(Circular) (Six-base) MAPSORT of: pFN21AE0058.seq Check: 844 from: 1 to: 6982 pFN21AE0058 6982 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2623 2623 Size: 6982 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4792 278 Size: 53 83 186 936 3256 2468 Fragments arranged by size: 3256 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4325 1937 Size: 2388 4594 Acc65I G'GTAC_C Cuts at: 4303 4303 Size: 6982 AclI AA'CG_TT Cuts at: 1563 5109 5482 1563 Size: 3546 373 3063 Fragments arranged by size: 3546 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2802 2817 3407 5045 6429 6697 1632 Size: 1170 15 590 1638 1384 268 1917 Fragments arranged by size: 1917 1638 1384 1170 590 268 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6785 AflIII A'CryG_T Cuts at: 1203 3534 5881 6858 1203 Size: 2331 2347 977 1327 Fragments arranged by size: 2347 2331 1327 977 AgeI A'CCGG_T Cuts at: 5813 5813 Size: 6982 AhdI GACnn_n'nnGTC Cuts at: 5711 5711 Size: 6982 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6982 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6982 AlwNI CAG_nnn'CTG Cuts at: 2354 2615 4725 6297 2354 Size: 261 2110 1572 3039 Fragments arranged by size: 3039 2110 1572 261 ApaI G_GGCC'C Cuts at: 4115 4115 Size: 6982 ApaLI G'TGCA_C Cuts at: 5039 6195 5039 Size: 1156 5826 ApoI r'AATT_y Cuts at: 1493 2344 2838 3173 3229 3827 4291 4517 4669 Size: 851 494 335 56 598 464 226 152 Cuts at: 4669 6778 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 851 598 494 464 335 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 5536 160 Size: 872 4504 1606 Fragments arranged by size: 4504 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6982 AvaI C'yCGr_G Cuts at: 1870 1943 3585 4307 1870 Size: 73 1642 722 4545 Fragments arranged by size: 4545 1642 722 73 BamHI G'GATC_C Cuts at: 4312 4679 4312 Size: 367 6615 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2803 3978 4303 5758 618 Size: 325 217 627 1016 1175 325 1455 1842 Fragments arranged by size: 1842 1455 1175 1016 627 325 325 217 BanII G_rGCy'C Cuts at: 1391 2544 3199 4115 4301 1391 Size: 1153 655 916 186 4072 Fragments arranged by size: 4072 1153 916 655 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2522 928 Size: 971 623 5388 Fragments arranged by size: 5388 971 623 BbvCI CC'TCA_GC Cuts at: 3864 3864 Size: 6982 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3601 3635 5173 5207 1903 Size: 34 1664 34 1538 34 3678 Fragments arranged by size: 3678 1664 1538 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2561 4874 6084 2561 Size: 2313 1210 3459 Fragments arranged by size: 3459 2313 1210 BclI T'GATC_A Cuts at: 1548 2007 1548 Size: 459 6523 BfrBI ATG'CAT Cuts at: 3618 3618 Size: 6982 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5593 136 Size: 107 122 71 631 605 3921 1525 Fragments arranged by size: 3921 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3029 6977 3029 Size: 3948 3034 BlpI GC'TnA_GC Cuts at: 4399 4399 Size: 6982 Bme1580I G_kGCm'C Cuts at: 1790 2806 4115 5043 6199 1790 Size: 1016 1309 928 1156 2573 Fragments arranged by size: 2573 1309 1156 1016 928 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6982 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2056 2520 4713 Size: 416 53 83 399 24 639 464 2193 Cuts at: 4713 5671 442 Size: 958 1753 Fragments arranged by size: 2193 1753 958 639 464 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2757 1056 Size: 1701 5281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2409 2424 2716 2980 3432 5642 1239 Size: 1170 15 292 264 452 2210 2579 Fragments arranged by size: 2579 2210 1170 452 292 264 15 Bpu10I CC'TnA_GC Cuts at: 3124 3864 3124 Size: 740 6242 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3768 5107 5972 6270 3768 Size: 1339 865 298 4480 Fragments arranged by size: 4480 1339 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2109 3479 5645 882 Size: 617 610 1370 2166 2219 Fragments arranged by size: 2219 2166 1370 617 610 BsaAI yAC'GTr Cuts at: 493 1204 4773 493 Size: 711 3569 2702 Fragments arranged by size: 3569 2702 711 BsaBI GATnn'nnATC Cuts at: 3244 6976 3244 Size: 3732 3250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 4789 5171 5806 Size: 53 83 186 936 515 2741 382 635 Cuts at: 5806 275 Size: 1451 Fragments arranged by size: 2741 1451 936 635 515 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2377 5415 5813 6087 6234 6645 2377 Size: 3038 398 274 147 411 2714 Fragments arranged by size: 3038 2714 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2089 2119 1178 Size: 30 165 30 686 30 6041 Fragments arranged by size: 6041 686 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2673 2754 3305 3518 3769 1186 Size: 1487 81 551 213 251 4399 Fragments arranged by size: 4399 1487 551 251 213 81 BseYI C'CCAG_C Cuts at: 3631 4157 6185 6681 3631 Size: 526 2028 496 3932 Fragments arranged by size: 3932 2028 526 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2601 3369 1163 Size: 573 865 768 4776 Fragments arranged by size: 4776 865 768 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2308 4432 5193 6221 1540 Size: 275 186 307 2124 761 1028 2301 Fragments arranged by size: 2301 2124 1028 761 307 275 186 BsiHKAI G_wGCw'C Cuts at: 3306 5043 5128 6199 3306 Size: 1737 85 1071 4089 Fragments arranged by size: 4089 1737 1071 85 BsmI GAATG_Cn' Cuts at: 2587 3060 4493 4586 2587 Size: 473 1433 93 4983 Fragments arranged by size: 4983 1433 473 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2643 2643 Size: 6982 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2544 2806 3199 3306 4115 4301 5043 Size: 399 754 262 393 107 809 186 742 Cuts at: 5043 5128 6199 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 809 754 742 399 393 262 186 107 85 BspEI T'CCGG_A Cuts at: 2377 6645 2377 Size: 4268 2714 BspHI T'CATG_A Cuts at: 3936 4871 6601 3936 Size: 935 1730 4317 Fragments arranged by size: 4317 1730 935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2623 2835 4337 844 Size: 1779 212 1502 3489 Fragments arranged by size: 3489 1779 1502 212 BsrBI CCG'CTC Cuts at: 1394 1823 4869 1394 Size: 429 3046 3507 Fragments arranged by size: 3507 3046 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3465 5477 5651 65 Size: 1183 2217 2012 174 1396 Fragments arranged by size: 2217 2012 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3656 5626 5813 1700 Size: 110 1846 1970 187 2869 Fragments arranged by size: 2869 1970 1846 187 110 BsrGI T'GTAC_A Cuts at: 96 3048 96 Size: 2952 4030 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6982 BssSI C'ACGA_G Cuts at: 5042 6054 5042 Size: 1012 5970 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6982 BstBI TT'CG_AA Cuts at: 5786 5786 Size: 6982 BstEII G'GTnAC_C Cuts at: 1184 3418 1184 Size: 2234 4748 BstXI CCAn_nnnn'nTGG Cuts at: 2897 3456 2897 Size: 559 6423 BstYI r'GATC_y Cuts at: 1972 3029 3245 4312 4679 5064 5081 6533 6619 Size: 1057 216 1067 367 385 17 1452 86 Cuts at: 6619 6631 6977 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1067 1057 385 367 346 216 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2896 513 Size: 552 1831 4599 Fragments arranged by size: 4599 1831 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3258 3604 487 Size: 683 56 2032 346 3865 Fragments arranged by size: 3865 2032 683 346 56 BtsI GCAGTG_nn' Cuts at: 2048 2388 4040 4110 4498 5292 5312 2048 Size: 340 1652 70 388 794 20 3718 Fragments arranged by size: 3718 1652 794 388 340 70 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6982 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2679 2714 529 Size: 35 2115 35 4797 Fragments arranged by size: 4797 2115 35 35 DraI TTT'AAA Cuts at: 4286 4286 Size: 6982 DraIII CAC_nnn'GTG Cuts at: 3373 3373 Size: 6982 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5989 809 Size: 507 4673 1802 Fragments arranged by size: 4673 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 3019 3515 Size: 54 1425 50 275 18 236 953 496 Cuts at: 3515 3723 4429 5318 6947 8 Size: 208 706 889 1629 43 Fragments arranged by size: 1629 1425 953 889 706 496 275 236 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4429 1537 Size: 275 2617 4090 Fragments arranged by size: 4090 2617 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4912 1353 Size: 3559 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2676 2802 5569 5943 6089 387 Size: 1064 138 1087 126 2767 374 146 1280 Fragments arranged by size: 2767 1280 1087 1064 374 146 138 126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2409 2424 2716 2802 2817 2980 3407 Size: 393 777 15 292 86 15 163 427 Cuts at: 3407 3432 5045 5642 6429 6697 1239 Size: 25 1613 597 787 268 1524 Fragments arranged by size: 1613 1524 787 777 597 427 393 292 268 163 86 25 15 15 EcoNI CCTnn'n_nnAGG Cuts at: 3485 3485 Size: 6982 EcoO109I rG'GnC_Cy Cuts at: 2246 3335 3443 4112 2246 Size: 1089 108 669 5116 Fragments arranged by size: 5116 1089 669 108 EcoRI G'AATT_C Cuts at: 2344 2838 4291 4669 2344 Size: 494 1453 378 4657 Fragments arranged by size: 4657 1453 494 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2979 2979 Size: 6982 FspI TGC'GCA Cuts at: 5488 5488 Size: 6982 HaeII r_GCGC'y Cuts at: 1678 6129 1678 Size: 4451 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2165 2197 2250 Size: 32 135 32 503 32 58 32 53 Cuts at: 2250 2273 2282 2305 2978 3010 3936 3968 5623 Size: 23 9 23 673 32 926 32 1655 Cuts at: 5623 5655 5697 5729 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1655 926 673 503 135 58 53 42 32 32 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 4326 4572 669 Size: 988 281 2388 246 3079 Fragments arranged by size: 3079 2388 988 281 246 HindIII A'AGCT_T Cuts at: 748 2961 748 Size: 2213 4769 HpaI GTT'AAC Cuts at: 4572 4572 Size: 6982 KpnI G_GTAC'C Cuts at: 4307 4307 Size: 6982 MfeI C'AATT_G Cuts at: 4581 4581 Size: 6982 MluI A'CGCG_T Cuts at: 3534 6858 3534 Size: 3324 3658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2223 2288 3387 3794 6096 6280 1305 Size: 918 65 1099 407 2302 184 2007 Fragments arranged by size: 2302 2007 1099 918 407 184 65 MscI TGG'CCA Cuts at: 10 64 1489 1832 2068 3021 3517 3725 10 Size: 54 1425 343 236 953 496 208 3267 Fragments arranged by size: 3267 1425 953 496 343 236 208 54 MslI CAynn'nnrTG Cuts at: 518 1166 3767 4940 5299 5458 518 Size: 648 2601 1173 359 159 2042 Fragments arranged by size: 2601 2042 1173 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3631 4398 5075 6223 6468 1606 Size: 2025 767 677 1148 245 2120 Fragments arranged by size: 2120 2025 1148 767 677 245 NcoI C'CATG_G Cuts at: 513 1065 2896 513 Size: 552 1831 4599 Fragments arranged by size: 4599 1831 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6982 NheI G'CTAG_C Cuts at: 1052 2753 1052 Size: 1701 5281 NotI GC'GGCC_GC Cuts at: 4429 4429 Size: 6982 NsiI A_TGCA'T Cuts at: 3620 3620 Size: 6982 NspI r_CATG'y Cuts at: 1132 1229 2060 3772 4340 5885 1132 Size: 97 831 1712 568 1545 2229 Fragments arranged by size: 2229 1712 1545 831 568 97 PasI CC'CwG_GG Cuts at: 1348 2795 1348 Size: 1447 5535 PciI A'CATG_T Cuts at: 5881 5881 Size: 6982 PflMI CCAn_nnn'nTGG Cuts at: 1108 3373 1108 Size: 2265 4717 PmeI GTTT'AAAC Cuts at: 4286 4286 Size: 6982 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5051 5083 6564 6596 5051 Size: 32 1481 32 5437 Fragments arranged by size: 5437 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2246 3443 2246 Size: 1197 5785 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6982 PsiI TTA'TAA Cuts at: 4552 4552 Size: 6982 PspOMI G'GGCC_C Cuts at: 4111 4111 Size: 6982 PstI C_TGCA'G Cuts at: 830 2145 4334 830 Size: 1315 2189 3478 Fragments arranged by size: 3478 2189 1315 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6982 PvuII CAG'CTG Cuts at: 3631 3631 Size: 6982 SalI G'TCGA_C Cuts at: 1936 4324 1936 Size: 2388 4594 SbfI CC_TGCA'GG Cuts at: 4334 4334 Size: 6982 ScaI AGT'ACT Cuts at: 3220 5230 6942 3220 Size: 2010 1712 3260 Fragments arranged by size: 3260 2010 1712 SexAI A'CCwGG_T Cuts at: 3414 3480 3414 Size: 66 6916 SfcI C'TryA_G Cuts at: 826 1045 2128 2141 2663 3214 4330 5465 6146 Size: 219 1083 13 522 551 1116 1135 681 Cuts at: 6146 6337 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1116 1083 681 551 522 219 191 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6982 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6982 SmaI CCC'GGG Cuts at: 1872 4309 1872 Size: 2437 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3783 5086 5987 6249 820 Size: 197 926 1840 1303 901 262 1553 Fragments arranged by size: 1840 1553 1303 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4773 493 Size: 4280 2702 SpeI A'CTAG_T Cuts at: 152 2296 3667 152 Size: 2144 1371 3467 Fragments arranged by size: 3467 2144 1371 SphI G_CATG'C Cuts at: 1132 2060 4340 1132 Size: 928 2280 3774 Fragments arranged by size: 3774 2280 928 SspI AAT'ATT Cuts at: 5 52 4906 5 Size: 47 4854 2081 Fragments arranged by size: 4854 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6982 StyI C'CwwG_G Cuts at: 513 1065 2697 2896 3439 4421 513 Size: 552 1632 199 543 982 3074 Fragments arranged by size: 3074 1632 982 552 543 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2091 2271 3339 3452 5009 5026 5179 Size: 1255 511 180 1068 113 1557 17 153 Cuts at: 5179 5364 5809 325 Size: 185 445 1498 Fragments arranged by size: 1557 1498 1255 1068 511 445 185 180 153 113 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3048 3218 3267 Size: 274 80 33 51 1443 1071 170 49 Cuts at: 3267 3850 3991 5228 6940 96 Size: 583 141 1237 1712 138 Fragments arranged by size: 1712 1443 1237 1071 583 274 170 141 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2089 2121 2857 2889 3435 3467 4032 4064 4065 Size: 32 736 32 546 32 565 32 1 Cuts at: 4065 4097 6942 6974 2089 Size: 32 2845 32 2097 Fragments arranged by size: 2845 2097 736 565 546 32 32 32 32 32 32 1 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6982 XbaI T'CTAG_A Cuts at: 4318 4318 Size: 6982 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3370 3370 Size: 6982 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6982 XmaI C'CCGG_G Cuts at: 1870 4307 1870 Size: 2437 4545 XmnI GAAnn'nnTTC Cuts at: 2963 3921 5111 2963 Size: 958 1190 4834 Fragments arranged by size: 4834 1190 958 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4790 276 Size: 53 83 186 936 3256 2468 Fragments arranged by size: 3256 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI