(Circular) (Six-base) MAPSORT of: pFN21AE0039.seq Check: 8686 from: 1 to: 6244 pFN21AE0039 6244 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4054 278 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3587 1937 Size: 1650 4594 Acc65I G'GTAC_C Cuts at: 3565 3565 Size: 6244 AclI AA'CG_TT Cuts at: 1563 4371 4744 1563 Size: 2808 373 3063 Fragments arranged by size: 3063 2808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4307 5691 5959 1632 Size: 2675 1384 268 1917 Fragments arranged by size: 2675 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6047 AflIII A'CryG_T Cuts at: 1203 3118 5143 6120 1203 Size: 1915 2025 977 1327 Fragments arranged by size: 2025 1915 1327 977 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 4973 4973 Size: 6244 AleI CACnn'nnGTG Cuts at: 1166 2272 1166 Size: 1106 5138 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6244 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3215 3247 3215 Size: 32 6212 AlwNI CAG_nnn'CTG Cuts at: 2228 3987 5559 2228 Size: 1759 1572 2913 Fragments arranged by size: 2913 1759 1572 ApaLI G'TGCA_C Cuts at: 4301 5457 4301 Size: 1156 5088 ApoI r'AATT_y Cuts at: 1493 2190 2438 2654 3553 3779 3931 6040 1493 Size: 697 248 216 899 226 152 2109 1697 Fragments arranged by size: 2109 1697 899 697 248 226 216 152 AseI AT'TA_AT Cuts at: 160 1032 2954 4798 160 Size: 872 1922 1844 1606 Fragments arranged by size: 1922 1844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 2490 3569 1870 Size: 73 547 1079 4545 Fragments arranged by size: 4545 1079 547 73 BamHI G'GATC_C Cuts at: 2728 3574 3941 2728 Size: 846 367 5031 Fragments arranged by size: 5031 846 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2281 2907 3565 5020 618 Size: 325 217 627 494 626 658 1455 1842 Fragments arranged by size: 1842 1455 658 627 626 494 325 217 BanII G_rGCy'C Cuts at: 1391 2676 3563 1391 Size: 1285 887 4072 Fragments arranged by size: 4072 1285 887 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2270 928 Size: 971 371 4902 Fragments arranged by size: 4902 971 371 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4435 4469 1903 Size: 34 2498 34 3678 Fragments arranged by size: 3678 2498 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3281 4136 5346 3281 Size: 855 1210 4179 Fragments arranged by size: 4179 1210 855 BclI T'GATC_A Cuts at: 1548 2689 3054 1548 Size: 1141 365 4738 Fragments arranged by size: 4738 1141 365 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2301 4855 136 Size: 107 122 71 631 605 629 2554 1525 Fragments arranged by size: 2554 1525 631 629 605 122 107 71 BglII A'GATC_T Cuts at: 6239 6239 Size: 6244 BlpI GC'TnA_GC Cuts at: 2808 3661 2808 Size: 853 5391 Bme1580I G_kGCm'C Cuts at: 1790 4305 5461 1790 Size: 2515 1156 2573 Fragments arranged by size: 2573 2515 1156 BmgBI CAC'GTC Cuts at: 1318 3119 1318 Size: 1801 4443 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2678 3975 4933 Size: 416 53 83 399 24 1261 1297 958 Cuts at: 4933 442 Size: 1753 Fragments arranged by size: 1753 1297 1261 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3214 4904 1239 Size: 1975 1690 2579 Fragments arranged by size: 2579 1975 1690 Bpu10I CC'TnA_GC Cuts at: 2103 2103 Size: 6244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2273 4369 5234 5532 2273 Size: 2096 865 298 2985 Fragments arranged by size: 2985 2096 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4907 882 Size: 617 3408 2219 Fragments arranged by size: 3408 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4035 493 Size: 711 2831 2702 Fragments arranged by size: 2831 2702 711 BsaBI GATnn'nnATC Cuts at: 6238 6238 Size: 6244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4051 4433 5068 275 Size: 53 83 186 936 2518 382 635 1451 Fragments arranged by size: 2518 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2067 2362 4677 5075 5349 5496 5907 2067 Size: 295 2315 398 274 147 411 2404 Fragments arranged by size: 2404 2315 411 398 295 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6019 Fragments arranged by size: 6019 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3406 1186 Size: 2220 4024 BseYI C'CCAG_C Cuts at: 5447 5943 5447 Size: 496 5748 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5671 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3694 4455 5483 1540 Size: 275 186 1693 761 1028 2301 Fragments arranged by size: 2301 1693 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4305 4390 5461 4305 Size: 85 1071 5088 Fragments arranged by size: 5088 1071 85 BsmI GAATG_Cn' Cuts at: 3755 3848 3755 Size: 93 6151 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2676 3563 4305 4390 5461 1391 Size: 399 886 887 742 85 1071 2174 Fragments arranged by size: 2174 1071 887 886 742 399 85 BspEI T'CCGG_A Cuts at: 2067 2362 5907 2067 Size: 295 3545 2404 Fragments arranged by size: 3545 2404 295 BspHI T'CATG_A Cuts at: 2259 2472 2650 3372 4133 5863 2259 Size: 213 178 722 761 1730 2640 Fragments arranged by size: 2640 1730 761 722 213 178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2812 3599 844 Size: 1968 787 3489 Fragments arranged by size: 3489 1968 787 BsrBI CCG'CTC Cuts at: 1394 1823 4131 1394 Size: 429 2308 3507 Fragments arranged by size: 3507 2308 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4739 4913 65 Size: 1183 3491 174 1396 Fragments arranged by size: 3491 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4888 5075 1700 Size: 110 3078 187 2869 Fragments arranged by size: 3078 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6244 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6244 BssSI C'ACGA_G Cuts at: 2373 4304 5316 2373 Size: 1931 1012 3301 Fragments arranged by size: 3301 1931 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2378 3189 794 Size: 1584 811 3849 Fragments arranged by size: 3849 1584 811 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6244 BstYI r'GATC_y Cuts at: 1972 2728 2919 3574 3941 4326 4343 5795 5881 Size: 756 191 655 367 385 17 1452 86 Cuts at: 5881 5893 6239 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 756 655 385 367 346 191 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2796 2796 Size: 6244 BtgI C'CryG_G Cuts at: 513 1065 2497 3219 3319 513 Size: 552 1432 722 100 3438 Fragments arranged by size: 3438 1432 722 552 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5505 Fragments arranged by size: 5505 683 56 BtsI GCAGTG_nn' Cuts at: 3760 4554 4574 3760 Size: 794 20 5430 Fragments arranged by size: 5430 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6244 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 2899 3034 3548 2899 Size: 135 514 5595 Fragments arranged by size: 5595 514 135 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5251 809 Size: 507 3935 1802 Fragments arranged by size: 3935 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2542 2969 3691 Size: 54 1425 50 275 18 712 427 722 Cuts at: 3691 4580 6209 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 722 712 427 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3691 1537 Size: 275 1879 4090 Fragments arranged by size: 4090 1879 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4174 1353 Size: 2821 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4831 5205 5351 387 Size: 1064 138 3242 374 146 1280 Fragments arranged by size: 3242 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3214 4307 4904 5691 5959 1239 Size: 393 1582 1093 597 787 268 1524 Fragments arranged by size: 1582 1524 1093 787 597 393 268 EcoRI G'AATT_C Cuts at: 3553 3931 3553 Size: 378 5866 EcoRV GAT'ATC Cuts at: 2761 2932 3433 2761 Size: 171 501 5572 Fragments arranged by size: 5572 501 171 FspI TGC'GCA Cuts at: 4750 4750 Size: 6244 HaeII r_GCGC'y Cuts at: 1678 2014 5391 1678 Size: 336 3377 2531 Fragments arranged by size: 3377 2531 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3054 3086 3460 3492 4885 Size: 32 135 32 1482 32 374 32 1393 Cuts at: 4885 4917 4959 4991 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1482 1393 374 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3588 3834 669 Size: 988 281 1650 246 3079 Fragments arranged by size: 3079 1650 988 281 246 HindIII A'AGCT_T Cuts at: 748 3490 748 Size: 2742 3502 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KpnI G_GTAC'C Cuts at: 3569 3569 Size: 6244 MfeI C'AATT_G Cuts at: 3843 3843 Size: 6244 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5358 5542 1305 Size: 4053 184 2007 Fragments arranged by size: 4053 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2544 2971 10 Size: 54 1425 343 712 427 3283 Fragments arranged by size: 3283 1425 712 427 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2272 2589 2685 2838 4202 4561 4720 Size: 648 1106 317 96 153 1364 359 159 Cuts at: 4720 518 Size: 2042 Fragments arranged by size: 2042 1364 1106 648 359 317 159 153 96 MspA1I CmG'CkG Cuts at: 1606 2984 3660 4337 5485 5730 1606 Size: 1378 676 677 1148 245 2120 Fragments arranged by size: 2120 1378 1148 677 676 245 NcoI C'CATG_G Cuts at: 513 1065 2497 3219 513 Size: 552 1432 722 3538 Fragments arranged by size: 3538 1432 722 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 3691 3691 Size: 6244 NspI r_CATG'y Cuts at: 1132 1229 3602 5147 1132 Size: 97 2373 1545 2229 Fragments arranged by size: 2373 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2315 1348 Size: 967 5277 PciI A'CATG_T Cuts at: 5143 5143 Size: 6244 PflMI CCAn_nnn'nTGG Cuts at: 1108 3220 1108 Size: 2112 4132 PfoI T'CCnGG_A Cuts at: 3112 3259 3112 Size: 147 6097 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4313 4345 5826 5858 4313 Size: 32 1481 32 4699 Fragments arranged by size: 4699 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2492 1613 Size: 879 5365 PsiI TTA'TAA Cuts at: 2039 3814 2039 Size: 1775 4469 PspXI vC'TCGA_Gb Cuts at: 2490 2490 Size: 6244 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2811 2843 2811 Size: 32 6212 PstI C_TGCA'G Cuts at: 830 3596 830 Size: 2766 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 PvuII CAG'CTG Cuts at: 2984 2984 Size: 6244 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SbfI CC_TGCA'GG Cuts at: 3596 3596 Size: 6244 ScaI AGT'ACT Cuts at: 4492 6204 4492 Size: 1712 4532 SfcI C'TryA_G Cuts at: 826 1045 2341 3592 4727 5408 5599 826 Size: 219 1296 1251 1135 681 191 1471 Fragments arranged by size: 1471 1296 1251 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 3571 1872 Size: 1699 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 2490 4348 5249 5511 820 Size: 197 926 309 238 1858 901 262 1553 Fragments arranged by size: 1858 1553 926 901 309 262 238 197 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 3361 4168 5 Size: 47 3309 807 2081 Fragments arranged by size: 3309 2081 807 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6244 StyI C'CwwG_G Cuts at: 513 1065 2497 2529 2610 3219 3683 513 Size: 552 1432 32 81 609 464 3074 Fragments arranged by size: 3074 1432 609 552 464 81 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2284 2307 2561 4271 4288 4441 4626 Size: 1255 704 23 254 1710 17 153 185 Cuts at: 4626 5071 325 Size: 445 1498 Fragments arranged by size: 1710 1498 1255 704 445 254 185 153 23 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3294 4490 6202 Size: 274 80 33 51 1443 1317 1196 1712 Cuts at: 6202 96 Size: 138 Fragments arranged by size: 1712 1443 1317 1196 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2208 2240 6204 6236 2208 Size: 32 3964 32 2216 Fragments arranged by size: 3964 2216 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6244 XbaI T'CTAG_A Cuts at: 3580 3580 Size: 6244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2780 2780 Size: 6244 XhoI C'TCGA_G Cuts at: 1943 2490 1943 Size: 547 5697 XmaI C'CCGG_G Cuts at: 1870 3569 1870 Size: 1699 4545 XmnI GAAnn'nnTTC Cuts at: 4373 4373 Size: 6244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4052 276 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI