GPR182 ORF clone pFN21AB8823

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Halotag ORF Clone pFN21AB8823

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Clone info.

Species

human

Clone Name

pFN21AB8823 Sequence (with vector) Restriction Map

Product ID

FHC03046

Flexi Clone PID

FXC03046

ORF Sequence

DNA sequence (1212 bp)
Protein sequence (404 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464542]

Gene

KIBB8823

Symbol

GPR182

Alias : 7TMR, ADMR, AM-R, AMR, G10D, L1-R, gamrh, hrhAMR

Description

G protein-coupled receptor 182

Original Clone ID

cp00858

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1212 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 404 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8927.1 6.8e-130 100.0 GPR182 CCDS2516.1 2.7e-28 34.2 ACKR3 CCDS2706.1 2.8e-23 32.7 ACKR2 CCDS2684.1 4.3e-20 28.1 CCR8 CCDS3137.1 7.7e-20 27.2 AGTR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001350 7 26 PR00643 G10D orphan receptor IPR001350 26 42 PR00643 G10D orphan receptor IPR001350 42 57 PR00643 G10D orphan receptor IPR000276 55 79 PR00237 GPCR IPR001350 76 87 PR00643 G10D orphan receptor IPR000276 88 109 PR00237 GPCR IPR000276 133 155 PR00237 GPCR IPR001350 154 171 PR00643 G10D orphan receptor IPR000276 169 190 PR00237 GPCR IPR001350 196 214 PR00643 G10D orphan receptor IPR000276 219 242 PR00237 GPCR IPR001350 243 257 PR00643 G10D orphan receptor IPR000276 256 280 PR00237 GPCR IPR001350 291 308 PR00643 G10D orphan receptor IPR000276 302 328 PR00237 GPCR IPR001350 331 350 PR00643 G10D orphan receptor HMMPfam IPR000276 70 320 PF00001 GPCR ProfileScan IPR017452 70 320 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 54 VVLFALYLAMFVVGLVENLLVIC 76 PRIMARY 23 2 88 MNLYILNMAIADLGIVLSLPVWM 110 PRIMARY 23 3 133 YFVNMYSSIFFLVCLSVDRYVTL 155 SECONDARY 23 4 172 AMCAGIWVLSAIIPLPEVVHIQL 194 PRIMARY 23 5 199 EPMCLFMAPFETYSTWALAVALS 221 SECONDARY 23 6 223 TILGFLLPFPLITVFNVLTACR 244 PRIMARY 22 7 259 LLCAYVAVFVMCWLPYHVTLLLL 281 PRIMARY 23 8 297 LLYFFYDVIDCFSMLHCVINPIL 319 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011536127 404 605307 100.0 100.0 NP_009195 404 605307 100.0 100.0 XP_011536126 404 605307 100.0 100.0 XP_011536129 404 605307 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8823 Download

>KIBB8823  1212 bp
ATGTCAGTGAAACCCAGCTGGGGGCCTGGCCCCTCGGAGGGGGTCACCGCAGTGCCTACC
AGTGACCTTGGAGAGATCCACAACTGGACCGAGCTGCTTGACCTCTTCAACCACACTTTG
TCTGAGTGCCACGTGGAGCTCAGCCAGAGCACCAAGCGCGTGGTCCTCTTTGCCCTCTAC
CTGGCCATGTTTGTGGTTGGGCTGGTGGAGAACCTCCTGGTGATATGCGTCAACTGGCGC
GGCTCAGGCCGGGCAGGGCTGATGAACCTCTACATCCTCAACATGGCCATCGCGGACCTG
GGCATTGTCCTGTCTCTGCCCGTGTGGATGCTGGAGGTCACGCTGGACTACACCTGGCTC
TGGGGCAGCTTCTCCTGCCGCTTCACTCACTACTTCTACTTTGTCAACATGTATAGCAGC
ATCTTCTTCCTGGTGTGCCTCAGTGTCGACCGCTATGTCACCCTCACCAGCGCCTCCCCC
TCCTGGCAGCGTTACCAGCACCGAGTGCGGCGGGCCATGTGTGCAGGCATCTGGGTCCTC
TCGGCCATCATCCCGCTGCCTGAGGTGGTCCACATCCAGCTGGTGGAGGGCCCTGAGCCC
ATGTGCCTCTTCATGGCACCTTTTGAAACGTACAGCACCTGGGCCCTGGCGGTGGCCCTG
TCCACCACCATCCTGGGCTTCCTGCTGCCCTTCCCTCTCATCACAGTCTTCAATGTGCTG
ACAGCCTGCCGGCTGCGGCAGCCAGGACAACCCAAGAGCCGGCGCCACTGCCTGCTGCTG
TGCGCCTACGTGGCCGTCTTTGTCATGTGCTGGCTGCCCTATCATGTGACCCTGCTGCTG
CTCACACTGCATGGGACCCACATCTCCCTCCACTGCCACCTGGTCCACCTGCTCTACTTC
TTCTATGATGTCATTGACTGCTTCTCCATGCTGCACTGTGTCATCAACCCCATCCTTTAC
AACTTTCTCAGCCCACACTTCCGGGGCCGGCTCCTGAATGCTGTAGTCCATTACCTTCCT
AAGGACCAGACCAAGGCGGGCACATGCGCCTCCTCTTCCTCCTGTTCCACCCAGCATTCC
ATCATCATCACCAAGGGTGATAGCCAGCCTGCTGCAGCAGCCCCCCACCCTGAGCCAAGC
CTGAGCTTTCAGGCACACCATTTGCTTCCAAATACTTCCCCCATCTCTCCCACTCAGCCT
CTTACACCCAGC

ORF Protein

Cloned ORF protein sequence for pFN21AB8823 Download

>KIBB8823  404 aa
MSVKPSWGPGPSEGVTAVPTSDLGEIHNWTELLDLFNHTLSECHVELSQSTKRVVLFALY
LAMFVVGLVENLLVICVNWRGSGRAGLMNLYILNMAIADLGIVLSLPVWMLEVTLDYTWL
WGSFSCRFTHYFYFVNMYSSIFFLVCLSVDRYVTLTSASPSWQRYQHRVRRAMCAGIWVL
SAIIPLPEVVHIQLVEGPEPMCLFMAPFETYSTWALAVALSTTILGFLLPFPLITVFNVL
TACRLRQPGQPKSRRHCLLLCAYVAVFVMCWLPYHVTLLLLTLHGTHISLHCHLVHLLYF
FYDVIDCFSMLHCVINPILYNFLSPHFRGRLLNAVVHYLPKDQTKAGTCASSSSCSTQHS
IIITKGDSQPAAAAPHPEPSLSFQAHHLLPNTSPISPTQPLTPS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8823 Download

>pFN21AB8823  5917 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCAGTGAAACCCAGCTGGGGGCCTGGCCCCTC
GGAGGGGGTCACCGCAGTGCCTACCAGTGACCTTGGAGAGATCCACAACTGGACCGAGCT
GCTTGACCTCTTCAACCACACTTTGTCTGAGTGCCACGTGGAGCTCAGCCAGAGCACCAA
GCGCGTGGTCCTCTTTGCCCTCTACCTGGCCATGTTTGTGGTTGGGCTGGTGGAGAACCT
CCTGGTGATATGCGTCAACTGGCGCGGCTCAGGCCGGGCAGGGCTGATGAACCTCTACAT
CCTCAACATGGCCATCGCGGACCTGGGCATTGTCCTGTCTCTGCCCGTGTGGATGCTGGA
GGTCACGCTGGACTACACCTGGCTCTGGGGCAGCTTCTCCTGCCGCTTCACTCACTACTT
CTACTTTGTCAACATGTATAGCAGCATCTTCTTCCTGGTGTGCCTCAGTGTCGACCGCTA
TGTCACCCTCACCAGCGCCTCCCCCTCCTGGCAGCGTTACCAGCACCGAGTGCGGCGGGC
CATGTGTGCAGGCATCTGGGTCCTCTCGGCCATCATCCCGCTGCCTGAGGTGGTCCACAT
CCAGCTGGTGGAGGGCCCTGAGCCCATGTGCCTCTTCATGGCACCTTTTGAAACGTACAG
CACCTGGGCCCTGGCGGTGGCCCTGTCCACCACCATCCTGGGCTTCCTGCTGCCCTTCCC
TCTCATCACAGTCTTCAATGTGCTGACAGCCTGCCGGCTGCGGCAGCCAGGACAACCCAA
GAGCCGGCGCCACTGCCTGCTGCTGTGCGCCTACGTGGCCGTCTTTGTCATGTGCTGGCT
GCCCTATCATGTGACCCTGCTGCTGCTCACACTGCATGGGACCCACATCTCCCTCCACTG
CCACCTGGTCCACCTGCTCTACTTCTTCTATGATGTCATTGACTGCTTCTCCATGCTGCA
CTGTGTCATCAACCCCATCCTTTACAACTTTCTCAGCCCACACTTCCGGGGCCGGCTCCT
GAATGCTGTAGTCCATTACCTTCCTAAGGACCAGACCAAGGCGGGCACATGCGCCTCCTC
TTCCTCCTGTTCCACCCAGCATTCCATCATCATCACCAAGGGTGATAGCCAGCCTGCTGC
AGCAGCCCCCCACCCTGAGCCAAGCCTGAGCTTTCAGGCACACCATTTGCTTCCAAATAC
TTCCCCCATCTCTCCCACTCAGCCTCTTACACCCAGCGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT