(Circular) (Six-base) MAPSORT of: pFN21AE0013.seq Check: 1395 from: 1 to: 6874 pFN21AE0013 6874 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4684 278 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4116 4217 1937 Size: 2179 101 4594 Fragments arranged by size: 4594 2179 101 Acc65I G'GTAC_C Cuts at: 4195 4195 Size: 6874 AclI AA'CG_TT Cuts at: 1563 5001 5374 1563 Size: 3438 373 3063 Fragments arranged by size: 3438 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2046 2408 2987 3371 4937 6321 6589 1632 Size: 414 362 579 384 1566 1384 268 1917 Fragments arranged by size: 1917 1566 1384 579 414 384 362 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6677 AflIII A'CryG_T Cuts at: 1203 2131 5773 6750 1203 Size: 928 3642 977 1327 Fragments arranged by size: 3642 1327 977 928 AgeI A'CCGG_T Cuts at: 5705 5705 Size: 6874 AhdI GACnn_n'nnGTC Cuts at: 5603 5603 Size: 6874 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6874 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3617 1184 Size: 2433 4441 AlwNI CAG_nnn'CTG Cuts at: 4617 6189 4617 Size: 1572 5302 ApaLI G'TGCA_C Cuts at: 4931 6087 4931 Size: 1156 5718 ApoI r'AATT_y Cuts at: 1493 2234 3143 3158 3181 3677 4183 4409 4561 Size: 741 909 15 23 496 506 226 152 Cuts at: 4561 6670 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 909 741 506 496 226 152 23 15 AseI AT'TA_AT Cuts at: 160 1032 3054 3336 3650 3662 5428 160 Size: 872 2022 282 314 12 1766 1606 Fragments arranged by size: 2022 1766 1606 872 314 282 12 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 AvaI C'yCGr_G Cuts at: 1870 1943 4199 1870 Size: 73 2256 4545 Fragments arranged by size: 4545 2256 73 BamHI G'GATC_C Cuts at: 4204 4571 4204 Size: 367 6507 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2329 3223 4195 5650 618 Size: 325 217 627 542 894 972 1455 1842 Fragments arranged by size: 1842 1455 972 894 627 542 325 217 BanII G_rGCy'C Cuts at: 1391 2125 4193 1391 Size: 734 2068 4072 Fragments arranged by size: 4072 2068 734 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2052 2396 3252 928 Size: 971 153 344 856 4550 Fragments arranged by size: 4550 971 856 344 153 BbvCI CC'TCA_GC Cuts at: 3882 3882 Size: 6874 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5065 5099 1903 Size: 34 3128 34 3678 Fragments arranged by size: 3678 3128 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2433 4766 5976 2433 Size: 2333 1210 3331 Fragments arranged by size: 3331 2333 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6874 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5485 136 Size: 107 122 71 631 605 3813 1525 Fragments arranged by size: 3813 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6869 6869 Size: 6874 BlpI GC'TnA_GC Cuts at: 4291 4291 Size: 6874 Bme1580I G_kGCm'C Cuts at: 1790 2668 3135 4935 6091 1790 Size: 878 467 1800 1156 2573 Fragments arranged by size: 2573 1800 1156 878 467 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6874 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2671 4605 5563 Size: 416 53 83 399 24 1254 1934 958 Cuts at: 5563 442 Size: 1753 Fragments arranged by size: 1934 1753 1254 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2442 2579 1056 Size: 1386 137 5351 Fragments arranged by size: 5351 1386 137 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3714 5534 1239 Size: 2475 1820 2579 Fragments arranged by size: 2579 2475 1820 Bpu10I CC'TnA_GC Cuts at: 3882 3882 Size: 6874 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3216 3821 4999 5864 6162 3216 Size: 605 1178 865 298 3928 Fragments arranged by size: 3928 1178 865 605 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3623 5537 882 Size: 617 2124 1914 2219 Fragments arranged by size: 2219 2124 1914 617 BsaAI yAC'GTr Cuts at: 493 1204 3412 4665 493 Size: 711 2208 1253 2702 Fragments arranged by size: 2702 2208 1253 711 BsaBI GATnn'nnATC Cuts at: 3828 6868 3828 Size: 3040 3834 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4681 5063 5698 275 Size: 53 83 186 936 3148 382 635 1451 Fragments arranged by size: 3148 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2425 5307 5705 5979 6126 6537 2425 Size: 2882 398 274 147 411 2762 Fragments arranged by size: 2882 2762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6649 Fragments arranged by size: 6649 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3191 1186 Size: 2005 4869 BseYI C'CCAG_C Cuts at: 6077 6573 6077 Size: 496 6378 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2426 2679 1163 Size: 573 690 253 5358 Fragments arranged by size: 5358 690 573 253 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4324 5085 6113 1540 Size: 275 186 2323 761 1028 2301 Fragments arranged by size: 2323 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2125 4935 5020 6091 2125 Size: 2810 85 1071 2908 Fragments arranged by size: 2908 2810 1071 85 BsmI GAATG_Cn' Cuts at: 2589 4385 4478 2589 Size: 1796 93 4985 Fragments arranged by size: 4985 1796 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2125 2668 3135 4193 4935 5020 6091 Size: 399 335 543 467 1058 742 85 1071 Cuts at: 6091 1391 Size: 2174 Fragments arranged by size: 2174 1071 1058 742 543 467 399 335 85 BspEI T'CCGG_A Cuts at: 2425 6537 2425 Size: 4112 2762 BspHI T'CATG_A Cuts at: 4066 4763 6493 4066 Size: 697 1730 4447 Fragments arranged by size: 4447 1730 697 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4229 844 Size: 3385 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4761 1394 Size: 429 2938 3507 Fragments arranged by size: 3507 2938 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2473 3450 3955 3996 5369 5543 65 Size: 1183 1225 977 505 41 1373 174 1396 Fragments arranged by size: 1396 1373 1225 1183 977 505 174 41 BsrFI r'CCGG_y Cuts at: 1700 1810 5518 5705 1700 Size: 110 3708 187 2869 Fragments arranged by size: 3708 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3562 96 Size: 3466 3408 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6874 BssSI C'ACGA_G Cuts at: 4934 5946 4934 Size: 1012 5862 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6874 BstBI TT'CG_AA Cuts at: 5678 5678 Size: 6874 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6874 BstYI r'GATC_y Cuts at: 1972 4127 4204 4571 4956 4973 6425 6511 6523 Size: 2155 77 367 385 17 1452 86 12 Cuts at: 6523 6869 1972 Size: 346 1977 Fragments arranged by size: 2155 1977 1452 385 367 346 86 77 17 12 BstZ17I GTA'TAC Cuts at: 4117 4117 Size: 6874 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5383 Fragments arranged by size: 5383 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6135 Fragments arranged by size: 6135 683 56 BtsI GCAGTG_nn' Cuts at: 2409 4390 5184 5204 2409 Size: 1981 794 20 4079 Fragments arranged by size: 4079 1981 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6874 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3998 4033 529 Size: 35 3434 35 3370 Fragments arranged by size: 3434 3370 35 35 DraI TTT'AAA Cuts at: 2712 4178 2712 Size: 1466 5408 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5881 809 Size: 507 4565 1802 Fragments arranged by size: 4565 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3597 4006 4321 Size: 54 1425 50 275 18 1767 409 315 Cuts at: 4321 5210 6839 8 Size: 889 1629 43 Fragments arranged by size: 1767 1629 1425 889 409 315 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4321 1537 Size: 275 2509 4090 Fragments arranged by size: 4090 2509 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 4804 1353 Size: 729 2722 3423 Fragments arranged by size: 3423 2722 729 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5461 5835 5981 387 Size: 1064 138 435 3437 374 146 1280 Fragments arranged by size: 3437 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2123 2123 Size: 6874 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2408 2987 3371 3714 4937 5534 Size: 393 414 362 579 384 343 1223 597 Cuts at: 5534 6321 6589 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1223 787 597 579 414 393 384 362 343 268 EcoO109I rG'GnC_Cy Cuts at: 3959 3959 Size: 6874 EcoRI G'AATT_C Cuts at: 3677 4183 4561 3677 Size: 506 378 5990 Fragments arranged by size: 5990 506 378 EcoRV GAT'ATC Cuts at: 4161 4161 Size: 6874 FspI TGC'GCA Cuts at: 5380 5380 Size: 6874 HaeII r_GCGC'y Cuts at: 1678 6021 1678 Size: 4343 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2188 2220 5515 5547 5589 Size: 32 135 32 616 32 3295 32 42 Cuts at: 5589 5621 1373 Size: 32 2626 Fragments arranged by size: 3295 2626 616 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4218 4464 669 Size: 988 281 2280 246 3079 Fragments arranged by size: 3079 2280 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6874 HpaI GTT'AAC Cuts at: 4464 4464 Size: 6874 KpnI G_GTAC'C Cuts at: 4199 4199 Size: 6874 MfeI C'AATT_G Cuts at: 4473 4473 Size: 6874 MluI A'CGCG_T Cuts at: 6750 6750 Size: 6874 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2271 5988 6172 1305 Size: 966 3717 184 2007 Fragments arranged by size: 3717 2007 966 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4008 10 Size: 54 1425 343 2176 2876 Fragments arranged by size: 2876 2176 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2780 3284 3599 4832 5191 5350 518 Size: 648 1614 504 315 1233 359 159 2042 Fragments arranged by size: 2042 1614 1233 648 504 359 315 159 MspA1I CmG'CkG Cuts at: 1606 2180 3007 3740 4290 4967 6115 6360 1606 Size: 574 827 733 550 677 1148 245 2120 Fragments arranged by size: 2120 1148 827 733 677 574 550 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5383 Fragments arranged by size: 5383 939 552 NdeI CA'TA_TG Cuts at: 387 4093 387 Size: 3706 3168 NheI G'CTAG_C Cuts at: 1052 2438 2575 1052 Size: 1386 137 5351 Fragments arranged by size: 5351 1386 137 NotI GC'GGCC_GC Cuts at: 4321 4321 Size: 6874 NspI r_CATG'y Cuts at: 1132 1229 2135 2685 3139 3493 4232 5777 1132 Size: 97 906 550 454 354 739 1545 2229 Fragments arranged by size: 2229 1545 906 739 550 454 354 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6874 PciI A'CATG_T Cuts at: 2131 5773 2131 Size: 3642 3232 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6874 PmeI GTTT'AAAC Cuts at: 4178 4178 Size: 6874 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4943 4975 6456 6488 4943 Size: 32 1481 32 5329 Fragments arranged by size: 5329 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3959 3959 Size: 6874 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6874 PsiI TTA'TAA Cuts at: 2313 2889 3098 3894 4444 2313 Size: 576 209 796 550 4743 Fragments arranged by size: 4743 796 576 550 209 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4115 4147 4115 Size: 32 6842 PstI C_TGCA'G Cuts at: 830 3222 4226 830 Size: 2392 1004 3478 Fragments arranged by size: 3478 2392 1004 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6874 PvuII CAG'CTG Cuts at: 3007 3740 3007 Size: 733 6141 SacI G_AGCT'C Cuts at: 2125 2125 Size: 6874 SalI G'TCGA_C Cuts at: 1936 4216 1936 Size: 2280 4594 SanDI GG'GwC_CC Cuts at: 3959 3959 Size: 6874 SbfI CC_TGCA'GG Cuts at: 3222 4226 3222 Size: 1004 5870 ScaI AGT'ACT Cuts at: 5122 6834 5122 Size: 1712 5162 SfcI C'TryA_G Cuts at: 826 1045 2028 2927 3218 3671 3973 4222 5357 Size: 219 983 899 291 453 302 249 1135 Cuts at: 5357 6038 6229 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 983 899 681 453 302 291 249 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6874 SmaI CCC'GGG Cuts at: 1872 4201 1872 Size: 2329 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3231 3836 4978 5879 6141 820 Size: 197 926 1288 605 1142 901 262 1553 Fragments arranged by size: 1553 1288 1142 926 901 605 262 197 SnaBI TAC'GTA Cuts at: 493 4665 493 Size: 4172 2702 SpeI A'CTAG_T Cuts at: 152 2186 152 Size: 2034 4840 SphI G_CATG'C Cuts at: 1132 2685 4232 1132 Size: 1553 1547 3774 Fragments arranged by size: 3774 1553 1547 SspI AAT'ATT Cuts at: 5 52 3094 3573 4798 5 Size: 47 3042 479 1225 2081 Fragments arranged by size: 3042 2081 1225 479 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6874 StyI C'CwwG_G Cuts at: 513 1065 2004 4313 513 Size: 552 939 2309 3074 Fragments arranged by size: 3074 2309 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3582 3758 4901 4918 5071 5256 5701 Size: 1255 2002 176 1143 17 153 185 445 Cuts at: 5701 325 Size: 1498 Fragments arranged by size: 2002 1498 1255 1143 445 185 176 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3071 3465 3562 Size: 274 80 33 51 1443 1094 394 97 Cuts at: 3562 5120 6832 96 Size: 1558 1712 138 Fragments arranged by size: 1712 1558 1443 1094 394 274 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3783 3815 6834 6866 3783 Size: 32 3019 32 3791 Fragments arranged by size: 3791 3019 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3256 1316 Size: 1940 4934 XbaI T'CTAG_A Cuts at: 4210 4210 Size: 6874 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6874 XmaI C'CCGG_G Cuts at: 1870 4199 1870 Size: 2329 4545 XmnI GAAnn'nnTTC Cuts at: 4103 5003 4103 Size: 900 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4682 276 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI