(Circular) (Six-base) MAPSORT of: pFN21AB9394.seq Check: 2655 from: 1 to: 5869 pFN21AB9394 5869 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2194 2562 2194 Size: 368 5501 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3679 278 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3212 1937 Size: 1275 4594 Acc65I G'GTAC_C Cuts at: 2615 3190 2615 Size: 575 5294 AclI AA'CG_TT Cuts at: 1563 2649 3996 4369 1563 Size: 1086 1347 373 3063 Fragments arranged by size: 3063 1347 1086 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 3932 5316 5584 1632 Size: 702 1598 1384 268 1917 Fragments arranged by size: 1917 1598 1384 702 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 4768 5745 1203 Size: 3565 977 1327 Fragments arranged by size: 3565 1327 977 AgeI A'CCGG_T Cuts at: 4700 4700 Size: 5869 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5869 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5869 AlwNI CAG_nnn'CTG Cuts at: 2581 3612 5184 2581 Size: 1031 1572 3266 Fragments arranged by size: 3266 1572 1031 ApaI G_GGCC'C Cuts at: 2375 2375 Size: 5869 ApaLI G'TGCA_C Cuts at: 3926 5082 3926 Size: 1156 4713 ApoI r'AATT_y Cuts at: 1493 3178 3404 3556 5665 1493 Size: 1685 226 152 2109 1697 Fragments arranged by size: 2109 1697 1685 226 152 AseI AT'TA_AT Cuts at: 160 1032 4423 160 Size: 872 3391 1606 Fragments arranged by size: 3391 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 3143 3194 1870 Size: 73 1200 51 4545 Fragments arranged by size: 4545 1200 73 51 BamHI G'GATC_C Cuts at: 3199 3566 3199 Size: 367 5502 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2107 2615 3190 4645 618 Size: 325 217 627 320 508 575 1455 1842 Fragments arranged by size: 1842 1455 627 575 508 325 320 217 BanII G_rGCy'C Cuts at: 1391 2375 3188 1391 Size: 984 813 4072 Fragments arranged by size: 4072 984 813 BbeI G_GCGC'C Cuts at: 2111 2111 Size: 5869 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 928 Size: 971 423 4475 Fragments arranged by size: 4475 971 423 BbvCI CC'TCA_GC Cuts at: 3035 3035 Size: 5869 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2192 2226 4060 4094 1903 Size: 34 50 34 171 34 1834 34 3678 Fragments arranged by size: 3678 1834 171 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3761 4971 3761 Size: 1210 4659 BclI T'GATC_A Cuts at: 1548 1548 Size: 5869 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4480 136 Size: 107 122 71 631 605 2808 1525 Fragments arranged by size: 2808 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5864 5864 Size: 5869 BlpI GC'TnA_GC Cuts at: 3286 3286 Size: 5869 Bme1580I G_kGCm'C Cuts at: 1790 2375 3930 5086 1790 Size: 585 1555 1156 2573 Fragments arranged by size: 2573 1555 1156 585 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5869 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2531 3600 4558 Size: 416 53 83 399 24 1114 1069 958 Cuts at: 4558 442 Size: 1753 Fragments arranged by size: 1753 1114 1069 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2552 4529 1239 Size: 1313 1977 2579 Fragments arranged by size: 2579 1977 1313 Bpu10I CC'TnA_GC Cuts at: 2190 3035 3153 2190 Size: 845 118 4906 Fragments arranged by size: 4906 845 118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3994 4859 5157 3994 Size: 865 298 4706 Fragments arranged by size: 4706 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4532 882 Size: 617 3033 2219 Fragments arranged by size: 3033 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3660 493 Size: 711 2456 2702 Fragments arranged by size: 2702 2456 711 BsaBI GATnn'nnATC Cuts at: 2504 2709 5863 2504 Size: 205 3154 2510 Fragments arranged by size: 3154 2510 205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2108 3108 3676 4058 Size: 53 83 186 936 575 1000 568 382 Cuts at: 4058 4693 275 Size: 635 1451 Fragments arranged by size: 1451 1000 936 635 575 568 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2514 2719 4302 4700 4974 5121 5532 2514 Size: 205 1583 398 274 147 411 2851 Fragments arranged by size: 2851 1583 411 398 274 205 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5644 Fragments arranged by size: 5644 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3015 1186 Size: 1829 4040 BseYI C'CCAG_C Cuts at: 5072 5568 5072 Size: 496 5373 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5296 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3319 4080 5108 1540 Size: 275 186 1318 761 1028 2301 Fragments arranged by size: 2301 1318 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2191 2532 3930 4015 5086 2191 Size: 341 1398 85 1071 2974 Fragments arranged by size: 2974 1398 1071 341 85 BsmI GAATG_Cn' Cuts at: 2154 2935 3380 3473 2154 Size: 781 445 93 4550 Fragments arranged by size: 4550 781 445 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2191 2375 2532 3188 3930 4015 5086 Size: 399 401 184 157 656 742 85 1071 Cuts at: 5086 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 656 401 399 184 157 85 BspEI T'CCGG_A Cuts at: 2514 5532 2514 Size: 3018 2851 BspHI T'CATG_A Cuts at: 2136 2416 2701 3758 5488 2136 Size: 280 285 1057 1730 2517 Fragments arranged by size: 2517 1730 1057 285 280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2194 2351 2562 3224 844 Size: 1350 157 211 662 3489 Fragments arranged by size: 3489 1350 662 211 157 BsrBI CCG'CTC Cuts at: 1394 1823 2043 3756 1394 Size: 429 220 1713 3507 Fragments arranged by size: 3507 1713 429 220 BsrDI GCAATG_nn' Cuts at: 65 1248 2673 4364 4538 65 Size: 1183 1425 1691 174 1396 Fragments arranged by size: 1691 1425 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2949 4513 4700 1700 Size: 110 1139 1564 187 2869 Fragments arranged by size: 2869 1564 1139 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5869 BssHII G'CGCG_C Cuts at: 1925 2008 1925 Size: 83 5786 BssSI C'ACGA_G Cuts at: 2525 3929 4941 2525 Size: 1404 1012 3453 Fragments arranged by size: 3453 1404 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5869 BstBI TT'CG_AA Cuts at: 4673 4673 Size: 5869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstXI CCAn_nnnn'nTGG Cuts at: 3017 3017 Size: 5869 BstYI r'GATC_y Cuts at: 1972 3199 3566 3951 3968 5420 5506 5518 5864 Size: 1227 367 385 17 1452 86 12 346 Cuts at: 5864 1972 Size: 1977 Fragments arranged by size: 1977 1452 1227 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5317 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5130 Fragments arranged by size: 5130 683 56 BtsI GCAGTG_nn' Cuts at: 2245 3385 4179 4199 2245 Size: 1140 794 20 3915 Fragments arranged by size: 3915 1140 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 3173 3173 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4876 809 Size: 507 3560 1802 Fragments arranged by size: 3560 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2951 3316 4205 Size: 54 1425 50 275 18 1121 365 889 Cuts at: 4205 5834 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1121 889 365 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3316 1537 Size: 275 1504 4090 Fragments arranged by size: 4090 1504 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2138 2429 3799 1353 Size: 713 72 291 1370 3423 Fragments arranged by size: 3423 1370 713 291 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2838 4456 4830 4976 387 Size: 1064 138 1249 1618 374 146 1280 Fragments arranged by size: 1618 1280 1249 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 2552 3932 4529 5316 5584 1239 Size: 393 702 218 1380 597 787 268 1524 Fragments arranged by size: 1524 1380 787 702 597 393 268 218 EcoO109I rG'GnC_Cy Cuts at: 2371 2371 Size: 5869 EcoRI G'AATT_C Cuts at: 3178 3556 3178 Size: 378 5491 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2376 2376 Size: 5869 FspI TGC'GCA Cuts at: 4375 4375 Size: 5869 HaeII r_GCGC'y Cuts at: 1678 2111 2575 2581 5016 1678 Size: 433 464 6 2435 2531 Fragments arranged by size: 2531 2435 464 433 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2991 3023 4510 4542 4584 Size: 32 135 32 1419 32 1487 32 42 Cuts at: 4584 4616 1373 Size: 32 2626 Fragments arranged by size: 2626 1487 1419 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3133 3213 3459 669 Size: 988 281 1195 80 246 3079 Fragments arranged by size: 3079 1195 988 281 246 80 HindIII A'AGCT_T Cuts at: 748 748 Size: 5869 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KasI G'GCGC_C Cuts at: 2107 2107 Size: 5869 KpnI G_GTAC'C Cuts at: 2619 3194 2619 Size: 575 5294 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4983 5167 1305 Size: 3678 184 2007 Fragments arranged by size: 3678 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4047 Fragments arranged by size: 4047 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 3827 4186 4345 518 Size: 648 1221 1440 359 159 2042 Fragments arranged by size: 2042 1440 1221 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3285 3962 5110 5355 1606 Size: 1679 677 1148 245 2120 Fragments arranged by size: 2120 1679 1148 677 245 NaeI GCC'GGC Cuts at: 2951 2951 Size: 5869 NarI GG'CG_CC Cuts at: 2108 2108 Size: 5869 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5317 NdeI CA'TA_TG Cuts at: 387 387 Size: 5869 NgoMIV G'CCGG_C Cuts at: 2949 2949 Size: 5869 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 3316 3316 Size: 5869 NspI r_CATG'y Cuts at: 1132 1229 2482 3227 4772 1132 Size: 97 1253 745 1545 2229 Fragments arranged by size: 2229 1545 1253 745 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5869 PciI A'CATG_T Cuts at: 4768 4768 Size: 5869 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5869 PfoI T'CCnGG_A Cuts at: 2163 2163 Size: 5869 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 5451 5483 3938 Size: 32 1481 32 4324 Fragments arranged by size: 4324 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5869 PsiI TTA'TAA Cuts at: 3439 3439 Size: 5869 PspOMI G'GGCC_C Cuts at: 2371 2371 Size: 5869 PstI C_TGCA'G Cuts at: 830 2586 3221 830 Size: 1756 635 3478 Fragments arranged by size: 3478 1756 635 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 SalI G'TCGA_C Cuts at: 1936 3211 1936 Size: 1275 4594 SapI GCTCTTCn'nnn_ Cuts at: 2066 2066 Size: 5869 SbfI CC_TGCA'GG Cuts at: 3221 3221 Size: 5869 ScaI AGT'ACT Cuts at: 4117 5829 4117 Size: 1712 4157 SfcI C'TryA_G Cuts at: 826 1045 2582 3217 4352 5033 5224 826 Size: 219 1537 635 1135 681 191 1471 Fragments arranged by size: 1537 1471 1135 681 635 219 191 SfoI GGC'GCC Cuts at: 2109 2109 Size: 5869 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5869 SmaI CCC'GGG Cuts at: 1872 3196 1872 Size: 1324 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3973 4874 5136 820 Size: 197 926 2030 901 262 1553 Fragments arranged by size: 2030 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3660 493 Size: 3167 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5869 SphI G_CATG'C Cuts at: 1132 2482 3227 1132 Size: 1350 745 3774 Fragments arranged by size: 3774 1350 745 SspI AAT'ATT Cuts at: 5 52 3793 5 Size: 47 3741 2081 Fragments arranged by size: 3741 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5869 StyI C'CwwG_G Cuts at: 513 1065 2149 3308 513 Size: 552 1084 1159 3074 Fragments arranged by size: 3074 1159 1084 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3896 3913 4066 4251 4696 325 Size: 1255 2316 17 153 185 445 1498 Fragments arranged by size: 2316 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2091 2923 2973 Size: 274 80 33 51 1443 114 832 50 Cuts at: 2973 4115 5827 96 Size: 1142 1712 138 Fragments arranged by size: 1712 1443 1142 832 274 138 114 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2224 2256 5829 5861 2224 Size: 32 3573 32 2232 Fragments arranged by size: 3573 2232 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5869 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5869 XmaI C'CCGG_G Cuts at: 1870 3194 1870 Size: 1324 4545 XmnI GAAnn'nnTTC Cuts at: 2850 3998 2850 Size: 1148 4721 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3677 276 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI