BTBD2 ORF clone pFN21AE0086

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Halotag ORF Clone pFN21AE0086

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Clone info.

Species

human

Clone Name

pFN21AE0086 Sequence (with vector) Restriction Map

Product ID

FHC10757

Flexi Clone PID

FXC10757

ORF Sequence

DNA sequence (1575 bp)
Protein sequence (525 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527352]

Gene

KIEE0086

Symbol

BTBD2

Description

BTB (POZ) domain containing 2

Original Clone ID

oc00786

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1575 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 525 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12078.1 3.3e-100 100.0 BTBD2 CCDS10322.1 3.3e-70 77.6 BTBD1 CCDS32313.1 2.6e-49 77.6 BTBD1 CCDS10002.2 7.5e-36 45.1 BTBD6 CCDS13114.1 3.1e-34 46.6 BTBD3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 110 216 PF00651 BTB/POZ IPR011705 225 326 PF07707 BTB/Kelch-associated IPR012983 375 525 PF08005 PHR HMMSmart IPR000210 117 217 SM00225 BTB/POZ-like IPR011705 222 330 SM00875 BTB/Kelch-associated ProfileScan IPR000210 117 187 PS50097 BTB/POZ-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060267 525 608531 100.0 100.0 NP_079514 482 608530 77.6 92.2 XP_016883217 461 615566 46.6 82.3 XP_016883218 461 615566 46.6 82.3 NP_001269479 461 615566 46.6 82.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0086 Download

>KIEE0086  1575 bp
ATGGCGGCGGGTGGGAGCGGCGGGCGTGCGTCGTGCCCGCCGGGGGTCGGGGTCGGCCCG
GGCACGGGGGGCAGTCCCGGGCCCAGCGCCAACGCCGCCGCCACCCCGGCCCCCGGCAAC
GCGGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCCCTGGGCCGACGCCGCCC
GCCCCGCCGGGCCCCGGGACAGACGCGCAGGCCGCGGGCGCGGAGCGGGCGGAGGAGGCG
GCGGGCCCGGGGGCGGCGGCGCTGCAGCGCGAGGCCGCGTACAACTGGCAGGCCAGCAAG
CCCACCGTGCAGGAGCGCTTCGCCTTCCTCTTCAACAACGAGGTGCTGTGCGACGTGCAC
TTCCTGGTGGGCAAGGGGCTCAGCTCGCAGCGCATCCCCGCGCACAGGTTCGTGCTGGCC
GTGGGCAGCGCCGTCTTTGATGCCATGTTCAACGGGGGAATGGCCACAACATCCACGGAG
ATTGAGCTGCCCGACGTGGAACCCGCTGCCTTCCTCGCACTGCTCAAGTTTCTCTACTCG
GACGAGGTGCAGATTGGCCCGGAGACGGTGATGACCACGCTATACACCGCCAAGAAGTAC
GCGGTGCCAGCGCTCGAGGCCCATTGCGTGGAGTTCCTGAAGAAGAACCTGCGAGCCGAC
AACGCCTTCATGCTGCTCACGCAGGCGCGACTCTTCGATGAACCGCAGCTGGCCAGCCTG
TGCCTGGAGAACATCGACAAAAACACTGCAGACGCCATCACCGCGGAGGGCTTCACCGAC
ATTGACCTGGACACGCTGGTGGCTGTCCTGGAGCGCGACACACTGGGCATCCGTGAGGTG
CGGCTGTTCAATGCCGTTGTCCGCTGGTCCGAGGCCGAGTGTCAGCGGCAGCAGCTGCAG
GTGACGCCAGAGAACAGGCGGAAGGTTCTGGGCAAGGCCCTGGGCCTCATTCGCTTCCCG
CTCATGACCATCGAGGAGTTCGCTGCAGGTCCCGCACAGTCGGGCATCCTGGTGGACCGC
GAGGTGGTCAGCCTCTTCCTGCACTTCACCGTCAACCCCAAGCCACGAGTGGAGTTCATT
GACCGGCCCCGCTGCTGCCTGCGTGGGAAGGAGTGCAGCATCAACCGCTTCCAGCAGGTG
GAGAGTCGCTGGGGCTACAGCGGGACCAGTGACCGCATCAGGTTCTCAGTCAACAAGCGC
ATCTTCGTGGTGGGATTTGGGCTGTATGGATCCATCCACGGGCCCACCGACTACCAAGTG
AACATCCAGATTATTCACACCGATAGCAACACCGTCTTGGGCCAGAACGACACGGGCTTC
AGCTGCGACGGCTCAGCCAGCACCTTCCGCGTCATGTTCAAGGAGCCGGTGGAGGTGCTG
CCCAACGTCAACTACACGGCCTGTGCCACGCTCAAGGGCCCAGACTCCCACTACGGCACC
AAAGGCCTGCGCAAGGTGACACACGAGTCGCCCACCACGGGCGCCAAGACCTGCTTCACC
TTTTGCTACGCGGCCGGGAACAACAATGGCACATCCGTGGAGGACGGCCAGATCCCCGAG
GTCATCTTCTACACC

ORF Protein

Cloned ORF protein sequence for pFN21AE0086 Download

>KIEE0086  525 aa
MAAGGSGGRASCPPGVGVGPGTGGSPGPSANAAATPAPGNAAAAAAAAAAAAAAPGPTPP
APPGPGTDAQAAGAERAEEAAGPGAAALQREAAYNWQASKPTVQERFAFLFNNEVLCDVH
FLVGKGLSSQRIPAHRFVLAVGSAVFDAMFNGGMATTSTEIELPDVEPAAFLALLKFLYS
DEVQIGPETVMTTLYTAKKYAVPALEAHCVEFLKKNLRADNAFMLLTQARLFDEPQLASL
CLENIDKNTADAITAEGFTDIDLDTLVAVLERDTLGIREVRLFNAVVRWSEAECQRQQLQ
VTPENRRKVLGKALGLIRFPLMTIEEFAAGPAQSGILVDREVVSLFLHFTVNPKPRVEFI
DRPRCCLRGKECSINRFQQVESRWGYSGTSDRIRFSVNKRIFVVGFGLYGSIHGPTDYQV
NIQIIHTDSNTVLGQNDTGFSCDGSASTFRVMFKEPVEVLPNVNYTACATLKGPDSHYGT
KGLRKVTHESPTTGAKTCFTFCYAAGNNNGTSVEDGQIPEVIFYT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0086 Download

>pFN21AE0086  6280 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCGGGTGGGAGCGGCGGGCGTGCGTCGTG
CCCGCCGGGGGTCGGGGTCGGCCCGGGCACGGGGGGCAGTCCCGGGCCCAGCGCCAACGC
CGCCGCCACCCCGGCCCCCGGCAACGCGGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGC
CGCCGCCCCTGGGCCGACGCCGCCCGCCCCGCCGGGCCCCGGGACAGACGCGCAGGCCGC
GGGCGCGGAGCGGGCGGAGGAGGCGGCGGGCCCGGGGGCGGCGGCGCTGCAGCGCGAGGC
CGCGTACAACTGGCAGGCCAGCAAGCCCACCGTGCAGGAGCGCTTCGCCTTCCTCTTCAA
CAACGAGGTGCTGTGCGACGTGCACTTCCTGGTGGGCAAGGGGCTCAGCTCGCAGCGCAT
CCCCGCGCACAGGTTCGTGCTGGCCGTGGGCAGCGCCGTCTTTGATGCCATGTTCAACGG
GGGAATGGCCACAACATCCACGGAGATTGAGCTGCCCGACGTGGAACCCGCTGCCTTCCT
CGCACTGCTCAAGTTTCTCTACTCGGACGAGGTGCAGATTGGCCCGGAGACGGTGATGAC
CACGCTATACACCGCCAAGAAGTACGCGGTGCCAGCGCTCGAGGCCCATTGCGTGGAGTT
CCTGAAGAAGAACCTGCGAGCCGACAACGCCTTCATGCTGCTCACGCAGGCGCGACTCTT
CGATGAACCGCAGCTGGCCAGCCTGTGCCTGGAGAACATCGACAAAAACACTGCAGACGC
CATCACCGCGGAGGGCTTCACCGACATTGACCTGGACACGCTGGTGGCTGTCCTGGAGCG
CGACACACTGGGCATCCGTGAGGTGCGGCTGTTCAATGCCGTTGTCCGCTGGTCCGAGGC
CGAGTGTCAGCGGCAGCAGCTGCAGGTGACGCCAGAGAACAGGCGGAAGGTTCTGGGCAA
GGCCCTGGGCCTCATTCGCTTCCCGCTCATGACCATCGAGGAGTTCGCTGCAGGTCCCGC
ACAGTCGGGCATCCTGGTGGACCGCGAGGTGGTCAGCCTCTTCCTGCACTTCACCGTCAA
CCCCAAGCCACGAGTGGAGTTCATTGACCGGCCCCGCTGCTGCCTGCGTGGGAAGGAGTG
CAGCATCAACCGCTTCCAGCAGGTGGAGAGTCGCTGGGGCTACAGCGGGACCAGTGACCG
CATCAGGTTCTCAGTCAACAAGCGCATCTTCGTGGTGGGATTTGGGCTGTATGGATCCAT
CCACGGGCCCACCGACTACCAAGTGAACATCCAGATTATTCACACCGATAGCAACACCGT
CTTGGGCCAGAACGACACGGGCTTCAGCTGCGACGGCTCAGCCAGCACCTTCCGCGTCAT
GTTCAAGGAGCCGGTGGAGGTGCTGCCCAACGTCAACTACACGGCCTGTGCCACGCTCAA
GGGCCCAGACTCCCACTACGGCACCAAAGGCCTGCGCAAGGTGACACACGAGTCGCCCAC
CACGGGCGCCAAGACCTGCTTCACCTTTTGCTACGCGGCCGGGAACAACAATGGCACATC
CGTGGAGGACGGCCAGATCCCCGAGGTCATCTTCTACACCGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT