(Circular) (Six-base) MAPSORT of: pFN21AE0257.seq Check: 5050 from: 1 to: 5902 pFN21AE0257 5902 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2568 2709 2910 3245 1937 Size: 631 141 201 335 4594 Fragments arranged by size: 4594 631 335 201 141 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 3965 5349 5617 1632 Size: 878 1455 1384 268 1917 Fragments arranged by size: 1917 1455 1384 878 268 AfeI AGC'GCT Cuts at: 2431 2431 Size: 5902 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 3645 5217 3645 Size: 1572 4330 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 3211 3437 3589 5698 1493 Size: 1718 226 152 2109 1697 Fragments arranged by size: 2109 1718 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2949 3118 3227 1870 Size: 73 1006 169 109 4545 Fragments arranged by size: 4545 1006 169 109 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3223 4678 618 Size: 325 217 627 1436 1455 1842 Fragments arranged by size: 1842 1455 1436 627 325 217 BanII G_rGCy'C Cuts at: 1391 3221 1391 Size: 1830 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2498 928 Size: 971 599 4332 Fragments arranged by size: 4332 971 599 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 2172 1548 Size: 624 5278 BfrBI ATG'CAT Cuts at: 2926 2926 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2281 5897 2281 Size: 3616 2286 BlpI GC'TnA_GC Cuts at: 3002 3319 3002 Size: 317 5585 Bme1580I G_kGCm'C Cuts at: 1790 3125 3963 5119 1790 Size: 1335 838 1156 2573 Fragments arranged by size: 2573 1335 1156 838 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3135 3177 3633 Size: 416 53 83 399 24 1718 42 456 Cuts at: 3633 4591 442 Size: 958 1753 Fragments arranged by size: 1753 1718 958 456 416 399 83 53 42 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2112 4562 1239 Size: 873 2450 2579 Fragments arranged by size: 2579 2450 873 Bpu10I CC'TnA_GC Cuts at: 3063 3063 Size: 5902 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2483 4027 4892 5190 2483 Size: 1544 865 298 3195 Fragments arranged by size: 3195 1544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3693 493 Size: 711 2489 2702 Fragments arranged by size: 2702 2489 711 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3709 4091 4726 275 Size: 53 83 186 936 2176 382 635 1451 Fragments arranged by size: 2176 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4335 4733 5007 5154 5565 4335 Size: 398 274 147 411 4672 Fragments arranged by size: 4672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5677 Fragments arranged by size: 5677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5902 BseYI C'CCAG_C Cuts at: 5105 5601 5105 Size: 496 5406 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3352 4113 5141 1540 Size: 275 186 1351 761 1028 2301 Fragments arranged by size: 2301 1351 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2373 3963 4048 5119 2373 Size: 1590 85 1071 3156 Fragments arranged by size: 3156 1590 1071 85 BsmI GAATG_Cn' Cuts at: 3413 3506 3413 Size: 93 5809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2373 3125 3221 3963 4048 5119 1391 Size: 399 583 752 96 742 85 1071 2174 Fragments arranged by size: 2174 1071 752 742 583 399 96 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3257 844 Size: 2413 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2115 3058 3789 1394 Size: 429 292 943 731 3507 Fragments arranged by size: 3507 943 731 429 292 BsrDI GCAATG_nn' Cuts at: 65 1248 4397 4571 65 Size: 1183 3149 174 1396 Fragments arranged by size: 3149 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4546 4733 1700 Size: 110 2736 187 2869 Fragments arranged by size: 2869 2736 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 2147 2973 794 Size: 1353 826 3723 Fragments arranged by size: 3723 1353 826 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2281 2581 3232 3599 3984 4001 5453 5539 Size: 309 300 651 367 385 17 1452 86 Cuts at: 5539 5551 5897 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 651 385 367 346 309 300 86 17 12 BstZ17I GTA'TAC Cuts at: 2569 2569 Size: 5902 Bsu36I CC'TnA_GG Cuts at: 2547 2547 Size: 5902 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4411 Fragments arranged by size: 4411 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 3418 4212 4232 3418 Size: 794 20 5088 Fragments arranged by size: 5088 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2061 2096 529 Size: 35 1497 35 4335 Fragments arranged by size: 4335 1497 35 35 DraI TTT'AAA Cuts at: 3028 3206 3028 Size: 178 5724 DraIII CAC_nnn'GTG Cuts at: 3171 3171 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867 Size: 54 1425 50 275 18 1519 889 1629 Cuts at: 5867 8 Size: 43 Fragments arranged by size: 1629 1519 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3832 1353 Size: 2479 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3068 4489 4863 5009 387 Size: 1064 138 1479 1421 374 146 1280 Fragments arranged by size: 1479 1421 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2510 3965 4562 5349 5617 1239 Size: 393 480 398 1455 597 787 268 1524 Fragments arranged by size: 1524 1455 787 597 480 398 393 268 EcoO109I rG'GnC_Cy Cuts at: 2730 2730 Size: 5902 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2656 2656 Size: 5902 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 2433 5049 1678 Size: 755 2616 2531 Fragments arranged by size: 2616 2531 755 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2524 2556 4543 Size: 32 135 32 479 32 441 32 1987 Cuts at: 4543 4575 4617 4649 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1987 479 441 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3246 3492 669 Size: 988 281 1308 246 3079 Fragments arranged by size: 3079 1308 988 281 246 HindIII A'AGCT_T Cuts at: 748 3010 748 Size: 2262 3640 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5016 5200 1305 Size: 3711 184 2007 Fragments arranged by size: 3711 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4080 Fragments arranged by size: 4080 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 3137 3860 4219 4378 518 Size: 648 1014 957 723 359 159 2042 Fragments arranged by size: 2042 1014 957 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3318 3995 5143 5388 1606 Size: 1712 677 1148 245 2120 Fragments arranged by size: 2120 1712 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4411 Fragments arranged by size: 4411 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NsiI A_TGCA'T Cuts at: 2928 2928 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 3260 4805 1132 Size: 97 2031 1545 2229 Fragments arranged by size: 2229 2031 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5902 PfoI T'CCnGG_A Cuts at: 2128 2128 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2730 2730 Size: 5902 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PstI C_TGCA'G Cuts at: 830 3254 830 Size: 2424 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 3250 4385 5066 5257 826 Size: 219 2205 1135 681 191 1471 Fragments arranged by size: 2205 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3120 3229 1872 Size: 1248 109 4545 Fragments arranged by size: 4545 1248 109 SmlI C'TyrA_G Cuts at: 820 1017 1943 2462 4006 4907 5169 820 Size: 197 926 519 1544 901 262 1553 Fragments arranged by size: 1553 1544 926 901 519 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 2254 3826 5 Size: 47 2202 1572 2081 Fragments arranged by size: 2202 2081 1572 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5902 StyI C'CwwG_G Cuts at: 513 1065 2004 2191 3341 513 Size: 552 939 187 1150 3074 Fragments arranged by size: 3074 1150 939 552 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3159 3929 3946 4099 4284 4729 325 Size: 1255 1579 770 17 153 185 445 1498 Fragments arranged by size: 1579 1498 1255 770 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2821 2821 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3118 3227 1870 Size: 1248 109 4545 Fragments arranged by size: 4545 1248 109 XmnI GAAnn'nnTTC Cuts at: 4031 4031 Size: 5902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI