(Circular) (Six-base) MAPSORT of: pFN21AE0336.seq Check: 1720 from: 1 to: 6169 pFN21AE0336 6169 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3432 3432 Size: 6169 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3171 3979 278 Size: 53 83 186 936 1635 808 2468 Fragments arranged by size: 2468 1635 936 808 186 83 53 AccI GT'mk_AC Cuts at: 1937 2856 3512 1937 Size: 919 656 4594 Fragments arranged by size: 4594 919 656 Acc65I G'GTAC_C Cuts at: 3490 3490 Size: 6169 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4232 5616 5884 1632 Size: 2600 1384 268 1917 Fragments arranged by size: 2600 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5972 AflIII A'CryG_T Cuts at: 1203 5068 6045 1203 Size: 3865 977 1327 Fragments arranged by size: 3865 1327 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 4898 4898 Size: 6169 AleI CACnn'nnGTG Cuts at: 1166 2099 3380 1166 Size: 933 1281 3955 Fragments arranged by size: 3955 1281 933 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6169 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2744 2776 2744 Size: 32 6137 AlwNI CAG_nnn'CTG Cuts at: 2985 3139 3237 3912 5484 2985 Size: 154 98 675 1572 3670 Fragments arranged by size: 3670 1572 675 154 98 ApaI G_GGCC'C Cuts at: 2515 2515 Size: 6169 ApaLI G'TGCA_C Cuts at: 4226 5382 4226 Size: 1156 5013 ApoI r'AATT_y Cuts at: 1493 3478 3704 3856 5965 1493 Size: 1985 226 152 2109 1697 Fragments arranged by size: 2109 1985 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 2210 3494 1870 Size: 73 267 1284 4545 Fragments arranged by size: 4545 1284 267 73 BamHI G'GATC_C Cuts at: 2655 3499 3866 2655 Size: 844 367 4958 Fragments arranged by size: 4958 844 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3151 3490 4945 618 Size: 325 217 627 1364 339 1455 1842 Fragments arranged by size: 1842 1455 1364 627 339 325 217 BanII G_rGCy'C Cuts at: 1391 2515 2632 3324 3488 1391 Size: 1124 117 692 164 4072 Fragments arranged by size: 4072 1124 692 164 117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4360 4394 1903 Size: 34 2423 34 3678 Fragments arranged by size: 3678 2423 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 1548 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2381 2489 2648 Size: 107 122 71 631 605 709 108 159 Cuts at: 2648 4780 136 Size: 2132 1525 Fragments arranged by size: 2132 1525 709 631 605 159 122 108 107 71 BglII A'GATC_T Cuts at: 6164 6164 Size: 6169 BlpI GC'TnA_GC Cuts at: 3586 3586 Size: 6169 Bme1580I G_kGCm'C Cuts at: 1790 2515 3156 4230 5386 1790 Size: 725 641 1074 1156 2573 Fragments arranged by size: 2573 1156 1074 725 641 BmgBI CAC'GTC Cuts at: 1318 3187 1318 Size: 1869 4300 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2776 2900 3900 Size: 416 53 83 399 24 1359 124 1000 Cuts at: 3900 4858 442 Size: 958 1753 Fragments arranged by size: 1753 1359 1000 958 416 399 124 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2221 3259 3467 4829 1239 Size: 941 41 1038 208 1362 2579 Fragments arranged by size: 2579 1362 1038 941 208 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2198 4294 5159 5457 2198 Size: 2096 865 298 2910 Fragments arranged by size: 2910 2096 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3042 4832 882 Size: 617 1543 1790 2219 Fragments arranged by size: 2219 1790 1543 617 BsaAI yAC'GTr Cuts at: 493 1204 2254 3960 493 Size: 711 1050 1706 2702 Fragments arranged by size: 2702 1706 1050 711 BsaBI GATnn'nnATC Cuts at: 6163 6163 Size: 6169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3123 3168 3976 4358 Size: 53 83 186 936 1590 45 808 382 Cuts at: 4358 4993 275 Size: 635 1451 Fragments arranged by size: 1590 1451 936 808 635 382 186 83 53 45 BsaWI w'CCGG_w Cuts at: 4602 5000 5274 5421 5832 4602 Size: 398 274 147 411 4939 Fragments arranged by size: 4939 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2744 2774 1178 Size: 30 165 30 1341 30 4573 Fragments arranged by size: 4573 1341 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2319 1186 Size: 934 199 5036 Fragments arranged by size: 5036 934 199 BseYI C'CCAG_C Cuts at: 2044 2397 5372 5868 2044 Size: 353 2975 496 2345 Fragments arranged by size: 2975 2345 496 353 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2419 3125 3161 3284 1163 Size: 573 683 706 36 123 4048 Fragments arranged by size: 4048 706 683 573 123 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3464 3619 4380 5408 1540 Size: 275 186 1463 155 761 1028 2301 Fragments arranged by size: 2301 1463 1028 761 275 186 155 BsiHKAI G_wGCw'C Cuts at: 4230 4315 5386 4230 Size: 85 1071 5013 Fragments arranged by size: 5013 1071 85 BsmI GAATG_Cn' Cuts at: 3073 3680 3773 3073 Size: 607 93 5469 Fragments arranged by size: 5469 607 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2353 2353 Size: 6169 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2515 2632 3156 3324 3488 4230 4315 Size: 399 725 117 524 168 164 742 85 Cuts at: 4315 5386 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 725 524 399 168 164 117 85 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 4058 5788 4058 Size: 1730 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 2145 3003 3432 3524 844 Size: 1283 18 858 429 92 3489 Fragments arranged by size: 3489 1283 858 429 92 18 BsrBI CCG'CTC Cuts at: 1394 1823 4056 1394 Size: 429 2233 3507 Fragments arranged by size: 3507 2233 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3135 4664 4838 65 Size: 1183 1887 1529 174 1396 Fragments arranged by size: 1887 1529 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4813 5000 1700 Size: 110 3003 187 2869 Fragments arranged by size: 3003 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3024 96 Size: 2928 3241 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6169 BssSI C'ACGA_G Cuts at: 2504 3059 4229 5241 2504 Size: 555 1170 1012 3432 Fragments arranged by size: 3432 1170 1012 555 BstAPI GCAn_nnn'nTGC Cuts at: 794 3139 794 Size: 2345 3824 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstXI CCAn_nnnn'nTGG Cuts at: 2458 3367 2458 Size: 909 5260 BstYI r'GATC_y Cuts at: 1972 2655 3499 3866 4251 4268 5720 5806 5818 Size: 683 844 367 385 17 1452 86 12 Cuts at: 5818 6164 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 844 683 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2244 2376 2812 3084 513 Size: 552 939 240 132 436 272 3598 Fragments arranged by size: 3598 939 552 436 272 240 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5430 Fragments arranged by size: 5430 683 56 BtsI GCAGTG_nn' Cuts at: 2235 2444 3685 4479 4499 2235 Size: 209 1241 794 20 3905 Fragments arranged by size: 3905 1241 794 209 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DraIII CAC_nnn'GTG Cuts at: 3061 3061 Size: 6169 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3208 5176 809 Size: 507 1892 1968 1802 Fragments arranged by size: 1968 1892 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2382 2406 Size: 54 1425 50 275 18 228 324 24 Cuts at: 2406 2714 3357 3461 3616 4505 6134 8 Size: 308 643 104 155 889 1629 43 Fragments arranged by size: 1629 1425 889 643 324 308 275 228 155 104 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3461 3616 1537 Size: 275 1649 155 4090 Fragments arranged by size: 4090 1649 275 155 EarI CTCTTCn'nnn_ Cuts at: 1353 2840 4099 1353 Size: 1487 1259 3423 Fragments arranged by size: 3423 1487 1259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4756 5130 5276 387 Size: 1064 138 3167 374 146 1280 Fragments arranged by size: 3167 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2221 3259 3467 4232 4829 5616 Size: 393 548 41 1038 208 765 597 787 Cuts at: 5616 5884 1239 Size: 268 1524 Fragments arranged by size: 1524 1038 787 765 597 548 393 268 208 41 EcoNI CCTnn'n_nnAGG Cuts at: 3305 3305 Size: 6169 EcoO109I rG'GnC_Cy Cuts at: 2772 3352 2772 Size: 580 5589 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 FspI TGC'GCA Cuts at: 4675 4675 Size: 6169 HaeII r_GCGC'y Cuts at: 1678 3237 5316 1678 Size: 1559 2079 2531 Fragments arranged by size: 2531 2079 1559 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4810 4842 4884 4916 1373 Size: 32 135 32 3238 32 42 32 2626 Fragments arranged by size: 3238 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3229 3513 3759 669 Size: 988 281 1291 284 246 3079 Fragments arranged by size: 3079 1291 988 284 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KpnI G_GTAC'C Cuts at: 3494 3494 Size: 6169 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2787 5283 5467 1305 Size: 1482 2496 184 2007 Fragments arranged by size: 2496 2007 1482 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 3359 10 Size: 54 1425 343 552 975 2820 Fragments arranged by size: 2820 1425 975 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2546 3185 3302 3380 4127 4486 Size: 648 933 447 639 117 78 747 359 Cuts at: 4486 4645 518 Size: 159 2042 Fragments arranged by size: 2042 933 747 648 639 447 359 159 117 78 MspA1I CmG'CkG Cuts at: 1606 2246 2782 2814 3148 3585 4262 5410 5655 Size: 640 536 32 334 437 677 1148 245 Cuts at: 5655 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 640 536 437 334 245 32 NcoI C'CATG_G Cuts at: 513 1065 2004 2376 513 Size: 552 939 372 4306 Fragments arranged by size: 4306 939 552 372 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 3283 3527 5072 1132 Size: 97 2054 244 1545 2229 Fragments arranged by size: 2229 2054 1545 244 97 PasI CC'CwG_GG Cuts at: 1348 2845 3317 1348 Size: 1497 472 4200 Fragments arranged by size: 4200 1497 472 PciI A'CATG_T Cuts at: 5068 5068 Size: 6169 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6169 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2744 2776 4238 4270 5751 5783 2744 Size: 32 1462 32 1481 32 3130 Fragments arranged by size: 3130 1481 1462 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2772 3352 2772 Size: 580 5589 PshAI GACnn'nnGTC Cuts at: 1613 3167 1613 Size: 1554 4615 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PspOMI G'GGCC_C Cuts at: 2511 2511 Size: 6169 PstI C_TGCA'G Cuts at: 830 2124 2828 3429 3521 830 Size: 1294 704 601 92 3478 Fragments arranged by size: 3478 1294 704 601 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 PvuII CAG'CTG Cuts at: 3148 3148 Size: 6169 SacII CC_GC'GG Cuts at: 2247 2815 2247 Size: 568 5601 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SapI GCTCTTCn'nnn_ Cuts at: 2840 2840 Size: 6169 SbfI CC_TGCA'GG Cuts at: 2124 3521 2124 Size: 1397 4772 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SexAI A'CCwGG_T Cuts at: 2076 2076 Size: 6169 SfcI C'TryA_G Cuts at: 826 1045 2120 2469 2619 2824 3334 3425 3517 Size: 219 1075 349 150 205 510 91 92 Cuts at: 3517 4652 5333 5524 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1075 681 510 349 219 205 191 150 92 91 SfiI GGCCn_nnn'nGGCC Cuts at: 2381 2489 2381 Size: 108 6061 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 3496 1872 Size: 1624 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2177 4273 5174 5436 820 Size: 197 926 234 2096 901 262 1553 Fragments arranged by size: 2096 1553 926 901 262 234 197 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 3283 3527 1132 Size: 2151 244 3774 Fragments arranged by size: 3774 2151 244 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6169 StyI C'CwwG_G Cuts at: 513 1065 2004 2376 2598 3608 513 Size: 552 939 372 222 1010 3074 Fragments arranged by size: 3074 1010 939 552 372 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2389 4196 4213 4366 4551 4996 325 Size: 1255 809 1807 17 153 185 445 1498 Fragments arranged by size: 1807 1498 1255 809 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3024 4415 6127 Size: 274 80 33 51 1443 1047 1391 1712 Cuts at: 6127 96 Size: 138 Fragments arranged by size: 1712 1443 1391 1047 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2307 2339 3045 3077 3336 3364 3368 3396 6129 Size: 32 706 32 259 28 4 28 2733 Cuts at: 6129 6161 2307 Size: 32 2315 Fragments arranged by size: 2733 2315 706 259 32 32 32 28 28 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6169 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 2074 Size: 6169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 3494 1870 Size: 1624 4545 XmnI GAAnn'nnTTC Cuts at: 2614 4298 2614 Size: 1684 4485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3169 3977 276 Size: 53 83 186 936 1635 808 2468 Fragments arranged by size: 2468 1635 936 808 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SfoI SrfI SwaI