GPR84 ORF clone pFN21AB8822

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB8822

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Clone info.

Species

human

Clone Name

pFN21AB8822 Sequence (with vector) Restriction Map

Product ID

FHC03045

Flexi Clone PID

FXC03045

ORF Sequence

DNA sequence (1188 bp)
Protein sequence (396 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464549]

Gene

KIBB8822

Symbol

GPR84

Alias : EX33, GPCR4

Description

G protein-coupled receptor 84

Original Clone ID

cp00857

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1188 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 396 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8878.1 4.3e-120 100.0 GPR84 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 22 46 PR00237 GPCR IPR000276 55 76 PR00237 GPCR IPR000276 100 122 PR00237 GPCR IPR000276 317 341 PR00237 GPCR IPR000276 352 378 PR00237 GPCR HMMPfam IPR000276 37 370 PF00001 GPCR ProfileScan IPR017452 37 370 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 21 YVAVSWGVVVAVTGTVGNVLTLL 43 SECONDARY 23 2 55 FNLLIANLTLADLLYCTLLQPFS 77 SECONDARY 23 3 97 GLLLFASNSVSILTLCLIALGRY 119 PRIMARY 23 4 141 LVSTWVVGVASFAPLWPIYILVP 163 PRIMARY 23 5 179 TTILMGIYFVLGLSSVGIFYCLI 201 PRIMARY 23 6 319 RMCFAVFLCFALSYIPFLLLNIL 341 PRIMARY 23 7 353 MLAANLTWLNGCINPVLYAAMNR 375 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011536797 396 606383 100.0 100.0 NP_065103 396 606383 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8822 Download

>KIBB8822  1188 bp
ATGTGGAACAGCTCTGACGCCAACTTCTCCTGCTACCATGAGTCTGTGCTGGGCTATCGT
TATGTTGCAGTTAGCTGGGGGGTGGTGGTGGCTGTGACAGGCACCGTGGGCAATGTGCTC
ACCCTACTGGCCTTGGCCATCCAGCCCAAGCTCCGTACCCGATTCAACCTGCTCATAGCC
AACCTCACACTGGCTGATCTCCTCTACTGCACGCTCCTTCAGCCCTTCTCTGTGGACACC
TACCTCCACCTGCACTGGCGCACCGGTGCCACCTTCTGCAGGGTATTTGGGCTCCTCCTT
TTTGCCTCCAATTCTGTCTCCATCCTGACCCTCTGCCTCATCGCACTGGGACGCTACCTC
CTCATTGCCCACCCTAAGCTTTTTCCCCAAGTTTTCAGTGCCAAGGGGATAGTGCTGGCA
CTGGTGAGCACCTGGGTTGTGGGCGTGGCCAGCTTTGCTCCCCTCTGGCCTATTTATATC
CTGGTACCTGTAGTCTGCACCTGCAGCTTTGACCGCATCCGAGGCCGGCCTTACACCACC
ATCCTCATGGGCATCTACTTTGTGCTTGGGCTCAGCAGTGTTGGCATCTTCTATTGCCTC
ATCCACCGCCAGGTCAAACGAGCAGCACAGGCACTGGACCAATACAAGTTGCGACAGGCA
AGCATCCACTCCAACCATGTGGCCAGGACTGATGAGGCCATGCCTGGTCGTTTCCAGGAG
CTGGACAGCAGGTTAGCATCAGGAGGACCCAGTGAGGGGATTTCATCTGAGCCAGTCAGT
GCTGCCACCACCCAGACCCTGGAAGGGGACTCATCAGAAGTGGGAGACCAGATCAACAGC
AAGAGAGCTAAGCAGATGGCAGAGAAAAGCCCTCCAGAAGCATCTGCCAAAGCCCAGCCA
ATTAAAGGAGCCAGAAGAGCTCCGGATTCTTCATCGGAATTTGGGAAGGTGACTCGAATG
TGTTTTGCTGTGTTCCTCTGCTTTGCCCTGAGCTACATCCCCTTCTTGCTGCTCAACATT
CTGGATGCCAGAGTCCAGGCTCCCCGGGTGGTCCACATGCTTGCTGCCAACCTCACCTGG
CTCAATGGTTGCATCAACCCTGTGCTCTATGCAGCCATGAACCGCCAATTCCGCCAAGCA
TATGGCTCCATTTTAAAAAGAGGGCCCCGGAGTTTCCATAGGCTCCAT

ORF Protein

Cloned ORF protein sequence for pFN21AB8822 Download

>KIBB8822  396 aa
MWNSSDANFSCYHESVLGYRYVAVSWGVVVAVTGTVGNVLTLLALAIQPKLRTRFNLLIA
NLTLADLLYCTLLQPFSVDTYLHLHWRTGATFCRVFGLLLFASNSVSILTLCLIALGRYL
LIAHPKLFPQVFSAKGIVLALVSTWVVGVASFAPLWPIYILVPVVCTCSFDRIRGRPYTT
ILMGIYFVLGLSSVGIFYCLIHRQVKRAAQALDQYKLRQASIHSNHVARTDEAMPGRFQE
LDSRLASGGPSEGISSEPVSAATTQTLEGDSSEVGDQINSKRAKQMAEKSPPEASAKAQP
IKGARRAPDSSSEFGKVTRMCFAVFLCFALSYIPFLLLNILDARVQAPRVVHMLAANLTW
LNGCINPVLYAAMNRQFRQAYGSILKRGPRSFHRLH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8822 Download

>pFN21AB8822  5893 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGGAACAGCTCTGACGCCAACTTCTCCTGCTA
CCATGAGTCTGTGCTGGGCTATCGTTATGTTGCAGTTAGCTGGGGGGTGGTGGTGGCTGT
GACAGGCACCGTGGGCAATGTGCTCACCCTACTGGCCTTGGCCATCCAGCCCAAGCTCCG
TACCCGATTCAACCTGCTCATAGCCAACCTCACACTGGCTGATCTCCTCTACTGCACGCT
CCTTCAGCCCTTCTCTGTGGACACCTACCTCCACCTGCACTGGCGCACCGGTGCCACCTT
CTGCAGGGTATTTGGGCTCCTCCTTTTTGCCTCCAATTCTGTCTCCATCCTGACCCTCTG
CCTCATCGCACTGGGACGCTACCTCCTCATTGCCCACCCTAAGCTTTTTCCCCAAGTTTT
CAGTGCCAAGGGGATAGTGCTGGCACTGGTGAGCACCTGGGTTGTGGGCGTGGCCAGCTT
TGCTCCCCTCTGGCCTATTTATATCCTGGTACCTGTAGTCTGCACCTGCAGCTTTGACCG
CATCCGAGGCCGGCCTTACACCACCATCCTCATGGGCATCTACTTTGTGCTTGGGCTCAG
CAGTGTTGGCATCTTCTATTGCCTCATCCACCGCCAGGTCAAACGAGCAGCACAGGCACT
GGACCAATACAAGTTGCGACAGGCAAGCATCCACTCCAACCATGTGGCCAGGACTGATGA
GGCCATGCCTGGTCGTTTCCAGGAGCTGGACAGCAGGTTAGCATCAGGAGGACCCAGTGA
GGGGATTTCATCTGAGCCAGTCAGTGCTGCCACCACCCAGACCCTGGAAGGGGACTCATC
AGAAGTGGGAGACCAGATCAACAGCAAGAGAGCTAAGCAGATGGCAGAGAAAAGCCCTCC
AGAAGCATCTGCCAAAGCCCAGCCAATTAAAGGAGCCAGAAGAGCTCCGGATTCTTCATC
GGAATTTGGGAAGGTGACTCGAATGTGTTTTGCTGTGTTCCTCTGCTTTGCCCTGAGCTA
CATCCCCTTCTTGCTGCTCAACATTCTGGATGCCAGAGTCCAGGCTCCCCGGGTGGTCCA
CATGCTTGCTGCCAACCTCACCTGGCTCAATGGTTGCATCAACCCTGTGCTCTATGCAGC
CATGAACCGCCAATTCCGCCAAGCATATGGCTCCATTTTAAAAAGAGGGCCCCGGAGTTT
CCATAGGCTCCATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT