(Circular) (Six-base) MAPSORT of: pFN21AE0049.seq Check: 6830 from: 1 to: 6886 pFN21AE0049 6886 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4696 278 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4229 1937 Size: 2292 4594 Acc65I G'GTAC_C Cuts at: 4207 4207 Size: 6886 AclI AA'CG_TT Cuts at: 1563 5013 5386 1563 Size: 3450 373 3063 Fragments arranged by size: 3450 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 4949 6333 6601 1632 Size: 410 2907 1384 268 1917 Fragments arranged by size: 2907 1917 1384 410 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 4000 5785 6762 1203 Size: 2797 1785 977 1327 Fragments arranged by size: 2797 1785 1327 977 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 5615 5615 Size: 6886 AleI CACnn'nnGTG Cuts at: 1166 2655 3631 3633 1166 Size: 1489 976 2 4419 Fragments arranged by size: 4419 1489 976 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2673 1184 Size: 1489 5397 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2940 2972 2940 Size: 32 6854 AlwNI CAG_nnn'CTG Cuts at: 2969 4629 6201 2969 Size: 1660 1572 3654 Fragments arranged by size: 3654 1660 1572 ApaLI G'TGCA_C Cuts at: 4943 6099 4943 Size: 1156 5730 ApoI r'AATT_y Cuts at: 1493 2600 3294 3977 4109 4152 4195 4421 4573 Size: 1107 694 683 132 43 43 226 152 Cuts at: 4573 6682 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1107 694 683 226 152 132 43 43 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 4211 1870 Size: 73 2268 4545 Fragments arranged by size: 4545 2268 73 BamHI G'GATC_C Cuts at: 4174 4216 4583 4174 Size: 42 367 6477 Fragments arranged by size: 6477 367 42 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2657 4207 5662 618 Size: 325 217 627 870 1550 1455 1842 Fragments arranged by size: 1842 1550 1455 870 627 325 217 BanII G_rGCy'C Cuts at: 1391 4019 4205 1391 Size: 2628 186 4072 Fragments arranged by size: 4072 2628 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4104 928 Size: 971 2205 3710 Fragments arranged by size: 3710 2205 971 BbvCI CC'TCA_GC Cuts at: 2883 2883 Size: 6886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5077 5111 1903 Size: 34 3140 34 3678 Fragments arranged by size: 3678 3140 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4778 5988 4778 Size: 1210 5676 BclI T'GATC_A Cuts at: 1548 2572 1548 Size: 1024 5862 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5497 136 Size: 107 122 71 631 605 3825 1525 Fragments arranged by size: 3825 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6881 6881 Size: 6886 BlpI GC'TnA_GC Cuts at: 4303 4303 Size: 6886 Bme1580I G_kGCm'C Cuts at: 1790 3325 4947 6103 1790 Size: 1535 1622 1156 2573 Fragments arranged by size: 2573 1622 1535 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6886 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2190 2540 3863 Size: 416 53 83 399 24 773 350 1323 Cuts at: 3863 4617 5575 442 Size: 754 958 1753 Fragments arranged by size: 1753 1323 958 773 754 416 399 350 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2655 2694 3286 3361 5546 1239 Size: 1416 39 592 75 2185 2579 Fragments arranged by size: 2579 2185 1416 592 75 39 Bpu10I CC'TnA_GC Cuts at: 2751 2883 2751 Size: 132 6754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2598 5011 5876 6174 2598 Size: 2413 865 298 3310 Fragments arranged by size: 3310 2413 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3264 4088 5549 882 Size: 617 1765 824 1461 2219 Fragments arranged by size: 2219 1765 1461 824 617 BsaAI yAC'GTr Cuts at: 493 1204 3326 4677 493 Size: 711 2122 1351 2702 Fragments arranged by size: 2702 2122 1351 711 BsaBI GATnn'nnATC Cuts at: 6880 6880 Size: 6886 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4693 5075 5710 275 Size: 53 83 186 936 3160 382 635 1451 Fragments arranged by size: 3160 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5319 5717 5991 6138 6549 5319 Size: 398 274 147 411 5656 Fragments arranged by size: 5656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2940 2970 1178 Size: 30 165 30 1537 30 5094 Fragments arranged by size: 5094 1537 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6886 BseYI C'CCAG_C Cuts at: 3239 6089 6585 3239 Size: 2850 496 3540 Fragments arranged by size: 3540 2850 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 1163 Size: 573 315 5998 Fragments arranged by size: 5998 573 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4336 5097 6125 1540 Size: 275 186 2335 761 1028 2301 Fragments arranged by size: 2335 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2178 2752 3867 4947 5032 6103 2178 Size: 574 1115 1080 85 1071 2961 Fragments arranged by size: 2961 1115 1080 1071 574 85 BsmI GAATG_Cn' Cuts at: 3739 4397 4490 3739 Size: 658 93 6135 Fragments arranged by size: 6135 658 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 2752 3325 3867 4019 4205 4947 Size: 399 388 574 573 542 152 186 742 Cuts at: 4947 5032 6103 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 574 573 542 399 388 186 152 85 BspEI T'CCGG_A Cuts at: 6549 6549 Size: 6886 BspHI T'CATG_A Cuts at: 4775 6505 4775 Size: 1730 5156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4241 844 Size: 3397 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4773 1394 Size: 429 2950 3507 Fragments arranged by size: 3507 2950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2325 2739 3748 5381 5555 65 Size: 1183 1077 414 1009 1633 174 1396 Fragments arranged by size: 1633 1396 1183 1077 1009 414 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5530 5717 1700 Size: 110 3720 187 2869 Fragments arranged by size: 3720 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 3629 4946 5958 3629 Size: 1317 1012 4557 Fragments arranged by size: 4557 1317 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3020 794 Size: 2226 4660 BstBI TT'CG_AA Cuts at: 2605 5690 2605 Size: 3085 3801 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6886 BstXI CCAn_nnnn'nTGG Cuts at: 2320 2320 Size: 6886 BstYI r'GATC_y Cuts at: 1972 2518 3052 3379 4174 4216 4583 4968 4985 Size: 546 534 327 795 42 367 385 17 Cuts at: 4985 6437 6523 6535 6881 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 795 546 534 385 367 346 327 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2234 513 Size: 552 1169 5165 Fragments arranged by size: 5165 1169 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6147 Fragments arranged by size: 6147 683 56 BtsI GCAGTG_nn' Cuts at: 2631 2970 4402 5196 5216 2631 Size: 339 1432 794 20 4301 Fragments arranged by size: 4301 1432 794 339 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6851 DraI TTT'AAA Cuts at: 3982 4190 3982 Size: 208 6678 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2162 2310 3448 Size: 54 1425 50 275 18 332 148 1138 Cuts at: 3448 4333 5222 6851 8 Size: 885 889 1629 43 Fragments arranged by size: 1629 1425 1138 889 885 332 275 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2080 3100 3461 3855 4816 1353 Size: 727 1020 361 394 961 3423 Fragments arranged by size: 3423 1020 961 727 394 361 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5473 5847 5993 387 Size: 1064 138 3884 374 146 1280 Fragments arranged by size: 3884 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2655 2694 3286 3361 4949 5546 Size: 393 410 613 39 592 75 1588 597 Cuts at: 5546 6333 6601 1239 Size: 787 268 1524 Fragments arranged by size: 1588 1524 787 613 597 592 410 393 268 75 39 EcoNI CCTnn'n_nnAGG Cuts at: 3282 3282 Size: 6886 EcoO109I rG'GnC_Cy Cuts at: 2508 2541 2508 Size: 33 6853 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3159 3314 3159 Size: 155 6731 FspI TGC'GCA Cuts at: 5392 5392 Size: 6886 HaeII r_GCGC'y Cuts at: 1678 6033 1678 Size: 4355 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5527 5559 5601 5633 1373 Size: 32 135 32 3955 32 42 32 2626 Fragments arranged by size: 3955 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3337 3688 4230 4476 669 Size: 988 281 1399 351 542 246 3079 Fragments arranged by size: 3079 1399 988 542 351 281 246 HindIII A'AGCT_T Cuts at: 748 2349 748 Size: 1601 5285 HpaI GTT'AAC Cuts at: 3688 4476 3688 Size: 788 6098 KpnI G_GTAC'C Cuts at: 4211 4211 Size: 6886 MfeI C'AATT_G Cuts at: 3222 4056 4485 3222 Size: 834 429 5623 Fragments arranged by size: 5623 834 429 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 3775 6000 6184 1305 Size: 702 1768 2225 184 2007 Fragments arranged by size: 2225 2007 1768 702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2164 2312 3450 10 Size: 54 1425 343 332 148 1138 3446 Fragments arranged by size: 3446 1425 1138 343 332 148 54 MslI CAynn'nnrTG Cuts at: 518 1166 2403 2655 3248 3631 3633 4044 4844 Size: 648 1237 252 593 383 2 411 800 Cuts at: 4844 5203 5362 518 Size: 359 159 2042 Fragments arranged by size: 2042 1237 800 648 593 411 383 359 252 159 2 MspA1I CmG'CkG Cuts at: 1606 3761 4302 4979 6127 6372 1606 Size: 2155 541 677 1148 245 2120 Fragments arranged by size: 2155 2120 1148 677 541 245 NcoI C'CATG_G Cuts at: 513 1065 2234 513 Size: 552 1169 5165 Fragments arranged by size: 5165 1169 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 2720 2831 3247 4004 4244 5789 1132 Size: 97 1491 111 416 757 240 1545 2229 Fragments arranged by size: 2229 1545 1491 757 416 240 111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6886 PciI A'CATG_T Cuts at: 4000 5785 4000 Size: 1785 5101 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6886 PfoI T'CCnGG_A Cuts at: 2710 4177 2710 Size: 1467 5419 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PmlI CAC'GTG Cuts at: 3326 3326 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2940 2972 4955 4987 6468 6500 2940 Size: 32 1983 32 1481 32 3326 Fragments arranged by size: 3326 1983 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2541 2541 Size: 6886 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6886 PsiI TTA'TAA Cuts at: 4456 4456 Size: 6886 PstI C_TGCA'G Cuts at: 830 2788 3286 3944 4238 830 Size: 1958 498 658 294 3478 Fragments arranged by size: 3478 1958 658 498 294 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6886 PvuII CAG'CTG Cuts at: 3761 3761 Size: 6886 SalI G'TCGA_C Cuts at: 1936 4228 1936 Size: 2292 4594 SapI GCTCTTCn'nnn_ Cuts at: 2080 3855 2080 Size: 1775 5111 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 5134 6846 5134 Size: 1712 5174 SfcI C'TryA_G Cuts at: 826 1045 2784 2811 3120 3282 3940 4234 5369 Size: 219 1739 27 309 162 658 294 1135 Cuts at: 5369 6050 6241 826 Size: 681 191 1471 Fragments arranged by size: 1739 1471 1135 681 658 309 294 219 191 162 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 4213 1872 Size: 2341 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2613 4990 5891 6153 820 Size: 197 926 670 2377 901 262 1553 Fragments arranged by size: 2377 1553 926 901 670 262 197 SnaBI TAC'GTA Cuts at: 493 4677 493 Size: 4184 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 2831 3247 4244 1132 Size: 1699 416 997 3774 Fragments arranged by size: 3774 1699 997 416 SspI AAT'ATT Cuts at: 5 52 4810 5 Size: 47 4758 2081 Fragments arranged by size: 4758 2081 47 StuI AGG'CCT Cuts at: 1517 3233 1517 Size: 1716 5170 StyI C'CwwG_G Cuts at: 513 1065 2234 2537 3317 4325 513 Size: 552 1169 303 780 1008 3074 Fragments arranged by size: 3074 1169 1008 780 552 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3653 3882 4913 4930 5083 5268 5713 Size: 1255 2073 229 1031 17 153 185 445 Cuts at: 5713 325 Size: 1498 Fragments arranged by size: 2073 1498 1255 1031 445 229 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3547 3649 5132 Size: 274 80 33 51 1443 1570 102 1483 Cuts at: 5132 6844 96 Size: 1712 138 Fragments arranged by size: 1712 1570 1483 1443 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6846 6878 6846 Size: 32 6854 Tth111I GACn'n_nGTC Cuts at: 1316 2477 2765 1316 Size: 1161 288 5437 Fragments arranged by size: 5437 1161 288 XbaI T'CTAG_A Cuts at: 4222 4222 Size: 6886 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3064 3247 3064 Size: 183 6703 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6886 XmaI C'CCGG_G Cuts at: 1870 4211 1870 Size: 2341 4545 XmnI GAAnn'nnTTC Cuts at: 2820 3294 5015 2820 Size: 474 1721 4691 Fragments arranged by size: 4691 1721 474 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4694 276 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI