ARHGAP22 ORF clone pFN21AB9470

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB9470

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Clone info.

Species

human

Clone Name

pFN21AB9470 Sequence (with vector) Restriction Map

Product ID

FHC01891

Flexi Clone PID

FXC01891

Original Clone PID

ORK01891

ORF Sequence

DNA sequence (1965 bp)
Protein sequence (655 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385228]

Gene

KIBB9470

Symbol

ARHGAP22

Alias : RhoGAP2, RhoGap22

Description

Rho GTPase activating protein 22

Original Clone ID

af02594

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1965 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 655 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS58080.1 1.5e-129 91.4 ARHGAP22 CCDS58079.1 3.8e-129 100.0 ARHGAP22 CCDS7227.1 4.2e-129 93.7 ARHGAP22 CCDS58081.1 4.2e-129 93.7 ARHGAP22 CCDS54363.1 3.4e-42 42.9 ARHGAP25 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 39 105 PF00169 Pleckstrin homology domain IPR000198 130 278 PF00620 Rho GTPase-activating protein domain HMMSmart IPR001849 38 121 SM00233 Pleckstrin homology domain IPR000198 127 303 SM00324 Rho GTPase-activating protein domain ProfileScan IPR001849 37 105 PS50003 Pleckstrin homology domain IPR000198 112 306 PS50238 Rho GTPase-activating protein domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016871959 655 610585 100.0 100.0 XP_011538308 661 610585 100.0 98.2 XP_005270071 624 610585 99.3 84.6 XP_011538307 624 610585 99.3 84.6 XP_011538309 637 610585 99.3 84.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9470 Download

>KIBB9470  1965 bp
ATGCTGAGCCCAAAGATCAGGCAGGCCAGGAGGGCCCGCTCCAAAAGCCTAGTGATGGGG
GAGCAGAGCCGGAGCCCTGGGCGGATGCCGTGCCCTCACAGGCTGGGCCCCGTGCTGAAG
GCGGGCTGGCTGAAGAAGCAGAGGAGCATCATGAAGAACTGGCAGCAGCGCTGGTTTGTG
CTGCGTGGGGATCAGCTTTTCTACTACAAGGACAAAGATGAGATCAAGCCCCAGGGATTT
ATTTCTCTACAAGGGACACAGGTGACTGAACTTCCTCCTGGCCCCGAGGACCCAGGGAAG
CACCTCTTTGAGATCAGCCCAGGGATCTTTGGGCAGCGCCTAGAGGAAACAGTCCACCAC
GAGCGGAAGTATGGCCCCCGCCTGGCGCCCCTGCTGGTGGAGCAGTGTGTGGACTTCATC
CGGGAGCGCGGGCTCACTGAGGAGGGGCTGTTCCGCATGCCGGGCCAGGCCAACCTGGTG
AGGGACCTGCAGGATTCCTTCGACTGTGGGGAGAAGCCACTGTTTGACAGCACAACAGAC
GTGCACACGGTGGCCTCCCTGCTGAAGCTGTACCTGCGGGAGCTCCCCGAGCCCGTGGTC
CCCTTCGCCAGGTACGAGGACTTCCTCAGCTGCGCCCAGCTGCTCACCAAGGACGAGGGG
GAGGGCACTCTGGAGTTGGCTAAACAAGTGAGCAACCTTCCTCAGGCAAATTACAACCTG
CTCAGATACATCTGCAAGTTTCTGGATGAAGTTCAGGCATACTCAAATGTCAACAAGATG
AGTGTCCAGAATCTGGCAACCGTTTTTGGACCTAACATTCTGCGGCCACAGGTAGAGGAC
CCAGTAACCATCATGGAAGGCACTTCCCTCGTCCAGCACCTGATGACCGTCCTCATCCGC
AAACACAGCCAGCTCTTCACGGCACCGGTCCCGGAAGGGCCCACCTCCCCGCGCGGGGGC
CTGCAATGCGCAGTGGGGTGGGGCTCCGAGGAGGTCACCAGGGACAGCCAAGGAGAGCCC
GGCGGCCCCGGCCTGCCCGCGCACAGGACCTCTTCCCTGGACGGGGCGGCCGTGGCGGTG
CTCTCCAGAACAGCCCCCACGGGGCCGGGGAGCCGGTGCAGCCCTGGGAAGAAGGTGCAG
ACCCTGCCCAGTTGGAAGTCCTCCTTCCGGCAGCCGAGGTCCCTATCGGGAAGCCCGAAG
GGGGGCGGCTCATCCCTGGAGGTGCCCATCATCTCCTCCGGCGGGAACTGGCTTATGAAC
GGGCTGTCCTCCCTGCGCGGACACCGCCGGGCCTCGTCGGGAGACCGGCTCAAGGACTCG
GGCTCCGTGCAGAGACTCTCCACCTACGACAATGTGCCCGCGCCGGGCCTGGTCCCCGGC
ATACCCAGCGTGGCCAGTATGGCGTGGTCCGGGGCCTCGTCCAGCGAGTCGTCGGTGGGG
GGCTCACTCAGCAGCTGCACGGCCTGCCGCGCCAGCGACTCGTCTGCCCGCAGTTCCCTG
CACACCGACTGGGCCCTGGAGCCCTCCCCGCTCCCCAGCAGCAGCGAGGACCCCAAGTCC
CTGGACCTGGACCACAGCATGGACGAGGCGGGCGCGGGTGCCAGCAACAGCGAGCCCAGC
GAGCCGGACAGCCCCACCCGGGAACACGCGCGCCGCTCCGAGGCCTTACAGGGGCTGGTC
ACTGAGCTCAGGGCCGAGCTGTGCCGCCAGCGGACTGAGTACGAGAGGAGTGTGAAAAGA
ATCGAAGAAGGGAGTGCTGACCTGAGAAAACGAATGTCCCGGTTAGAAGAAGAACTGGAC
CAGGAAAAGAAAAAATACATCATGCTGGAAATAAAGCTGCGGAACTCTGAACGGGCGCGG
GAGGATGCGGAGAGGAGGAACCAGCTGTTGCAGAGGGAAATGGAGGAGTTTTTTTCGACC
CTAGGAAGCTTGACTGTTGGGGCAAAAGGTGCCAGGGCCCCAAAG

ORF Protein

Cloned ORF protein sequence for pFN21AB9470 Download

>KIBB9470  655 aa
MLSPKIRQARRARSKSLVMGEQSRSPGRMPCPHRLGPVLKAGWLKKQRSIMKNWQQRWFV
LRGDQLFYYKDKDEIKPQGFISLQGTQVTELPPGPEDPGKHLFEISPGIFGQRLEETVHH
ERKYGPRLAPLLVEQCVDFIRERGLTEEGLFRMPGQANLVRDLQDSFDCGEKPLFDSTTD
VHTVASLLKLYLRELPEPVVPFARYEDFLSCAQLLTKDEGEGTLELAKQVSNLPQANYNL
LRYICKFLDEVQAYSNVNKMSVQNLATVFGPNILRPQVEDPVTIMEGTSLVQHLMTVLIR
KHSQLFTAPVPEGPTSPRGGLQCAVGWGSEEVTRDSQGEPGGPGLPAHRTSSLDGAAVAV
LSRTAPTGPGSRCSPGKKVQTLPSWKSSFRQPRSLSGSPKGGGSSLEVPIISSGGNWLMN
GLSSLRGHRRASSGDRLKDSGSVQRLSTYDNVPAPGLVPGIPSVASMAWSGASSSESSVG
GSLSSCTACRASDSSARSSLHTDWALEPSPLPSSSEDPKSLDLDHSMDEAGAGASNSEPS
EPDSPTREHARRSEALQGLVTELRAELCRQRTEYERSVKRIEEGSADLRKRMSRLEEELD
QEKKKYIMLEIKLRNSERAREDAERRNQLLQREMEEFFSTLGSLTVGAKGARAPK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9470 Download

>pFN21AB9470  6670 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTGAGCCCAAAGATCAGGCAGGCCAGGAGGGC
CCGCTCCAAAAGCCTAGTGATGGGGGAGCAGAGCCGGAGCCCTGGGCGGATGCCGTGCCC
TCACAGGCTGGGCCCCGTGCTGAAGGCGGGCTGGCTGAAGAAGCAGAGGAGCATCATGAA
GAACTGGCAGCAGCGCTGGTTTGTGCTGCGTGGGGATCAGCTTTTCTACTACAAGGACAA
AGATGAGATCAAGCCCCAGGGATTTATTTCTCTACAAGGGACACAGGTGACTGAACTTCC
TCCTGGCCCCGAGGACCCAGGGAAGCACCTCTTTGAGATCAGCCCAGGGATCTTTGGGCA
GCGCCTAGAGGAAACAGTCCACCACGAGCGGAAGTATGGCCCCCGCCTGGCGCCCCTGCT
GGTGGAGCAGTGTGTGGACTTCATCCGGGAGCGCGGGCTCACTGAGGAGGGGCTGTTCCG
CATGCCGGGCCAGGCCAACCTGGTGAGGGACCTGCAGGATTCCTTCGACTGTGGGGAGAA
GCCACTGTTTGACAGCACAACAGACGTGCACACGGTGGCCTCCCTGCTGAAGCTGTACCT
GCGGGAGCTCCCCGAGCCCGTGGTCCCCTTCGCCAGGTACGAGGACTTCCTCAGCTGCGC
CCAGCTGCTCACCAAGGACGAGGGGGAGGGCACTCTGGAGTTGGCTAAACAAGTGAGCAA
CCTTCCTCAGGCAAATTACAACCTGCTCAGATACATCTGCAAGTTTCTGGATGAAGTTCA
GGCATACTCAAATGTCAACAAGATGAGTGTCCAGAATCTGGCAACCGTTTTTGGACCTAA
CATTCTGCGGCCACAGGTAGAGGACCCAGTAACCATCATGGAAGGCACTTCCCTCGTCCA
GCACCTGATGACCGTCCTCATCCGCAAACACAGCCAGCTCTTCACGGCACCGGTCCCGGA
AGGGCCCACCTCCCCGCGCGGGGGCCTGCAATGCGCAGTGGGGTGGGGCTCCGAGGAGGT
CACCAGGGACAGCCAAGGAGAGCCCGGCGGCCCCGGCCTGCCCGCGCACAGGACCTCTTC
CCTGGACGGGGCGGCCGTGGCGGTGCTCTCCAGAACAGCCCCCACGGGGCCGGGGAGCCG
GTGCAGCCCTGGGAAGAAGGTGCAGACCCTGCCCAGTTGGAAGTCCTCCTTCCGGCAGCC
GAGGTCCCTATCGGGAAGCCCGAAGGGGGGCGGCTCATCCCTGGAGGTGCCCATCATCTC
CTCCGGCGGGAACTGGCTTATGAACGGGCTGTCCTCCCTGCGCGGACACCGCCGGGCCTC
GTCGGGAGACCGGCTCAAGGACTCGGGCTCCGTGCAGAGACTCTCCACCTACGACAATGT
GCCCGCGCCGGGCCTGGTCCCCGGCATACCCAGCGTGGCCAGTATGGCGTGGTCCGGGGC
CTCGTCCAGCGAGTCGTCGGTGGGGGGCTCACTCAGCAGCTGCACGGCCTGCCGCGCCAG
CGACTCGTCTGCCCGCAGTTCCCTGCACACCGACTGGGCCCTGGAGCCCTCCCCGCTCCC
CAGCAGCAGCGAGGACCCCAAGTCCCTGGACCTGGACCACAGCATGGACGAGGCGGGCGC
GGGTGCCAGCAACAGCGAGCCCAGCGAGCCGGACAGCCCCACCCGGGAACACGCGCGCCG
CTCCGAGGCCTTACAGGGGCTGGTCACTGAGCTCAGGGCCGAGCTGTGCCGCCAGCGGAC
TGAGTACGAGAGGAGTGTGAAAAGAATCGAAGAAGGGAGTGCTGACCTGAGAAAACGAAT
GTCCCGGTTAGAAGAAGAACTGGACCAGGAAAAGAAAAAATACATCATGCTGGAAATAAA
GCTGCGGAACTCTGAACGGGCGCGGGAGGATGCGGAGAGGAGGAACCAGCTGTTGCAGAG
GGAAATGGAGGAGTTTTTTTCGACCCTAGGAAGCTTGACTGTTGGGGCAAAAGGTGCCAG
GGCCCCAAAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT