MTIF2 ORF clone pFN21AE0049

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0049

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Clone info.

Species

human

Clone Name

pFN21AE0049 Sequence (with vector) Restriction Map

Product ID

FHC10784

Flexi Clone PID

FXC10784

ORF Sequence

DNA sequence (2181 bp)
Protein sequence (727 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587520]

Gene

KIEE0049

Symbol

MTIF2

Description

mitochondrial translational initiation factor 2, transcript variant 2

Original Clone ID

oc00749

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2181 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 727 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1853.1 0 100.0 MTIF2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000795 181 341 PF00009 Protein synthesis factor IPR004161 369 429 PF03144 Translation elongation factor EFTu/EF1A IPR023115 510 607 PF11987 Translation initiation factor IF- 2 HMMTigr IPR005225 181 304 TIGR00231 Small GTP-binding protein domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001307932 727 603766 100.0 100.0 NP_001307933 727 603766 100.0 100.0 NP_001307931 727 603766 100.0 100.0 NP_001005369 727 603766 100.0 100.0 NP_001307930 727 603766 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0049 Download

>KIEE0049  2181 bp
ATGAACCAGAAGCTACTGAAGTTGGAGAACTTGCTACGATTTCACACTATTTATAGGCAA
CTGCACAGTCTGTGTCAAAGAAGAGCATTAAGACAGTGGAGGCATGGGTTTTCATCTGCT
TACCCTGTGTGGACAGCTCAACTGTGTGCCTGGCCCTGGCCAACAGATGTGCTCACTGGG
GCTGCTTTATCTCAGTATAGGCTTCTAGTAACAAAAAAGGAAGAAGGACCATGGAAATCT
CAGTTATCTTCAACAAAATCTAAAAAGGTGGTAGAAGTATGGATTGGAATGACTATTGAG
GAACTGGCCAGGGCAATGGAAAAAAACACAGATTATGTATATGAAGCTTTATTGAACACT
GATATTGACATAGATTCACTGGAAGCAGACTCACATTTAGATGAAGTCTGGATCAAAGAA
GTGATAACGAAGGCAGGGATGAAGTTAAAGTGGAGTAAATTAAAACAGGACAAAGTCAGA
AAAAATAAAGATGCTGTAAGAAGGCCCCAGGCAGATCCAGCTTTATTAACCCCAAGGTCC
CCAGTTGTTACTATAATGGGCCATGTTGATCACGGGAAAACGACATTACTTGACAAATTT
CGAAAAACTCAAGTGGCAGCAGTGGAAACTGGAGGCATCACTCAGCACATTGGTGCCTTT
CTTGTCTCTCTGCCTTCTGGGGAAAAGATAACTTTTCTTGATACTCCAGGACATGCTGCT
TTCTCAGCAATGAGAGCCAGAGGTGCTCAGGTCACTGACATTGTCGTATTGGTTGTAGCT
GCAGATGATGGAGTGATGAAACAAACTGTAGAATCTATTCAGCATGCCAAAGATGCACAG
GTTCCTATTATCCTTGCCGTAAATAAATGTGACAAAGCTGAGGCTGATCCTGAGAAAGTG
AAAAAAGAGCTGCTGGCTTACGATGTGGTATGTGAAGATTATGGAGGTGATGTTCAAGCA
GTGCCTGTCTCCGCACTTACGGGCGATAATCTGATGGCTTTGGCAGAAGCAACAGTTGCT
CTTGCAGAAATGTTAGAATTGAAAGCAGATCCCAATGGTCCAGTGGAAGGAACAGTAATA
GAGTCTTTCACAGACAAAGGAAGAGGTCTTGTTACTACAGCTATAATTCAAAGAGGAACT
TTAAGAAAAGGCTCTGTTCTGGTTGCTGGAAAATGTTGGGCAAAAGTACGCTTAATGTTT
GATGAAAATGGAAAAACAATTGATGAGGCCTATCCCAGCATGCCAGTGGGAATTACAGGC
TGGAGAGACCTTCCTTCTGCAGGAGAAGAAATTCTTGAAGTAGAATCTGAGCCAAGGGCA
CGTGAAGTTGTTGACTGGAGGAAATATGAACAAGAACAGGAGAAAGGTCAGGAGGATCTG
AAAATAATAGAAGAAAAGCGAAAGGAACACAAAGAAGCACATCAGAAAGCCCGTGAGAAG
TATGGCCATCTACTGTGGAAGAAGAGATCAATTCTACGGTTTTTAGAAAGAAAAGAACAA
ATACCCTTAAAGCCAAAAGAGAAAAGGGAAAGAGATTCAAATGTACTTTCTGTGATTATT
AAAGGTGATGTTGATGGTTCTGTTGAGGCCATTTTGAACATTATAGATACCTATGATGCT
TCACACGAGTGTGAACTAGAATTAGTACATTTTGGAGTGGGTGATGTAAGTGCAAATGAT
GTTAACCTTGCTGAAACATTTGATGGTGTTATATATGGCTTTAATGTGAATGCAGGCAAT
GTTATCCAACAGTCAGCTGCAAAAAAAGGAGTAAAAATTAAACTTCACAAAATAATTTAC
CGTCTTGTTGAAGATTTGCAAGAGGAACTGAGCAGCAGATTACCCTGTGCTGTGGAAGAG
CACCCAGTAGGTGAGGCATCTATACTAGCTACCTTCTCTGTAACAGAAGGGAAGAAAAAA
GTTCCTGTGGCTGGCTGCAGAGTCCAAAAGGGACAGTTAGAAAAACAAAAAAAATTTAAA
CTAACCCGTAATGGACATGTAATTTGGAAGGGCTCATTAACCTCATTGAAACACCATAAA
GATGACATTTCAATTGTCAAAACGGGAATGGATTGTGGTCTCAGTTTAGATGAAGACAAT
ATGGAATTTCAAGTGGGAGACAGAATTGTTTGTTATGAAGAAAAGCAAATTCAAGCCAAG
ACTTCTTGGGATCCAGGATTT

ORF Protein

Cloned ORF protein sequence for pFN21AE0049 Download

>KIEE0049  727 aa
MNQKLLKLENLLRFHTIYRQLHSLCQRRALRQWRHGFSSAYPVWTAQLCAWPWPTDVLTG
AALSQYRLLVTKKEEGPWKSQLSSTKSKKVVEVWIGMTIEELARAMEKNTDYVYEALLNT
DIDIDSLEADSHLDEVWIKEVITKAGMKLKWSKLKQDKVRKNKDAVRRPQADPALLTPRS
PVVTIMGHVDHGKTTLLDKFRKTQVAAVETGGITQHIGAFLVSLPSGEKITFLDTPGHAA
FSAMRARGAQVTDIVVLVVAADDGVMKQTVESIQHAKDAQVPIILAVNKCDKAEADPEKV
KKELLAYDVVCEDYGGDVQAVPVSALTGDNLMALAEATVALAEMLELKADPNGPVEGTVI
ESFTDKGRGLVTTAIIQRGTLRKGSVLVAGKCWAKVRLMFDENGKTIDEAYPSMPVGITG
WRDLPSAGEEILEVESEPRAREVVDWRKYEQEQEKGQEDLKIIEEKRKEHKEAHQKAREK
YGHLLWKKRSILRFLERKEQIPLKPKEKRERDSNVLSVIIKGDVDGSVEAILNIIDTYDA
SHECELELVHFGVGDVSANDVNLAETFDGVIYGFNVNAGNVIQQSAAKKGVKIKLHKIIY
RLVEDLQEELSSRLPCAVEEHPVGEASILATFSVTEGKKKVPVAGCRVQKGQLEKQKKFK
LTRNGHVIWKGSLTSLKHHKDDISIVKTGMDCGLSLDEDNMEFQVGDRIVCYEEKQIQAK
TSWDPGF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0049 Download

>pFN21AE0049  6886 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACCAGAAGCTACTGAAGTTGGAGAACTTGCT
ACGATTTCACACTATTTATAGGCAACTGCACAGTCTGTGTCAAAGAAGAGCATTAAGACA
GTGGAGGCATGGGTTTTCATCTGCTTACCCTGTGTGGACAGCTCAACTGTGTGCCTGGCC
CTGGCCAACAGATGTGCTCACTGGGGCTGCTTTATCTCAGTATAGGCTTCTAGTAACAAA
AAAGGAAGAAGGACCATGGAAATCTCAGTTATCTTCAACAAAATCTAAAAAGGTGGTAGA
AGTATGGATTGGAATGACTATTGAGGAACTGGCCAGGGCAATGGAAAAAAACACAGATTA
TGTATATGAAGCTTTATTGAACACTGATATTGACATAGATTCACTGGAAGCAGACTCACA
TTTAGATGAAGTCTGGATCAAAGAAGTGATAACGAAGGCAGGGATGAAGTTAAAGTGGAG
TAAATTAAAACAGGACAAAGTCAGAAAAAATAAAGATGCTGTAAGAAGGCCCCAGGCAGA
TCCAGCTTTATTAACCCCAAGGTCCCCAGTTGTTACTATAATGGGCCATGTTGATCACGG
GAAAACGACATTACTTGACAAATTTCGAAAAACTCAAGTGGCAGCAGTGGAAACTGGAGG
CATCACTCAGCACATTGGTGCCTTTCTTGTCTCTCTGCCTTCTGGGGAAAAGATAACTTT
TCTTGATACTCCAGGACATGCTGCTTTCTCAGCAATGAGAGCCAGAGGTGCTCAGGTCAC
TGACATTGTCGTATTGGTTGTAGCTGCAGATGATGGAGTGATGAAACAAACTGTAGAATC
TATTCAGCATGCCAAAGATGCACAGGTTCCTATTATCCTTGCCGTAAATAAATGTGACAA
AGCTGAGGCTGATCCTGAGAAAGTGAAAAAAGAGCTGCTGGCTTACGATGTGGTATGTGA
AGATTATGGAGGTGATGTTCAAGCAGTGCCTGTCTCCGCACTTACGGGCGATAATCTGAT
GGCTTTGGCAGAAGCAACAGTTGCTCTTGCAGAAATGTTAGAATTGAAAGCAGATCCCAA
TGGTCCAGTGGAAGGAACAGTAATAGAGTCTTTCACAGACAAAGGAAGAGGTCTTGTTAC
TACAGCTATAATTCAAAGAGGAACTTTAAGAAAAGGCTCTGTTCTGGTTGCTGGAAAATG
TTGGGCAAAAGTACGCTTAATGTTTGATGAAAATGGAAAAACAATTGATGAGGCCTATCC
CAGCATGCCAGTGGGAATTACAGGCTGGAGAGACCTTCCTTCTGCAGGAGAAGAAATTCT
TGAAGTAGAATCTGAGCCAAGGGCACGTGAAGTTGTTGACTGGAGGAAATATGAACAAGA
ACAGGAGAAAGGTCAGGAGGATCTGAAAATAATAGAAGAAAAGCGAAAGGAACACAAAGA
AGCACATCAGAAAGCCCGTGAGAAGTATGGCCATCTACTGTGGAAGAAGAGATCAATTCT
ACGGTTTTTAGAAAGAAAAGAACAAATACCCTTAAAGCCAAAAGAGAAAAGGGAAAGAGA
TTCAAATGTACTTTCTGTGATTATTAAAGGTGATGTTGATGGTTCTGTTGAGGCCATTTT
GAACATTATAGATACCTATGATGCTTCACACGAGTGTGAACTAGAATTAGTACATTTTGG
AGTGGGTGATGTAAGTGCAAATGATGTTAACCTTGCTGAAACATTTGATGGTGTTATATA
TGGCTTTAATGTGAATGCAGGCAATGTTATCCAACAGTCAGCTGCAAAAAAAGGAGTAAA
AATTAAACTTCACAAAATAATTTACCGTCTTGTTGAAGATTTGCAAGAGGAACTGAGCAG
CAGATTACCCTGTGCTGTGGAAGAGCACCCAGTAGGTGAGGCATCTATACTAGCTACCTT
CTCTGTAACAGAAGGGAAGAAAAAAGTTCCTGTGGCTGGCTGCAGAGTCCAAAAGGGACA
GTTAGAAAAACAAAAAAAATTTAAACTAACCCGTAATGGACATGTAATTTGGAAGGGCTC
ATTAACCTCATTGAAACACCATAAAGATGACATTTCAATTGTCAAAACGGGAATGGATTG
TGGTCTCAGTTTAGATGAAGACAATATGGAATTTCAAGTGGGAGACAGAATTGTTTGTTA
TGAAGAAAAGCAAATTCAAGCCAAGACTTCTTGGGATCCAGGATTTGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT