(Circular) (Six-base) MAPSORT of: pFN21AB8823.seq Check: 4712 from: 1 to: 5917 pFN21AB8823 5917 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2901 2901 Size: 5917 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2451 3260 1937 Size: 514 809 4594 Fragments arranged by size: 4594 809 514 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3980 5364 5632 1632 Size: 2348 1384 268 1917 Fragments arranged by size: 2348 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 2412 4816 5793 1203 Size: 1209 2404 977 1327 Fragments arranged by size: 2404 1327 1209 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 2054 1166 Size: 888 5029 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AlwNI CAG_nnn'CTG Cuts at: 2643 3660 5232 2643 Size: 1017 1572 3328 Fragments arranged by size: 3328 1572 1017 ApaI G_GGCC'C Cuts at: 2597 2650 2597 Size: 53 5864 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 3226 3452 3604 5713 1493 Size: 1733 226 152 2109 1697 Fragments arranged by size: 2109 1733 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 3242 1870 Size: 73 1299 4545 Fragments arranged by size: 4545 1299 73 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2620 2766 3238 4693 618 Size: 325 217 627 833 146 472 1455 1842 Fragments arranged by size: 1842 1455 833 627 472 325 217 146 BanII G_rGCy'C Cuts at: 1391 2145 2597 2604 2650 3236 1391 Size: 754 452 7 46 586 4072 Fragments arranged by size: 4072 754 586 452 46 7 BbeI G_GCGC'C Cuts at: 2770 2770 Size: 5917 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2704 928 Size: 971 805 4141 Fragments arranged by size: 4141 971 805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 1548 Size: 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2795 4528 136 Size: 107 122 71 631 605 1123 1733 1525 Fragments arranged by size: 1733 1525 1123 631 605 122 107 71 BglII A'GATC_T Cuts at: 5912 5912 Size: 5917 BlpI GC'TnA_GC Cuts at: 2144 3334 2144 Size: 1190 4727 Bme1580I G_kGCm'C Cuts at: 1790 2597 2650 3047 3978 5134 1790 Size: 807 53 397 931 1156 2573 Fragments arranged by size: 2573 1156 931 807 397 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3648 4606 442 Size: 416 53 83 399 24 2231 958 1753 Fragments arranged by size: 2231 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2357 4577 1239 Size: 1118 2220 2579 Fragments arranged by size: 2579 2220 1118 Bpu10I CC'TnA_GC Cuts at: 2248 2598 3135 3146 2248 Size: 350 537 11 5019 Fragments arranged by size: 5019 537 350 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4042 4907 5205 4042 Size: 865 298 4754 Fragments arranged by size: 4754 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4580 882 Size: 617 3081 2219 Fragments arranged by size: 3081 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2137 2794 3708 493 Size: 711 933 657 914 2702 Fragments arranged by size: 2702 933 914 711 657 BsaBI GATnn'nnATC Cuts at: 5911 5911 Size: 5917 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2767 3724 4106 4741 Size: 53 83 186 936 1234 957 382 635 Cuts at: 4741 275 Size: 1451 Fragments arranged by size: 1451 1234 957 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4350 4748 5022 5169 5580 4350 Size: 398 274 147 411 4687 Fragments arranged by size: 4687 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2912 2942 1178 Size: 30 165 30 1509 30 4153 Fragments arranged by size: 4153 1509 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3046 1186 Size: 1860 4057 BseYI C'CCAG_C Cuts at: 2018 2022 3075 3212 5120 5616 2018 Size: 4 1053 137 1908 496 2319 Fragments arranged by size: 2319 1908 1053 496 137 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2547 2921 1163 Size: 573 811 374 4159 Fragments arranged by size: 4159 811 573 374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2455 3367 4128 5156 1540 Size: 275 186 454 912 761 1028 2301 Fragments arranged by size: 2301 1028 912 761 454 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2156 3978 4063 5134 2145 Size: 11 1822 85 1071 2928 Fragments arranged by size: 2928 1822 1071 85 11 BsmI GAATG_Cn' Cuts at: 3007 3079 3428 3521 3007 Size: 72 349 93 5403 Fragments arranged by size: 5403 349 93 72 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2156 2597 2604 2650 3047 3236 Size: 399 355 11 441 7 46 397 189 Cuts at: 3236 3978 4063 5134 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 441 399 397 355 189 85 46 11 7 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2901 3272 844 Size: 2057 371 3489 Fragments arranged by size: 3489 2057 371 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4412 4586 65 Size: 1183 3164 174 1396 Fragments arranged by size: 3164 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2733 2763 2991 4561 4748 1700 Size: 110 923 30 228 1570 187 2869 Fragments arranged by size: 2869 1570 923 228 187 110 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 2509 794 Size: 1715 4202 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 2047 1184 Size: 863 5054 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2197 2677 2164 Size: 33 480 5404 Fragments arranged by size: 5404 480 33 BstYI r'GATC_y Cuts at: 1972 2079 3247 3614 3999 4016 5468 5554 5566 Size: 107 1168 367 385 17 1452 86 12 Cuts at: 5566 5912 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1168 385 367 346 107 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2565 3024 2565 Size: 459 5458 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5365 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2278 487 Size: 683 56 1052 4126 Fragments arranged by size: 4126 1052 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2770 2849 2875 3433 4227 4247 2061 Size: 709 79 26 558 794 20 3731 Fragments arranged by size: 3731 794 709 558 79 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DraIII CAC_nnn'GTG Cuts at: 2509 2509 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2459 4924 809 Size: 507 1143 2465 1802 Fragments arranged by size: 2465 1802 1143 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2289 2547 Size: 54 1425 50 275 18 357 102 258 Cuts at: 2547 2796 3364 4253 5882 8 Size: 249 568 889 1629 43 Fragments arranged by size: 1629 1425 889 568 357 275 258 249 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2618 3064 3847 1353 Size: 761 504 446 783 3423 Fragments arranged by size: 3423 783 761 504 446 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4504 4878 5024 387 Size: 1064 138 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2143 2143 Size: 5917 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 3980 4577 5364 5632 1239 Size: 393 725 1623 597 787 268 1524 Fragments arranged by size: 1623 1524 787 725 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3023 3023 Size: 5917 EcoO109I rG'GnC_Cy Cuts at: 2027 2539 2593 2594 2647 2859 2027 Size: 512 54 1 53 212 5085 Fragments arranged by size: 5085 512 212 54 53 1 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 2478 2770 5064 1678 Size: 800 292 2294 2531 Fragments arranged by size: 2531 2294 800 292 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2912 2944 4558 4590 4632 Size: 32 135 32 1340 32 1614 32 42 Cuts at: 4632 4664 1373 Size: 32 2626 Fragments arranged by size: 2626 1614 1340 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2236 2410 2452 3261 3507 669 Size: 988 281 298 174 42 809 246 3079 Fragments arranged by size: 3079 988 809 298 281 246 174 42 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KasI G'GCGC_C Cuts at: 2766 2766 Size: 5917 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5031 5215 1305 Size: 3726 184 2007 Fragments arranged by size: 3726 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2189 2291 10 Size: 54 1425 343 357 102 3636 Fragments arranged by size: 3636 1425 357 343 102 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2054 2195 3875 4234 4393 518 Size: 648 843 45 141 1680 359 159 2042 Fragments arranged by size: 2042 1680 843 648 359 159 141 45 MspA1I CmG'CkG Cuts at: 1606 2022 2560 2584 3333 4010 5158 5403 1606 Size: 416 538 24 749 677 1148 245 2120 Fragments arranged by size: 2120 1148 749 677 538 416 245 24 NaeI GCC'GGC Cuts at: 2735 2765 2993 2735 Size: 30 228 5659 Fragments arranged by size: 5659 228 30 NarI GG'CG_CC Cuts at: 2767 2767 Size: 5917 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5365 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NgoMIV G'CCGG_C Cuts at: 2733 2763 2991 2733 Size: 30 228 5659 Fragments arranged by size: 5659 228 30 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 2416 3051 3275 4820 1132 Size: 97 1187 635 224 1545 2229 Fragments arranged by size: 2229 1545 1187 635 224 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 2412 4816 2412 Size: 2404 3513 PflMI CCAn_nnn'nTGG Cuts at: 1108 2587 1108 Size: 1479 4438 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PmlI CAC'GTG Cuts at: 2137 2137 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2539 2859 2539 Size: 320 5597 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PspOMI G'GGCC_C Cuts at: 2593 2646 2593 Size: 53 5864 PstI C_TGCA'G Cuts at: 830 3121 3269 830 Size: 2291 148 3478 Fragments arranged by size: 3478 2291 148 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 PvuII CAG'CTG Cuts at: 2022 2584 2022 Size: 562 5355 SacI G_AGCT'C Cuts at: 2145 2145 Size: 5917 SalI G'TCGA_C Cuts at: 1936 2450 3259 1936 Size: 514 809 4594 Fragments arranged by size: 4594 809 514 SanDI GG'GwC_CC Cuts at: 2859 2859 Size: 5917 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 4165 5877 4165 Size: 1712 4205 SexAI A'CCwGG_T Cuts at: 2883 2883 Size: 5917 SfcI C'TryA_G Cuts at: 826 1045 3006 3117 3265 4400 5081 5272 826 Size: 219 1961 111 148 1135 681 191 1471 Fragments arranged by size: 1961 1471 1135 681 219 191 148 111 SfoI GGC'GCC Cuts at: 2768 2768 Size: 5917 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3244 1872 Size: 1372 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4021 4922 5184 820 Size: 197 926 2078 901 262 1553 Fragments arranged by size: 2078 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 2071 3036 3096 3356 513 Size: 552 1006 965 60 260 3074 Fragments arranged by size: 3074 1006 965 552 260 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2108 3089 3226 3944 3961 4114 4299 Size: 1255 528 981 137 718 17 153 185 Cuts at: 4299 4744 325 Size: 445 1498 Fragments arranged by size: 1498 1255 981 718 528 445 185 153 137 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4163 5875 96 Size: 274 80 33 51 1443 2186 1712 138 Fragments arranged by size: 2186 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5877 5909 5877 Size: 32 5885 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2299 2674 2299 Size: 375 5542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 3242 1870 Size: 1372 4545 XmnI GAAnn'nnTTC Cuts at: 4046 4046 Size: 5917 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI