MMP27 ORF clone pFN21AE0039

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0039

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Clone info.

Species

human

Clone Name

pFN21AE0039 Sequence (with vector) Restriction Map

Product ID

FHC10718

Flexi Clone PID

FXC10718

ORF Sequence

DNA sequence (1539 bp)
Protein sequence (513 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527320]

Gene

KIEE0039

Symbol

MMP27

Alias : MMP-27

Description

matrix metallopeptidase 27

Original Clone ID

oc00739

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1539 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 513 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8319.1 0 100.0 MMP27 CCDS8320.1 1.1e-99 48.6 MMP8 CCDS8322.1 1.1e-99 50.2 MMP1 CCDS73375.1 7.4e-98 48.7 MMP12 CCDS8324.1 7.4e-98 48.3 MMP13 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR021190 86 99 PR00138 Peptidase M10A IPR021190 131 146 PR00138 Peptidase M10A IPR021190 155 183 PR00138 Peptidase M10A IPR021190 213 238 PR00138 Peptidase M10A IPR021190 248 261 PR00138 Peptidase M10A HMMPfam IPR002477 27 86 PF01471 Peptidoglycan binding-like IPR001818 106 261 PF00413 Peptidase M10 IPR018487 285 326 PF00045 Hemopexin/matrixin IPR018487 330 370 PF00045 Hemopexin/matrixin IPR018487 376 421 PF00045 Hemopexin/matrixin HMMSmart IPR006026 102 262 SM00235 Peptidase IPR018487 285 327 SM00120 Hemopexin/matrixin IPR018487 329 371 SM00120 Hemopexin/matrixin IPR018487 376 423 SM00120 Hemopexin/matrixin IPR018487 425 465 SM00120 Hemopexin/matrixin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 4 LLLLFLFFITFSSAFPLVRMT 24 PRIMARY 21
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002415 467 120355 48.6 90.8 NP_002412 469 120353,226600,606963 50.2 90.3 NP_002417 470 601046 48.7 90.3 NP_002418 471 250400,600108,602111 48.3 90.1 XP_011541137 444 120355 50.0 84.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0039 Download

>KIEE0039  1539 bp
ATGAAGCGCCTTCTGCTTCTGTTTTTGTTCTTTATAACATTTTCTTCTGCATTTCCCTTA
GTCCGGATGACGGAAAATGAAGAAAATATGCAACTGGCTCAGGCATATCTCAACCAGTTC
TACTCTCTTGAAATAGAAGGGAATCATCTTGTTCAAAGCAAGAATAGGAGTCTCATAGAT
GACAAAATTCGGGAAATGCAAGCATTTTTTGGATTGACAGTGACTGGAAAACTGGACTCA
AACACCCTTGAGATCATGAAGACACCCAGGTGTGGGGTGCCTGATGTGGGCCAGTATGGC
TACACCCTCCCTGGGTGGAGAAAATACAACCTCACCTACAGAATAATAAACTATACTCCG
GATATGGCACGAGCTGCTGTGGATGAGGCTATCCAAGAAGGTTTAGAAGTGTGGAGCAAA
GTCACTCCACTAAAATTCACCAAGATTTCAAAGGGGATTGCAGACATCATGATTGCCTTT
AGGACTCGAGTCCATGGTCGGTGTCCTCGCTATTTTGATGGTCCCTTGGGAGTGCTTGGC
CATGCCTTTCCTCCTGGTCCGGGTCTGGGTGGTGACACTCATTTTGATGAGGATGAAAAC
TGGACCAAGGATGGAGCAGGATTCAACTTGTTTCTTGTGGCTGCTCATGAATTTGGTCAT
GCACTGGGGCTCTCTCACTCCAATGATCAAACAGCCTTGATGTTCCCAAATTATGTCTCC
CTGGATCCCAGAAAATACCCACTTTCTCAGGATGATATCAATGGAATCCAGTCCATCTAT
GGAGGTCTGCCTAAGGAACCTGCTAAGCCAAAGGAACCCACTATACCCCATGCCTGTGAC
CCTGACTTGACTTTTGACGCTATCACAACTTTCCGCAGAGAAGTAATGTTCTTTAAAGGC
AGGCACCTATGGAGGATCTATTATGATATCACGGATGTTGAGTTTGAATTAATTGCTTCA
TTCTGGCCATCTCTGCCAGCTGATCTGCAAGCTGCATACGAGAACCCCAGAGATAAGATT
CTGGTTTTTAAAGATGAAAACTTCTGGATGATCAGAGGATATGCTGTCTTGCCAGATTAT
CCCAAATCCATCCATACATTAGGTTTTCCAGGACGTGTGAAGAAAATAGATGCAGCCGTC
TGTGATAAGACCACAAGAAAAACCTACTTCTTTGTGGGCATTTGGTGCTGGAGGTTTGAT
GAAATGACCCAAACCATGGACAAAGGGTTCCCGCAGAGAGTGGTAAAACACTTTCCTGGA
ATCAGTATCCGTGTTGATGCTGCTTTCCAGTACAAAGGATTCTTCTTTTTCAGCCGTGGA
TCAAAGCAATTTGAATACGACATTAAGACAAAGAATATTACCCGAATCATGAGAACTAAT
ACTTGGTTTCAATGCAAAGAACCAAAGAACTCCTCATTTGGTTTTGATATCAACAAGGAA
AAAGCACATTCAGGAGGCATAAAGATATTGTATCATAAGAGTTTAAGCTTGTTTATTTTT
GGTATTGTTCATTTGCTGAAAAACACTTCTATTTATCAA

ORF Protein

Cloned ORF protein sequence for pFN21AE0039 Download

>KIEE0039  513 aa
MKRLLLLFLFFITFSSAFPLVRMTENEENMQLAQAYLNQFYSLEIEGNHLVQSKNRSLID
DKIREMQAFFGLTVTGKLDSNTLEIMKTPRCGVPDVGQYGYTLPGWRKYNLTYRIINYTP
DMARAAVDEAIQEGLEVWSKVTPLKFTKISKGIADIMIAFRTRVHGRCPRYFDGPLGVLG
HAFPPGPGLGGDTHFDEDENWTKDGAGFNLFLVAAHEFGHALGLSHSNDQTALMFPNYVS
LDPRKYPLSQDDINGIQSIYGGLPKEPAKPKEPTIPHACDPDLTFDAITTFRREVMFFKG
RHLWRIYYDITDVEFELIASFWPSLPADLQAAYENPRDKILVFKDENFWMIRGYAVLPDY
PKSIHTLGFPGRVKKIDAAVCDKTTRKTYFFVGIWCWRFDEMTQTMDKGFPQRVVKHFPG
ISIRVDAAFQYKGFFFFSRGSKQFEYDIKTKNITRIMRTNTWFQCKEPKNSSFGFDINKE
KAHSGGIKILYHKSLSLFIFGIVHLLKNTSIYQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0039 Download

>pFN21AE0039  6244 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGCGCCTTCTGCTTCTGTTTTTGTTCTTTAT
AACATTTTCTTCTGCATTTCCCTTAGTCCGGATGACGGAAAATGAAGAAAATATGCAACT
GGCTCAGGCATATCTCAACCAGTTCTACTCTCTTGAAATAGAAGGGAATCATCTTGTTCA
AAGCAAGAATAGGAGTCTCATAGATGACAAAATTCGGGAAATGCAAGCATTTTTTGGATT
GACAGTGACTGGAAAACTGGACTCAAACACCCTTGAGATCATGAAGACACCCAGGTGTGG
GGTGCCTGATGTGGGCCAGTATGGCTACACCCTCCCTGGGTGGAGAAAATACAACCTCAC
CTACAGAATAATAAACTATACTCCGGATATGGCACGAGCTGCTGTGGATGAGGCTATCCA
AGAAGGTTTAGAAGTGTGGAGCAAAGTCACTCCACTAAAATTCACCAAGATTTCAAAGGG
GATTGCAGACATCATGATTGCCTTTAGGACTCGAGTCCATGGTCGGTGTCCTCGCTATTT
TGATGGTCCCTTGGGAGTGCTTGGCCATGCCTTTCCTCCTGGTCCGGGTCTGGGTGGTGA
CACTCATTTTGATGAGGATGAAAACTGGACCAAGGATGGAGCAGGATTCAACTTGTTTCT
TGTGGCTGCTCATGAATTTGGTCATGCACTGGGGCTCTCTCACTCCAATGATCAAACAGC
CTTGATGTTCCCAAATTATGTCTCCCTGGATCCCAGAAAATACCCACTTTCTCAGGATGA
TATCAATGGAATCCAGTCCATCTATGGAGGTCTGCCTAAGGAACCTGCTAAGCCAAAGGA
ACCCACTATACCCCATGCCTGTGACCCTGACTTGACTTTTGACGCTATCACAACTTTCCG
CAGAGAAGTAATGTTCTTTAAAGGCAGGCACCTATGGAGGATCTATTATGATATCACGGA
TGTTGAGTTTGAATTAATTGCTTCATTCTGGCCATCTCTGCCAGCTGATCTGCAAGCTGC
ATACGAGAACCCCAGAGATAAGATTCTGGTTTTTAAAGATGAAAACTTCTGGATGATCAG
AGGATATGCTGTCTTGCCAGATTATCCCAAATCCATCCATACATTAGGTTTTCCAGGACG
TGTGAAGAAAATAGATGCAGCCGTCTGTGATAAGACCACAAGAAAAACCTACTTCTTTGT
GGGCATTTGGTGCTGGAGGTTTGATGAAATGACCCAAACCATGGACAAAGGGTTCCCGCA
GAGAGTGGTAAAACACTTTCCTGGAATCAGTATCCGTGTTGATGCTGCTTTCCAGTACAA
AGGATTCTTCTTTTTCAGCCGTGGATCAAAGCAATTTGAATACGACATTAAGACAAAGAA
TATTACCCGAATCATGAGAACTAATACTTGGTTTCAATGCAAAGAACCAAAGAACTCCTC
ATTTGGTTTTGATATCAACAAGGAAAAAGCACATTCAGGAGGCATAAAGATATTGTATCA
TAAGAGTTTAAGCTTGTTTATTTTTGGTATTGTTCATTTGCTGAAAAACACTTCTATTTA
TCAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT