B4GALT5 ORF clone pFN21AB9394

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Halotag ORF Clone pFN21AB9394

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Clone info.

Species

human

Clone Name

pFN21AB9394 Sequence (with vector) Restriction Map

Product ID

FHC03579

Flexi Clone PID

FXC03579

ORF Sequence

DNA sequence (1164 bp)
Protein sequence (388 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527656]

Gene

KIBB9394

Symbol

B4GALT5

Alias : B4Gal-T5, BETA4-GALT-IV, beta4Gal-T5, beta4GalT-V, gt-V

Description

UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5

Original Clone ID

hj01540b

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1164 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 388 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13420.1 8.9e-185 100.0 B4GALT5 CCDS11900.1 4.5e-134 70.6 B4GALT6 CCDS82245.1 6.7e-114 65.2 B4GALT6 CCDS1222.1 1.5e-54 39.6 B4GALT3 CCDS6535.1 4e-53 43.6 B4GALT1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003859 198 217 PR02050 Galactosyltransferase IPR003859 229 248 PR02050 Galactosyltransferase IPR003859 266 287 PR02050 Galactosyltransferase IPR003859 288 306 PR02050 Galactosyltransferase HMMPfam IPR003859 114 378 PF02709 Galactosyltransferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 21 FFSLSSSLLYFVYVAPGIVNTYL 43 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004767 388 604016 100.0 100.0 NP_004766 382 604017 70.6 100.0 XP_005258444 370 604017 71.1 89.9 XP_016881578 338 604017 70.6 88.7 XP_006722642 338 604017 70.6 88.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9394 Download

>KIBB9394  1164 bp
ATGCGCGCCCGCCGGGGGCTGCTGCGGCTGCCGCGCCGCTCGCTGCTCGCCGCGCTCTTC
TTCTTTTCTCTCTCGTCCTCGCTGCTGTACTTCGTCTATGTGGCGCCCGGCATAGTGAAC
ACCTACCTCTTCATGATGCAAGCCCAAGGCATTCTGATCCGGGACAACGTGAGAACAATC
GGTGCTCAGGTTTATGAGCAGGTGCTTCGGAGTGCTTATGCCAAGAGGAACAGCAGTGTA
AATGACTCAGATTATCCTCTTGACTTGAACCACAGTGAAACCTTCCTGCAAACTACAACA
TTTCTTCCTGAAGACTTCACCTACTTTGCAAACCATACCTGCCCTGAAAGACTCCCTTCC
ATGAAGGGCCCAATAGACATAAACATGAGTGAAATTGGAATGGATTACATTCATGAACTC
TTCTCCAAAGACCCAACCATCAAGCTCGGAGGTCACTGGAAGCCTTCTGATTGCATGCCT
CGGTGGAAGGTGGCGATCCTTATCCCCTTCCGGAACCGCCACGAGCACCTCCCAGTCCTG
TTCAGACACCTGCTTCCCATGCTCCAGCGCCAGCGCCTGCAGTTTGCATTTTATGTGGTT
GAACAAGTTGGTACCCAACCCTTTAATCGAGCCATGCTTTTCAACGTTGGCTTTCAAGAA
GCAATGAAAGACTTGGATTGGGACTGTTTGATTTTTCATGATGTAGATCACATACCGGAA
AGTGATCGCAACTATTATGGATGTGGACAGATGCCGAGGCATTTTGCAACCAAATTGGAT
AAGTATATGTATCTGCTTCCTTATACCGAGTTCTTTGGCGGAGTGAGTGGCTTAACAGTG
GAACAATTTCGGAAAATCAATGGCTTTCCTAATGCTTTCTGGGGTTGGGGTGGAGAAGAT
GACGACCTCTGGAACAGAGTACAGAATGCAGGCTATTCTGTGAGCCGGCCAGAGGGTGAC
ACAGGAAAGTACAAGTCCATTCCTCATCACCATCGAGGAGAAGTCCAGTTTCTTGGAAGG
TATGCTCTGCTGAGGAAGTCAAAAGAACGGCAAGGGCTGGATGGCCTCAACAACCTGAAC
TACTTTGCAAACATCACATACGACGCCTTGTATAAAAACATAACTGTCAACCTGACACCC
GAGCTGGCTCAGGTGAACGAGTAC

ORF Protein

Cloned ORF protein sequence for pFN21AB9394 Download

>KIBB9394  388 aa
MRARRGLLRLPRRSLLAALFFFSLSSSLLYFVYVAPGIVNTYLFMMQAQGILIRDNVRTI
GAQVYEQVLRSAYAKRNSSVNDSDYPLDLNHSETFLQTTTFLPEDFTYFANHTCPERLPS
MKGPIDINMSEIGMDYIHELFSKDPTIKLGGHWKPSDCMPRWKVAILIPFRNRHEHLPVL
FRHLLPMLQRQRLQFAFYVVEQVGTQPFNRAMLFNVGFQEAMKDLDWDCLIFHDVDHIPE
SDRNYYGCGQMPRHFATKLDKYMYLLPYTEFFGGVSGLTVEQFRKINGFPNAFWGWGGED
DDLWNRVQNAGYSVSRPEGDTGKYKSIPHHHRGEVQFLGRYALLRKSKERQGLDGLNNLN
YFANITYDALYKNITVNLTPELAQVNEY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9394 Download

>pFN21AB9394  5869 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCGCGCCCGCCGGGGGCTGCTGCGGCTGCCGCG
CCGCTCGCTGCTCGCCGCGCTCTTCTTCTTTTCTCTCTCGTCCTCGCTGCTGTACTTCGT
CTATGTGGCGCCCGGCATAGTGAACACCTACCTCTTCATGATGCAAGCCCAAGGCATTCT
GATCCGGGACAACGTGAGAACAATCGGTGCTCAGGTTTATGAGCAGGTGCTTCGGAGTGC
TTATGCCAAGAGGAACAGCAGTGTAAATGACTCAGATTATCCTCTTGACTTGAACCACAG
TGAAACCTTCCTGCAAACTACAACATTTCTTCCTGAAGACTTCACCTACTTTGCAAACCA
TACCTGCCCTGAAAGACTCCCTTCCATGAAGGGCCCAATAGACATAAACATGAGTGAAAT
TGGAATGGATTACATTCATGAACTCTTCTCCAAAGACCCAACCATCAAGCTCGGAGGTCA
CTGGAAGCCTTCTGATTGCATGCCTCGGTGGAAGGTGGCGATCCTTATCCCCTTCCGGAA
CCGCCACGAGCACCTCCCAGTCCTGTTCAGACACCTGCTTCCCATGCTCCAGCGCCAGCG
CCTGCAGTTTGCATTTTATGTGGTTGAACAAGTTGGTACCCAACCCTTTAATCGAGCCAT
GCTTTTCAACGTTGGCTTTCAAGAAGCAATGAAAGACTTGGATTGGGACTGTTTGATTTT
TCATGATGTAGATCACATACCGGAAAGTGATCGCAACTATTATGGATGTGGACAGATGCC
GAGGCATTTTGCAACCAAATTGGATAAGTATATGTATCTGCTTCCTTATACCGAGTTCTT
TGGCGGAGTGAGTGGCTTAACAGTGGAACAATTTCGGAAAATCAATGGCTTTCCTAATGC
TTTCTGGGGTTGGGGTGGAGAAGATGACGACCTCTGGAACAGAGTACAGAATGCAGGCTA
TTCTGTGAGCCGGCCAGAGGGTGACACAGGAAAGTACAAGTCCATTCCTCATCACCATCG
AGGAGAAGTCCAGTTTCTTGGAAGGTATGCTCTGCTGAGGAAGTCAAAAGAACGGCAAGG
GCTGGATGGCCTCAACAACCTGAACTACTTTGCAAACATCACATACGACGCCTTGTATAA
AAACATAACTGTCAACCTGACACCCGAGCTGGCTCAGGTGAACGAGTACGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT