DPY19L4 ORF clone pFN21AE0013

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0013

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Clone info.

Species

human

Clone Name

pFN21AE0013 Sequence (with vector) Restriction Map

Product ID

FHC10782

Flexi Clone PID

FXC10782

ORF Sequence

DNA sequence (2169 bp)
Protein sequence (723 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587515]

Gene

KIEE0013

Symbol

DPY19L4

Description

dpy-19-like 4 (C. elegans)

Original Clone ID

oc00713

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2169 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 723 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34924.1 0 99.9 DPY19L4 CCDS12422.1 1.8e-134 43.4 DPY19L3 CCDS43567.1 2.9e-16 25.9 DPY19L1 CCDS31851.1 1.8e-14 26.5 DPY19L2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018732 59 715 PF10034 Dpy-19
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 161 VYFYIGIVFGLQGIYVTALFVTS 183 PRIMARY 23 2 188 GTWLAGMLTVAWFVINRVDTTRI 210 SECONDARY 23 3 267 HYLLFLQAISLFLLDTFSVEQSD 289 SECONDARY 23 4 303 FLGYLLQFENPALLVSPLLSLVA 325 PRIMARY 23 5 345 KIIKVINFYLVCTLTITLNIIMK 367 PRIMARY 23 6 420 SLLPFYILVLIICFLSMLQVIF 441 PRIMARY 22 7 474 ILLGSLAMVIEGLKYIWIPYVCM 496 PRIMARY 23 8 520 VHPILLALILSMAVPTIIGLSLW 542 PRIMARY 23 9 573 RQAPVAAVFAGSPQLMGAIKLCT 595 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_861452 723 613895 99.9 100.0 XP_005250951 797 613895 99.9 99.4 XP_016868843 618 613895 100.0 85.5 XP_005250952 618 613895 100.0 85.5 XP_016868841 651 613895 89.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0013 Download

>KIEE0013  2169 bp
ATGGCGGACGAAGAAGGACCACCTGTAGAGCTGCGCCAAAGAAAAAAGCCAAAGTCTTCA
GAAAATAAGGAATCTGCCAAAGAAGAGAAAATCAGTGACATTCCAATTCCTGAAAGAGCT
CCAAAACATGTATTATTTCAACGCTTTGCAAAGATTTTCATTGGCTGTCTTGCAGCGGTT
ACTAGTGGTATGATGTATGCTCTCTACTTATCAGCATACCATGAACGGAAATTCTGGTTT
TCCAACAGGCAGGAGCTTGAACGGGAAATCACGTTTCAGGGTGACAGTGCCATTTATTAC
TCCTATTATAAAGATATGTTAAAGGCACCTTCATTTGAAAGAGGTGTTTACGAACTGACA
CACAATAACAAAACTGTATCTCTGAAGACTATAAATGCAGTGCAGCAAATGTCTCTGTAT
CCGGAACTTATTGCTAGCATTTTATATCAAGCCACTGGTAGCAATGAGATTATTGAGCCA
GTGTATTTCTATATTGGCATTGTTTTTGGATTGCAAGGAATATATGTTACTGCTTTATTT
GTTACAAGTTGGCTTATGAGTGGAACATGGCTAGCAGGAATGCTTACTGTTGCGTGGTTC
GTTATTAACAGGGTAGATACAACAAGAATTGAATACTCCATTCCTTTAAGAGAAAACTGG
GCACTACCATATTTTGCATGCCAAATTGCTGCACTTACAGGCTATTTAAAAAGCAACTTA
AATACTTATGGAGAGAGGTTTTGCTACTTGTTGATGAGTGCTTCAACTTACACATTTATG
ATGATGTGGGAGTATAGCCACTATCTCCTGTTTCTTCAAGCAATATCTCTATTCCTGCTA
GATACCTTTTCAGTGGAGCAAAGTGACAAGGTTTATGAAGTTTATAAAATCTACATATTT
TCCCTCTTTCTGGGATATTTACTACAGTTTGAGAATCCAGCTTTGTTGGTATCTCCTTTA
TTAAGTTTAGTAGCAGCCTTAATGCTTGCTAAGTGCCTTCAGCTGAATGTGAAGAAAGGA
AGTTTTGTAGCTAAAATAATAAAAGTGATTAATTTTTACTTGGTGTGTACTCTGACAATA
ACATTGAATATTATAATGAAGATGTTTGTCCCACACAAAGAAAATGGGCACATGCTGAAA
TTCCTTGAAGTAAAATTTGGACTAAATATGACCAAGAATTTTACAATGAATTGGCTCCTC
TGTCAAGAATCCCTGCAGGCACCATCTCAAGATTTTTTTCTGCGATTGACACAGTCTTCT
TTATTACCTTTCTACATTCTAGTGTTAATTATTTGTTTTCTTTCTATGTTGCAAGTTATT
TTTAGGAGGATTAATGGTAAGTCCCTGAAGGAAACTGTTACTCTTGAAGATGGACGAATT
GGAGAAAGACCAGAAATAATTTATCACGTAATTCACACTATTTTATTGGGTTCTCTTGCA
ATGGTTATAGAAGGCTTGAAGTACATCTGGATTCCTTATGTGTGCATGTTAGCAGCATTT
GGTGTATGTTCTCCCGAACTTTGGATGACACTTTTCAAGTGGCTTCGATTAAGAACTGTA
CACCCAATATTGTTGGCTCTTATTCTGAGCATGGCCGTGCCTACTATAATAGGTCTCAGC
TTATGGAAAGAGTTTTTTCCCAGATTAATGACAGAATTAATGGAACTACAGGAATTCTAT
GACCCAGATACAGTGGAACTTATGACCTGGATAAAAAGGCAAGCTCCAGTTGCAGCTGTG
TTTGCAGGGAGTCCACAGTTAATGGGTGCGATTAAATTATGCACTGGATGGATGGTGACA
AGTTTGCCTCTTTACAATGATGATGATCTTCTCAAGAGAAATGAAAATATCTACCAAATC
TATTCAAAGCGATCTGCTGAGGATATTTATAAAATACTGACATCTTACAAAGCTAATTAC
CTAATTGTAGAGGATGCTATCTGCAATGAGGTGGGACCCATGAGAGGCTGTAGGGTTAAA
GATTTATTAGACATTGCAAATGGCCACATGGTTTGTGAAGAAGGTGACAAGCTAACCTAC
TCAAAATATGGGCGATTTTGTCATGAGGTCAAAATTAACTATTCTCCATATGTGAATTAT
TTCACTAGAGTATACTGGAACAGATCCTACTTTGTATATAAAATCAACACTGTGATATCC
TTCCAGTCT

ORF Protein

Cloned ORF protein sequence for pFN21AE0013 Download

>KIEE0013  723 aa
MADEEGPPVELRQRKKPKSSENKESAKEEKISDIPIPERAPKHVLFQRFAKIFIGCLAAV
TSGMMYALYLSAYHERKFWFSNRQELEREITFQGDSAIYYSYYKDMLKAPSFERGVYELT
HNNKTVSLKTINAVQQMSLYPELIASILYQATGSNEIIEPVYFYIGIVFGLQGIYVTALF
VTSWLMSGTWLAGMLTVAWFVINRVDTTRIEYSIPLRENWALPYFACQIAALTGYLKSNL
NTYGERFCYLLMSASTYTFMMMWEYSHYLLFLQAISLFLLDTFSVEQSDKVYEVYKIYIF
SLFLGYLLQFENPALLVSPLLSLVAALMLAKCLQLNVKKGSFVAKIIKVINFYLVCTLTI
TLNIIMKMFVPHKENGHMLKFLEVKFGLNMTKNFTMNWLLCQESLQAPSQDFFLRLTQSS
LLPFYILVLIICFLSMLQVIFRRINGKSLKETVTLEDGRIGERPEIIYHVIHTILLGSLA
MVIEGLKYIWIPYVCMLAAFGVCSPELWMTLFKWLRLRTVHPILLALILSMAVPTIIGLS
LWKEFFPRLMTELMELQEFYDPDTVELMTWIKRQAPVAAVFAGSPQLMGAIKLCTGWMVT
SLPLYNDDDLLKRNENIYQIYSKRSAEDIYKILTSYKANYLIVEDAICNEVGPMRGCRVK
DLLDIANGHMVCEEGDKLTYSKYGRFCHEVKINYSPYVNYFTRVYWNRSYFVYKINTVIS
FQS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0013 Download

>pFN21AE0013  6874 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGACGAAGAAGGACCACCTGTAGAGCTGCG
CCAAAGAAAAAAGCCAAAGTCTTCAGAAAATAAGGAATCTGCCAAAGAAGAGAAAATCAG
TGACATTCCAATTCCTGAAAGAGCTCCAAAACATGTATTATTTCAACGCTTTGCAAAGAT
TTTCATTGGCTGTCTTGCAGCGGTTACTAGTGGTATGATGTATGCTCTCTACTTATCAGC
ATACCATGAACGGAAATTCTGGTTTTCCAACAGGCAGGAGCTTGAACGGGAAATCACGTT
TCAGGGTGACAGTGCCATTTATTACTCCTATTATAAAGATATGTTAAAGGCACCTTCATT
TGAAAGAGGTGTTTACGAACTGACACACAATAACAAAACTGTATCTCTGAAGACTATAAA
TGCAGTGCAGCAAATGTCTCTGTATCCGGAACTTATTGCTAGCATTTTATATCAAGCCAC
TGGTAGCAATGAGATTATTGAGCCAGTGTATTTCTATATTGGCATTGTTTTTGGATTGCA
AGGAATATATGTTACTGCTTTATTTGTTACAAGTTGGCTTATGAGTGGAACATGGCTAGC
AGGAATGCTTACTGTTGCGTGGTTCGTTATTAACAGGGTAGATACAACAAGAATTGAATA
CTCCATTCCTTTAAGAGAAAACTGGGCACTACCATATTTTGCATGCCAAATTGCTGCACT
TACAGGCTATTTAAAAAGCAACTTAAATACTTATGGAGAGAGGTTTTGCTACTTGTTGAT
GAGTGCTTCAACTTACACATTTATGATGATGTGGGAGTATAGCCACTATCTCCTGTTTCT
TCAAGCAATATCTCTATTCCTGCTAGATACCTTTTCAGTGGAGCAAAGTGACAAGGTTTA
TGAAGTTTATAAAATCTACATATTTTCCCTCTTTCTGGGATATTTACTACAGTTTGAGAA
TCCAGCTTTGTTGGTATCTCCTTTATTAAGTTTAGTAGCAGCCTTAATGCTTGCTAAGTG
CCTTCAGCTGAATGTGAAGAAAGGAAGTTTTGTAGCTAAAATAATAAAAGTGATTAATTT
TTACTTGGTGTGTACTCTGACAATAACATTGAATATTATAATGAAGATGTTTGTCCCACA
CAAAGAAAATGGGCACATGCTGAAATTCCTTGAAGTAAAATTTGGACTAAATATGACCAA
GAATTTTACAATGAATTGGCTCCTCTGTCAAGAATCCCTGCAGGCACCATCTCAAGATTT
TTTTCTGCGATTGACACAGTCTTCTTTATTACCTTTCTACATTCTAGTGTTAATTATTTG
TTTTCTTTCTATGTTGCAAGTTATTTTTAGGAGGATTAATGGTAAGTCCCTGAAGGAAAC
TGTTACTCTTGAAGATGGACGAATTGGAGAAAGACCAGAAATAATTTATCACGTAATTCA
CACTATTTTATTGGGTTCTCTTGCAATGGTTATAGAAGGCTTGAAGTACATCTGGATTCC
TTATGTGTGCATGTTAGCAGCATTTGGTGTATGTTCTCCCGAACTTTGGATGACACTTTT
CAAGTGGCTTCGATTAAGAACTGTACACCCAATATTGTTGGCTCTTATTCTGAGCATGGC
CGTGCCTACTATAATAGGTCTCAGCTTATGGAAAGAGTTTTTTCCCAGATTAATGACAGA
ATTAATGGAACTACAGGAATTCTATGACCCAGATACAGTGGAACTTATGACCTGGATAAA
AAGGCAAGCTCCAGTTGCAGCTGTGTTTGCAGGGAGTCCACAGTTAATGGGTGCGATTAA
ATTATGCACTGGATGGATGGTGACAAGTTTGCCTCTTTACAATGATGATGATCTTCTCAA
GAGAAATGAAAATATCTACCAAATCTATTCAAAGCGATCTGCTGAGGATATTTATAAAAT
ACTGACATCTTACAAAGCTAATTACCTAATTGTAGAGGATGCTATCTGCAATGAGGTGGG
ACCCATGAGAGGCTGTAGGGTTAAAGATTTATTAGACATTGCAAATGGCCACATGGTTTG
TGAAGAAGGTGACAAGCTAACCTACTCAAAATATGGGCGATTTTGTCATGAGGTCAAAAT
TAACTATTCTCCATATGTGAATTATTTCACTAGAGTATACTGGAACAGATCCTACTTTGT
ATATAAAATCAACACTGTGATATCCTTCCAGTCTGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT