(Circular) (Six-base) MAPSORT of: pFN21AB9470.seq Check: 9250 from: 1 to: 6670 pFN21AB9470 6670 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4480 278 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4013 1937 Size: 2076 4594 Acc65I G'GTAC_C Cuts at: 3991 3991 Size: 6670 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2141 2156 2588 4733 6117 6385 1632 Size: 509 15 432 2145 1384 268 1917 Fragments arranged by size: 2145 1917 1384 509 432 268 15 AfeI AGC'GCT Cuts at: 2174 2174 Size: 6670 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6473 AflIII A'CryG_T Cuts at: 1203 5569 6546 1203 Size: 4366 977 1327 Fragments arranged by size: 4366 1327 977 AgeI A'CCGG_T Cuts at: 2929 5501 2929 Size: 2572 4098 AhdI GACnn_n'nnGTC Cuts at: 3559 5399 3559 Size: 1840 4830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6670 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3518 1184 Size: 2334 4336 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2438 2470 3254 3286 2438 Size: 32 784 32 5822 Fragments arranged by size: 5822 784 32 32 AlwNI CAG_nnn'CTG Cuts at: 2797 2884 3148 3459 3679 4413 5985 2797 Size: 87 264 311 220 734 1572 3482 Fragments arranged by size: 3482 1572 734 311 264 220 87 ApaI G_GGCC'C Cuts at: 2041 2114 2946 3520 3964 2041 Size: 73 832 574 444 4747 Fragments arranged by size: 4747 832 574 444 73 ApaLI G'TGCA_C Cuts at: 2546 4727 5883 2546 Size: 2181 1156 3333 Fragments arranged by size: 3333 2181 1156 ApoI r'AATT_y Cuts at: 1493 3979 4205 4357 6466 1493 Size: 2486 226 152 2109 1697 Fragments arranged by size: 2486 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5224 160 Size: 872 4192 1606 Fragments arranged by size: 4192 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 2288 2591 3322 3642 3995 1870 Size: 73 345 303 731 320 353 4545 Fragments arranged by size: 4545 731 353 345 320 303 73 AvrII C'CTAG_G Cuts at: 3925 3925 Size: 6670 BamHI G'GATC_C Cuts at: 4000 4367 4000 Size: 367 6303 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2389 2926 3226 3602 3953 Size: 325 217 627 602 537 300 376 351 Cuts at: 3953 3991 5446 618 Size: 38 1455 1842 Fragments arranged by size: 1842 1455 627 602 537 376 351 325 300 217 38 BanII G_rGCy'C Cuts at: 1391 2015 2041 2081 2114 2439 2589 2598 2946 Size: 624 26 40 33 325 150 9 348 Cuts at: 2946 2990 3024 3329 3449 3520 3528 3621 3693 Size: 44 34 305 120 71 8 93 72 Cuts at: 3693 3964 3989 1391 Size: 271 25 4072 Fragments arranged by size: 4072 624 348 325 305 271 150 120 93 72 71 44 40 34 33 26 25 9 8 BbeI G_GCGC'C Cuts at: 2393 2393 Size: 6670 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5699 BbvCI CC'TCA_GC Cuts at: 2630 2630 Size: 6670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2854 2888 3470 3504 4861 Size: 34 50 34 833 34 582 34 1357 Cuts at: 4861 4895 1903 Size: 34 3678 Fragments arranged by size: 3678 1357 833 582 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4562 5772 4562 Size: 1210 5460 BclI T'GATC_A Cuts at: 1548 1548 Size: 6670 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 3404 5281 Size: 107 122 71 631 605 364 1368 1877 Cuts at: 5281 136 Size: 1525 Fragments arranged by size: 1877 1525 1368 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 6665 6665 Size: 6670 BlpI GC'TnA_GC Cuts at: 2009 4087 2009 Size: 2078 4592 Bme1580I G_kGCm'C Cuts at: 1790 2041 2099 2114 2550 2672 2946 3231 3363 Size: 251 58 15 436 122 274 285 132 Cuts at: 3363 3520 3964 4731 5887 1790 Size: 157 444 767 1156 2573 Fragments arranged by size: 2573 1156 767 444 436 285 274 251 157 132 122 58 15 BmgBI CAC'GTC Cuts at: 1318 2545 1318 Size: 1227 5443 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2840 3147 3523 Size: 416 53 83 399 24 1423 307 376 Cuts at: 3523 4401 5359 442 Size: 878 958 1753 Fragments arranged by size: 1753 1423 958 878 416 399 376 307 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3329 3329 Size: 6670 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2696 3073 3242 3542 5330 1239 Size: 1457 377 169 300 1788 2579 Fragments arranged by size: 2579 1788 1457 377 300 169 Bpu10I CC'TnA_GC Cuts at: 2630 3692 2630 Size: 1062 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3299 4795 5660 5958 3299 Size: 1496 865 298 4011 Fragments arranged by size: 4011 1496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3300 5333 882 Size: 617 1801 2033 2219 Fragments arranged by size: 2219 2033 1801 617 BsaAI yAC'GTr Cuts at: 493 1204 4461 493 Size: 711 3257 2702 Fragments arranged by size: 3257 2702 711 BsaBI GATnn'nnATC Cuts at: 6664 6664 Size: 6670 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2390 4477 4859 5494 Size: 53 83 186 936 857 2087 382 635 Cuts at: 5494 275 Size: 1451 Fragments arranged by size: 2087 1451 936 857 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2929 5103 5501 5775 5922 6333 2929 Size: 2174 398 274 147 411 3266 Fragments arranged by size: 3266 2174 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 3311 3341 1178 Size: 30 165 30 1035 30 843 30 4507 Fragments arranged by size: 4507 1035 843 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2161 2459 3008 3229 3745 3892 3923 1186 Size: 975 298 549 221 516 147 31 3933 Fragments arranged by size: 3933 975 549 516 298 221 147 31 BseYI C'CCAG_C Cuts at: 2107 2640 3389 3539 3620 5873 6369 2107 Size: 533 749 150 81 2253 496 2408 Fragments arranged by size: 2408 2253 749 533 496 150 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3142 3161 3353 3445 3488 1163 Size: 573 1406 19 192 92 43 4345 Fragments arranged by size: 4345 1406 573 192 92 43 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3075 4120 4881 5909 1540 Size: 275 186 1074 1045 761 1028 2301 Fragments arranged by size: 2301 1074 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2550 2589 3087 3693 4731 4816 5887 2550 Size: 39 498 606 1038 85 1071 3333 Fragments arranged by size: 3333 1071 1038 606 498 85 39 BsmI GAATG_Cn' Cuts at: 4181 4274 4181 Size: 93 6577 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2041 2081 2099 2114 2439 2550 Size: 399 225 26 40 18 15 325 111 Cuts at: 2550 2589 2598 2672 2946 2990 3024 3087 3231 Size: 39 9 74 274 44 34 63 144 Cuts at: 3231 3329 3363 3449 3520 3528 3621 3693 3964 Size: 98 34 86 71 8 93 72 271 Cuts at: 3964 3989 4731 4816 5887 1391 Size: 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 325 274 271 225 144 111 98 93 86 85 74 72 71 63 44 40 39 34 34 26 25 18 15 9 8 BspEI T'CCGG_A Cuts at: 6333 6333 Size: 6670 BspHI T'CATG_A Cuts at: 2154 4559 6289 2154 Size: 2405 1730 2535 Fragments arranged by size: 2535 2405 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2499 2586 2730 4025 844 Size: 1655 87 144 1295 3489 Fragments arranged by size: 3489 1655 1295 144 87 BsrBI CCG'CTC Cuts at: 1394 1823 2043 2368 3535 3660 4557 1394 Size: 429 220 325 1167 125 897 3507 Fragments arranged by size: 3507 1167 897 429 325 220 125 BsrDI GCAATG_nn' Cuts at: 65 1248 2975 5165 5339 65 Size: 1183 1727 2190 174 1396 Fragments arranged by size: 2190 1727 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2929 3117 3309 5314 5501 1700 Size: 110 1119 188 192 2005 187 2869 Fragments arranged by size: 2869 2005 1119 192 188 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6670 BssHII G'CGCG_C Cuts at: 1925 3653 1925 Size: 1728 4942 BssSI C'ACGA_G Cuts at: 2363 4730 5742 2363 Size: 2367 1012 3291 Fragments arranged by size: 3291 2367 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3148 3468 3953 794 Size: 2354 320 485 3511 Fragments arranged by size: 3511 2354 485 320 BstBI TT'CG_AA Cuts at: 5474 5474 Size: 6670 BstEII G'GTnAC_C Cuts at: 1184 2998 1184 Size: 1814 4856 BstXI CCAn_nnnn'nTGG Cuts at: 3565 3565 Size: 6670 BstYI r'GATC_y Cuts at: 1972 2328 4000 4367 4752 4769 6221 6307 6319 Size: 356 1672 367 385 17 1452 86 12 Cuts at: 6319 6665 1972 Size: 346 1977 Fragments arranged by size: 1977 1672 1452 385 367 356 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2706 2706 Size: 6670 BtgI C'CryG_G Cuts at: 513 1065 2598 3075 3102 513 Size: 552 1533 477 27 4081 Fragments arranged by size: 4081 1533 552 477 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5931 Fragments arranged by size: 5931 683 56 BtsI GCAGTG_nn' Cuts at: 2414 2982 4186 4980 5000 2414 Size: 568 1204 794 20 4084 Fragments arranged by size: 4084 1204 794 568 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6635 DraI TTT'AAA Cuts at: 3974 3974 Size: 6670 DraIII CAC_nnn'GTG Cuts at: 2554 2554 Size: 6670 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5677 809 Size: 507 4361 1802 Fragments arranged by size: 4361 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2828 3072 3396 Size: 54 1425 50 275 18 998 244 324 Cuts at: 3396 4117 5006 6635 8 Size: 721 889 1629 43 Fragments arranged by size: 1629 1425 998 889 721 324 275 244 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3072 4117 1537 Size: 275 1260 1045 4090 Fragments arranged by size: 4090 1260 1045 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2924 3061 4600 1353 Size: 1571 137 1539 3423 Fragments arranged by size: 3423 1571 1539 137 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2101 5257 5631 5777 387 Size: 1064 138 512 3156 374 146 1280 Fragments arranged by size: 3156 1280 1064 512 374 146 138 EcoICRI GAG'CTC Cuts at: 2587 3691 2587 Size: 1104 5566 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2156 2588 2696 3073 3242 3542 Size: 393 509 15 432 108 377 169 300 Cuts at: 3542 4733 5330 6117 6385 1239 Size: 1191 597 787 268 1524 Fragments arranged by size: 1524 1191 787 597 509 432 393 377 300 268 169 108 15 EcoNI CCTnn'n_nnAGG Cuts at: 2611 2705 2611 Size: 94 6576 EcoO109I rG'GnC_Cy Cuts at: 2037 2111 2293 2488 2842 2942 2962 3051 3183 Size: 74 182 195 354 100 20 89 132 Cuts at: 3183 3417 3517 3553 3960 3961 2037 Size: 234 100 36 407 1 4746 Fragments arranged by size: 4746 407 354 234 195 182 132 100 100 89 74 36 20 1 EcoRI G'AATT_C Cuts at: 3979 4357 3979 Size: 378 6292 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2326 3184 2326 Size: 858 5812 FspI TGC'GCA Cuts at: 2974 5176 2974 Size: 2202 4468 HaeII r_GCGC'y Cuts at: 1678 2176 2344 2393 5817 1678 Size: 498 168 49 3424 2531 Fragments arranged by size: 3424 2531 498 168 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3311 3343 3545 3577 5311 Size: 32 135 32 1739 32 202 32 1734 Cuts at: 5311 5343 5385 5417 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1739 1734 202 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2776 4014 4260 669 Size: 988 281 838 1238 246 3079 Fragments arranged by size: 3079 1238 988 838 281 246 HindIII A'AGCT_T Cuts at: 748 3931 748 Size: 3183 3487 HpaI GTT'AAC Cuts at: 4260 4260 Size: 6670 KasI G'GCGC_C Cuts at: 2389 2389 Size: 6670 KpnI G_GTAC'C Cuts at: 3995 3995 Size: 6670 MfeI C'AATT_G Cuts at: 4269 4269 Size: 6670 MluI A'CGCG_T Cuts at: 6546 6546 Size: 6670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3137 5784 5968 1305 Size: 1832 2647 184 2007 Fragments arranged by size: 2647 2007 1832 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3398 10 Size: 54 1425 343 1566 3282 Fragments arranged by size: 3282 1566 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4628 4987 5146 518 Size: 648 3462 359 159 2042 Fragments arranged by size: 3462 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2634 2644 3459 3715 3889 4086 4763 5911 Size: 1028 10 815 256 174 197 677 1148 Cuts at: 5911 6156 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1028 815 677 256 245 197 174 10 NarI GG'CG_CC Cuts at: 2390 2390 Size: 6670 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6118 NdeI CA'TA_TG Cuts at: 387 387 Size: 6670 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 4117 4117 Size: 6670 NspI r_CATG'y Cuts at: 1132 1229 2464 4028 5573 1132 Size: 97 1235 1564 1545 2229 Fragments arranged by size: 2229 1564 1545 1235 97 PasI CC'CwG_GG Cuts at: 1348 2081 2236 2297 2324 3128 1348 Size: 733 155 61 27 804 4890 Fragments arranged by size: 4890 804 733 155 61 27 PciI A'CATG_T Cuts at: 5569 5569 Size: 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 2797 3583 1108 Size: 1689 786 4195 Fragments arranged by size: 4195 1689 786 PfoI T'CCnGG_A Cuts at: 2424 2934 2424 Size: 510 6160 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2438 2470 4739 4771 6252 6284 2438 Size: 32 2269 32 1481 32 2824 Fragments arranged by size: 2824 2269 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2488 2842 3051 3183 3553 2293 Size: 195 354 209 132 370 5410 Fragments arranged by size: 5410 370 354 209 195 132 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6670 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PspOMI G'GGCC_C Cuts at: 2037 2110 2942 3516 3960 2037 Size: 73 832 574 444 4747 Fragments arranged by size: 4747 832 574 444 73 PstI C_TGCA'G Cuts at: 830 2496 4022 830 Size: 1666 1526 3478 Fragments arranged by size: 3478 1666 1526 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 PvuII CAG'CTG Cuts at: 2634 2644 3459 3889 2634 Size: 10 815 430 5415 Fragments arranged by size: 5415 815 430 10 SacI G_AGCT'C Cuts at: 2589 3693 2589 Size: 1104 5566 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SapI GCTCTTCn'nnn_ Cuts at: 2924 2924 Size: 6670 SbfI CC_TGCA'GG Cuts at: 2496 4022 2496 Size: 1526 5144 ScaI AGT'ACT Cuts at: 4918 6630 4918 Size: 1712 4958 SexAI A'CCwGG_T Cuts at: 2478 2478 Size: 6670 SfcI C'TryA_G Cuts at: 826 1045 2492 4018 5153 5834 6025 826 Size: 219 1447 1526 1135 681 191 1471 Fragments arranged by size: 1526 1471 1447 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2036 2036 Size: 6670 SfoI GGC'GCC Cuts at: 2391 2391 Size: 6670 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 3644 3997 1872 Size: 1772 353 4545 Fragments arranged by size: 4545 1772 353 SmlI C'TyrA_G Cuts at: 820 1017 1943 3314 4774 5675 5937 820 Size: 197 926 1371 1460 901 262 1553 Fragments arranged by size: 1553 1460 1371 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6670 SphI G_CATG'C Cuts at: 1132 2464 4028 1132 Size: 1332 1564 3774 Fragments arranged by size: 3774 1564 1332 SspI AAT'ATT Cuts at: 5 52 4594 5 Size: 47 4542 2081 Fragments arranged by size: 4542 2081 47 StuI AGG'CCT Cuts at: 1517 3668 1517 Size: 2151 4519 StyI C'CwwG_G Cuts at: 513 1065 2652 3013 3925 4109 513 Size: 552 1587 361 912 184 3074 Fragments arranged by size: 3074 1587 912 552 361 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4697 4714 4867 5052 5497 325 Size: 1255 3117 17 153 185 445 1498 Fragments arranged by size: 3117 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4916 6628 96 Size: 274 80 33 51 1443 2939 1712 138 Fragments arranged by size: 2939 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6630 6662 6630 Size: 32 6638 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6670 XbaI T'CTAG_A Cuts at: 4006 4006 Size: 6670 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6670 XmaI C'CCGG_G Cuts at: 1870 3642 3995 1870 Size: 1772 353 4545 Fragments arranged by size: 4545 1772 353 XmnI GAAnn'nnTTC Cuts at: 4799 4799 Size: 6670 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4478 276 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SrfI SwaI XcmI