(Circular) (Six-base) MAPSORT of: pFN21AE0086.seq Check: 4598 from: 1 to: 6280 pFN21AE0086 6280 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2893 3130 2893 Size: 237 6043 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4090 278 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3623 1937 Size: 1686 4594 Acc65I G'GTAC_C Cuts at: 3601 3601 Size: 6280 AclI AA'CG_TT Cuts at: 1563 4407 4780 1563 Size: 2844 373 3063 Fragments arranged by size: 3063 2844 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2663 3307 4343 5727 5995 1632 Size: 1031 644 1036 1384 268 1917 Fragments arranged by size: 1917 1384 1036 1031 644 268 AfeI AGC'GCT Cuts at: 2321 2616 2321 Size: 295 5985 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6083 AflIII A'CryG_T Cuts at: 1203 5179 6156 1203 Size: 3976 977 1327 Fragments arranged by size: 3976 1327 977 AgeI A'CCGG_T Cuts at: 5111 5111 Size: 6280 AhdI GACnn_n'nnGTC Cuts at: 5009 5009 Size: 6280 AleI CACnn'nnGTG Cuts at: 1166 2801 3539 1166 Size: 1635 738 3907 Fragments arranged by size: 3907 1635 738 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6280 AlwNI CAG_nnn'CTG Cuts at: 2899 4023 5595 2899 Size: 1124 1572 3584 Fragments arranged by size: 3584 1572 1124 ApaI G_GGCC'C Cuts at: 2088 2198 2252 3249 3425 2088 Size: 110 54 997 176 4943 Fragments arranged by size: 4943 997 176 110 54 ApaLI G'TGCA_C Cuts at: 2360 4337 5493 2360 Size: 1977 1156 3147 Fragments arranged by size: 3147 1977 1156 ApoI r'AATT_y Cuts at: 1493 3589 3815 3967 6076 1493 Size: 2096 226 152 2109 1697 Fragments arranged by size: 2109 2096 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4834 160 Size: 872 3802 1606 Fragments arranged by size: 3802 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2081 2198 2251 2618 3560 3605 Size: 73 119 19 117 53 367 942 45 Cuts at: 3605 1870 Size: 4545 Fragments arranged by size: 4545 942 367 119 117 73 53 45 19 BamHI G'GATC_C Cuts at: 3233 3610 3977 3233 Size: 377 367 5536 Fragments arranged by size: 5536 377 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2608 3440 3485 3601 5056 Size: 325 217 627 821 832 45 116 1455 Cuts at: 5056 618 Size: 1842 Fragments arranged by size: 1842 1455 832 821 627 325 217 116 45 BanII G_rGCy'C Cuts at: 1391 2088 2198 2252 2385 3249 3425 3599 1391 Size: 697 110 54 133 864 176 174 4072 Fragments arranged by size: 4072 864 697 176 174 133 110 54 BbeI G_GCGC'C Cuts at: 3489 3489 Size: 6280 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5309 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4471 4505 1903 Size: 34 2534 34 3678 Fragments arranged by size: 3678 2534 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4172 5382 4172 Size: 1210 5070 BclI T'GATC_A Cuts at: 1548 1548 Size: 6280 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2111 4891 Size: 107 122 71 631 605 337 102 2780 Cuts at: 4891 136 Size: 1525 Fragments arranged by size: 2780 1525 631 605 337 122 107 102 71 BglII A'GATC_T Cuts at: 6275 6275 Size: 6280 BlpI GC'TnA_GC Cuts at: 2384 3337 3697 2384 Size: 953 360 4967 Fragments arranged by size: 4967 953 360 Bme1580I G_kGCm'C Cuts at: 1790 2042 2069 2088 2198 2252 2364 3249 3425 Size: 252 27 19 110 54 112 885 176 Cuts at: 3425 4341 5497 1790 Size: 916 1156 2573 Fragments arranged by size: 2573 1156 916 885 252 176 112 110 54 27 19 BmgBI CAC'GTC Cuts at: 1318 2359 2500 1318 Size: 1041 141 5098 Fragments arranged by size: 5098 1041 141 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2837 4011 4969 Size: 416 53 83 399 24 1420 1174 958 Cuts at: 4969 442 Size: 1753 Fragments arranged by size: 1753 1420 1174 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2750 2834 4940 1239 Size: 1511 84 2106 2579 Fragments arranged by size: 2579 2106 1511 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 3401 4405 5270 5568 2513 Size: 888 1004 865 298 3225 Fragments arranged by size: 3225 1004 888 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4943 882 Size: 617 3444 2219 Fragments arranged by size: 3444 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4071 493 Size: 711 2867 2702 Fragments arranged by size: 2867 2702 711 BsaBI GATnn'nnATC Cuts at: 6274 6274 Size: 6280 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2177 2757 2909 3486 Size: 53 83 186 936 644 580 152 577 Cuts at: 3486 4087 4469 5104 275 Size: 601 382 635 1451 Fragments arranged by size: 1451 936 644 635 601 580 577 382 186 152 83 53 BsaWI w'CCGG_w Cuts at: 4713 5111 5385 5532 5943 4713 Size: 398 274 147 411 5050 Fragments arranged by size: 5050 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6055 Fragments arranged by size: 6055 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 2993 1186 Size: 1066 741 4473 Fragments arranged by size: 4473 1066 741 BseYI C'CCAG_C Cuts at: 2087 3153 5483 5979 2087 Size: 1066 2330 496 2388 Fragments arranged by size: 2388 2330 1066 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 2573 3029 3139 1163 Size: 573 597 240 456 110 4304 Fragments arranged by size: 4304 597 573 456 240 110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2130 3519 3730 4491 5519 1540 Size: 275 186 129 1389 211 761 1028 2301 Fragments arranged by size: 2301 1389 1028 761 275 211 186 129 BsiHKAI G_wGCw'C Cuts at: 2364 4341 4426 5497 2364 Size: 1977 85 1071 3147 Fragments arranged by size: 3147 1977 1071 85 BsmI GAATG_Cn' Cuts at: 3791 3884 3791 Size: 93 6187 BsmBI CGTCTCn'nnnn_ Cuts at: 2561 2561 Size: 6280 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2069 2088 2198 2252 2364 2385 Size: 399 252 27 19 110 54 112 21 Cuts at: 2385 3249 3425 3599 4341 4426 5497 1391 Size: 864 176 174 742 85 1071 2174 Fragments arranged by size: 2174 1071 864 742 399 252 176 174 112 110 85 54 27 21 19 BspEI T'CCGG_A Cuts at: 5943 5943 Size: 6280 BspHI T'CATG_A Cuts at: 2967 4169 5899 2967 Size: 1202 1730 3348 Fragments arranged by size: 3348 1730 1202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2661 2893 2981 3130 3503 3635 844 Size: 1817 232 88 149 373 132 3489 Fragments arranged by size: 3489 1817 373 232 149 132 88 BsrBI CCG'CTC Cuts at: 1394 1823 2022 2230 2965 4167 1394 Size: 429 199 208 735 1202 3507 Fragments arranged by size: 3507 1202 735 429 208 199 BsrDI GCAATG_nn' Cuts at: 65 1248 2626 4775 4949 65 Size: 1183 1378 2149 174 1396 Fragments arranged by size: 2149 1396 1378 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3087 3370 4924 5111 1700 Size: 110 1277 283 1554 187 2869 Fragments arranged by size: 2869 1554 1277 283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6280 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6280 BssSI C'ACGA_G Cuts at: 3069 3467 4340 5352 3069 Size: 398 873 1012 3997 Fragments arranged by size: 3997 1012 873 398 BstAPI GCAn_nnn'nTGC Cuts at: 794 2899 794 Size: 2105 4175 BstBI TT'CG_AA Cuts at: 5084 5084 Size: 6280 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6280 BstYI r'GATC_y Cuts at: 1972 3233 3555 3610 3977 4362 4379 5831 5917 Size: 1261 322 55 367 385 17 1452 86 Cuts at: 5917 5929 6275 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1261 385 367 346 322 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2217 2424 2478 2766 3241 3480 Size: 552 939 213 207 54 288 475 239 Cuts at: 3480 3540 513 Size: 60 3253 Fragments arranged by size: 3253 939 552 475 288 239 213 207 60 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5541 Fragments arranged by size: 5541 683 56 BtsI GCAGTG_nn' Cuts at: 2522 2748 3796 4590 4610 2522 Size: 226 1048 794 20 4192 Fragments arranged by size: 4192 1048 794 226 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6280 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2467 2502 529 Size: 35 1903 35 4307 Fragments arranged by size: 4307 1903 35 35 DraI TTT'AAA Cuts at: 3584 3584 Size: 6280 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3469 5287 809 Size: 507 2153 1818 1802 Fragments arranged by size: 2153 1818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2421 2466 Size: 54 1425 50 275 18 297 294 45 Cuts at: 2466 2715 3516 3550 3727 4616 6245 8 Size: 249 801 34 177 889 1629 43 Fragments arranged by size: 1629 1425 889 801 297 294 275 249 177 54 50 45 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 2127 3516 3727 1537 Size: 275 315 1389 211 4090 Fragments arranged by size: 4090 1389 315 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 2339 2702 3044 4210 1353 Size: 986 363 342 1166 3423 Fragments arranged by size: 3423 1166 986 363 342 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 2938 4867 5241 5387 387 Size: 1064 138 660 689 1929 374 146 1280 Fragments arranged by size: 1929 1280 1064 689 660 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2663 2750 2834 3307 4343 4940 5727 Size: 393 1031 87 84 473 1036 597 787 Cuts at: 5727 5995 1239 Size: 268 1524 Fragments arranged by size: 1524 1036 1031 787 597 473 393 268 87 84 EcoNI CCTnn'n_nnAGG Cuts at: 3455 3455 Size: 6280 EcoO109I rG'GnC_Cy Cuts at: 2195 2941 2993 3421 2195 Size: 746 52 428 5054 Fragments arranged by size: 5054 746 428 52 EcoRI G'AATT_C Cuts at: 3589 3967 3589 Size: 378 5902 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3223 3514 3223 Size: 291 5989 FspI TGC'GCA Cuts at: 3455 4786 3455 Size: 1331 4949 HaeII r_GCGC'y Cuts at: 1678 2094 2267 2323 2436 2618 3489 5427 1678 Size: 416 173 56 113 182 871 1938 2531 Fragments arranged by size: 2531 1938 871 416 182 173 113 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4921 4953 4995 5027 1373 Size: 32 135 32 3349 32 42 32 2626 Fragments arranged by size: 3349 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3058 3196 3394 3624 3870 669 Size: 988 281 1120 138 198 230 246 3079 Fragments arranged by size: 3079 1120 988 281 246 230 198 138 HindIII A'AGCT_T Cuts at: 748 748 Size: 6280 HpaI GTT'AAC Cuts at: 3870 3870 Size: 6280 KasI G'GCGC_C Cuts at: 3485 3485 Size: 6280 KpnI G_GTAC'C Cuts at: 3605 3605 Size: 6280 MfeI C'AATT_G Cuts at: 3879 3879 Size: 6280 MluI A'CGCG_T Cuts at: 6156 6156 Size: 6280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5394 5578 1305 Size: 4089 184 2007 Fragments arranged by size: 4089 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2468 2717 10 Size: 54 1425 343 636 249 3573 Fragments arranged by size: 3573 1425 636 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2801 3014 3209 3539 4238 4597 4756 Size: 648 1635 213 195 330 699 359 159 Cuts at: 4756 518 Size: 2042 Fragments arranged by size: 2042 1635 699 648 359 330 213 195 159 MspA1I CmG'CkG Cuts at: 1606 2219 2510 2713 2768 2868 2890 2899 3096 Size: 613 291 203 55 100 22 9 197 Cuts at: 3096 3165 3327 3696 4373 5521 5766 1606 Size: 69 162 369 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 613 369 291 245 203 197 162 100 69 55 22 9 NarI GG'CG_CC Cuts at: 3486 3486 Size: 6280 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4789 Fragments arranged by size: 4789 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6280 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6280 NotI GC'GGCC_GC Cuts at: 2127 3727 2127 Size: 1600 4680 NspI r_CATG'y Cuts at: 1132 1229 3638 5183 1132 Size: 97 2409 1545 2229 Fragments arranged by size: 2409 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2168 2944 1348 Size: 820 776 4684 Fragments arranged by size: 4684 820 776 PciI A'CATG_T Cuts at: 5179 5179 Size: 6280 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 3142 1108 Size: 1524 510 4246 Fragments arranged by size: 4246 1524 510 PfoI T'CCnGG_A Cuts at: 2811 2811 Size: 6280 PmeI GTTT'AAAC Cuts at: 3584 3584 Size: 6280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4349 4381 5862 5894 4349 Size: 32 1481 32 4735 Fragments arranged by size: 4735 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2993 2993 Size: 6280 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6280 PsiI TTA'TAA Cuts at: 3850 3850 Size: 6280 PspOMI G'GGCC_C Cuts at: 2084 2194 2248 3245 3421 2084 Size: 110 54 997 176 4943 Fragments arranged by size: 4943 997 176 110 54 PspXI vC'TCGA_Gb Cuts at: 2618 2618 Size: 6280 PstI C_TGCA'G Cuts at: 830 2271 2755 2904 2992 3632 830 Size: 1441 484 149 88 640 3478 Fragments arranged by size: 3478 1441 640 484 149 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6280 PvuII CAG'CTG Cuts at: 2713 2899 3327 2713 Size: 186 428 5666 Fragments arranged by size: 5666 428 186 SacII CC_GC'GG Cuts at: 2220 2769 2220 Size: 549 5731 SalI G'TCGA_C Cuts at: 1936 3622 1936 Size: 1686 4594 SbfI CC_TGCA'GG Cuts at: 3632 3632 Size: 6280 ScaI AGT'ACT Cuts at: 4528 6240 4528 Size: 1712 4568 SfcI C'TryA_G Cuts at: 826 1045 2267 2751 2900 2988 3160 3628 4763 Size: 219 1222 484 149 88 172 468 1135 Cuts at: 4763 5444 5635 826 Size: 681 191 1471 Fragments arranged by size: 1471 1222 1135 681 484 468 219 191 172 149 88 SfoI GGC'GCC Cuts at: 3487 3487 Size: 6280 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6280 SmaI CCC'GGG Cuts at: 1872 2064 2083 2200 2253 3607 1872 Size: 192 19 117 53 1354 4545 Fragments arranged by size: 4545 1354 192 117 53 19 SmlI C'TyrA_G Cuts at: 820 1017 1943 2528 2618 3416 4384 5285 5547 Size: 197 926 585 90 798 968 901 262 Cuts at: 5547 820 Size: 1553 Fragments arranged by size: 1553 968 926 901 798 585 262 197 90 SnaBI TAC'GTA Cuts at: 493 4071 493 Size: 3578 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6280 SphI G_CATG'C Cuts at: 1132 3638 1132 Size: 2506 3774 SrfI GCCC'GGGC Cuts at: 2064 2064 Size: 6280 SspI AAT'ATT Cuts at: 5 52 4204 5 Size: 47 4152 2081 Fragments arranged by size: 4152 2081 47 StuI AGG'CCT Cuts at: 1517 3450 1517 Size: 1933 4347 StyI C'CwwG_G Cuts at: 513 1065 2004 3719 513 Size: 552 939 1715 3074 Fragments arranged by size: 3074 1715 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4307 4324 4477 4662 5107 325 Size: 1255 2727 17 153 185 445 1498 Fragments arranged by size: 2727 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4526 6238 96 Size: 274 80 33 51 1443 2549 1712 138 Fragments arranged by size: 2549 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2817 2849 6240 6272 2817 Size: 32 3391 32 2825 Fragments arranged by size: 3391 2825 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6280 XbaI T'CTAG_A Cuts at: 3616 3616 Size: 6280 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2725 3070 2725 Size: 345 5935 XhoI C'TCGA_G Cuts at: 1943 2618 1943 Size: 675 5605 XmaI C'CCGG_G Cuts at: 1870 2062 2081 2198 2251 3605 1870 Size: 192 19 117 53 1354 4545 Fragments arranged by size: 4545 1354 192 117 53 19 XmnI GAAnn'nnTTC Cuts at: 4409 4409 Size: 6280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4088 276 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI