGCCATAGCTGAGGCAGAGCAGCAGGGCGA
GGCGGCTCTCAATGATGCCAAGTGCAAGCTGGCAGGGCTGGAGGAGGCTCTGCAGAAGGC
CAAGCAGGACATGGCCTGCCTGCTCAAGGAATATCAGGAGGTGATGAACTCCAAGCTGGG
CCTGGACATCGAGATCGCCACCTACAGGCGCCTGCTGGAGGGTGAAGAGCACAGGCTGTG
CGAAGGCATCGGGCCCGTGAATATCTCAGTGAGCAGCTCCAAAGGCGCCTTCCTGTACGA
GCCATGTGGGGTCAGCACGCCTGTCCTCAGCACTGGCGTCCTCAGGAGCAATGGGGGCTG
CAGCATCGTGGGCACTGGTGAACTCTATGTCCCCTGCGAGCCCCAGGGGCTACTGAGCTG
TGGGAGCGGGCGGAAATCCAGCATGACGCTAGGAGCTGGGGGCAGCTCCCCCAGCCACAA
GCATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT