(Circular) (Six-base) MAPSORT of: pFN21AE0381.seq Check: 4234 from: 1 to: 6244 pFN21AE0381 6244 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4054 278 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3587 1937 Size: 1650 4594 Acc65I G'GTAC_C Cuts at: 2222 2241 2934 3565 2222 Size: 19 693 631 4901 Fragments arranged by size: 4901 693 631 19 AclI AA'CG_TT Cuts at: 1563 4371 4744 1563 Size: 2808 373 3063 Fragments arranged by size: 3063 2808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4307 5691 5959 1632 Size: 2675 1384 268 1917 Fragments arranged by size: 2675 1917 1384 268 AfeI AGC'GCT Cuts at: 3074 3074 Size: 6244 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6047 AflIII A'CryG_T Cuts at: 1203 5143 6120 1203 Size: 3940 977 1327 Fragments arranged by size: 3940 1327 977 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 2551 4973 2551 Size: 2422 3822 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6244 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6244 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3432 3464 3432 Size: 32 6212 AlwNI CAG_nnn'CTG Cuts at: 3040 3987 5559 3040 Size: 947 1572 3725 Fragments arranged by size: 3725 1572 947 ApaI G_GGCC'C Cuts at: 3315 3315 Size: 6244 ApaLI G'TGCA_C Cuts at: 4301 5457 4301 Size: 1156 5088 ApoI r'AATT_y Cuts at: 1493 3553 3779 3931 6040 1493 Size: 2060 226 152 2109 1697 Fragments arranged by size: 2109 2060 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2199 3569 1870 Size: 73 180 76 1370 4545 Fragments arranged by size: 4545 1370 180 76 73 BamHI G'GATC_C Cuts at: 3574 3941 3574 Size: 367 5877 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2222 2241 2934 3089 3267 Size: 325 217 627 435 19 693 155 178 Cuts at: 3267 3344 3565 5020 618 Size: 77 221 1455 1842 Fragments arranged by size: 1842 1455 693 627 435 325 221 217 178 155 77 19 BanII G_rGCy'C Cuts at: 1391 2149 2505 2586 2640 3315 3462 3563 1391 Size: 758 356 81 54 675 147 101 4072 Fragments arranged by size: 4072 758 675 356 147 101 81 54 BbeI G_GCGC'C Cuts at: 3271 3348 3271 Size: 77 6167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5273 BbvCI CC'TCA_GC Cuts at: 3099 3386 3099 Size: 287 5957 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4435 4469 1903 Size: 34 2498 34 3678 Fragments arranged by size: 3678 2498 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4136 5346 4136 Size: 1210 5034 BclI T'GATC_A Cuts at: 1548 1548 Size: 6244 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2119 3466 4855 Size: 107 122 71 631 605 447 1347 1389 Cuts at: 4855 136 Size: 1525 Fragments arranged by size: 1525 1389 1347 631 605 447 122 107 71 BglII A'GATC_T Cuts at: 2784 2805 6239 2784 Size: 21 3434 2789 Fragments arranged by size: 3434 2789 21 BlpI GC'TnA_GC Cuts at: 3661 3661 Size: 6244 Bme1580I G_kGCm'C Cuts at: 1790 3315 3434 4305 5461 1790 Size: 1525 119 871 1156 2573 Fragments arranged by size: 2573 1525 1156 871 119 BmgBI CAC'GTC Cuts at: 1318 2689 2857 1318 Size: 1371 168 4705 Fragments arranged by size: 4705 1371 168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2258 3975 4933 Size: 416 53 83 399 24 841 1717 958 Cuts at: 4933 442 Size: 1753 Fragments arranged by size: 1753 1717 958 841 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2656 2656 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2017 2194 2345 2447 2468 2579 2627 2741 Size: 778 177 151 102 21 111 48 114 Cuts at: 2741 2780 3179 3296 4904 1239 Size: 39 399 117 1608 2579 Fragments arranged by size: 2579 1608 778 399 177 151 117 114 111 102 48 39 21 Bpu10I CC'TnA_GC Cuts at: 3099 3386 3099 Size: 287 5957 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3104 3188 4369 5234 5532 3104 Size: 84 1181 865 298 3816 Fragments arranged by size: 3816 1181 865 298 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2444 2717 2807 4907 882 Size: 617 945 273 90 2100 2219 Fragments arranged by size: 2219 2100 945 617 273 90 BsaAI yAC'GTr Cuts at: 493 1204 4035 493 Size: 711 2831 2702 Fragments arranged by size: 2831 2702 711 BsaBI GATnn'nnATC Cuts at: 6238 6238 Size: 6244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3268 3345 3396 4051 Size: 53 83 186 936 1735 77 51 655 Cuts at: 4051 4433 5068 275 Size: 382 635 1451 Fragments arranged by size: 1735 1451 936 655 635 382 186 83 77 53 51 BsaWI w'CCGG_w Cuts at: 4677 5075 5349 5496 5907 4677 Size: 398 274 147 411 5014 Fragments arranged by size: 5014 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 1178 Size: 30 165 30 1038 30 4951 Fragments arranged by size: 4951 1038 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2648 2792 2795 2966 3176 1186 Size: 1462 144 3 171 210 4254 Fragments arranged by size: 4254 1462 210 171 144 3 BseYI C'CCAG_C Cuts at: 2969 3069 3235 3515 3530 5447 5943 2969 Size: 100 166 280 15 1917 496 3270 Fragments arranged by size: 3270 1917 496 280 166 100 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2363 2586 2765 1163 Size: 573 627 223 179 4642 Fragments arranged by size: 4642 627 573 223 179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3694 4455 5483 1540 Size: 275 186 1693 761 1028 2301 Fragments arranged by size: 2301 1693 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2149 2586 2640 3291 4305 4390 5461 2149 Size: 437 54 651 1014 85 1071 2932 Fragments arranged by size: 2932 1071 1014 651 437 85 54 BsmI GAATG_Cn' Cuts at: 3755 3848 3755 Size: 93 6151 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2149 2505 2586 2640 3291 3315 3434 Size: 399 359 356 81 54 651 24 119 Cuts at: 3434 3462 3563 4305 4390 5461 1391 Size: 28 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 399 359 356 119 101 85 81 54 28 24 BspEI T'CCGG_A Cuts at: 5907 5907 Size: 6244 BspHI T'CATG_A Cuts at: 4133 5863 4133 Size: 1730 4514 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3599 844 Size: 2755 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3486 4131 1394 Size: 429 1663 645 3507 Fragments arranged by size: 3507 1663 645 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3415 4739 4913 65 Size: 1183 2167 1324 174 1396 Fragments arranged by size: 2167 1396 1324 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2118 2943 4888 5075 1700 Size: 110 308 825 1945 187 2869 Fragments arranged by size: 2869 1945 825 308 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6244 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6244 BssSI C'ACGA_G Cuts at: 2741 4304 5316 2741 Size: 1563 1012 3669 Fragments arranged by size: 3669 1563 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6244 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 2086 1184 Size: 902 5342 BstYI r'GATC_y Cuts at: 1972 2784 2805 3009 3574 3941 4326 4343 5795 Size: 812 21 204 565 367 385 17 1452 Cuts at: 5795 5881 5893 6239 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 812 565 385 367 346 204 86 21 17 12 Bsu36I CC'TnA_GG Cuts at: 3401 3401 Size: 6244 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5692 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2854 2884 487 Size: 683 56 1628 30 3847 Fragments arranged by size: 3847 1628 683 56 30 BtsI GCAGTG_nn' Cuts at: 2106 3760 4554 4574 2106 Size: 1654 794 20 3776 Fragments arranged by size: 3776 1654 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6244 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 3548 3548 Size: 6244 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5251 809 Size: 507 3935 1802 Fragments arranged by size: 3935 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3691 4580 6209 Size: 54 1425 50 275 18 1861 889 1629 Cuts at: 6209 8 Size: 43 Fragments arranged by size: 1861 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3691 1537 Size: 275 1879 4090 Fragments arranged by size: 4090 1879 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2625 3279 4174 1353 Size: 1272 654 895 3423 Fragments arranged by size: 3423 1272 895 654 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3505 4831 5205 5351 387 Size: 1064 138 1916 1326 374 146 1280 Fragments arranged by size: 1916 1326 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2147 2584 2638 2147 Size: 437 54 5753 Fragments arranged by size: 5753 437 54 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2017 2194 2345 2447 2468 2579 2627 Size: 393 385 177 151 102 21 111 48 Cuts at: 2627 2741 2780 3179 3296 4307 4904 5691 5959 Size: 114 39 399 117 1011 597 787 268 Cuts at: 5959 1239 Size: 1524 Fragments arranged by size: 1524 1011 787 597 399 393 385 268 177 151 117 114 111 102 48 39 21 EcoNI CCTnn'n_nnAGG Cuts at: 2708 2774 3203 3275 2708 Size: 66 429 72 5677 Fragments arranged by size: 5677 429 72 66 EcoO109I rG'GnC_Cy Cuts at: 2137 2196 2137 Size: 59 6185 EcoRI G'AATT_C Cuts at: 3553 3931 3553 Size: 378 5866 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2479 2479 Size: 6244 FspI TGC'GCA Cuts at: 4750 4750 Size: 6244 HaeII r_GCGC'y Cuts at: 1678 2529 3076 3271 3348 5391 1678 Size: 851 547 195 77 2043 2531 Fragments arranged by size: 2531 2043 851 547 195 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 4885 4917 4959 Size: 32 135 32 965 32 2316 32 42 Cuts at: 4959 4991 1373 Size: 32 2626 Fragments arranged by size: 2626 2316 965 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3588 3834 669 Size: 988 281 1650 246 3079 Fragments arranged by size: 3079 1650 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6244 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KasI G'GCGC_C Cuts at: 3267 3344 3267 Size: 77 6167 KpnI G_GTAC'C Cuts at: 2226 2245 2938 3569 2226 Size: 19 693 631 4901 Fragments arranged by size: 4901 693 631 19 MfeI C'AATT_G Cuts at: 3843 3843 Size: 6244 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5358 5542 1305 Size: 4053 184 2007 Fragments arranged by size: 4053 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4422 Fragments arranged by size: 4422 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3540 4202 4561 4720 518 Size: 648 2374 662 359 159 2042 Fragments arranged by size: 2374 2042 662 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2542 2969 3037 3660 4337 5485 5730 1606 Size: 936 427 68 623 677 1148 245 2120 Fragments arranged by size: 2120 1148 936 677 623 427 245 68 NaeI GCC'GGC Cuts at: 2120 2120 Size: 6244 NarI GG'CG_CC Cuts at: 3268 3345 3268 Size: 77 6167 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5692 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NgoMIV G'CCGG_C Cuts at: 2118 2118 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 3691 3691 Size: 6244 NspI r_CATG'y Cuts at: 1132 1229 3545 3602 5147 1132 Size: 97 2316 57 1545 2229 Fragments arranged by size: 2316 2229 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 3463 1348 Size: 2115 4129 PciI A'CATG_T Cuts at: 5143 5143 Size: 6244 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6244 PfoI T'CCnGG_A Cuts at: 2424 3012 2424 Size: 588 5656 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4313 4345 5826 5858 4313 Size: 32 1481 32 4699 Fragments arranged by size: 4699 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2819 1613 Size: 1206 5038 PsiI TTA'TAA Cuts at: 3814 3814 Size: 6244 PspOMI G'GGCC_C Cuts at: 3311 3311 Size: 6244 PstI C_TGCA'G Cuts at: 830 2591 3045 3174 3422 3596 830 Size: 1761 454 129 248 174 3478 Fragments arranged by size: 3478 1761 454 248 174 129 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 PvuII CAG'CTG Cuts at: 2542 2969 2542 Size: 427 5817 SacI G_AGCT'C Cuts at: 2149 2586 2640 2149 Size: 437 54 5753 Fragments arranged by size: 5753 437 54 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SapI GCTCTTCn'nnn_ Cuts at: 3279 3279 Size: 6244 SbfI CC_TGCA'GG Cuts at: 3596 3596 Size: 6244 ScaI AGT'ACT Cuts at: 4492 6204 4492 Size: 1712 4532 SfcI C'TryA_G Cuts at: 826 1045 2587 3041 3170 3262 3418 3592 4727 Size: 219 1542 454 129 92 156 174 1135 Cuts at: 4727 5408 5599 826 Size: 681 191 1471 Fragments arranged by size: 1542 1471 1135 681 454 219 191 174 156 129 92 SfiI GGCCn_nnn'nGGCC Cuts at: 2119 2119 Size: 6244 SfoI GGC'GCC Cuts at: 3269 3346 3269 Size: 77 6167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 2125 2201 3571 1872 Size: 253 76 1370 4545 Fragments arranged by size: 4545 1370 253 76 SmlI C'TyrA_G Cuts at: 820 1017 1943 3083 3203 4348 5249 5511 820 Size: 197 926 1140 120 1145 901 262 1553 Fragments arranged by size: 1553 1145 1140 926 901 262 197 120 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 4168 5 Size: 47 4116 2081 Fragments arranged by size: 4116 2081 47 StuI AGG'CCT Cuts at: 1517 2930 1517 Size: 1413 4831 StyI C'CwwG_G Cuts at: 513 1065 2158 3683 513 Size: 552 1093 1525 3074 Fragments arranged by size: 3074 1525 1093 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 2809 4271 4288 4441 4626 5071 Size: 1255 525 704 1462 17 153 185 445 Cuts at: 5071 325 Size: 1498 Fragments arranged by size: 1498 1462 1255 704 525 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4490 6202 96 Size: 274 80 33 51 1443 2513 1712 138 Fragments arranged by size: 2513 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2441 2473 2965 2997 6204 6236 2441 Size: 32 492 32 3207 32 2449 Fragments arranged by size: 3207 2449 492 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6244 XbaI T'CTAG_A Cuts at: 3580 3580 Size: 6244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3145 3187 3238 3145 Size: 42 51 6151 Fragments arranged by size: 6151 51 42 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6244 XmaI C'CCGG_G Cuts at: 1870 2123 2199 3569 1870 Size: 253 76 1370 4545 Fragments arranged by size: 4545 1370 253 76 XmnI GAAnn'nnTTC Cuts at: 4373 4373 Size: 6244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4052 276 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI