BRS3 ORF clone pFN21AE0257

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Halotag ORF Clone pFN21AE0257

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Clone info.

Species

human

Clone Name

pFN21AE0257 Sequence (with vector) Restriction Map

Product ID

FHC10903

Flexi Clone PID

FXC10903

ORF Sequence

DNA sequence (1197 bp)
Protein sequence (399 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528791]

Gene

KIEE0257

Symbol

BRS3

Alias : BB3, BB3R, BBR3

Description

bombesin-like receptor 3

Original Clone ID

oc00957

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1197 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 399 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14656.1 1.5e-121 100.0 BRS3 CCDS14174.1 1e-57 58.6 GRPR CCDS5196.1 5.6e-53 47.8 NMBR CCDS9461.1 1.9e-23 32.9 EDNRB CCDS55902.1 2.1e-23 32.9 EDNRB The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001560 13 26 PR00637 Bombesin receptor IPR001560 26 40 PR00637 Bombesin receptor IPR000276 49 73 PR00237 GPCR IPR000276 82 103 PR00237 GPCR IPR001556 100 115 PR00358 Bombesin receptor IPR001556 121 137 PR00358 Bombesin receptor IPR000276 127 149 PR00237 GPCR IPR000276 163 184 PR00237 GPCR IPR001560 186 196 PR00637 Bombesin receptor IPR001556 206 219 PR00358 Bombesin receptor IPR000276 218 241 PR00237 GPCR IPR001556 250 268 PR00358 Bombesin receptor IPR000276 269 293 PR00237 GPCR IPR001556 303 316 PR00358 Bombesin receptor IPR000276 312 338 PR00237 GPCR IPR001556 333 346 PR00358 Bombesin receptor IPR001560 348 365 PR00637 Bombesin receptor IPR001560 365 380 PR00637 Bombesin receptor IPR001560 380 396 PR00637 Bombesin receptor HMMPfam IPR000276 64 328 PF00001 GPCR ProfileScan IPR017452 64 330 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 47 LCAIYITYAVIISVGILGNAILI 69 PRIMARY 23 2 84 IFITSLAFGDLLLLLTCVPVDAT 106 SECONDARY 23 3 120 CKVLSFIRLTSVGVSVFTLTIL 141 PRIMARY 22 4 164 TCVKAGCVWIVSMIFALPEAIFS 186 SECONDARY 23 5 216 IHSLLCFLVFYIIPLSIISVYYS 238 PRIMARY 23 6 269 IARTVLVLVALFALCWLPNHLLY 291 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001718 399 300107 100.0 100.0 NP_005305 384 305670 58.6 81.7 NP_002502 390 162341 47.8 96.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0257 Download

>KIEE0257  1197 bp
ATGGCTCAAAGGCAGCCTCACTCACCTAATCAGACTTTAATTTCAATCACAAATGACACA
GAATCATCAAGCTCTGTGGTTTCTAACGATAACACAAATAAAGGATGGAGCGGGGACAAC
TCTCCAGGAATAGAAGCATTGTGTGCCATCTATATTACTTATGCTGTGATCATTTCAGTG
GGCATCCTTGGAAATGCTATTCTCATCAAAGTCTTTTTCAAGACCAAATCCATGCAAACA
GTTCCAAATATTTTCATCACCAGCCTGGCTTTTGGAGATCTTTTACTTCTGCTAACTTGT
GTGCCAGTGGATGCAACTCACTACCTTGCAGAAGGATGGCTGTTCGGAAGAATTGGTTGT
AAGGTGCTCTCTTTCATCCGGCTCACTTCTGTTGGTGTGTCAGTGTTCACATTAACAATT
CTCAGCGCTGACAGATACAAGGCAGTTGTGAAGCCACTTGAGCGACAGCCCTCCAATGCC
ATCCTGAAGACTTGTGTAAAAGCTGGCTGCGTCTGGATCGTGTCTATGATATTTGCTCTA
CCTGAGGCTATATTTTCAAATGTATACACTTTTCGAGATCCCAATAAAAATATGACATTT
GAATCATGTACCTCTTATCCTGTCTCTAAGAAGCTCTTGCAAGAAATACATTCTCTGCTG
TGCTTCTTAGTGTTCTACATTATTCCACTCTCTATTATCTCTGTCTACTATTCCTTGATT
GCTAGGACCCTTTACAAAAGCACCCTGAACATACCTACTGAGGAACAAAGCCATGCCCGT
AAGCAGATTGAATCCCGAAAGAGAATTGCCAGAACGGTATTGGTGTTGGTGGCTCTGTTT
GCCCTCTGCTGGTTGCCAAATCACCTCCTGTACCTCTACCATTCATTCACTTCTCAAACC
TATGTAGACCCCTCTGCCATGCATTTCATTTTCACCATTTTCTCTCGGGTTTTGGCTTTC
AGCAATTCTTGCGTAAACCCCTTTGCTCTCTACTGGCTGAGCAAAAGCTTCCAGAAGCAT
TTTAAAGCTCAGTTGTTCTGTTGCAAGGCGGAGCGGCCTGAGCCTCCTGTTGCTGACACC
TCTCTTACCACCCTGGCTGTGATGGGAACGGTCCCGGGCACTGGGAGCATACAGATGTCT
GAAATTAGTGTGACCTCGTTCACTGGGTGTAGTGTGAAGCAGGCAGAGGACAGATTC

ORF Protein

Cloned ORF protein sequence for pFN21AE0257 Download

>KIEE0257  399 aa
MAQRQPHSPNQTLISITNDTESSSSVVSNDNTNKGWSGDNSPGIEALCAIYITYAVIISV
GILGNAILIKVFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVDATHYLAEGWLFGRIGC
KVLSFIRLTSVGVSVFTLTILSADRYKAVVKPLERQPSNAILKTCVKAGCVWIVSMIFAL
PEAIFSNVYTFRDPNKNMTFESCTSYPVSKKLLQEIHSLLCFLVFYIIPLSIISVYYSLI
ARTLYKSTLNIPTEEQSHARKQIESRKRIARTVLVLVALFALCWLPNHLLYLYHSFTSQT
YVDPSAMHFIFTIFSRVLAFSNSCVNPFALYWLSKSFQKHFKAQLFCCKAERPEPPVADT
SLTTLAVMGTVPGTGSIQMSEISVTSFTGCSVKQAEDRF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0257 Download

>pFN21AE0257  5902 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTCAAAGGCAGCCTCACTCACCTAATCAGAC
TTTAATTTCAATCACAAATGACACAGAATCATCAAGCTCTGTGGTTTCTAACGATAACAC
AAATAAAGGATGGAGCGGGGACAACTCTCCAGGAATAGAAGCATTGTGTGCCATCTATAT
TACTTATGCTGTGATCATTTCAGTGGGCATCCTTGGAAATGCTATTCTCATCAAAGTCTT
TTTCAAGACCAAATCCATGCAAACAGTTCCAAATATTTTCATCACCAGCCTGGCTTTTGG
AGATCTTTTACTTCTGCTAACTTGTGTGCCAGTGGATGCAACTCACTACCTTGCAGAAGG
ATGGCTGTTCGGAAGAATTGGTTGTAAGGTGCTCTCTTTCATCCGGCTCACTTCTGTTGG
TGTGTCAGTGTTCACATTAACAATTCTCAGCGCTGACAGATACAAGGCAGTTGTGAAGCC
ACTTGAGCGACAGCCCTCCAATGCCATCCTGAAGACTTGTGTAAAAGCTGGCTGCGTCTG
GATCGTGTCTATGATATTTGCTCTACCTGAGGCTATATTTTCAAATGTATACACTTTTCG
AGATCCCAATAAAAATATGACATTTGAATCATGTACCTCTTATCCTGTCTCTAAGAAGCT
CTTGCAAGAAATACATTCTCTGCTGTGCTTCTTAGTGTTCTACATTATTCCACTCTCTAT
TATCTCTGTCTACTATTCCTTGATTGCTAGGACCCTTTACAAAAGCACCCTGAACATACC
TACTGAGGAACAAAGCCATGCCCGTAAGCAGATTGAATCCCGAAAGAGAATTGCCAGAAC
GGTATTGGTGTTGGTGGCTCTGTTTGCCCTCTGCTGGTTGCCAAATCACCTCCTGTACCT
CTACCATTCATTCACTTCTCAAACCTATGTAGACCCCTCTGCCATGCATTTCATTTTCAC
CATTTTCTCTCGGGTTTTGGCTTTCAGCAATTCTTGCGTAAACCCCTTTGCTCTCTACTG
GCTGAGCAAAAGCTTCCAGAAGCATTTTAAAGCTCAGTTGTTCTGTTGCAAGGCGGAGCG
GCCTGAGCCTCCTGTTGCTGACACCTCTCTTACCACCCTGGCTGTGATGGGAACGGTCCC
GGGCACTGGGAGCATACAGATGTCTGAAATTAGTGTGACCTCGTTCACTGGGTGTAGTGT
GAAGCAGGCAGAGGACAGATTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT