ALG12 ORF clone pFN21AE0336

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Halotag ORF Clone pFN21AE0336

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Clone info.

Species

human

Clone Name

pFN21AE0336 Sequence (with vector) Restriction Map

Product ID

FHC04083

Flexi Clone PID

FXC04083

ORF Sequence

DNA sequence (1464 bp)
Protein sequence (488 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527103]

Gene

KIEE0336

Symbol

ALG12

Alias : CDG1G, ECM39, PP14673, hALG12

Description

ALG12, alpha-1,6-mannosyltransferase

Original Clone ID

oc01036

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1464 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 488 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14081.1 0 100.0 ALG12 CCDS61641.1 8.3e-15 26.2 PIGB The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR005599 19 382 PF03901 GPI mannosyltransferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 11 PLLLGLLVAVATVHLVICPYTKV 33 PRIMARY 23 2 69 LGPVVIAVFSSPAVYVLSLLEMS 91 PRIMARY 23 3 94 YSQLIVRGVLGLGVIFGLWTLQK 116 PRIMARY 23 4 121 HFGAMVATMFCWVTAMQFHLMFY 143 SECONDARY 23 5 147 TLPNVLALPVVLLALAAWLRHEW 169 PRIMARY 23 6 174 WLSAFAIIVFRVELCLFLGLLLL 196 PRIMARY 23 7 212 HAVPAGILCLGLTVAVDSYFWRQ 234 PRIMARY 23 8 354 VNAAYSATALYVSHFNYPGGVAM 376 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_077010 488 607143,607144 100.0 100.0 XP_016884426 441 607143,607144 99.3 85.2 XP_016884425 447 607143,607144 95.7 89.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0336 Download

>KIEE0336  1464 bp
ATGGCTGGAAAGGGGTCATCAGGCAGGCGGCCCCTGCTGCTGGGGCTGCTGGTGGCCGTA
GCCACTGTCCACCTGGTCATCTGTCCCTACACCAAAGTGGAGGAGAGCTTCAACCTGCAG
GCCACACATGACCTGCTCTACCACTGGCAAGACCTGGAGCAGTACGACCATCTTGAGTTC
CCCGGAGTCGTCCCCAGGACGTTCCTCGGGCCAGTGGTGATCGCAGTGTTCTCCAGCCCC
GCGGTTTACGTGCTTTCGCTGTTAGAAATGTCCAAGTTTTACTCTCAGCTAATAGTTAGA
GGAGTGCTTGGACTCGGCGTGATTTTTGGACTCTGGACGTTACAAAAGGAAGTGAGACGG
CACTTCGGGGCCATGGTGGCCACCATGTTCTGCTGGGTGACGGCCATGCAGTTCCACCTG
ATGTTCTACTGCACGCGGACACTGCCCAATGTGCTGGCCCTGCCTGTAGTCCTGCTGGCC
CTCGCGGCCTGGCTGCGGCACGAGTGGGCCCGCTTCATCTGGCTGTCAGCCTTCGCCATC
ATCGTGTTCAGGGTGGAGCTGTGCCTGTTCCTGGGCCTCCTGCTGCTGCTGGCCTTGGGC
AACCGAAAGGTTTCTGTAGTCAGAGCCCTTCGCCACGCCGTCCCGGCAGGGATCCTCTGT
TTAGGACTGACGGTTGCTGTGGACTCTTATTTTTGGCGGCAGCTCACTTGGCCGGAAGGA
AAGGTGCTTTGGTACAACACTGTCCTGAACAAAAGCTCCAACTGGGGGACCTCCCCGCTG
CTGTGGTACTTCTACTCAGCCCTGCCCCGCGGCCTGGGCTGCAGCCTGCTCTTCATCCCC
CTGGGCTTGGTAGACAGAAGGACGCACGCGCCGACGGTGCTGGCACTGGGCTTCATGGCA
CTCTACTCCCTCCTGCCACACAAGGAGCTACGCTTCATCATCTATGCCTTCCCCATGCTC
AACATCACGGCTGCCAGAGGCTGCTCCTACCTGCTGAATAACTATAAAAAGTCTTGGCTG
TACAAAGCGGGGTCTCTGCTTGTGATCGGACACCTCGTGGTGAATGCCGCCTACTCAGCC
ACGGCCCTGTATGTGTCCCATTTCAACTACCCAGGTGGCGTCGCAATGCAGAGGCTGCAC
CAGCTGGTGCCCCCCCAGACAGACGTCCTTCTGCACATTGACGTGGCAGCCGCCCAGACA
GGTGTGTCTCGGTTTCTCCAAGTCAACAGCGCCTGGAGGTACGACAAGAGGGAGGATGTG
CAGCCGGGGACAGGCATGCTGGCATACACACACATCCTCATGGAGGCGGCCCCTGGGCTC
CTGGCCCTCTACAGGGACACACACCGGGTCCTGGCCAGCGTCGTGGGGACCACAGGTGTG
AGTCTGAACCTGACCCAACTGCCCCCCTTCAACGTCCACCTGCAGACAAAGCTGGTGCTT
CTGGAGAGGCTCCCCCGGCCGTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE0336 Download

>KIEE0336  488 aa
MAGKGSSGRRPLLLGLLVAVATVHLVICPYTKVEESFNLQATHDLLYHWQDLEQYDHLEF
PGVVPRTFLGPVVIAVFSSPAVYVLSLLEMSKFYSQLIVRGVLGLGVIFGLWTLQKEVRR
HFGAMVATMFCWVTAMQFHLMFYCTRTLPNVLALPVVLLALAAWLRHEWARFIWLSAFAI
IVFRVELCLFLGLLLLLALGNRKVSVVRALRHAVPAGILCLGLTVAVDSYFWRQLTWPEG
KVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFIPLGLVDRRTHAPTVLALGFMA
LYSLLPHKELRFIIYAFPMLNITAARGCSYLLNNYKKSWLYKAGSLLVIGHLVVNAAYSA
TALYVSHFNYPGGVAMQRLHQLVPPQTDVLLHIDVAAAQTGVSRFLQVNSAWRYDKREDV
QPGTGMLAYTHILMEAAPGLLALYRDTHRVLASVVGTTGVSLNLTQLPPFNVHLQTKLVL
LERLPRPS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0336 Download

>pFN21AE0336  6169 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGGAAAGGGGTCATCAGGCAGGCGGCCCCT
GCTGCTGGGGCTGCTGGTGGCCGTAGCCACTGTCCACCTGGTCATCTGTCCCTACACCAA
AGTGGAGGAGAGCTTCAACCTGCAGGCCACACATGACCTGCTCTACCACTGGCAAGACCT
GGAGCAGTACGACCATCTTGAGTTCCCCGGAGTCGTCCCCAGGACGTTCCTCGGGCCAGT
GGTGATCGCAGTGTTCTCCAGCCCCGCGGTTTACGTGCTTTCGCTGTTAGAAATGTCCAA
GTTTTACTCTCAGCTAATAGTTAGAGGAGTGCTTGGACTCGGCGTGATTTTTGGACTCTG
GACGTTACAAAAGGAAGTGAGACGGCACTTCGGGGCCATGGTGGCCACCATGTTCTGCTG
GGTGACGGCCATGCAGTTCCACCTGATGTTCTACTGCACGCGGACACTGCCCAATGTGCT
GGCCCTGCCTGTAGTCCTGCTGGCCCTCGCGGCCTGGCTGCGGCACGAGTGGGCCCGCTT
CATCTGGCTGTCAGCCTTCGCCATCATCGTGTTCAGGGTGGAGCTGTGCCTGTTCCTGGG
CCTCCTGCTGCTGCTGGCCTTGGGCAACCGAAAGGTTTCTGTAGTCAGAGCCCTTCGCCA
CGCCGTCCCGGCAGGGATCCTCTGTTTAGGACTGACGGTTGCTGTGGACTCTTATTTTTG
GCGGCAGCTCACTTGGCCGGAAGGAAAGGTGCTTTGGTACAACACTGTCCTGAACAAAAG
CTCCAACTGGGGGACCTCCCCGCTGCTGTGGTACTTCTACTCAGCCCTGCCCCGCGGCCT
GGGCTGCAGCCTGCTCTTCATCCCCCTGGGCTTGGTAGACAGAAGGACGCACGCGCCGAC
GGTGCTGGCACTGGGCTTCATGGCACTCTACTCCCTCCTGCCACACAAGGAGCTACGCTT
CATCATCTATGCCTTCCCCATGCTCAACATCACGGCTGCCAGAGGCTGCTCCTACCTGCT
GAATAACTATAAAAAGTCTTGGCTGTACAAAGCGGGGTCTCTGCTTGTGATCGGACACCT
CGTGGTGAATGCCGCCTACTCAGCCACGGCCCTGTATGTGTCCCATTTCAACTACCCAGG
TGGCGTCGCAATGCAGAGGCTGCACCAGCTGGTGCCCCCCCAGACAGACGTCCTTCTGCA
CATTGACGTGGCAGCCGCCCAGACAGGTGTGTCTCGGTTTCTCCAAGTCAACAGCGCCTG
GAGGTACGACAAGAGGGAGGATGTGCAGCCGGGGACAGGCATGCTGGCATACACACACAT
CCTCATGGAGGCGGCCCCTGGGCTCCTGGCCCTCTACAGGGACACACACCGGGTCCTGGC
CAGCGTCGTGGGGACCACAGGTGTGAGTCTGAACCTGACCCAACTGCCCCCCTTCAACGT
CCACCTGCAGACAAAGCTGGTGCTTCTGGAGAGGCTCCCCCGGCCGTCCGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT