FIGN ORF clone pFN21AE0058

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0058

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Clone info.

Species

human

Clone Name

pFN21AE0058 Sequence (with vector) Restriction Map

Product ID

FHC10733

Flexi Clone PID

FXC10733

ORF Sequence

DNA sequence (2277 bp)
Protein sequence (759 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527328]

Gene

KIEE0058

Symbol

FIGN

Description

fidgetin

Original Clone ID

oc00758

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2277 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 759 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2221.2 5.9e-111 100.0 FIGN CCDS5510.1 9.4e-25 38.0 FIGNL1 CCDS1779.1 6.3e-15 29.2 SPAST CCDS1778.1 1.7e-13 27.9 SPAST The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003959 522 651 PF00004 ATPase HMMSmart IPR003593 518 654 SM00382 ATPase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060556 759 605295 100.0 100.0 XP_016859908 785 605295 100.0 98.9 XP_011509691 785 605295 100.0 98.9 NP_001308754 748 605295 100.0 98.6 NP_001274423 674 615383 38.0 97.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0058 Download

>KIEE0058  2277 bp
ATGATCAGTAGCACCAGTGTTTATGGCTTGAAGATGCAGTGGACGCCAGAGCATGCCCAG
TGGCCAGAACAGCACTTTGACATCACCTCAACCACTCGGTCTCCTGCCCACAAAGTTGAA
GCCTACAGAGGTCATCTGCAGCGCACCTATCAGTACGCCTGGGCGAATGATGACATATCT
GCTCTGACTGCATCCAACCTACTAAAAAAATATGCAGAGAAGTATTCCGGCATTTTGGAA
GGTCCTGTGGACCGACCCGTACTCAGCAACTATTCGGACACACCATCAGGACTAGTGAAC
GGTCGGAAAAATGAAAGTGAACCCTGGCAGCCTTCCTTGAATTCAGAAGCTGTTTATCCC
ATGAACTGTGTTCCGGATGTTATCACTGCCAGCAAAGCTGGAGTCAGTTCAGCCCTCCCT
CCAGCAGATGTCTCTGCGAGTATAGGAAGCTCTCCTGGGGTAGCCAGCAACCTGACAGAA
CCTAGTTATTCAAGTAGTACCTGTGGAAGCCACACTGTACCCAGTCTTCATGCAGGGCTC
CCATCTCAGGAATATGCCCCAGGATACAACGGATCATATTTGCATTCTACTTATAGTAGC
CAGCCAGCACCTGCACTTCCTTCACCTCATCCGTCTCCTTTGCATAGCTCTGGGCTACTA
CAGCCCCCACCACCACCTCCTCCGCCACCAGCCTTGGTCCCAGGCTACAATGGGACTTCT
AACCTCTCCAGTTACAGCTATCCGTCTGCTAGCTATCCTCCTCAGACTGCTGTGGGGTCT
GGGTACAGCCCTGGGGGGGCACCGCCTCCGCCTTCAGCGTACCTGCCTTCAGGAATTCCT
GCTCCCACCCCCCTACCCCCCACCACTGTTCCTGGCTACACCTACCAGGGCCATGGTTTG
ACACCTATTGCACCGTCGGCTCTGACAAACAGTTCAGCAAGTTCTCTCAAAAGGAAAGCT
TTCTACATGGCAGGGCAAGGAGATATGGACTCCAGTTATGGAAATTACAGCTATGGCCAA
CAGAGATCTACACAGAGTCCTATGTACAGAATGCCCGACAACAGCATTTCAAACACAAAT
CGGGGGAATGGCTTTGACAGAAGTGCTGAAACATCATCCTTAGCATTTAAGCCAACGAAG
CAGCTAATGTCCTCTGAACAGCAAAGGAAATTCAGCAGCCAGTCCAGTAGGGCTCTGACC
CCTCCTTCCTACAGTACTGCTAAAAATTCATTGGGATCAAGATCCAGTGAATCCTTTGGG
AAGTACACATCGCCAGTAATGAGTGAGCATGGGGACGAGCACAGGCAGCTCCTCTCTCAC
CCAATGCAAGGCCCTGGACTCCGTGCAGCTACCTCATCCAACCACTCTGTGGACGAGCAA
CTGAAGAATACTGACACGCACCTCATCGACCTGGTAACCAATGAGATTATCACCCAAGGA
CCTCCAGTGGACTGGAATGACATTGCTGGTCTCGACCTGGTGAAGGCTGTCATTAAAGAG
GAGGTTTTATGGCCAGTGTTGAGGTCAGACGCGTTCAGTGGACTGACGGCCTTACCTCGG
AGCATCCTTTTATTTGGACCTCGGGGGACAGGCAAAACATTATTGGGCAGATGCATCGCT
AGTCAGCTGGGGGCCACATTTTTCAAAATTGCCGGTTCTGGACTAGTCGCCAAGTGGTTA
GGAGAAGCAGAGAAAATTATCCATGCCTCTTTTCTTGTGGCCAGGTGTCGCCAGCCCTCG
GTGATTTTTGTTAGTGACATTGACATGCTTCTCTCCTCTCAAGTGAATGAGGAACATAGT
CCAGTCAGTCGGATGAGAACCGAATTTCTGATGCAACTGGACACTGTACTAACTTCGGCT
GAGGACCAAATCGTAGTAATTTGTGCCACCAGTAAACCAGAAGAAATAGATGAATCCCTT
CGGAGGTACTTCATGAAACGACTTTTAATCCCACTTCCTGACAGCACAGCGAGGCACCAG
ATAATAGTACAACTGCTCTCACAGCACAATTACTGTCTCAATGACAAGGAGTTTGCACTG
CTCGTCCAGCGCACAGAAGGCTTTTCTGGACTAGATGTGGCTCATTTGTGTCAGGAAGCA
GTGGTGGGCCCCCTCCATGCCATGCCAGCCACAGACCTTTCAGCCATTATGCCCAGCCAG
TTGAGGCCCGTTACATATCAAGACTTTGAAAATGCTTTCTGCAAGATTCAGCCTAGCATA
TCTCAAAAGGAGCTTGATATGTATGTTGAATGGAACAAAATGTTTGGTTGCAGTCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE0058 Download

>KIEE0058  759 aa
MISSTSVYGLKMQWTPEHAQWPEQHFDITSTTRSPAHKVEAYRGHLQRTYQYAWANDDIS
ALTASNLLKKYAEKYSGILEGPVDRPVLSNYSDTPSGLVNGRKNESEPWQPSLNSEAVYP
MNCVPDVITASKAGVSSALPPADVSASIGSSPGVASNLTEPSYSSSTCGSHTVPSLHAGL
PSQEYAPGYNGSYLHSTYSSQPAPALPSPHPSPLHSSGLLQPPPPPPPPPALVPGYNGTS
NLSSYSYPSASYPPQTAVGSGYSPGGAPPPPSAYLPSGIPAPTPLPPTTVPGYTYQGHGL
TPIAPSALTNSSASSLKRKAFYMAGQGDMDSSYGNYSYGQQRSTQSPMYRMPDNSISNTN
RGNGFDRSAETSSLAFKPTKQLMSSEQQRKFSSQSSRALTPPSYSTAKNSLGSRSSESFG
KYTSPVMSEHGDEHRQLLSHPMQGPGLRAATSSNHSVDEQLKNTDTHLIDLVTNEIITQG
PPVDWNDIAGLDLVKAVIKEEVLWPVLRSDAFSGLTALPRSILLFGPRGTGKTLLGRCIA
SQLGATFFKIAGSGLVAKWLGEAEKIIHASFLVARCRQPSVIFVSDIDMLLSSQVNEEHS
PVSRMRTEFLMQLDTVLTSAEDQIVVICATSKPEEIDESLRRYFMKRLLIPLPDSTARHQ
IIVQLLSQHNYCLNDKEFALLVQRTEGFSGLDVAHLCQEAVVGPLHAMPATDLSAIMPSQ
LRPVTYQDFENAFCKIQPSISQKELDMYVEWNKMFGCSQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0058 Download

>pFN21AE0058  6982 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATCAGTAGCACCAGTGTTTATGGCTTGAAGAT
GCAGTGGACGCCAGAGCATGCCCAGTGGCCAGAACAGCACTTTGACATCACCTCAACCAC
TCGGTCTCCTGCCCACAAAGTTGAAGCCTACAGAGGTCATCTGCAGCGCACCTATCAGTA
CGCCTGGGCGAATGATGACATATCTGCTCTGACTGCATCCAACCTACTAAAAAAATATGC
AGAGAAGTATTCCGGCATTTTGGAAGGTCCTGTGGACCGACCCGTACTCAGCAACTATTC
GGACACACCATCAGGACTAGTGAACGGTCGGAAAAATGAAAGTGAACCCTGGCAGCCTTC
CTTGAATTCAGAAGCTGTTTATCCCATGAACTGTGTTCCGGATGTTATCACTGCCAGCAA
AGCTGGAGTCAGTTCAGCCCTCCCTCCAGCAGATGTCTCTGCGAGTATAGGAAGCTCTCC
TGGGGTAGCCAGCAACCTGACAGAACCTAGTTATTCAAGTAGTACCTGTGGAAGCCACAC
TGTACCCAGTCTTCATGCAGGGCTCCCATCTCAGGAATATGCCCCAGGATACAACGGATC
ATATTTGCATTCTACTTATAGTAGCCAGCCAGCACCTGCACTTCCTTCACCTCATCCGTC
TCCTTTGCATAGCTCTGGGCTACTACAGCCCCCACCACCACCTCCTCCGCCACCAGCCTT
GGTCCCAGGCTACAATGGGACTTCTAACCTCTCCAGTTACAGCTATCCGTCTGCTAGCTA
TCCTCCTCAGACTGCTGTGGGGTCTGGGTACAGCCCTGGGGGGGCACCGCCTCCGCCTTC
AGCGTACCTGCCTTCAGGAATTCCTGCTCCCACCCCCCTACCCCCCACCACTGTTCCTGG
CTACACCTACCAGGGCCATGGTTTGACACCTATTGCACCGTCGGCTCTGACAAACAGTTC
AGCAAGTTCTCTCAAAAGGAAAGCTTTCTACATGGCAGGGCAAGGAGATATGGACTCCAG
TTATGGAAATTACAGCTATGGCCAACAGAGATCTACACAGAGTCCTATGTACAGAATGCC
CGACAACAGCATTTCAAACACAAATCGGGGGAATGGCTTTGACAGAAGTGCTGAAACATC
ATCCTTAGCATTTAAGCCAACGAAGCAGCTAATGTCCTCTGAACAGCAAAGGAAATTCAG
CAGCCAGTCCAGTAGGGCTCTGACCCCTCCTTCCTACAGTACTGCTAAAAATTCATTGGG
ATCAAGATCCAGTGAATCCTTTGGGAAGTACACATCGCCAGTAATGAGTGAGCATGGGGA
CGAGCACAGGCAGCTCCTCTCTCACCCAATGCAAGGCCCTGGACTCCGTGCAGCTACCTC
ATCCAACCACTCTGTGGACGAGCAACTGAAGAATACTGACACGCACCTCATCGACCTGGT
AACCAATGAGATTATCACCCAAGGACCTCCAGTGGACTGGAATGACATTGCTGGTCTCGA
CCTGGTGAAGGCTGTCATTAAAGAGGAGGTTTTATGGCCAGTGTTGAGGTCAGACGCGTT
CAGTGGACTGACGGCCTTACCTCGGAGCATCCTTTTATTTGGACCTCGGGGGACAGGCAA
AACATTATTGGGCAGATGCATCGCTAGTCAGCTGGGGGCCACATTTTTCAAAATTGCCGG
TTCTGGACTAGTCGCCAAGTGGTTAGGAGAAGCAGAGAAAATTATCCATGCCTCTTTTCT
TGTGGCCAGGTGTCGCCAGCCCTCGGTGATTTTTGTTAGTGACATTGACATGCTTCTCTC
CTCTCAAGTGAATGAGGAACATAGTCCAGTCAGTCGGATGAGAACCGAATTTCTGATGCA
ACTGGACACTGTACTAACTTCGGCTGAGGACCAAATCGTAGTAATTTGTGCCACCAGTAA
ACCAGAAGAAATAGATGAATCCCTTCGGAGGTACTTCATGAAACGACTTTTAATCCCACT
TCCTGACAGCACAGCGAGGCACCAGATAATAGTACAACTGCTCTCACAGCACAATTACTG
TCTCAATGACAAGGAGTTTGCACTGCTCGTCCAGCGCACAGAAGGCTTTTCTGGACTAGA
TGTGGCTCATTTGTGTCAGGAAGCAGTGGTGGGCCCCCTCCATGCCATGCCAGCCACAGA
CCTTTCAGCCATTATGCCCAGCCAGTTGAGGCCCGTTACATATCAAGACTTTGAAAATGC
TTTCTGCAAGATTCAGCCTAGCATATCTCAAAAGGAGCTTGATATGTATGTTGAATGGAA
CAAAATGTTTGGTTGCAGTCAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT